The key objective of this study was to examine whether leucocyte depletion generated or removed platelet-derived microvesicles in platelet concentrates for transfusion. Three in-process leucocyte removal filters for pooled buffy coat derived platelet concentrates, i. e. negative charged polyester, positively charged polyester, and non-charged polyurethane, were compared. The effects of three major leucocyte depletion technologies currently in use in the UK, i. e. Cobe LRS and Haemonetics MCS+ LD apheresis, and filtration of pooled buffy coat derive platelets, on platelet microvesiculation were also examined. Furthermore, the effects of various leucocyte filters and leucocyte depletion technologies on platelet activation and the activation of coagulation/complement systems were investigated. The procoagulant and anticoagulant properties of microvesicles isolated from platelet concentrates were explored. Leucocyte filtration of pooled bully coat derived platelet concentrates by all three filters did not have a net effect on the level of microvesicles. All three leucocyte depletion technologies gave similar values of microvesicles on day 1, but on day 5 MCS+ LD apheresis showed the lowest value, whilst Cobe LRS apheresis and buffy coat methods were equivalent. Among the three filters, the negatively charged filter activated the coagulation system as measured by kallikrein-like and thrombin-like activities, but removed some activated complement C3a, whereas the positively charged filter generated C3a. Among the three leucocyte depletion technologies, platelets prepared by Cobe LRS showed the lowest degree of activation of the coagulation system. However, both Cobe LRS and MCS apheresis showed higher levels of C3a than filtered buffy coat derived platelets. The microvesicles isolated from day 1 platelet concentrates could act as a catalytic surface for both the coagulant and anticoagulant reactions as measured by the formation of prothrombinase complex and the inactivation of FVa by activated protein C. The microvesicles isolated from day 5 platelets showed an increased procoagulant activity, whereas the anticoagulant activity substantially diminished.

206 leaves : / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (M.D.)--Dept. of Medicine, University of Adelaide, 1974

The purpose of this study was to evaluate functional and funduscopic outcomes amongst patients receiving multiple treatments with macular laser photocoagulation for clinically significant diabetic macular edema. A record review was conducted of patients who had multiple macular laser treatments for diabetic macular edema. As part of routine follow-up for diabetic macular edema, visual acuity and funduscopic findings were assessed before a given laser treatment and at 6 months afterwards. The study included 64 eyes from 41 patients. There was no statistically significant difference between the proportion of eyes that showed funduscopic improvement after treatment 1 versus the proportion of eyes that improved after subsequent treatments. For the first laser treatment, 44 of the 64 eyes (69%) showed funduscopic improvement in edema. 35/64 (55%) of eyes showed improvement after the second treatment (p=0.15); 29/40 (72.5%) eyes showed improvement after the third treatment (p=0.85); 15/18 (83.3%) eyes showed improvement after treatments[greater than or equal to]4 (p=0.36). Similarly, in terms of visual acuity outcomes, there was no statistically significant difference between the proportion of eyes with preserved visual acuities after treatment 1 compared to repeat treatments. This study found that the majority of eyes that receive re-treatment after initial laser therapy will respond with an improvement in macular edema.

Thesis (Ph.D.)--Cleveland State University, 2009. / Abstract. Title from PDF t.p. (viewed on Jan. 13, 2010). Includes bibliographical references (p. 124-131). Available online via the OhioLINK ETD Center and available in print.

Thesis (Ph.D.)--Virginia Commonwealth University, 2009. / Prepared for: Dept. of Medicinal Chemistry. Title from resource description page. Includes bibliographical references.

Background: Blood clot formation is one of the first events in bone regeneration and osseointegration. The blood clot adheres to dental implants with hydrophilic surfaces more favorably than to those with hydrophobic surfaces. This appears to result in better bone healing and bone fill of defects around dental implants. Objective: To assess the impact of blood clot stabilization at modSLA titanium implants on bone formation in chronic-type defects in a dog model. Material & methods: Ten modSLA implants were installed in 5 dogs after creation of saddle-type buccal-lingual bony defects. In 5 implants (test sites), the blood clot was removed by sterile saline irrigation, while the clot was left undisturbed on the other 5 implants (control sites). After 8 weeks of healing, the animals were sacrificed and sections were prepared for histomorphometric analysis. The following measurements were performed: The residual defect length (DL), the buccal and lingual most coronal level of bone in contact with the implant (CBI-b and CPI-l), the new bone height (NBH), the percentage of bone to implant contact (BIC), the area of new bone fill (BF), the difference in buccal and lingual dimensions of CBI (D-CBI), and percentage of linear bone fill (PLF). Results: the mean values of DL were similar in both groups (3.4 mm). All parameters assessed were consistently more favorable in control sites: CBI-b: 1.3 vs. 1.5, CBI-l: 1.3 vs. 0.8, D-CBI: -0.2 vs -0.5, NBH: 1.9 mm vs. 2.1 mm, PLF: 57.1% vs. 64.5% and BF: 4.4 mm? vs. 6.0 mm?. However, these differences were not statistically significant. Conclusion: In the light of consistently more favorable parameters assessed for the healing of saddle-shaped bony defects around implants, it is assumed that a stabilized blood clot contributed to early bone regeneration and osseointegration. Undisturbed blood clot formation may, indeed, be a prerequisite for optimal treatment outcomes. However, owing to the small sample size in the present study, these tendencies ought to be explored in further studies. / published_or_final_version / Dental Surgery / Master / Master of Dental Surgery

No description available.

Thesis (MEng(HydrologyWaterResources))--University of South Australia, 2004.

Thesis (PhDAppliedScience)--University of South Australia, 2003.

Thesis (PhD)--University of South Australia, 2000