La presente tesis doctoral pretende presentar alternativas al sistema de salado tradicional (salado en pila) utilizando el salado en bolsa como alternativa para controlar la cantidad de sal que se utiliza en el proceso y la salmuera generada. Así mismo, mediante la utilización de las técnicas de espectroscopía de impedancia y voltametría se pretende realizar un control de proceso que probablemente repercuta en la reducción de pérdidas por productos anómalos, optimización del uso de las instalaciones industriales, y la homogeneidad de la calidad del producto final, pudiendo adecuar los lotes a las preferencias del mercado de destino (nivel de sal, grado de curación), Adicionalmente el uso de bolsas en el proceso de salado permitirá mejorar el manejo de la salmuera residual disminuyendo el impacto ambiental. / Girón Hernández, LJ. (2014). Uso de sensores electrónicos para la optimización y control del proceso de elaboración del jamón curado [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/39109 / TESIS

Accelerated global warming plus an increasing need for energy is an equation not easily solved, thus new forms of sustainable energy production are urgently requested. In this context hydrogen production based on a cyanobacterial system offers an environmentally friendly alternative for energy capture and conversion. Cyanobacteria can produce hydrogen gas from sun light and water through the combination of photosystems and hydrogenases, and are suitable to cultivate in large scale. In the present thesis the maturation process of [NiFe]-hydrogenases is investigated with special focus on transcription of the accessory genes encoding proteins needed for assembly of the large and possibly also for the small hydrogenase subunit. The cyanobacteria used are two N2-fixing, filamentous, heterocystous strains; Nostoc sp. strain PCC 7120 and Nostoc punctiforme PCC 73102. For a biotechnological exploration of hydrogen production tools for regulatory purposes are important. The transcription factor CalA (cyanobacterial AbrB like) (Alr0946 in the genome) in Nostoc sp. strain PCC 7120 was found to be involved in hydrogen metabolism by regulating the transcription of the maturation protein HypC. Further the bidirectional hydrogenase activity was down-regulated in the presence of elevated levels of CalA, a result important to take into account when optimizing cyanobacteria for hydrogen production. CalA regulates at least 25 proteins in Nostoc sp. strain PCC 7120 and one of the down-regulated proteins was superoxide dismutase, FeSOD. The characterization of FeSOD shows that it has a specific and important function in the oxidative stress tolerance of Nostoc sp. stain PCC 7120. Since CalA is involved in regulation of both the hydrogen metabolism as well as stress responses these findings indicate that Alr0946 is an important transcription factor in Nostoc sp. strain PCC 7120 active on a global level in the cell. This thesis adds more knowledge concerning maturation and regulation of cyanobacterial hydrogenases which might be useful for future large scale hydrogen.

With accellerating global warming and pollution problems a change of energy regime is necessary. Solar energy offers a clean and unlimited energy source of enormous potential. Due to it’s intermittenet nature solar energy must be stored - ideally in the chemical bond of a carrier molecule. Hydrogen gas, H2, an energy carrier with water as only emission when used in a fuel cell, is considered to be the choise for the future. In this context cyanobacteria show promising potential as future H2 factories since they can produce H2 from solar energy and water. The main enzymes directly involved in cyanobacterial hydrogen metabolism are nitrogenases and hydrogenases. Cyanobacterial hydrogenases are either uptake hydrogenases or bidirectional hydrogenases and their maturation requires assistance of six maturation proteins and two hydrogenase specific proteases. In this thesis the transcriptional regulation, maturation and function of the cyanobacterial uptake hydrogenases were investigated in the filamentous, heterocyst forming strains Nostoc punctiforme ATCC 29133 and Nostoc sp. strain PCC 7120. Five genes, encoding proteins putatively involved in the maturation of the uptake hydrogenase were identified upstream the known maturation genes. Two transcription factors, CalA and CalB, were found interacting with the stretch of DNA forming the upstream regions of the uptake hydrogenase structural genes and the novel maturation genes. The expression of the uptake hydrogenase were  heterocysts specific and the specificity mapped to a short promoter region starting -57 bp upstream the transcription start point. In addition, the function of the uptake hydrogenase was inserted in a metabolic context. Among the proteases, a conserved region was discovered possibly involved in determining the hydrogenase specificity. This thesis has given valuable information about the transcriptional regulation, maturation and function of the uptake hydrogenase in filamentous, heterocystous cyanobacteria and identified new targets for bioengineering of mutant strains with higher H2 production rates.

