Age determination of bottlenose dolphins (Tursiops truncatus) is a critical tool in understanding their health. There are many methods of aging dolphins including morphometrics, counting growth layer groups (GLGs) of teeth, comparing dentin to pulp cavity ratio on dental radiographs, analysis of the pectoral flipper radiographs, and epigenetic testing. The most common method for aging toothed cetaceans is the counting of GLGs in the teeth. Since there are many challenges associated with aging dolphins based on the counting of GLGs, a processing technique that results in better resolution of GLGs is needed. This is a two-part study that first compares different decalcification and staining techniques that results in the process that best highlights GLGs. Secondly, this study compares the results of aging via GLGs and pectoral flipper radiographs in 47 bottlenose dolphins. This study suggests aging by pectoral flipper radiograph assessment can more accurately classify dolphins by age class.

Bottlenose dolprlin (Tursiops trunratus) whistles are currently studied by subjective visual comparison of whistle spectrograms. This thesis describes the novel use of stochastic modeling to automate the comparison of dolphin whistles and to yield an objective, quantitative measure of whistle similarity. The relationship of bottlenose dolphin whistle production to a model of human speech production is discussed, providing a basis for the use of human speech recognition techniques for creating whistle models. Discrete hidden Markov models based on vector quantization of linear prediction coefficients are used to create whistle models based on statistical information derived from a sample set of dolphin whistles. Whistle model comparison results are presented indicating that evaluation of bottlenose dolphin whistles via hidden Markov modeling provides an objective measure of similarity between whistles. The results also demonstrate that hidden Markov models provide robustness against the effects of temporal and frequency variance in the comparison of whistles. The extensibility of stochastic modeling techniques to other animal vocalizations is discussed and possibilities for further work in areas such as the determination of possible structural components, similar to phonemes in human speech, is provided. / Master of Science

The ranging behaviour, habitat preferences, genetic structure, and demographic parameters of bottlenose dolphins living in the Azores were studied using data collected from 1999 to 2004. Only 44 dolphins out of 966 identified were frequently sighted within and between years and showed strong site fidelity. The remaining individuals were either temporary migrants from within or outside the archipelago, or transients. Estimates of home range size were three times larger than previously reported for this species, possibly as a result of the lower availability of food resources. Mitochondrial DNA sequences showed very high gene and nucleotide diversity. There was no evidence of population structuring within the Azores. The Azorean population was not differentiated from the pelagic population of the Northwest Atlantic, suggesting the "unproductive" waters of the Atlantic do not constitute a barrier to dispersal. Population size, survival and temporary emigration rates were estimated using open-population models and Pollock's robust design. A few hundreds of dolphins occur in the area on a given year, though the majority should use it temporarily, as suggested by the high emigration rates. Bottlenose dolphins preferentially used shallow areas with high bottom relief. Temporal and spatial persistence of dolphin-habitat associations documented in this study further supports the idea of a close relationship between certain bathymetric features and important hydrographic processes and suggests the occurrence of prey aggregations over these areas may be, to some extent, predictable. Several results of this study suggest there are no reasons for concern about the status of this population. Yet, the resident group may be negatively affected by increasing pressure from the whale watching activity. Although the proposed Marine Park constitutes important habitat for resident dolphins, at present, the area is clearly insufficient to satisfy their spatial requirements and its conservation value may be limited.

