Thesis (M.S.)--Duquesne University, 2004. / Title from document title page. Abstract included in electronic submission form. Includes bibliographical references (p. 55-60) and index.

Hydrodynamic slip is known to suppress vorticity production at the solid-fluid boundary in bluff body flows. This suppression combined with the enhanced vorticity convection results in a substantial reduction in the unsteady vortex shedding and the hydrodynamic loads experienced by the bluff body. Here, using combined theoretical and computational techniques, we investigate the effect of slip on three-dimensional wake dynamics and convective scalar transport from a circular cylinder placed in the uniform cross-flow of a Newtonian incompressible fluid over Reynolds numbers ranging from 0.1 to 1000. We find the wake patterns to be strongly influenced by the degree of the slip, quantified through the non-dimensional slip length in the Naiver slip model, with the asymptotic slip lengths of zero and infinity characterizing no-slip and no-shear boundaries, respectively. With increasing slip length, the wake three-dimensionality, that is observed in the case of a no-slip surface for Re > 190, is gradually suppressed and eventually eliminated completely. For each Reynolds number, we identify the critical slip length beyond which the three-dimensionality is completely suppressed and the wake becomes two-dimensional, on the basis of the total transverse entropy present in the flow field. Over the Reynolds number range considered in this work, we find the critical slip length to be an increasing function of Reynolds number. For sufficiently large slip lengths, we observe suppression of two-dimensional vortex shedding leading to formation of a steady separated wake. Further increments in slip length lead to reduction in the intensity and size of the recirculating eddy pair eventually resulting in its complete disappearance for a no-shear surface for which the flow remains attached all along the cylinder boundary. Next, we quantify the effect of hydrodynamic slip on convective transport from an isothermal circular cylinder placed in the uniform cross flow of an incompressible fluid at a lower temperature. For low Reynolds and high P´eclet numbers, theoretical analysis based on Oseen and thermal boundary layer equations allows us to obtain explicit relationships for the dependence of transport rate on the prescribed slip length. We observe that the non-dimensional transport coefficients follow a power law scaling with respect to the P´eclet number, with the scaling exponent increasing gradually from the lower asymptotic limit of 1/3 for the no-slip surface to 1/2 for a no-shear boundary. Results from our simulations at finite Reynolds number indicate that the local time-averaged transport rates for a no-shear surface exceed the one for the no-slip surface all along the cylinder except in the neighbourhood of the rear stagnation region, where flow separation and reversal augment the transport rates substantially.

Orientador: Alexandre Secorun Borges / Resumo: A síndrome da fragilidade cutânea equina, conhecida como warmblood fragile foal syndrome (WFFS), é uma enfermidade do tecido conjuntivo de caráter autossômico recessivo e é caracterizada clinicamente por lesões cutâneas e mucosa da cavidade oral, articulações hiperextensíveis e abortamento. Tais características são incompatíveis com o desenvolvimento dos animais afetados. O polimorfismo de nucleotídeo único (SNP) c.2032G>A no gene PLOD1 (procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1) em homozigose é responsável pela enfermidade em equinos de raças com aptidão para esportes equestres olímpicos. Existe apenas um caso publicado de um potro afetado com a confirmação da mutação, na Suíça, e apenas dois estudos de prevalência do SNP causador da doença, na Alemanha e nos Estados Unidos. Casos descritos com sinais clínicos compatíveis com a enfermidade e descritos antes da mutação ser descoberta estão presentes na literatura. Atualmente as associações de equinos na Europa tem dado grande atenção para esta enfermidade em equinos warmblood. A escassez de estudos e de informações sobre a existência da enfermidade no Brasil e no mundo leva-nos a acreditar que a mesma possa ser subdiagnosticada em nosso e nos outros países. O objetivo desse estudo foi estimar a frequência alélica do SNP c.2032G>A no gene PLOD1 em equinos da raça BH no Brasil, utilizando a reação em cadeia de polimerase e o sequenciamento direto da região do SNP como metodologia diagnóstica. Foram coletadas amostras d... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Warmblood fragile foal syndrome (WFFS), is a connective tissue disease that presents an autosomal recessive character and is clinically characterized by cutaneous lesions, lesions in the gums and mucosa of the oral cavity, hyperextensible joints and abortions. Such characteristics are incompatible with the development of the affected animals. The single nucleotide polymorphism (SNP) c.2032G> A in the PLOD1 gene (procollagen-lysine, 2-oxoglutarate 5- dioxygenase 1) in homozygosis is responsible for the disease. There is only one report, describing an affected foal and the mutation in Switzerland and there are two studies of prevalence of the causative SNP of the disease (one in Germany and the other in the United States). Cases report presenting clinical signs compatible with the disease and described before the mutation was discovered are present in the literature. Currently the equine associations in Europe are giving great attention to this disease in warmblood horses. The scarcity of studies and information about the existence of the disease in Brazil and in the world leads us to believe that it can be underdiagnosed in our and in other countries. The objective of this study was to estimate the allelic frequency of c.2032G>A SNP in the PLOD1 gene in Brazilian Sport Horses in Brazil, using the polymerase chain reaction and the direct sequencing of the SNP region as a diagnostic tool. Blood and hair bulb samples were collected from 374 animals for DNA extraction, subsequent ... (Complete abstract click electronic access below) / Mestre

Branching is a highly plastic trait, enabling plants to adapt their growth form in response to environmental stimuli. In flowering plants, shoot branching is regulated through the activity of axillary buds, which grow into branches. Several classes of plant hormones have been shown to play pivotal roles in regulating bud outgrowth. Auxin derived from the primary shoot apex and active branches inhibits bud outgrowth, whereas cytokinin promotes it. Strigolactones also inhibit bud outgrowth, by changing properties of the auxin transport network, increasing the competition between buds. This occurs by modulating access to the polar auxin transport stream (PATS) in the main stem. The PATS provides directional, long distance transport of auxin down the stem, involving basal localisation of the auxin transporter PIN-FORMED1 (PIN1). Buds need to export their auxin across the stem towards the PATS in order to activate, but since PIN1 is mainly expressed in narrow files of cells associated with the stem vasculature, PIN1 itself it is unlikely to facilitate this connectivity. This thesis re-examines the role of auxin transport in the stem, showing that, besides the PIN1-mediated PATS, other auxin transport proteins constitute a more widespread and less polar auxin transport stream, allowing auxin exchange between the PATS and surrounding tissues. Disruption of this transport stream is shown to reduce bud-bud communication and to partially rescue the increased branching observed in strigolactone mutants. Furthermore, it is shown that distinct classes of auxin transport proteins within this stream can differentially affect bud outgrowth mediated by BRANCHED1 (BRC1). BRC1 is a transcription factor proposed to determine bud activation potential. Taken together, the data presented here provide a more comprehensive understanding of the shoot auxin transport network and its role in shoot branching regulation.

Made available in DSpace on 2014-06-11T19:33:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-07-21Bitstream added on 2014-06-13T20:44:59Z : No. of bitstreams: 1 bonfante_s_dr_araca.pdf: 1718096 bytes, checksum: f842700dfddf9a4f0edab9314172252a (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo do presente estudo foi avaliar histológica e histometricamente o padrão de cicatrização de defeitos de deiscência periodontal tratados com enxerto de tecido conjuntivo subepitelial (ETC) ou matriz dérmica acelular (MDA), cada qual comparado ao tratamento com Retalho posicionado Coronal (RPC). Foram utilizados 10 cães, divididos em dois grupo de 5. Defeitos de deiscência óssea (6x8 mm) foram criados nos caninos superiores e os grupos divididos seguindo um modelo de boca dividida de acordo com o tratamento. Em um grupo de 5 cães foi realizado o tratamento com ETC e no lado contralateral RPC e o outro grupo de 5 cães foi tratado com MDA e RPC no lado contralateral. Após 3 meses pós-operatórios os animais foram submetidos à eutanásia e os blocos processados para análise histológica e histométrica. Os parâmetros histométricos avaliados incluíram extensão de tecido epitelial (TE), nova inserção (NITC) e aposição de tecido conjuntivo (ATC), novo cemento (NC) e novo osso (NO). Os dados histométricos transformados em porcentagem, foram estatisticamente analisados pelo teste ANOVA, seguido pelo teste de Tukey... / The aim of the present