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In-vitro- und In-vivo-Studien zeigen konservierte Eigenschaften des RNAi-Mechanismus' in Trypanosoma bruceiBest, Alexander. January 2005 (has links)
Darmstadt, Techn. Universiẗat, Diss., 2005. / Dateien im PDF-Format.
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VSG-GPI-Ankerfragmente aus Trypanosoma brucei eine neuartige Synthesestrategie /Dettmann, Ralf Peter. January 2002 (has links) (PDF)
Köln, Universiẗat, Diss., 2001.
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Molekulargenetische Untersuchungen zur Differenzierung von Trypanosoma bruceiSchulte zu Sodingen, Cordula. January 2000 (has links) (PDF)
Konstanz, Universiẗat, Diss., 2000.
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Crystallographic investigations of phosphoglycerate kinase from the causative agent of sleeping sickness /Bernstein, Bradley E. January 1997 (has links)
Thesis (Ph. D.)--University of Washington, 1997. / Vita. Includes bibliographical references (leaves [152]-161).
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TbeIF2K2, uma nova quinase de eIF2alfa associada a membrana da bolsa flagelar do trypanosoma brucei / A movel membrane-bound eIF2alfa kinase in the flagellar pocket of Trypanosoma bruceiMoraes, Maria Carolina Strano [UNIFESP] January 2007 (has links) (PDF)
Made available in DSpace on 2015-12-06T23:47:07Z (GMT). No. of bitstreams: 0
Previous issue date: 2007 / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O controle traducional mediado pela fosforilacao da subunidade alfa do fator de inicio de traducao 2 (eIF2a) e um ponto central para programas de expressao genica induzidos por estresse. Tripanossomatideos, importantes patogenos humanos, apresentam processos de diferenciacao desencadeados pelo contato com os distintos ambientes encontrados em seus insetos vetores e hospedeiros mamiferos, provavelmente representando situacoes de estresse. Trypanosoma brucei, o agente causador da tripanossomiase africana, codifica tres potenciais quinases de eIF2α (TbeIF2Kl-K3). Neste trabalho, nos mostramos que TbeIF2K2 e uma glicoproteina associada a membrana, expressa tanto na forma prociclica quanta na sanguinea. 0 dominio catalitico de TbeIF2K2 fosforila eIF2α de levedura e de mamiferos na Ser51. A quinase tambem fosforila a incomum forma de eIF2α encontrada em tripanossomatideos, especificamente no residuo Thr169, que corresponde a Ser51 em outros eucariotos. 0 eIF2α de T brucei, no entanto, nao e um substrato para GCN2 ou PKR in vitro. 0 dominio regulatorio putativo de TbeIF2K2 nao apresenta nenhuma similaridade de sequencia com as quinases de eIF2α conhecidas. Tanto na forma sanguinea quanto na prociclica, TbeIF2K2 esta localizada principalmente na bolsa flagelar, organela que e o local exclusivo de exo e endocitose nesses parasitas. Ela tambem pode ser detectada em compartimentos endociticos, mas nao em lisossomos, sugerindo que a quinase e reciclada entre os endossomos e a bolsa flagelar. A localizacao de TbeIF2K2 sugere que ela possa funcionar como um sensor do transporte de nutrientes ou proteinas em T brucei, um organismo que depende de mecanismos regulatorios pos-transcricionais para controlar a expressao genica em diferentes situacoes. Essa e a primeira quinase de eIF2α associada a membrana descrita em eucariotos unicelulares / Translational control mediated by phosphorylation of the alpha subunit of the eukaryotic
initiation factor 2 (eIF2α) is central to stress-induced programs of gene expression.