In times with elevated CO2 levels and global warming there is a need of finding alternatives to carbon based energy carriers. One such environmental friendly solution could be H2 produced by living organisms. Cyanobacteria are good candidates since they can produce H2 from sunlight and water through the combination of photosynthesis and H2 producing enzymes i.e. nitrogenases and/or [NiFe]-hydrogenases. This thesis investigates the maturation and transcriptional regulation of [NiFe]-hydrogenases in cyanobacteria, with a special focus on hydrogenase specific proteases. The core of all hydrogenases consists of the small and large subunit. The large subunit in which the catalytic site is located goes through an extenstive maturation process which ends with a proteolytic cleavage performed by a hydrogenase specific protease (HupW/HoxW). This thesis shows that within the maturation process of hydrogenases, the proteolytic cleavage is probably the only step that is specific with respect to different types of hydrogenases i.e. one type of protease cleaves only one type of hydrogenase. Further in-silico analysis revealed that these proteases and the hydrogenases might have co-evolved since ancient time and that the specificity observed could be the result of a conserved amino acid sequence which differs between the two types of proteases (HupW/HoxW). A number of different transcription factors were revealed and shown to interact with the promoter regions of several of the genes encoding maturation proteins. The results indicate that the hydrogenase specific proteases are regulated on a transcriptional level in a similar manner as the hydrogenases they cleave. This thesis contributes with knowledge concerning transcriptional regulation and protein regulation of hydrogenases which will be useful for designing genetically engineered cyanobacteria with an improved and adjustable H2 production. / Felaktigt tryckt som Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 722

The diploma thesis is focused on the research of dynamics of selected heating surfaces behavior. The aim of the thesis is to determine the dynamics of heating and cooling and to determine the effect of these characteristics on energy consumption of the building. The project part deals with the design of a heating solution for a residential building in three variants. An Energetic simulation is made for the designed variants, that compares the consumption of thermal energy during one year. The next simulation research the dynamics of selected large-scale heating surfaces. For each construction, nonstationary models of heating up and cooling were made, which are compared in terms of the thermal inertia.

This thesis deals with the optimization of sewer heat recovery. For proper design and optimization it is necessary to use three basic methods: diagnostics, heat balance calculations and modeling. In 2D software Area Calculation are simulated different types of sewer exchangers arranged in sewer pipes. Using models of software CalA and simulations will be selected optimal variant. Using software BSim, which is based on more zone numerical simulation of the energy needs of buildings in relation to indoor climate, are detected heat needs of an office building. The optimal number of sewer exchanger is designed from the identified performance heat exchangers and supplies heat for the building.

The PhD thesis deals with the theoretical evaluation of the potential use of night cooling techniques in condition of the Czech Republic. Mainly aims to evaluate the potential of night cooling, which uses the physical phenomenon of radiation against the night sky. This technique is widely used mainly in the dry and hot regions where there are the most suitable climatic conditions for this cooling method. The work is based on the current state of knowledge in the area of passive and low-energy cooling systems. These knowledges are applied to the conditions of Czech Republic and by using simulations of the energy, behaviour of the buildings explores the benefits in terms of improving of the internal microclimate and from the perspective of economy operation of conventional cooling equipment. In order to establish the benefits of night cooling on the operation of the building in the dynamically changing conditions, the modular simulation program TRNSYS was used, which is suitable for the analysis of energy systems of buildings and their behaviour. The results of simulation results were converted to thermal comfort parameters of the PMV and the PPD.

This item contains the agenda, minutes, and attachments for the Faculty Senate meeting on this date. There may be additional materials from the meeting available at the Faculty Center.

The master’s thesis deals with optimization of cooling system of family house using borehole heat exchanger as a single cooling source. In the experimental part of the thesis was carried out long-term mearurements. The main part of the master’s thesis is the development and verification mathematical and physical model of thermal behavior chilled room. The result are computer simulations and evaluation of individual scenarios and design requirements for a potential optimization of measurement and control system. The theoretical part generally deals with radiant cooling systems.

