The geographical area Eastern Vätterbranterna has since its initiation as a biosphere reserve according to UNESCO's appointment in 2012 worked towards being a model area in which sustainable development can evolve. This has been done from five sustainability perspectives: the ecological, social, economic, the perspective with broad cooperation and the learning perspective. In 2015, the UN General Assembly adopted Agenda 2030 with its seventeen global sustainability goals and associated 169 sub-goals, which the biosphere association Eastern Vätterbranterna then woven into its work. This qualitative interview study has, with the focus on the biosphere association's seven active focus areas, investigated the biosphere association of Östra Vätterbranternas work with Agenda 2030 in collaboration with the Municipality of Jönköpings and its County Government, within the association, and other community actors. Cooperation has stood as a constant point through both interviews and through one of the questions of issue, conclusions can be established that the Östra Vätterbranterna had served to perform better cooperation within themselves. The Municipality of Jönköping and its County Government looks up to the biosphere association's work with Agenda 2030 and raises collaboration between them as a major success factor, but cooperation between the focus areas in the biosphere association has not achieved its full potential. Furthermore, not all of Agenda 2030's global sustainability goals have been included, but it is something that the biosphere association is constantly working on, which continues to develop all the time, and which can be achieved with the help of other community actors. Cooperation is the concept Agenda 2030 advocates for to achieve the goals and the concept that permeates the biosphere association Eastern Vätterbranterna. / Det geografiska området Östra Vätterbranterna har sedan uppstarten som ett biosfärområde enligt UNESCOs utseende 2012 arbetat mot att vara ett modellområde där hållbar utveckling kan rota sig. Detta har de gjort utifrån fem hållbarhetsperspektiv: det ekologiska, sociala, ekonomiska, perspektivet med bred samverkan och det lärande perspektivet. 2015 antog FNs generalförsamling Agenda 2030 med dess sjutton globala hållbarhetsmål och tillhörande 169 delmål, som biosfärföreningen Östra Vätterbranterna därefter har vävt in i sitt arbete. Denna kvalitativa intervjustudie har, med inriktning på biosfärföreningens sju verksamma fokusområden undersökt biosfärföreningen Östra Vätterbranternas arbete med Agenda 2030 i samverkan med Jönköpings kommun och länsstyrelse, inom föreningen, samt andra samhällsaktörer. Samverkan har stått som en stadig punkt genom både intervjustudien och genom frågeställningar har slutsatser kunnat etableras som att Östra Vätterbranterna hade tjänat på att utföra bättre samverkan inom sig själva. Jönköpings kommun och länsstyrelse ser upp till biosfärföreningens arbete med Agenda 2030 och lyfter samverkan som sker mellan dem som en stor framgångsfaktor, men samverkan mellan fokusområdena i biosfärföreningen har inte uppnått dess fulla potential. Sedan har inte alla av Agenda 2030 globala hållbarhetsmål inkluderats, men det är någonting biosfärföreningen ständigt arbetar med, som fortsätter att utvecklas hela tiden och som med hjälp av andra samhällsaktörer kommer kunna uppnås. Samverkan är det koncept Agenda 2030 förespråkar för att kunna nå målen och det koncept som genomsyrar biosfärföreningen Östra Vätterbranterna.

MSc (Zoology) / Department of Zoology / See the attached abstract below

The thesis is based on the idea of extension the Krkonoše/Karkonosze transboundary biosphere reserve by Jizera Mountains territory. Biosphere reserves are established within the Man and the Biosphere Programme of UNESCO. According to the Madrid Action Plan it is necessary for the sake of appropriate functioning of biosphere reserve to involve all the stakeholders, including non-governmental organizations. Using semi-structured interviews with the representatives of the Jizera Mountains non-governmental organizations I therefor asked them about their opinion of the extension of the Krkonoše/Karkonosze transboundary biosphere reserve by this area and their willingness to participate in cooperation within the biosphere reserve as well. I investigated their sustainable development capacity, which in the same time represents their possible contribution to the biosphere reserve. The research shows that non-governmental organizations accepts the idea of the extension of the Krkonoše/Karkonosze transboundary biosphere reserve by Jizera Mountains. Together with it their potential for backing the appropriate functioning of biosphere reserve in this territory was proven. Keywords Man and the Biosphere Programme of UNESCO, biosphere reserve, sustainable development, non-governmental organizations, Jizera...

