Author also know as S M Loveday / Fresh bakery products have a very short shelf life, which limits the extent to which manufacturing can be centralised. Frozen doughs are relatively stable and can be manufactured in large volumes, distributed and baked on-demand at the point of sale or consumption. With appropriate formulation and processing a shelf life of several months can be achieved.Shelf life is limited by a decline in proofing rate after thawing, which is attributed to a) the dough losing its ability to retain gas and b) insufficient gas production, i.e. yeast activity. The loss of shelf life is accelerated by delays between mixing and freezing, which allow yeast cells the chance to ferment carbohydrates.This work examined the reasons for insufficient gas production after thawing frozen dough and the effect of pre-freezing fermentation on shelf life. Literature data on yeast metabolite dynamics in fermenting dough were incomplete. In particular there were few data on the accumulation of ethanol, a major fermentation end product which can be injurious to yeast.Doughs were prepared in a domestic breadmaker using compressed yeast from a local manufacturer and analysed for glucose, fructose, sucrose, maltose and ethanol. Gas production after thawing declined within 48 hours of frozen storage. This was accelerated by 30 or 90 minutes of fermentation at 30;C prior to freezing.Sucrose was rapidly hydrolysed and yeast consumed glucose in preference to fructose. Maltose was not consumed while other sugars remained. Ethanol, accumulated from consumption of glucose and fructose, was produced in approximately equal amounts to CO2, indicating that yeast cells metabolised reductively.Glucose uptake in fermenting dough followed simple hyperbolic kinetics and fructose uptake was competitively inhibited by glucose. Mathematical modelling indicated that diffusion of sugars and ethanol in dough occurred quickly enough to eliminate solute gradients brought about by yeast metabolism.

Thesis (PhD) -- University of Western Sydney, 2003. / A thesis submitted to the University of Western Sydney in partial fulfillment of the requirements for the degree of Doctor of Philosophy. Includes references pp.191 - 202, and appendices.

A enzima lipoxigenase através da farinha integral de soja é amplamente utilizada na panificação com objetivos de branqueamento e melhora da reologia. Com base nesse pressuposto, o objetivo inicial deste estudo foi de observar a ação oxidante dessa enzima na presença de dois outros oxidantes (ácido ascórbico e peróxido de benzoíla) nas propriedades reológicas e sensoriais de pães. Sete misturas com diferentes combinações dos três compostos foram elaboradas utilizando-se um delineamento experimental do tipo \"centroide-simplex\", sendo aplicadas à farinha de trigo para o preparo de pães. A enzima apresentou sinergia com o ácido ascórbico na elasticidade da massa, sugerindo uma inter-relação bioquímica entre os dois compostos no fortalecimento da matriz protéica do glúten. A mesma sinergia entre a lipoxigenase da soja e o acido ascórbico foi observada na redução da tonalidade amarela dos pães medida instrumentalmente. A partir desses resultados, buscou-se avaliar a ação da enzima frente a variações tanto na força da farinha como no tempo de fermentação, reproduzindo as condições reais de processamento. Desta vez, um planejamento experimental do tipo Box-Behnken com três fatores (atividade da enzima, força da farinha e tempo de fermentação) em três níveis de variação, foi aplicado no preparo das amostras. Os resultados mostraram que a lipoxigenase da soja (fração 1) é uma enzima oxidante de ação rápida e seu efeito no branqueamento dos carotenóides da farinha de trigo exibiu interação positiva tanto com a força da farinha como o tempo de fermentação. Respostas reológicas e sensoriais decorrentes da ação conjunta de diferentes oxidantes e condições de processamento em pães foram pela primeira vez descritas através de modelos polinomiais com capacidade preditiva. / Soy lipoxygenase enzyme through soy flour is widely used in breadmaking for bleaching and rheological improvement. Based on this fact, the initial objective of this study was to observe the enzymes\' action in the presence of two other oxidants (ascorbic acid and benzoyl peroxide) in the rheological and sensory properties of breads. Seven mixtures with different combinations of the three compounds were prepared using a \"centroid-simplex\" design and applied to wheat flour for breadmaking. The enzyme showed synergism with ascorbic acid in the dough elasticity suggesting a biochemical interrelation between the two compounds in strengthening the gluten protein matrix. The same synergy between soy lipoxygenase and ascorbic acid was observed with the yellow hue reduction of breads measured instrumentally. From these results, the enzyme action was evaluated with wheat strength and proofing time variations reproducing real processing conditions. This time, a Box-Behnken experimental design with three factors (enzyme activity, wheat strength and proofing time), and three variation levels were applied in the sample preparation. The results showed that soy lipoxygenase (type 1) is a fast-acting oxidizing enzyme and its bleaching effect on wheat flour carotenoid exhibited a positive interaction with wheat strength and proofing time. Rheological and sensory results attributed to the combined action of the different oxidants and breadmaking conditions were for the first time described through polynomial models with predictive capacity.