study was to evaluate histologically and histometrically the healing pattern of periodontal dehiscence defects treated with Subepithelial Connective Tissue Graft (CTG) or Acellular Dermal Matrix (ADM), when compared to the Coronally Positioned Flap (CPF). Dehiscence bone defects (6x8mm) were created bilaterally in the maxillary canine area of dogs (n=10, divided in 2 groups). By means of a split mouth test design, a group of 5 dogs was randomly allocated to receive subepithelial connective tissue graft (CTG) in one of the sides and a coronally positioned flap (CPF) in the opposing side. The remaining 5 dogs had acellular dermal matrix graft (ADM) as a treatment in either one of the sides and CPF on the other side. Three months after surgery, dogs were euthanized and blocks of interest were processed. The histometric parameters for the latter included lenght of epithelial tissue (ET), new attachment (NACT) and connective tissue aposition (CTA), new cementum (NC), and new bone (NB). Histometric data were converted to percentage and statistically analyzed by ANOVA followed by Tukey test at a p<0.05. No significant difference was detected in the following parameters for both groups: ET yielding... (Complete abstract click electronic access below)

Les muscles se forment au cours du développement embryonnaire, principalement grâce aux capacités de prolifération et différenciation des cellules souches musculaires, néanmoins ces capacités sont insuffisantes pour le développement correct des muscles. La formation des muscles est aussi régulée par des signaux provenant de tissus adjacents, parmi lesquels le tissu conjonctif (TC). Plusieurs facteurs de transcription spécifiquement exprimés dans le TC ont été identifiés comme étant impliqués dans la myogenèse caractérisant ainsi le TC comme une source importante de signaux dans le mécanisme de morphogénèse musculaire. Ces observations soulignent l’importance du rôle du TC dans la formation des muscles, cependant la nature moléculaire des mécanismes médiés par le TC reste à ce jour inconnue. L’objectif de ce travail de thèse a été d’établir le rôle des chimiokines CXCL12 et CXCL14 dans l’intéraction entre le TC et le développement musculaire, en utilisant l’embryon de poulet comme modèle. Dans un premier temps, nous avons défini le patron d’expression de CXCL12 et CXCL14 au cours du développement embryonnaire, et avons mis en évidence une corrélation entre la localisation de ces chimiokines et l’expression de gènes spécifiques à différentes sous populations du TC. Afin d’évaluer le rôle potentiel de ces chimiokines dans la différentiation du TC nous avons utilisé des approches de gain de fonction in vitro et in vivo et avons montré que CXCL12 et CXCL14 activent les facteurs de transcription spécifiques à différentes sous population du TC, démontrant ainsi que CXCL12 et CXCL14 régulent la différentiation du TC au cours du développement du membre. De plus, nous avons établit que la voie de signalisation BMP et les forces mécaniques régulent négativement l’expression des chimiokines CXCL12 et CXCL14. Ces résultats caractérisent pour la première fois l’implication de CXCL12 et CXCL14 dans la différentiation du TC.La deuxième partie de ce travail de thèse a visé à caractériser le rôle paracrine de CXCL12 et CXCL14 sur le développement musculaire. Nous avons pu observé que l’un des récepteur de CXCL12, CXCR7, est exprimé dans les cellules musculaires souches et différenciées, dans les ailes d’embryons de poulets. En utilisant des approches de gains et pertes de fonctions, d’une part in vitro dans des cultures primaires de myoblastes de poulets, nous avons montrés que CXCR7 favorise la myogénèse, notamment en régulant la myogénèse, tandis que CXCL12 n’a pas d’impact sur la différentiation musculaire in vitro. De plus nous avons pu constater que CXCL14 inhibe la myogénèse in vitro. Finalement, in vivo, nous avons observé que la surexpression des chimiokines entraîne un développement anormal des muscles tandis que l’expression du récepteur CXCR7 tronqué favorise le développement musculaire, soulignant l’importance de la signalisation CXCL12/14 dans le processus de morphogénèse musculaire medié par le TC. Ces résultats constituent la première démonstration d’une fonction paracrine du TC dans la morphogénèse musculaire via les chimiokines CXCL12 et CXCL14. / Skeletal muscle development mostly relies on intrinsic capacities of muscle progenitors to proliferate and differentiate. However, extrinsic signals arising from non-myogenic cells also contribute to the establishment of functional skeletal muscles. The aim of this PhD project was to investigate the role of connective-tissue (CT) on the development of skeletal muscle, using the chick embryonic limb as a model. We