Metabolically active microbial communities are present in a wide range of subsurface environments. Techniques like enumeration of microbial cells, activity measurements with radiotracer assays and the analysis of porewater constituents are currently being used to explore the subsurface biosphere, alongside with molecular biological analyses. However, many of these techniques reach their detection limits due to low microbial activity and abundance. Direct measurements of microbial turnover not just face issues of insufficient sensitivity, they only provide information about a single specific process but in sediments many different process can occur simultaneously. Therefore, the development of a new technique to measure total microbial activity would be a major improvement. A new tritium-based hydrogenase-enzyme assay appeared to be a promising tool to quantify total living biomass, even in low activity subsurface environments. In this PhD project total microbial biomass and microbial activity was quantified in different subsurface sediments using established techniques (cell enumeration and pore water geochemistry) as well as a new tritium-based hydrogenase enzyme assay. By using a large database of our own cell enumeration data from equatorial Pacific and north Pacific sediments and published data it was shown that the global geographic distribution of subseafloor sedimentary microbes varies between sites by 5 to 6 orders of magnitude and correlates with the sedimentation rate and distance from land. Based on these correlations, global subseafloor biomass was estimated to be 4.1 petagram-C and ~0.6 % of Earth's total living biomass, which is significantly lower than previous estimates. Despite the massive reduction in biomass the subseafloor biosphere is still an important player in global biogeochemical cycles. To understand the relationship between microbial activity, abundance and organic matter flux into the sediment an expedition to the equatorial Pacific upwelling area and the north Pacific Gyre was carried out. Oxygen respiration rates in subseafloor sediments from the north Pacific Gyre, which are deposited at sedimentation rates of 1 mm per 1000 years, showed that microbial communities could survive for millions of years without fresh supply of organic carbon. Contrary to the north Pacific Gyre oxygen was completely depleted within the upper few millimeters to centimeters in sediments of the equatorial upwelling region due to a higher supply of organic matter and higher metabolic activity. So occurrence and variability of electron acceptors over depth and sites make the subsurface a complex environment for the quantification of total microbial activity. Recent studies showed that electron acceptor processes, which were previously thought to thermodynamically exclude each other can occur simultaneously. So in many cases a simple measure of the total microbial activity would be a better and more robust solution than assays for several specific processes, for example sulfate reduction rates or methanogenesis. Enzyme or molecular assays provide a more general approach as they target key metabolic compounds. Since hydrogenase enzymes are ubiquitous in microbes, the recently developed tritium-based hydrogenase radiotracer assay is applied to quantify hydrogenase enzyme activity as a parameter of total living cell activity. Hydrogenase enzyme activity was measured in sediments from different locations (Lake Van, Barents Sea, Equatorial Pacific and Gulf of Mexico). In sediment samples that contained nitrate, we found the lowest cell specific enzyme activity around 10^(-5) nmol H_(2) cell^(-1) d^(-1). With decreasing energy yield of the electron acceptor used, cell-specific hydrogenase activity increased and maximum values of up to 1 nmol H_(2) cell^(-1) d^(-1) were found in samples with methane concentrations of >10 ppm. Although hydrogenase activity cannot be converted directly into a turnover rate of a specific process, cell-specific activity factors can be used to identify specific metabolism and to quantify the metabolically active microbial population. In another study on sediments from the Nankai Trough microbial abundance and hydrogenase activity data show that both the habitat and the activity of subseafloor sedimentary microbial communities have been impacted by seismic activities. An increase in hydrogenase activity near the fault zone revealed that the microbial community was supplied with hydrogen as an energy source and that the microbes were specialized to hydrogen metabolism. / Mikrobielle Gesellschaften und ihre aktiven Stoffwechselprozesse treten in einer Vielzahl von Sedimenten unterschiedlichster Herkunft auf. In der Erforschung dieser tiefen Biosphäre werden derzeit Techniken wie Zellzählungen, Aktivitätsmessungen mit Radiotracer-Versuchen und Analysen der Porenwasserzusammensetzung