Thesis (Ph. D.)--Joint Program in Biological Oceanography (Massachusetts Institute of Technology, Dept. of Biology; and the Woods Hole Oceanographic Institution), 1999. / Includes bibliographical references. / Odontocetes are assumed to use echolocation for navigation and foraging, but neither of these uses of biosonar has been conclusively demonstrated in free-ranging animals. Many bats are known to use echolocation throughout foraging sequences, changing the structure and timing of clicks as they progress towards prey capture. For odontocetes, however, we do not know enough about their foraging behavior to describe such sequences. To conduct detailed behavioral observations of any subject animal, the observer must be able to maintain continuous visual contact with the subject for a period commensurate with the duration of the behavior(s) of interest. Behavioral studies of cetaceans, which spend approximately 95% of their time below the water's surface, have been limited to sampling surface behavior except in special circumstances, e.g. clear-water environments, or with the use of technological tools. I addressed this limitation through development of an observation platform consisting of a remote controlled video camera suspended from a tethered airship with boat-based monitoring, adjustment, and recording of video. The system was used successfully to conduct continuous behavioral observations of bottlenose dolphins in the Sarasota Bay, FL area. This system allowed me to describe previously unreported foraging behaviors and elucidate functions for behaviors already defined but poorly understood. Dolphin foraging was modeled as a stage-structured sequence of behaviors, with the goal-directed feeding event occurring at the end of a series of search, encounter, and pursuit behaviors. The behaviors preceding a feeding event do not occur in a deterministic sequence, but are adaptive and plastic. A single-step transition analysis beginning with prey capture and receding in time has identified significant links between observed behaviors and demonstrated the stage-structured nature of dolphin foraging. Factors affecting the occurrence of specific behaviors and behavioral transitions include mesoscale habitat variation and individual preferences. The role of sound in foraging, especially echolocation, is less well understood than the behavioral component. Recent studies have explored the use of echolocation in captive odontocete foraging and presumed feeding in wild animals, but simultaneous, detailed behavioral and acoustic observations have eluded researchers. The current study used two methods to obtain acoustic data. The overhead video system includes two towed hydrophones used to record 'ambient' sounds of dolphin foraging. The recordings are of the 'ambient' sounds because the source of the sounds, i.e. animal, could not be localized. Many focal follows, however, were conducted with single animals, and from these records the timing of echolocation and other sounds relative to the foraging sequence could be examined. The 'ambient' recordings revealed that single animals are much more vocal than animals in groups, both overall and during foraging. When not foraging, single animals vocalized at a rate similar to the per animal rate in groups of>=2 animals. For single foraging animals, the use of different sound types varies significantly by the habitat in which the animal is foraging. These patterns of use coupled with the characteristics of the different sound types suggest specific functions for each. The presence of multiple animals in a foraging group apparently reduces the need to vocalize, and potential reasons for this pattern are discussed. In addition, the increased vocal activity of single foraging animals lends support to specific hypotheses of sound use in bottlenose dolphins and odontocetes in general. The second acoustic data collection method records sounds known to be from a specific animal. An acoustic recording tag was developed that records all sounds produced by an animal including every echolocation click. The tag also includes an acoustic sampling interval controller and a sensor suite that measures pitch, roll, heading, and surfacing events. While no foraging events occurred while an animal was wearing an acoustic data logger, the rates of echolocation and whistling during different activities, e.g. traveling, were measured. / by Douglas Paul Nowacek. / Ph.D.

Two sympatric species of dolphins (Atlantic spotted dolphins, Stenella frontalis and Atlantic bottlenose dolphins, Tursiops truncatus) have been long-term residents to Little Bahama Bank. This study assessed whether there was a change in residence patterns or diurnal foraging depths of bottlenose dolphins following a large emigration event in the resident spotted dolphin community on this sandbank. Photo-identification was used to identify individual bottlenose dolphins and compare pooled years before (2010-2012) and after (2013-2015) the spotted dolphin emigration. The identified community size and overall residency of the bottlenose dolphins remained similar, although two bottlenose dolphins emigrated over deep water to the site spotted dolphins emigrated. Bottlenose dolphins diurnally fed in shallower water but remained in the same geographic foraging locations. Reasons remain unknown for this depth change, but potential changes in the productivity of primary bottlenose dolphin foraging habitats or reduction of spotted dolphins from shallower depths remain possibilities. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2017. / FAU Electronic Theses and Dissertations Collection