particularly investigated the influence of the two chemokines CXCL12 and CXCL14, which have been previously shown as expressed in limb mesenchyme giving rise to the different types of CTs during development. The involvement of CXCL12 and CXCL14 in limb CT differentiation was studied, as well as the role of these chemokines in skeletal muscle development mediated by CT. We first showed that CXCL12 and CXCL14 display distinct restricted expression patterns in limb CT of chick embryos and demonstrated that CXCL12 promotes the expression of OSR1, OSR2 and COL3A1 genes, three markers of irregular CT, while CXCL14 enhances the expression of a regular CT gene, SCX. In addition, the expression of CXCL12, CXCL14 and their putative CT target genes were all negatively regulated by the anti-fibrotic BMP signalling, but also in the absence of musculoskeletal mechanical forces. These results show for the first time the involvement of CXCL12 and CXCL14 chemokines in the differentiation of CTs. The putative role of both chemokines on CT-mediated myogenesis was then analysed. We observed that CXCR7, one CXCL12 receptor, was expressed both in muscle progenitors and differentiated muscle cells in embryonic chick limbs. Using gain- and loss-of-function approaches in primary cultures of chick limb myoblasts, we revealed that CXCR7 promoted myogenesis by regulating muscle cell fusion, while CXCL12 did not influence muscle differentiation. CXCL14 dramatically inhibits in vitro myogenesis. Functional assays performed in chick embryonic forelimbs in vivo demonstrate that overexpression of CXCL12, CXCR7 or a dominant-negative form of CXCR7 all resulted in abnormal and mispatterned muscles in chick limbs. Similarly, CXCL14 overexpression in chick limb in vivo led to profound anomalies in muscle differentiation. All together, our results demonstrate an essential contribution of CXCL12 and CXCL14 chemokines in CT differentiation and in CTmediated muscle development in embryonic limb. / Die Entwicklung der Skelettmuskulatur beruht auf den Fähigkeiten von myogenen Progenitorzellen. Im Laufe der Entwicklung proliferieren diese, differenzieren zu Myoblasten, die schließlich zu Muskelfasern fusionieren. Zur Bildung der embryonalen Muskulatur werden jedoch darüber hinaus extrinsische Signale von nicht-myogenen Zellen, vor allem Zellen des Bindegewebes, benötigt. Das Ziel dieser Arbeit war, die Rolle des Bindegewebes in der embryonalen Muskelentwicklung der Extremitäten des Hühnerembryos als Modell genauer zu untersuchen. Speziell wurde hier der Einfluss zweier Chemokine untersucht, CXCL12 und CXCL14, von denen bereits vorher gezeigt wurde, dass sie im Mesenchym der sich entwickelnden Extremität exprimiert sind. In dieser Arbeit wurde die Rolle dieser Chemokine sowohl für die Differenzierung des Bindegewebes selbst, wie auch deren Einfluss auf die Muskeldifferenzierung analysiert. Es konnte gezeigt werden, dass sowohl CXCL12 als auch CXCL14 distinkte regionale Expressionsmuster im Bindegewebe der Extremität aufweisen. Funktionell erhöht CXCL12 die Expression von OSR1, OSR2 und COL3A1, dreier Marker für irreguläres Bindegewebe, während CXCL14 die Expression eines Schlüsselmarkers für reguläres Bindegewebe (Sehne), SCX, erhöht. Die Expression von CXCL12 und CXCL14 selbst, wie auch die Expression ihrer putativen Zielgene, wurden demgegenüber durch Stimulation des antifibrotisch wirkenden BMP Signalweges negativ reguliert. Zudem bewirkte eine experimentell induzierte Muskelparalyse im Hühnerembryo eine Herunterregulation von CXCL12 und CXCL14, was bedeutet, dass die Expression beider Gene abhängig von mechanischen Signalen ist. Diese Ergebnisse involvieren zu ersten Mal die Chemokine CXCL12 und CXCL14 in die Differenzierung des Bindegewebes. Im Folgenden wurde die mögliche Rolle beider Chemokine in der Muskelentwicklung analysiert. Es wurde zunächst gezeigt, dass CXCR7, ein Rezeptor für CXCL12, in Muskelvorläufern wie auch differenzierten Muskelzellen in der Extremität des Hühnerembryos exprimiert wird. Durch Funktionsgewinn und –verlust Versuche in primären Myoblasten aus Hühnerembryos konnte gezeigt werden, dass CXCR7 die Muskelbildung durch die Beeinflussung der Zellfusion fördert, während CXCL12 keinen Einfluss hatte. Demgegenüber zeigte CXCL14 eine starke Inhibition des Myogenese in vitro. Die Misexpression von CXCL12, CXCR7 sowie dominant-negative Versionen von CXCR7 in vivo bewirkten eine abnormale Musterbildung der Extremitäten-Muskulatur. Genauso bewirkte die Misexpression von CXCL14 deutliche Veränderungen der Muskeldifferenzierung. Zusammenfassend konnte eine Funktion der Chemokine CXCL12 und CXCL14 in der Differenzierung des Bindegewebes sowie im nicht-Zell autonomen Einfluss des Bindegewebes auf die Muskelentwicklung gezeigt werden.