El análisis de estructuras paleokársticas ha atraído, en los últimos años, el interés de numerosos investigadores a la información que aportan a la geología aplicada y la paleogeomorfología. Estudios recientes se han centrado en la aplicación de técnicas de exploración del subsuelo debido a la escasez de afloramientos. En la presente Memoria se analizan íntegramente las formas de hundimiento pretéritas que afloran discontinuamente con gran detalle, en los acantilados de las costas meridional (plataforma de Llucmajor) y oriental (plataforma de Santanyí) de Mallorca, a lo largo de más de 75 km de línea de costa, afectando a las rocas carbonáticas del Mioceno superior. El estudio se ha centrado en la distribución geográfica, evolución geológica y las características geomorfológicas de estos paleocolapsos, con especial énfasis en su génesis, su relación con la arquitectura y distribución de las facies, así como en las formas y productos asociados.Los paleocolapsos han sido descritos en su contexto litoestratigráfico y estructural dentro de las mencionadas plataformas carbonáticas, siendo este trabajo una contribución al conocimiento del karst en estas unidades geológicas y su relación con las fluctuaciones marinas. La karstogénesis queda reflejada en estas formas pretéritas donde se han observado depósitos y formas de disolución ligadas a la dinámica kárstica controlada, en el caso que nos ocupa, por las fluctuaciones del nivel del mar: brechas, sedimentos detríticos, cementos, así como distintos tipos y volúmenes de porosidad. La mayor parte de estas formas (sobre un total de 177), cuyas dimensiones en sección varían desde pocos metros hasta afloramientos con 28 m de altura y más de 100 m en la horizontal, se ubican en la plataforma de Santanyí a excepción de dos estructuras ubicadas en la plataforma de Llucmajor.El análisis geológico y su relación con los paleocolapsos muestra como en la plataforma de Llucmajor éstos afectan a las facies de la Unidad Complejo Arrecifal (facies de back reef y frente arrecifal). Sin embargo, en la plataforma de Santanyí, los paleocolapsos afectan tanto a parte del Complejo Arrecifal (facies de back reef), como a la totalidad de la Unidad Calizas de Santanyí. A partir del estudio de la arquitectura de facies del Complejo Arrecifal en la plataforma de Llucmajor se ha establecido el modelo deposicional en la plataforma de Santanyí. Sin embargo, ésta última se encuentra compartimentada como consecuencia del control de dos fallas en dirección de orientación E-O en S'Algar y Na Magrana, donde se localiza el contacto entre facies de lagoon externo y talud arrecifal. No obstante, la cartografía y análisis de los lineamientos en dicha plataforma ha permitido identificar dos familias principales con dos direcciones dominantes; NE-SO y NO-SE, siendo la dirección E-O menos representativa. Se han observado fracturas distensivas y pequeñas fallas inversas miocenas asociadas al proceso de colapso, así como fracturas y fallas postmiocenas, y fracturas cuaternarias.El estudio de la geometría en sección de los paleocolapsos pone de relieve que la formas en "V", "U" y conoidales son las más comunes. Han sido identificadas dos partes diferentes en un paleocolapso tipo: una inferior donde se observa la paleocavidad ubicada en la base del paleocolapso (lagoon externo y/o frente arrecifal), con una geometría irregular de dimensiones entre 1 m y 9 m rellena por sedimentos adyacentes y suprayacentes a ésta; y una parte superior, coincidente con los bordes de la estructura (lagoon interno/Calizas de Santanyí) buzando con inflexión conoidal hacia la paleocavidad.Se han identificado cuatro tipos de brechas (crackle, crackle-laminae-split, de mosaico y caótica) en las estructuras de paleocolapso asociadas cada una de ellas a distintos niveles estratigráficos y, en algunos paleocolapsos, con una gradación vertical y lateral. Son característicos de estos depósitos los sedimentos detríticos (matriz) y los cementos asociados (vadosos y freáticos). En general, el cemento domina sobre la matriz en la zona inferior del paleocolapso, mientras que por encima, es la matriz la que domina sobre el cemento. El análisis por difracción de Rayos X de la matriz indica para la muestra total que la calcita es el mineral principal y el cuarzo el mineral secundario. En la fracción arcilla, la moscovita, la illita y la caolinita son los minerales más comunes. De ello, junto con el estudio de láminas delgadas en estos depósitos, donde se han observado tamaños de grano en el cuarzo superior a 2 mm, se deduce un ambiente de sedimentación subsuperfical y otro subaéreo de lo que se extrae un origen, proceso de transporte y sedimentación diversos, así como la evolución cristaloquímica en determinados minerales. Los cementos son de naturaleza calcítica, con contenidos relativamente altos en magnesio para los freáticos y bajos para los vadosos. Para el estudio de la porosidad en los paleocolapsos se ha procedido a su clasificación en dos tipos principales, interclasto e intraclasto, a partir de las cuales se ha estudiado la macro y microporosidad. La brecha caótica de colapso es la que presenta volúmenes de porosidad más elevados y tipologías diversas. El análisis de isótopos estables muestra una gran homogeneidad entre la composición isotópica de los cementos, con valores en δ18O y δ13C ligeros, lo que indica condiciones análogas de precipitación, con dominio de aguas dulces sobre las saladas. Tanto la marca del oxígeno como del carbono parecen indicar que los cementos se depositaron en un período interglaciar coincidente con algún estadio isotópico impar.El estudio de la arquitectura de facies de la plataforma de Llucmajor ha