Compte tenu que la capacité de panification de l'amidon aigre, amidon de manioc modifié par fermentation et irradiation UV, n'a pas encore été complètement élucidée, l'influence du génotype et les traitements post-récolte sur le pouvoir de panification de l'amidon aigre a été l'objectif de ce travail. 13 génotypes de manioc de Colombie, cultivés à 1000 m. et 1700 m.p.n.m (3 de plaine et 10 d'altitude, respectivement) modifiés par fermentation (0 ou30 jours) et séchage (four ou soleil) ont été étudiés. Les analyses de la taille des granules, la viscosité d'empesage RVA et la viscosité intrinsèque révèlent que les traitements post-récolte ont été les facteurs prépondérants en vue de l'amélioration de la capacité de panification tandis que le facteur génotype a présenté une influence moins importante. Parmi les traitements post-récolte, la fermentation a présenté un effet plus prononcé sur la structure des granules d'amidon, que le séchage au soleil. Cependant, la combinaison de ces deux traitements a été nécessaire pour obtenir une forte capacité de panification. L'effet d'altitude de culture a été mis en évidence lors de la fermentation par des sensibilités différentes trouvées entre les granules de plaine et d'altitude (exocorrosion et endocorrosion, respectivement). Les génotypes d'altitude, donc, ont montré une bonne capacité de panification. Néanmoins, d'autres facteurs entrent en jeu, la teneur en amylose mesurée par DSC a influencé négativement l'expansion de la pâte, probablement en raison de la formation de complexes amylose-lipide. Par conséquent, la combinaison de génotypes de manioc (la teneur en amylose principalement) et les traitements post-récolte sont un élément clé de la capacité de panification de l'amidon aigre. Ces contributions peuvent aider à l'établissement de standards de qualité de l'amidon aigre vis à vis de leur potentielle utilisation dans la fabrication des produits sans-gluten. / The breadmaking ability of sour cassava starch, cassava starch modified by fermentation and UV irradiation, have not been yet fully elucidated. The influence of genotype and postharvest treatments on expansion ability of sour cassava was the objective of this work. Thirteen cassava genotypes have been studied. All genotypes proceeded from Colombia. Two altitudes of origin (1000 m. and 1700 m.a.s.l. refered to respectively as lowland and highland), fermentation treatments (0 or 30 days) and drying treatments (oven or sun) were considered. Analyses of the granule size, RVA pasting behavior and intrinsic viscosity were analysed as indicators of breadmaking ability. Results showed that post-harvest treatments were prevailing factors in improving breadmaking ability while the genotype factors had a smaller influence. Among post-harvest treatments, fermentation had a greater influence than sun-drying on these indicators, in particular on starch granular structure. The combination of both treatments was needed to obtain a high capacity bread. Furthermore, the altitude of origin of cassava genotypes was observed to affect the fermentation process: different sensitivities to fermentation were found between granules of lowland and highland (exocorrosion and endocorrosion, respectively). Highland genotypes showed a better breadmaking ability. Other influencing factor related to genotype was the amylose content measured by DSC: it negatively influenced the expansion of the dough, probably due to the formation of amylose-lipid complex. As a conclusion, the combination of cassava genotypes (mainly amylose content) and post-harvest treatment is key for expansion ability of sour starch. This knowledge is a first, essential step to provide quality standards for sour starch, targeted particularly for use in gluten-free products.