Trypanosomatids, important human pathogens, display differentiation processes elicited by
contact with the distinct physiological milieu found in their insect vectors and mammalian
hosts, likely representing stress situations. Trypanosoma brucei, the agent of African
trypanosomiasis, encodes three potential eIF2α kinases (TbeIF2K1-K3). We show here that
TbeIF2K2 is a transmembrane glycoprotein expressed both in procyclic and bloodstream
forms. The catalytic domain of TbeIF2K2 phosphorylates yeast and mammalian eIF2α at
Ser51. It also phosphorylates the highly unusual form of eIF2α found in trypanosomatids
specifically at residue Thr169, that corresponds to Ser51 in other eukaryotes. T. brucei
eIF2α, however, is not a substrate for GCN2 or PKR in vitro. The putative regulatory
domain of TbeIF2K2 does not share any sequence similarity with known eIF2α kinases. In
both procyclic and bloodstream forms TbeIF2K2 is mainly localized in the membrane of
the flagellar pocket, an organelle that is the exclusive site of exo- and endocytosis in these
parasites. It can also be detected in endocytic compartments but not in lysosomes,
suggesting it is recycled between endosomes and the flagellar pocket. TbeIF2K2 location
suggests a relevance in sensing protein or nutrient transport in T. brucei, an organism that
relies heavily on posttranscriptional regulatory mechanisms to control gene expression in
different environmental conditions. This is the first membrane-associated eIF2α kinase
described in unicellular eukaryotes. / FAPESP: 02/13783-9 / BV UNIFESP: Teses e dissertações
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Ordering components of the slender to stumpy signalling pathway in Trypanosoma bruceiMcDonald, Lindsay Mary January 2016 (has links)
In the mammalian bloodstream, the protozoan parasite Trypanosoma brucei undergoes differentiation from proliferative slender forms to arrested, transmissible, stumpy forms. This transition is associated with extensive cytological and metabolic changes that promote survival in the tsetse midgut, and also influences infection dynamics within the mammalian host. A number of genes involved in this transformation were recently identified using an RNAi library screen for resistance to pCPTcAMP, a membrane-permeable cyclic AMP analogue that induces differentiation. These molecules, referred to here as posST (positive mediators of STumpy formation), were thereafter validated to regulate the slender to stumpy transition, with many of them apparently comprising part of a signal transduction and effector pathway. However, it is unknown how these proteins act in relation to one another or are ordered within the pathway. To this end, null mutants were created for several posST components in differentiation-competent pleomorphic trypanosomes and, in this genetic background, other members of the predicted pathway expressed to test their ability to restore stumpy formation. Analysis of distinct combinations has been used to build a preliminary pathway structure model for the signalling events underlying trypanosome quorum sensing. In addition, phosphoproteomic analysis of two null mutants has revealed downstream signalling effects of two posST kinases, MEKK1 and YAK. A similar extragenic suppression approach was also applied to explore the interaction between the identified drivers of stumpy formation and the target of rapamycin kinase, TOR4, which has previously been shown to act as a negative regulator of stumpy formation in monomorphs. Dual ablation of TOR4 and posST components revealed insight into the intersection of stumpy-promoting and stumpy-inhibiting pathways. Finally, a chemical-genetic approach was used to investigate the posST pathway using two differentiation-inducing compounds: the previously studied E667, and GKI7, newly identified from a kinase inhibitor set. RNAi lines for different posST components were tested for their ability to undergo development in the presence of these compounds. An RNAi library screen using GKI7 identified putative new mediators of stumpy formation.
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Characterisation of two protein disulfide isomerases from the endocytic pathway of bloodstream forms trypanosoma bruceiRubotham, Joyce January 2004 (has links)
Doctorat en Sciences / info:eu-repo/semantics/nonPublished
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Nitroheterocycle metabolism in Trypanosoma bruceiJackson, Adrian John Stuart January 1987 (has links)
No description available.
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A study on the membrane skeleton of Trypanosoma bruceiWoods, Angela January 1990 (has links)
No description available.
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Studies on the local skin reaction against African trypanosomesScott, A. J. January 1986 (has links)
No description available.
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