angewendet, darüber hinaus auch molekularbiologische Analysen. Viele dieser Methoden stoßen an ihre Nachweisgrenze, wenn Sedimente mit geringer Zelldichte und mikrobieller Aktivität untersucht werden. Bei der Untersuchung von Stoffwechselprozessen mit herkömmlichen Techniken kommt dazu, dass von mehreren Prozessen, die zeitgleich ablaufen können, jeweils nur einer erfasst wird. Deswegen wäre die Entwicklung einer neuartigen Messtechnik für die gesamte mikrobielle Aktivität ein wesentlicher Fortschritt für die Erforschung der tiefen Biosphäre. Ein vielversprechender Ansatz, um die gesamte lebende Biomasse auch in Proben mit geringer Aktivität zu bestimmen, ist eine Hydrogenase-Enzym-Versuchsanordnung mit Tritium als quantifizierbarer Messgröße. In dieser Doktorarbeit wurde die gesamte mikrobielle Biomasse und Aktivität von unterschiedlichen Sedimentproben einerseits mit herkömmlichen Methoden (Zellzählungen, Analyse der Porenwasserzusammensetzung) als auch mit einer neu entwickelten Hydrogenase-Enzym-Versuchsanordnung quantifiziert. Mit einer großen Anzahl eigener Zellzählungsdaten von Sedimenten aus dem Äquatorialpazifik und dem Nordpazifik und ergänzenden publizierten Daten konnte gezeigt werden, dass Zellzahlen sich in ihrer globalen geographischen Verteilung je nach Bohrlokation um 5 bis 6 Größenordnungen unterscheiden. Dabei bestehen Korrelationen zur Sedimentationsrate und zur Entfernung zum Land, mit deren Hilfe sich die Gesamtbiomasse in Tiefseesedimenten zu 4,1 Petagramm-C abschätzen lässt. Das entspricht ~0,6 % der Gesamtbiomasse der Erde und ist damit erheblich weniger als in früheren Schätzungen angegeben. Trotz der Korrektur auf diesen Wert spielt die Biomasse der tiefen Biosphäre weiterhin eine erhebliche Rolle in biogeochemischen Kreisläufen. Um die Zusammenhänge zwischen Aktivität der Mikroben, der Häufigkeit ihres Auftretens und Zustrom von organischem Material zu verstehen, wurde eine Expedition ins Auftriebsgebiet des Äquatorialpazifiks und zum nordpazifischen Wirbel durchgeführt. Daten der Sauerstoffaufnahme in Sedimenten des nordpazifischen Wirbels, die mit Sedimentationsraten von 1 mm pro 1000 Jahren abgelagert werden, zeigen, dass mikrobielle Gesellschaften über Millionen von Jahren ohne Zufuhr von frischem organischen Kohlenstoff überleben konnten. Im Gegensatz zum nordpazifischen Wirbel wird in Sedimenten des äquatorialpazifischen Auftriebsgebiets Sauerstoff bei höherer mikrobieller Aktivität und Verfügbarkeit organischer Verbindungen oberflächennah in den ersten Milli- bis Zentimetern komplett umgesetzt. Auftreten und Variabilität von Elektronenakzeptoren nach Tiefe und Bohrlokation machen die tiefe Biosphäre zu einer komplexen Umgebung für die Quantifizierung der gesamten mikrobiellen Aktivität. Aktuelle Studien zeigen das verschiedene Elektronenakzeptorprozesse gleichzeitig ablaufen können, obwohl man bisher davon ausgegangen war, dass diese sich thermodynamisch ausschließen. In vielen Fällen wäre also eine einfache Methode zur Messung der gesamten mikrobiellen Aktivität eine bessere und verlässlichere Lösung aktueller Analyseaufgaben als Messungen mehrerer Einzelprozesse wie beispielsweise Sulfatreduktion und Methanogenese. Enzym-oder Molekular-Versuchsanordnungen sind ein prozessumfassender Ansatz, weil hier Schlüsselkomponenten der Stoffwechselprozesse untersucht werden. Das Hydrogenase-Enzym ist eine solche Schlüsselkomponente und in Mikroben allgegenwärtig. Deshalb kann die Quantifizierung seiner Aktivität mit der neu entwickelten Hydrogenase-Enzym-Versuchsanordnung als Parameter für die gesamte mikrobielle Aktivität der lebenden Zellen verwendet werden. Hydrogenase-Aktivitäten wurden in Sedimenten unterschiedlicher Lokationen (Vansee, Barentssee, Äquatorialpazifik, und Golf von Mexico) gemessen. In Sedimentproben, die Nitrat enthielten, haben wir mit ca. 10^(-5) nmol H_(2) cell^(-1) d^(-1) die geringste zellspezifische Hydrogenase-Aktivität gefunden. Mit geringerem Energiegewinn des genutzten Elektronenakzeptors steigt die zellspezifische Hydrogenase-Aktivität. Maximalwerte von bis zu 1 nmol H_(2) cell^(-1) d^(-1) wurden in Sedimentproben mit >10 ppm Methankonzentration gefunden. Auch wenn die Hydrogenase-Aktivität nicht direkt in die Umsatzrate eines spezifischen Prozesses konvertierbar ist, können zellspezifische Aktivitätsfaktoren verwendet werden, um die metabolisch aktive Mikrobenpopulation zu quantifizieren. In einer weiteren Studie mit Sedimenten des Nankai-Grabens zeigen Daten der Zelldichte und der Hydrogenase-Aktivität einen Einfluss von seismischen Ereignissen auf Lebensraum und Aktivität der mikrobiellen Gesellschaften. Ein Anstieg der Hydrogenase-Aktivität nahe der Verwerfungszone machte deutlich, dass die mikrobiellen Gesellschaften mit Wasserstoff als Energiequelle versorgt wurden und dass die Mikroben auf einen Wasserstoff-Stoffwechsel spezialisiert waren.