Orientador: Caroline Joy Steel / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos / Made available in DSpace on 2018-08-24T06:50:38Z (GMT). No. of bitstreams: 1 Marangoni_AndreLuis_D.pdf: 1214013 bytes, checksum: a252edf1c913d6c97e6f9530017bb9cd (MD5) Previous issue date: 2014 / Resumo: A interesterificação é uma ferramenta fundamental para o desenvolvimento de gorduras "zero trans"; entretanto, comparada ao processo de hidrogenação, esta apresenta limitações, sobretudo no desenvolvimento de gorduras para uso em panificação. De acordo com a literatura, na produção de pão de forma, a gordura exerce diversas funções, como a lubrificação e o aumento da extensibilidade da massa, e o aumento do volume e do sabor do pão. A gordura afeta a textura, mantendo os pães macios por mais tempo; isto se deve possivelmente à sua interação com o amido da farinha, retardando o processo de retrogradação e, assim, estendendo a vida de prateleira do pão. O objetivo deste trabalho foi aplicar a tecnologia de Redes Neurais Artificiais (RNA) na formulação de gorduras "zero trans" à base de óleo de soja e gorduras interesterificadas de soja para facilitar o processo de formulação por blending, específicas para produtos de panificação, e determinar a influência das mesmas na qualidade dos pães de forma e nas interações entre as gorduras e o amido da farinha. Para tanto, foram produzidos pré-misturas e pães de forma com a adição de 4% de gordura. Como padrões, foram utilizadas gorduras comerciais, hidrogenada (GHS) e low trans (GLT), além de óleo de soja (OLS). Também foram utilizados os blends de gordura formulados através da RNA (BL1, BL2, BL3 e BL4). Para efeito de controle, foi produzido um pão sem adição de gordura (C). A análise farinográfica mostrou que a absorção de água (ABS) da farinha de trigo pura (59,0%) foi em média 6,5% maior que a das pré-misturas adicionadas de gordura. O tempo de desenvolvimento (Td) foi menor para as amostras GHS, GLT e BL4. A extensografia mostrou que, dentre todas as amostras, a BL4 foi a mais resistente (980 UE) e a menos extensível (114 mm). Isto provavelmente ocorreu devido ao menor teor de óleo de soja em sua constituição (54%), o que pode ter contribuído para uma massa de maior consistência. A análise dos pães produzidos revelou que apenas os volumes específicos das amostras OLS (3,46 mL/g) e BL4 (4,07 mL/g) diferiram significativamente entre si. A análise de firmeza dos pães mostrou que ao longo da estocagem houve diferença significativa entre a firmeza dos pães com gordura e a amostra controle (1005,75 gf), sendo este valor 13% superior ao da amostra GHS - a mais firme dentre os pães com adição de gordura. A uniformidade do miolo foi maior com a utilização de gordura. Nos pães controle (C), a porosidade (26,73%) foi quase 3 vezes superior ao das amostras com a adição dos blends. Os miolos dos pães BL1, BL2, BL3 e BL4 apresentaram alvéolos pequenos e espalhados mais uniformemente, quando comparados aos pães C, GHS, GLT e OLS. Quanto à umidade, os pães com gordura apresentaram um menor teor em relação ao da amostra controle (35%), pois as suas massas absorveram menos água durante a mistura. A análise térmica através de DSC sugeriu um efeito da gordura sobre o envelhecimento dos pães, uma vez que as variações de entalpia de retrogradação foram menores para os pães com gorduras. Os blends de gordura desenvolvidos usando a RNA e aqui utilizados, além do baixo teor de ácidos graxos trans (1,18% em média), apresentaram-se viáveis para aplicação em panificação, sobretudo o BL4 / Abstract: Interesterification is a fundamental tool in the development of "zero trans" fats; however, when compared to the hydrogenation process, it presents limitations, especially when developing shortenings for bakery products. According to literature, in the production of pan bread, fat has several functions, such as lubrication and an increase in dough extensibility, and an increase in bread volume and flavor. Fat affects texture, maintaining breads soft for a longer period of time; this is possibly due to its interaction with starch in flour, retarding the retrogradation process and, thus, extending bread shelf-life. The aim of this study was to apply Artificial Neural Network (ANN) technology in the formulation of "zero trans" fats based on soybean oil and soybean interesterified fats to ease the formulation process through blending, for use in bakery products, and determine their influence on the quality of pan bread and on their interaction with starch in flour. For this, pre-mixes and breads with the addition of 4% fat were produced. As standards, commercial fats (hydrogenated soybean fat ¿ GHS and low trans fat ¿ GLT) were used, as well as soybean oil (OLS). The fat blends formulated using the ANN (BL1, BL2, BL3 e BL4) were also used. As control (C), bread without fat addition was prepared. The farinographic analysis showed that water absorption (ABS) of pure wheat flour (59.0%) was in average 6.5% higher than that of the pre-mixes of flour and fats. Dough development time (Td) was lower for the samples GHS, GLT and BL4. The extensographic analysis showed that, amongst all samples, BL4 showed the highest resistance to extension (980 EU) and the lowest extensibility (114 mm). This probably occurred due to the lower soybean oil content in its constitution (54%) that could have contributed to a more consistent dough. The analysis of the breads produced revealed that only the specific volumes of the samples OLS (3.46 mL/g) and BL4 (4.07 mL/g) differed significantly. Firmness analysis of breads showed that throughout the storage period studied there was a significant difference between the firmness of the breads with fats and the control sample (1005.75 gf), being this value 13% higher than that of GHS ¿ the firmest amongst samples with fat. Crumb uniformity was greater with the use of fat. In the control breads (C), porosity (26.73%) was almost 3 times greater than that of the samples with the addition of the blends. The crumbs of breads BL1, BL2, BL3 and BL4 presented small and more uniformly distributed alveoli, when compared to breads C, GHS, GLT and OLS. As to moisture content, breads with fat presented lower values when compared to the control sample (35%), as their doughs absorbed less water during mixing. Thermal analysis through DSC suggested an effect of fat on bread staling, once retrogradation enthalpy changes were lower for breads with fats. The fat blends developed using the ANN and used in this study, as well as having a low trans fatty acid content (1.18% in average), showed feasibility for application in pan bread, especially BL4 / Doutorado / Tecnologia de Alimentos / Doutor em Tecnologia de Alimentos