During the last five years mediated activism dedicated to LGBT+ issues in Belarus has flourished despite restrictive context: several new online initiatives, including a media project, have been launched. The current study investigates how one of the most politically underprivileged and marginalized groups – LGBT+ activists – make use of online social media to advocate for positive social and political modification in the Belarusian society. By collecting interviews with activists as a primary source of lived experiences, applying thematical analysis on the data from 13 interviews, and then contributing with netnography-informed content analysis as an instrument to analyse 34 posts written in February of 2018 on the personal Facebook pages of the same activists, the current research examines patterns of experiences surrounding participation in mediated LGBT+ activism. The power dynamics and the influence of the repressive context on the practices of mediated activism are analysed through feminist critical discourse analysis with specific focus on heteronormativity as a key-concept of imposing power on marginalized identities. Four global themes emerged in the result of the analysis: 1) heteronormativity and state control; 2) identity as “doing”; 3) the “other” activism, and 4) social change as individual transformation. Topics of heteronormativity, homophobia, hate-crime and violence turned out to be most present in the posts produced by the activists. It was found that in the restrictive spaces mediated activism and social media, instead of serving as tools for mass outreach and mobilization, endanger activists engaged in LGBT+ issues. Burnout, risk of poverty, emotional and physical assaults, and exposure to social sanctions are happening to activists because of their presence online, and there are extremely limited tools to combat these consequences of publicity. In Belarusian context, the shrinking space for civil society and limited political opportunities outweigh the potential of online social media, lower their impact and determine prospects of social change in such a way, when viral organizing or structural transformations become extremely limited.

As a result of the #MeToo movement in Sweden, #sistabriefen was created to represent the women, non-binaries and trans-persons working within the communications industry. This study analyzes the dynamics and identities of the #sistabriefen group members on their private social media platform. The analysis incorporates The Logic of Connective Action by Bennett and Segerberg (2012), and two complementary Social Identity Perspectives; Social Identity Theory and Self-Categorization Theory (Hogg &amp; Terry, 2001; Hogg &amp; Reid, 2006). The study consisted of 23 interview participants, and a qualitative content analysis over the course of five months. This research assesses how members are motivated to participate in the #sistabriefen group, how they identify themselves within the group, and how the group features affect members’ involvement. The findings of the research indicated that digital social movements have the potential to effectively mobilize social change.

Made available in DSpace on 2014-06-11T19:27:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-03-28Bitstream added on 2014-06-13T19:35:58Z : No. of bitstreams: 1 mastrantonio_ss_me_arafo.pdf: 1036636 bytes, checksum: 37dfc0cbf33459b88178c9c9a46516cb (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo deste trabalho foi avaliar a compatibilidade biológica in vivo de materiais odontológicos para remoção químico-mecânica do tecido cariado. Para isto, foram utilizados 32 camundongos que receberam no tecido conjuntivo subcutâneo o implante de tubos de polietileno preenchidos com Carisolv, Papacárie® e base de gel. Os animais foram sacrificados 3, 7, 20 e 30 dias após a cirurgia de implante, sendo os espécimes obtidos processados e submetidos à análise histológica. Os resultados mostraram que o Carisolv provocou uma inativação do metabolismo celular no período inicial, seguida de resposta inflamatória no período final. O grupo do Papacárie® manteve uma inflamação moderada até os 20 dias, que diminuiu de intensidade aos 30 dias e a base do gel provocou reação inflamatória discreta inicial, que aumentou aos 30 dias. Pôde-se concluir que o Carisolv, o Papacárie® e a base de gel são biocompatíveis com o tecido conjuntivo, porém as alterações provocadas por estes materiais são estatisticamente diferentes. / The aim of this work was evaluate biological compatibility in vivo of dental materials to chemo-mechanical removal of caries. This study was conducted to observe in thirty-two mice subcutaneous connective tissue reaction to the implanted polyethylene tubes filled with Carisolv, Papacárie® and gel base. The animals were sacrificed 3, 7, 20 and 30 days after the implantation procedure. The implant sites were excised and prepared for histological evaluation. The results showed that Carisolv inactivated the cellular metabolism in the initial period, followed by inflammatory response in the final period. The group of Papacárie® maintained moderate inflammation until 20 days, that reduced the intensity in 30 days and the gel base provoked initial discrete inflammatory reaction, that increased in 30 days. Carisolv, Papacárie® and gel base are biocompatible with connective tissue, although alterations caused for these materials are statistically different.