This thesis describes the use of different DNA sequencing technologies to investigate the structure and function of microbial communities in two extreme environments, boreal acid sulfate soil and the terrestrial deep biosphere. The first of the two investigated environments was soils containing un-oxidized metal sulfides that are termed ‘potential acid sulfate soil’ (PASS) materials. If these materials are exposed to atmospheric oxygen by either natural phenomena (e.g., land uplift) or human activities (e.g., drainage) then the metal sulfides become oxidized and the PASS becomes acidic and is defined as an ‘acid sulfate soil’ (ASS). The resulting acid and metal release from metal sulfide oxidation can lead to severe environmental damage. Although acidophilic microorganisms capable of catalyzing acid and metal release have been identified from many sulfide mineral containing environments, the microbial community of boreal PASSs/ASSs remains unclear. This study investigated the physicochemical and microbial characteristics of PASSs and ASSs from the Risöfladan experimental field in Vasa, Finland. Sanger sequencing of 16S rRNA gene sequences of microorganisms present in the PASSs and ASSs were mostly assigned to acidophilic species and environmental clones previously identified from acid- and metal-contaminated environments. Enrichment cultures inoculated from the ASS demonstrated that the acidophilic microorganisms were responsible for catalyzing acid and metal release from PASSs/ASSs. Lastly, the study investigated how to mitigate metal sulfide oxidation and the concomitant formation of sulfuric acid by treating ASSs in situ with CaCO3 or Ca(OH)2 suspensions. The DNA sequencing still identified acidophilic microorganisms after the chemical treatments. However, the increased pH during and after treatment suggested that the activity of the acidophiles might be inhibited. This study was the first to identify the microbial community present in boreal PASSs/ASSs and suggested that treatment with basic compounds may inhibit microbial catalysis of metal sulfide dissolution. The second studied environment was the deep, dark terrestrial subsurface that is suggested to be both extremely stable and highly oligotrophic. Despite the scarcity of carbon and energy sources, the deep biosphere is estimated to constitute up to 20% of the total biomass on earth and thus, represents the largest microbial ecosystem. However, due to the difficulties of accessing this environment and our inability to cultivate the indigenous microbial populations, details of the diversity and metabolism of these communities remain largely unexplored. This study was carried out at Äspö Hard Rock Laboratory, Sweden and utilized second-generation sequencing to identify the taxonomic composition and genetic potential of planktonic and biofilm populations. Community DNA sequencing of planktonic cells from three water types at varied age and depth (‘modern marine’, ‘undefined mixed’, and ‘old saline’) showed the existence of ultra-small cells capable of passing through a 0.22 μm filter that were phylogenetically distinct communities from the >0.22 μm fraction. The reduced cell size and/or genome size suggested a potential adaptation to the oligotrophic environment in the terrestrial deep biosphere. The identified planktonic communities were dominated by Proteobacteria, Candidate divisions, unclassified archaea, and unclassified bacteria. Functional analysis of the assembled genomes showed that the planktonic population from the shallow modern marine water demonstrated a predominantly anaerobic and heterotrophic lifestyle. In contrast, the deeper, old saline water was more closely aligned with the hypothesis of a hydrogen-driven deep biosphere. Metagenomic analysis of subsurface biofilms from ‘modern marine’ and ‘old saline’ water types suggested only a subset of populations were involved in initial biofilm formation. The identified biofilm populations from both water types were distinct from the planktonic community and were suggested to be dominated by hydrogen fed, chemolithoautotrophic and diazotrophic populations.