permitido elaborar un modelo genético de ocurrencia para los paleocolapsos y su ubicación espacio-temporal. Dicho modelo, ha sido corroborado por la relación entre la distribución de facies y paleocolapsos en la plataforma de Santanyí, por la observación en algunos paleocolapsos de sedimentos a techo de la Unidad Calizas de Santanyí que sellan la estructura, así como por el tipo de brechas características de colapsos sinsedimentarios (brecha crackle-laminae-split), que muestran una deformación dúctil de los materiales cuando éstos no estaban completamente consolidados, dando lugar a formas laxas de bajo ángulo. Los procesos genéticos que dieron lugar a los paleocolapsos kársticos están directamente relacionados con la alta frecuencia de fluctuación del nivel del mar durante el Mioceno superior, la misma que controló la arquitectura de facies y la posición del nivel freático. Las oscilaciones del nivel freático causaron la alternancia de dominios freáticos y vadosos así como, de agua dulce y agua salada en la interfase, provocando la disolución de los parches coralinos y el posterior hundimiento del techo de las cavidades. El estudio integral de todos estos aspectos junto con el análisis de una red de paleocauces y una playa fósil, ha permitido realizar una reconstrucción paleogeográfica desde el Messiniense en la plataforma de Santanyí e identificar estructuras de paleocolapso postmiocenas y cuaternarias. Con estos datos se ha procedido a la comparación de los paleocolapsos kársticos con otras estructuras similares en el País Vasco y Las Islas de Malta, de lo que se extraen analogías y diferencias, determinadas fundamentalmente por el orden de fluctuación del nivel del mar. Por último, se discute el papel de los paleocolapsos kársticos como elementos que contribuyen en cierta medida a la ocurrencia de hidrocarburos en plataformas carbonáticas, pudiendo ser excelentes reservorios debido al gran número de afloramientos, el volumen de roca afectada y a su elevada porosidad y permeabilidad. / Paleokarst tend to differ from studies of recent and modern karst landforms though is important the genetic understanding of the karst processes for analysis a paleokarst structure. Paleokarst systems form an important class of carbonate record and they have a pronounced lateral and vertical spatial complexity that results from a complex history of formation. Most of the known karst systems are epigenetic and they are the result of near-surface karst processes during periods of subaerial exposure and latter burial compaction and diagénesis. Scale, porosity types and spatial complexities of these paleokarst systems depends on the carbonate rock solubility, paleoclimatic conditions, lowering of base level either by tectonic uplift or sea-level fall and time of subaerial exposure. Uplift, in addition, commonly induces fracturing and faulting that further control karst development. Ascertaining and predicting paleokarstic heterogeneities within carbonate rocks are strategic to fluids field development and optimum production. With current subsurface methods, however, most of the smaller-scale stratigraphic architecture and diagenetic facies are difficult to define. Predictive models for exploration and development are best made from outcrop studies of well-exposed examples. Accuracy for prediction of these models depends on the detailed understanding of the genetic factors controlling their geometries, scale, pore networks and spatial complexities of these potential karstic store. Miocene carbonates (Upper Tortonian-Lower Messinian) in Mallorca Island are composed of reefal (Reef Complex) and shallow water carbonates (Santanyí Limestone) that prograded across platforms surrounding paleoislands. The contact between the Reef Complex and the Santanyí Limestone is a subaerial erosion surface with paleokarst features. The shallow-water carbonates beds both the lagoonal beds of the Reef Complex and basal beds of the Santanyí Limestone, are affected by paleocollapse structures produced by roof collapse of caverns developed in the underlying Reefal Complex. These paleocollapse structures affecting to the carbonate platform allows to propose a genetic model to explain the origin of these paleosink, that are related to early diagenetic processes induced by high-frequency sea-level fluctuations, the same sea-level fluctuations that controlled the facies architecture of the carbonate platforms.Cartography and study of lineaments and fractures on Santanyí Platform have permitted identified two principals groups with two main directions: NE-SO and NO-SE. Have been observed distensiva fractures and Miocene small inverse faults related with de breackdwon phenomena. Moreover, postmiocenes and quaternary faults and fractures have been recognized.The geometry of paleocollapse structures is commonly (in section) as "V", "U" or funnel. The size is variable from few meters of long to thousands meters, and few meters of weigh to thirteen meters. Breccias has been classified as crackle, crackle-laminae-split, mosaic and chaotic types. Chaotic breccias grade from matrix-free, clasts-supported breccias to matrix-supported breccias. The matrix mineralogy is compose in the total sample for calcite in the major part and quartz in less quantity. However, same structures present quartz as principal mineral. To the clay fraction, caolinite, illite and moscovite are the most general mineral present.The geochimical sediment (carbonate) are filling a part of interclaste breccias porosity. This is commonly phreatic speleothems. Isotopic studies of this sediments show δ18O and δ13C contents negatives. This fact could indicate a fresh water environment deposition