Submitted by Thiago Lazzari (thiagolazzari@hotmail.com) on 2017-08-08T20:18:07Z No. of bitstreams: 1 Dissert. Thaigo R Lazzari.pdf: 1677577 bytes, checksum: 04f7da9c131dd8b74e560d57e38fd05a (MD5) / Approved for entry into archive by LUIZA DE MENEZES ROMANETTO (luizamenezes@reitoria.unesp.br) on 2017-08-11T14:18:01Z (GMT) No. of bitstreams: 1 lazzari_tr_me_sjc.pdf: 1677577 bytes, checksum: 04f7da9c131dd8b74e560d57e38fd05a (MD5) / Made available in DSpace on 2017-08-11T14:18:01Z (GMT). No. of bitstreams: 1 lazzari_tr_me_sjc.pdf: 1677577 bytes, checksum: 04f7da9c131dd8b74e560d57e38fd05a (MD5) Previous issue date: 2017-06-07 / Os implantes dentários têm sido utilizados desde meados da década de 50, e inúmeros estudos garantem a confiabilidade para sua utilização, dessa forma o implante tem se tornado uma prática comum entre os cirurgiões dentistas. À medida que sua utilização vem crescendo, suas complicações também aumentaram, principalmente quando instalados em áreas estéticas, onde há deficiência de tecido conjuntivo perimplantar. O objetivo deste estudo clínico controlado randomizado foi avaliar o aumento do volume de tecido conjuntivo perimplantar em implantes instalados em áreas estéticas com a utilização de enxerto de tecido conjuntivo. Para este estudo, foi utilizada uma amostra de 42 indivíduos com necessidade de implantes em áreas estéticas, onde o grupo teste (n=20) recebeu enxerto de tecido conjuntivo sobre os implantes e o grupo controle (n=22) recebeu apenas o implante dental sem a colocação de enxerto de tecido conjuntivo. Para análise do aumento do tecido perimplantar foram realizadas medidas clínicas no baseline, trans-operatório e pós-operatório de 4 meses. Após 4 meses o grupo teste apresentou diferença significativa no aumento de tecido conjuntivo, uma média 3,35±1,08mm / 3,62±1,08mm na vestibular e sobre o rebordo respectivamente, quando comparado ao grupo controle 2,08±0,62mm/ 2,51±0,53mm na vestibular e sobre o rebordo respectivamente. Houve diferença significativa na diminuição da deiscência óssea vestibular para o grupo teste uma média de 0,4±0,8mm contra 1,0±0,8mm do grupo controle (p<0,05). Não houve diferença significativa quanto a dor relatada pelos pacientes e a quantidade de analgésicos ingeridos. Pode-se concluir que após 4 meses o grupo teste apresentou aumento no volume de tecido conjuntivo, diminuição da deiscência óssea vestibular e os pacientes do grupo teste não sentiram uma dor maior que o grupo controle. / Dental implants have been used since 1950, and as numerous studies ensure reliability for its use, they implant have become a common practice among dentists. However , complications have also risen from its increased application, especially when they are installed in esthetic areas, where there is deficiency of periimplant soft tissue. The purpose of this randomized controlled trial was to evaluate the increase in volume periimplantar soft tissue esthetic areas with the use of connective tissue graft. Individuals in need of implants in esthetic areas were included, and divided in two groups: test group (n = 20) received a tissue graft over the implants and the control group (n = 22) received only dental implant without the connective tissue graft placement. For the analysis of periimplantar tissue augmentation, baseline, trans-operative and postoperative measurements were performed. After 4 months, the test group presented a significant difference in mean connective tissue augmentation, with 3.35 ± 1.08 mm in the vestibular area and 3.62 ± 1.08 mm on the ridge when compared to the control group, with 2.08 ± 0 62 mm in the vestibular area and 2.51 ± 0.53mm on the ridge. There was a significant difference in the reduction of vestibular bone dehiscence for the test group, with a mean of 0.4 ± 0.8 mm versus 1.0 ± 0.8 mm in the control group (p <0.05). There was no significant difference in pain reported by patients and the amount of post-operative analgesics ingested taken. It can be concluded that after 4 months the use of connective tissue graft test group resulted in an increase in connective tissue volume, decreased vestibular bone dehiscence with similar amount of post-operative pain than the control group.