Master of Science / Department of Grain Science and Industry / Jon Faubion / Whole wheat flour has become a popular ingredient in baked goods. Consumers are seeking the added benefits of consuming healthy phytochemicals and bioactive compounds. As the demand for wholemeal flour increases it is important to understand the factors contributing to changes in whole wheat flour breadmaking quality as a function of both wheat and flour age. In wholemeal flour, the lipid composition appears to be a factor causing variable baking quality. Changes that occur in both freshly harvested wheat and milled flour can cause variations in baking quality. Bakers’ attempts to adjust formulations and processes are often unsuccessful. The objective of this study was to determine the effects of aging both new crop wheat and freshly milled flour at 2 different temperatures on baking quality and glycolipid composition. This study aged freshly harvested Overley hard red winter wheat at RT (23˚C) and FZ (-26˚C) for 8, 50, and 91 days before milling. Whole wheat flour baking and lipid extraction studies were performed incrementally over 31 days of flour storage after each milling. Glycolipid structure and amounts were measured using automated electrospray ionization-tandem mass spectrometry. The glycolipids analyzed in this study were DGDG (digalactosyldiglycerol), MGDG (monogalactosylmonoglycerol), MGMG (monogalactosylmonoglycerol), and DGMG (digalactosylmonoglycerol). Both the wheat and flour stored at -26˚C produced larger mean loaf volumes than did the 23˚C treatment for all 3 wheat ages. The FZ and RT DGDG:MGDG ratios were similar over both wheat and flour age so changes to the glycolipid fraction did not appear to be the cause of the significant difference between volumes of bread baked from samples stored at RT and FZ. There was limited change in mean volume over flour age except for an increase at the FZ 91 day point. The DGDG:MGDG ratios were generally unchanged over flour age. Loaf volumes were the same over wheat age except for an unexpected drop during the 50 day wheat study. The corresponding DGDG:MGDG ratios did not show any significant differences over wheat age. The FZ storage temperature might be inhibiting some biochemical change not affecting the glycolipids, leading to higher volumes than the RT storage treatment.

<p>In order to study the <i>effect of production systems</i> aimed at sustainability<i> on product quality</i> and of sensory and non-sensory factors on product acceptance – the effect of farming system, year, milling and baking techniques on the sensory qualities of wheat bread as a model product was investigated using a descriptive test, and the effect of information and values on liking of bread using consumer tests.</p><p>Whole wheat and white breads were baked with wheat grown in six lots in established conventional and organic farming systems in field trails, in two subsequent years for the white bread.</p><p><i>Milling technique</i> influenced flours’ rheology and had greater impact on the sensory qualities of whole wheat bread and on the slice area than did <i>farming system</i> and <i>baking technique</i>. Bread baked with roller-milled wheat was characterized by sweetness, juiciness, compactness and smaller slice area than bread baked with stone-milled wheat, which was characterized by saltiness, deformity and roasted cereals.</p><p>The <i>effect of year </i>on the white bread was greater than the effect of <i>farming system </i>or <i>recipe modification</i>. Bread baked with wheat harvested in 1999 had significantly lower intensities of crumb attributes such as smoothness, juiciness and elasticity, but higher rancid flavour, springiness, compressibility, mastication resistance than bread baked with wheat harvested in 2000. Bread baked with conventional flour had significantly higher juiciness and elasticity than organic bread.</p><p>Image analysis did not show differences in slice area between bread baked with conventionally and organically grown wheat harvested in 1999 compared with 2000. <i>Information</i> affected liking in relation to the type of provided information. Information on organic origin enhanced most liking of bread, particularly for the less liked samples and frequent consumers of organic food. Significantly different <i>values</i> and different specific liking of breads were found among consumer segments. Results linked values and age with “taste”.</p>

In order to study the effect of production systems aimed at sustainability on product quality and of sensory and non-sensory factors on product acceptance – the effect of farming system, year, milling and baking techniques on the sensory qualities of wheat bread as a model product was investigated using a descriptive test, and the effect of information and values on liking of bread using consumer tests. Whole wheat and white breads were baked with wheat grown in six lots in established conventional and organic farming systems in field trails, in two subsequent years for the white bread. Milling technique influenced flours’ rheology and had greater impact on the sensory qualities of whole wheat bread and on the slice area than did farming system and baking technique. Bread baked with roller-milled wheat was characterized by sweetness, juiciness, compactness and smaller slice area than bread baked with stone-milled wheat, which was characterized by saltiness, deformity and roasted cereals. The effect of year on the white bread was greater than the effect of farming system or recipe modification. Bread baked with wheat harvested in 1999 had significantly lower intensities of crumb attributes such as smoothness, juiciness and elasticity, but higher rancid flavour, springiness, compressibility, mastication resistance than bread baked with wheat harvested in 2000. Bread baked with conventional flour had significantly higher juiciness and elasticity than organic bread. Image analysis did not show differences in slice area between bread baked with conventionally and organically grown wheat harvested in 1999 compared with 2000. Information affected liking in relation to the type of provided information. Information on organic origin enhanced most liking of bread, particularly for the less liked samples and frequent consumers of organic food. Significantly different values and different specific liking of breads were found among consumer segments. Results linked values and age with “taste”.