Mass mortalities of Florida manatees (Trichechus manatus latirostris) and bottlenose dolphins (Tursiops truncatus) have been attributed to brevetoxins produced by the Florida red tide dinoflagellate Karenia brevis. The multiple routes through which marine mammals can be exposed to brevetoxins have complicated efforts to understand the mechanisms that lead to mass mortality events. In spring of 2002, 34 endangered Florida manatees died in southwest Florida, and in spring of 2004, 107 bottlenose dolphins died in the Florida Panhandle. These events provided unique opportunities to make clear connections between ingested brevetoxins and marine mammal mortalities without the confounding issues of concurrent exposure through direct contact or inhalation. Prior to 2002, the accumulation of brevetoxins on or in seagrass had never been previously reported, and the delayed or chronic exposure of manatees to brevetoxins through seagrass was not recognized as a threat. Brevetoxins were shown to persist in association with seagrass at high levels for weeks and at lower levels for months in the absence of K. brevis. Analyses of the epiphytes and detritus on the surface of the seagrass leaves as well as of the cleaned seagrass leaves and rhizomes revealed that during a K. brevis bloom as much as half of the toxin present in the seagrass may be associated with the leaves themselves, while after a bloom, the majority of the toxin present is associated with the epiphytes. The 2004 mass mortality of bottlenose dolphins in the Florida Panhandle clearly indicated that fish have the potential to vector brevetoxins to higher tropic levels. Analyses of fish collected live from St. Joseph Bay and southwest Florida revealed that brevetoxin accumulation in fish is a common occurrence. Planktivorous clupeid fish are capable of accumulating high concentrations of brevetoxins within their viscera, and their movement can result in spatial separation of a bloom and animal exposure. Sciaenid species and pinfish also accumulated brevetoxins but to a lower extent. These fish, as well as other omnivorous and piscivorous species, may retain brevetoxins in their tissues at significant concentrations after a bloom has dissipated, which may lead to temporal separation of blooms and animal exposure.

The objective of this research was to examine bottlenose dolphin (Tursiops truncatus) habitat use in the Indian River Lagoon (IRL) based on monthly relocation of photo-identified individuals, prey availability and environmental factors from 2003-2015. We focused on the variation of spatial and temporal abiotic and biotic factors and their influence on bottlenose dolphin habitat use patterns. Harbor Branch Oceanographic Institute (HBOI) conducted monthly photo-identification surveys along the length of the IRL and GPS locations of photographed dolphins were collected at the time of surveying. Stratified random samples of prey and environmental variables were collected monthly by the Florida Fish and Wildlife Conservation Commission (FWC) as part of the Fisheries-Independent Monitoring (FIM) program. Kernel density estimation was used to determine the magnitude-per-unit area of dolphins across a continuous raster surface of the IRL by wet and dry seasons each year, the values of which were used as a response variable in Classification and regression tree (CART) analyses with FIM fish community and environmental factors as predictors. Understanding how dolphins respond to environmental factors over time in the IRL could be used to predict future responses in estuaries and prioritize conservation and restoration actions. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2019. / FAU Electronic Theses and Dissertations Collection

Thesis (Ph. D.)--Simon Fraser University, 2001. / Includes bibliographical references.

Seasonality of reproduction in common dolphins (Delphinus delphis), bottlenosed dolphins (Tursiops aduncus), striped dolphins (Stenella coeruleoalba) and humpbacked dolphins (Sousa chinensis) was examined using standard light microscope techniques. In the males four stages of testicular development could be identified. Individuals less than 2.26m in length and 105kg in mass had testes that contained small seminiferous tubules (0.048mm ± O.Olmm in diameter) and were classified either as juveniles (gonocytes present) or inactive (no gonocytes). Adults whose testes contained spermatozoa were said to be in late spermatogenesis while those whose testes contained no spermatids or spermatozoa were referred to as being in early spermatogenesis. In the males of D. delphis, T. aduncus and S. chinensis, late spermatogenesis occurred throughout the year and there was no significant difference in the monthly mean seminiferous tubule diameters (p > 0.05) within species. I conclude therefore that in these three species spermatogenesis is aseasonal. No conclusions about seasonality of spermatogenesis could be made for S. coeruleoalba owing to the small sample size. Large Graafian follicles occurred in the ovaries of all four species in most months of the year. However, the presence of Graafian follicles can not be taken as an indication of timing of reproduction since they may be remnants of follicles that have not yet degenerated. In D. delphis, T. aduncus and S. coeruleoalba luteal bodies (corpora albicans or corpora lutea) occurred in most months of the year. Active corpora lutea will be present in all months, in some members of the population, since gestation is approximately 12 months. The sperm structure of D. delphis was examined by scanning electron microscopy. The sperm of D. delphis is essentially similar to that described for two other species of Cetacea (Physeter catodon and Tursiops aduncus), having an ellipsoidally shaped head and a short mid-piece with nine mitochondria. The sperm dimensions for D. delphis were head length, 4.4pm; head width, 2.0pm and mid-piece length, 2.4pm. An analysis of foetal age in D. delphis showed that the majority of the foetuses were conceived in January with birth occurring the following summer (December), suggesting that reproduction is seasonal. Neonates of T. aduncus were found throughout the year suggesting aseasonal reproduction. Foetal material was not available for S. coeruleoalba and S. chinensis. Seasonality of reproduction in the four species of dolphins studied has been discussed in relation to feeding, migration and worldwide distribution of the species.