A study was undertaken to explore relationships between wetland characteristics which make them efficient water purifiers versus their ability to serve as wildlife habitat. The effects of designing constructed wetlands for improved habitat on water treatment efficiencies were quantified. Results indicate that some sacrifice in treatment efficiency is required and that the degree of efficiency reduction is dependant upon pollutant loading rates. However, sacrifice in efficiency is much smaller than increase in habitat quality, and can be offset by increasing wetland area. A practical, theoretical application was then attempted.

Tourism is one of the progressively evolving fields and due to the influence of globalization has become attractive and easily accessible matter for a broad group of people across the continents. Economical aspects of this perspective branch supported generally uncritical perception of phenomena called tourism. In recent years, however the negative impact of mass tourism became the subject of discussion and efforts are being made to find alternative forms which respect the environment and contribute to the development of the visited regions. The aim of thesis is study of ecotourism and biospheres reserves. Both concepts contribute to the achievement of sustainable development in Latin America. Biosphere Reserves based on the effort of harmonious coexistence between man and nature are the ideal environment for the application of the principles of sustainability. Ecotourism in Latin American practice is analyzed on the basis of case studies from Costa Rica and the Galapagos Islands. Operation, management and benefits of biosphere reserves for the region demonstrate examples of Guatemala and Costa Rica. Keywords: biosphere reserve; ecotourism; Latin America; sustainable development

Environmental monitoring initiatives are typically conceived as strictly scientific affairs designed to provide support for managerial decision-making; as a consequence most initiatives are centered on a formal mandate or an overarching mission statement that provides direction for monitoring activity. But official frameworks tend to marginalize lay perspectives as experts pursue disciplinary rigor at the expense of public input, a situation not in keeping with the spirit of the biosphere reserve concept. This thesis argues that an alternative design approach that reaches beyond scientists and resource managers is necessary. Environmental monitoring under an ecosystem approach is subject to scientific, social, and bureaucratic demands that defy easy disentanglement. A medley of factors influence how data are collected, interpreted, and used; neglect of these 'soft' dimensions runs the risk of failing to win the enduring support of stakeholders. There is a need to coordinate activity and to partially align multiple perspectives-this is the 'soft underbelly' of integrated monitoring that gets short shrift in most designs. While there is much monitoring being done in and around the Long Point World Biosphere Reserve, there is little coordination among monitoring groups and no obvious way to combine disparate data sets in a meaningful way. This thesis describes the elements of a locally-sensible framework for monitoring practice that is mainly concerned with trying to make sense of confusing and ambiguous situations; it strives to integrate the 'why', 'what', and 'how' of monitoring in as transparent a manner as possible by crafting 'boundary objects' that help to congeal understanding and provide centers of coordination. Using principles of participatory design in the soft-systems tradition, the overall intent is to primarily support sense-making, not decision-making; to generate searching questions, not final solutions; to facilitate learning, not control.

Environmental monitoring initiatives are typically conceived as strictly scientific affairs designed to provide support for managerial decision-making; as a consequence most initiatives are centered on a formal mandate or an overarching mission statement that provides direction for monitoring activity. But official frameworks tend to marginalize lay perspectives as experts pursue disciplinary rigor at the expense of public input, a situation not in keeping with the spirit of the biosphere reserve concept. This thesis argues that an alternative design approach that reaches beyond scientists and resource managers is necessary. Environmental monitoring under an ecosystem approach is subject to scientific, social, and bureaucratic demands that defy easy disentanglement. A medley of factors influence how data are collected, interpreted, and used; neglect of these 'soft' dimensions runs the risk of failing to win the enduring support of stakeholders. There is a need to coordinate activity and to partially align multiple perspectives-this is the 'soft underbelly' of integrated monitoring that gets short shrift in most designs. While there is much monitoring being done in and around the Long Point World Biosphere Reserve, there is little coordination among monitoring groups and no obvious way to combine disparate data sets in a meaningful way. This thesis describes the elements of a locally-sensible framework for monitoring practice that is mainly concerned with trying to make sense of confusing and ambiguous situations; it strives to integrate the 'why', 'what', and 'how' of monitoring in as transparent a manner as possible by crafting 'boundary objects' that help to congeal understanding and provide centers of coordination. Using principles of participatory design in the soft-systems tradition, the overall intent is to primarily support sense-making, not decision-making; to generate searching questions, not final solutions; to facilitate learning, not control.

The implementation of UNESCO Biosphere Reserves (BR) in Canada is strongly dependent on grassroots community-based support and understanding. The recent calls for the Oak Ridges Moraine and adjacent Greenbelt lands (ORMGB) to be designated a BR require that a communications strategy be created to garner local support. Taking into consideration complex systems theory, this study looked to build a communications framework that combined higher-scale social organizing literature like social movement and environmental campaigns more detail-focused group dynamics and strategic communications research. Applying this framework to the ORMBG landscape revealed key target audience groups and messaging for the BR communications strategy.