Arabinoxylans (AX’s) are the predominant non-starch polysaccharides found in the structural matrix of cell walls in wheat grains, being present in large quantities in wheat bran, accounting for up to 25% of its composition. Their physicochemical properties define their functionality which can be beneficial in cereal-based products such as bread, where their addition could enhance the gluten matrix responsible for the aerated structure and quality of bread. Bioethanol production has grown rapidly, however, to be economically viable, cereal-derived first generation biorefineries need to adopt the process integration approaches employed in petrochemical refineries, and exploit the interaction opportunities arising from multiple product streams. A potential source of AX is its extraction from the wheat bran based low value animal feed produced as an end product of the bioethanol distillation process. The benefits of extraction are twofold, to enhance feed nutritional value by reducing fibre content and produce a high value product for use as a functional ingredient in the breadmaking industry. Extraction of AX involves precipitation with ethanol, giving opportunity for integration and economic extraction in the context of a bioethanol plant. Currently no commercial supply of AX is available in sufficient quantities to conduct functionality trials; therefore the objective of the current work was to study the feasibility of extracting AX from animal feed and, by developing a scaled-up extraction process based on that of Hollmann and Lindhauer (2005) and Du et al. (2009), to produce sufficient quantities to conduct functionality trials into its effects on the breadmaking process. Two animal feeds, representative of contrasting biorefinery operations, were used for AX extraction, Distillers’ Dried Grains with Solubles (DDGS) and C*Traffordgold®, with water and alkaline AX being extracted from each. Monosaccharide analysis of the feedstocks confirmed the presence of arabinoxylans, with DDGS containing 12.5% AX and TG 13% AX, with A/X ratios of 0.55 and 0.61, respectively. The purity of AX extracted at both lab scale and on scale up was consistent, with 29.5% and 23% extracted at lab scale and 30% and 25% extracted in the scaled up process for WEAX from DDGS and TG, respectively. The purity of AEAX was lower, with 18% and 14% at lab scale and 15% and 14% for scale up for DDGS and TG, respectively. The results indicate that the same purity of crude extract can be achieved at both lab and larger scale extraction, however lower yields and absolute yields were observed at scale up and anticipated to be due to the crude nature of the process, which needs further optimisation. Addition of crude AX extracts to bread dough gave insights into its effects and potential benefits and issues. The type and origin of AX was found to affect bread dough characteristics in different ways. WEAX from both DDGS and TG exhibited effects on all stages of the breadmaking process, by improving stability and reducing softening at mixing, and slowing proving allowing the dough to retain a better aerated structure, leading to an open aerated finished loaf structure. AEAX from both types of animal feed generally caused destabilization of gas cells and coalescence, resulting in loaves with a tighter crumb structure. The AX dosage appeared to have a non-linear effect, with some positive changes at 1% addition turning to negative changes when added at 2%. This work has provided insights into the functionality of AX in bread dough, and a process has been developed to allow greater than lab scale production of arabinoxylan. The effects of AX need to be understood more clearly to retain beneficial effects, whilst supressing detrimental ones, preferably while avoiding the need for extensive purification. The work supports the possibility for AX extracts to be produced commercially as bread ingredients that could enhance bread structure and nutritional quality, and provides an elegant solution for synergy between two wheat-based industries serving both the food and non-food needs of society.