Coastal dolphins on the South African east coast are threatened by degradation and loss of habitat as a result of increasing coastal development, industrial effluent and agricultural runoff. In addition, dolphins off the coast of KwaZulu-Natal have, for more than four decades, been heavily exploited through unchecked incidental capture in shark nets set at 45 beaches. In light of the high rate of mortality and apparent depletion of both species, the persistence of bottlenose (Tursiops truncatus) and humpback (Sousa chinensis) dolphins in that region has been questioned. Genetic variation in south east African dolphin populations was determined as a means of assessing the fitness of the populations and their resilience to demographic disturbances. Furthermore, in order to determine the effects of continued mortality on the KwaZulu-Natal subpopulations, it was necessary to determine whether they are open or closed to immigration from the adjacent East Cape region, which represents a relatively unstressed region, characterised by a lack of shark nets and less intensive coastal activities. Genetic variation and differentiation in the maternal genome was assessed by determining the sequence of the first 400 bases of the mtDNA control region in bottlenose and humpback dolphins from KwaZulu-Natal and the East Cape. Nuclear variation and differentiation was estimated at six microsatellite loci and compared with earlier estimates determined from allozyme electrophoresis. Random amplified polymorphic DNA (RAPD) was assessed as a means of identifying population subdivisions and diagnostic population markers. Both bottlenose and humpback dolphins on the South African east coast are characterised by low nuclear and organellar genetic variation, consistent with a possible genetic bottleneck, the inferred date of which coincides with the onset of the last glacial period. Genetic variation in South African bottlenose dolphins was lower than that reported elsewhere for the species, while an intraspecific comparison supported lower genetic variation in South African humpback dolphins than in humpback dolphins sampled off Hong Kong. An analysis of molecular variance (AMOVA), performed on mtDNA haplotype frequency data indicated, for both species, significant genetic subdivision, concordant with geographic location. The data suggested female bottlenose dolphins demonstrate regional philopatry, displaying limited movement between KwaZulu-Natal and the East Cape. Female humpback dolphins tend towards strict local philopatry, with significant maternal differentiation evident both within and between regional subdivisions. Differentiation in microsatellite allele frequencies was also demonstrated between KwaZulu-Natal and the East Cape for both species, suggesting that the movement of male bottlenose and humpback dolphins may also be restricted. Nonetheless, considerably higher nuclear gene flow estimates suggested that males of both species represent the principal vectors of gene dispersal. The implications of historically low genetic variability and population subdivision in South African dolphins are important in view of the current rate of mortality in KwaZulu-Natal. The persistence of coastal dolphin populations relies on their ability to recover following a bottleneck event. Continued removal of demographically important age-sex classes such as occurs in shark nets, may not only further reduce the genetic variation, but would ultimately deplete dolphin populations in KwaZulu-Natal beyond a sustainable number, resulting in eventual local extinction. The differentiation of the two regions implies that, in the event of local extinction occurring, dolphins, particularly females, from adjacent regions will not readily re-colonise the area. This would result in fragmentation of the south east African populations and ensure reproductive isolation from neighbouring populations on the east African coast.