published_or_final_version / Electrical and Electronic Engineering / Doctoral / Doctor of Philosophy

Hoje em dia existe um consenso de que a prevenção e o tratamento de doenças periodontais são importantes na redução de mortalidade e morbidade associadas à doença cardíaca, e uma correlação entre aterosclerose e doença periodontal ainda é desconhecida. Neste estudo, a presença de Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, Prevotella nigrescens, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Campylobacter rectus e Enterococcus faecalis em placas de ateroma de artérias coronárias foram detectadas. Trinta pacientes programados para endarterectomia, 28 pacientes com periodontite crônica e 2 sem doença periodontal foram incluídos no estudo. DNA bacteriano foram extraídos dos ateromas e a presença de bactérias periodontais foram detectadas através da reação em cadeia da polimerase. DNA bacteriano periodontal foi encontrado em 28 amostras de ateroma de pacientes periodontais: P. gingivalis em 14 (50%); P. nigrescens em 4 (14,3%), P. intermedia em 3 (10,7%); E. faecalis em 3 (10,7%), T. forsythia em 2 (7,1%); A. actinomycetemcomitans em 2 (7,1%); C. rectus em 2 (7,1%); T. denticola em 1 (3,6%); e P. endodontalis em 1 (3,6%). Fusobacterium nucleatum não foi encontrado em nenhuma das amostras analisadas. Em duas amostras de ateroma de pacientes sem doença periodontal, P. gingivalis e E. faecalis foram observadas em uma amostra e P. intermedia em ambas as amostras. Nossos resultados sugerem que a presença de P. gingivalis em ateroma de artérias coronárias pode apresentar um envolvimento com aterosclerose e parecendo ser um microrganismo da relação aterosclerose e periodontite crônica. / Nowadays there is a consensus that prevention and treatment of periodontal diseases are important in the reduction of mortality and morbidity associated to cardiovascular diseases, and a correlation between atherosclerosis and periodontal disease is still unclear. In this study, the presence of Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, Prevotella nigrescens, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Campylobacter rectus and Enterococcus faecalis in atheroma plaques from coronary arteries was determined. Thirty patients scheduled for endarterectomy, 28 patients with chronic periodontitis and 2 without periodontal diseases were included in this study. Bacterial DNA was extracted from atheroma and periodontal bacteria detection was done by polymerase chain reaction. Periodontal bacterial DNA was found in 28 atheroma samples from periodontal patients: P. gingivalis in 14 (50%); P. nigrescens in 4 (14.3%); P. intermedia in 3 (10.7%); E. faecalis in 3 (10.7%), T. forsythia in 2 (7.1%); A. actinomycetemcomitans in 2 (7.1%); C. rectus in 2 (7.1%); T. denticola in 1 (3.6%); and P. endodontalis in 1 (3.6%) samples. Fusobacterium nucleatum was not detected in any atheroma analyzed. In two atheroma samples from patients without periodontal disease, P. gingivalis and E. faecalis were observed in one sample and P. intermedia in both samples. Our results suggest that the presence of P. gingivalis in atheroma from coronary arteries may be involved in atherosclerosis, and it appears to be a relationship microorganism atherosclerosis and chronic periodontitis.

Hoje em dia existe um consenso de que a prevenção e o tratamento de doenças periodontais são importantes na redução de mortalidade e morbidade associadas à doença cardíaca, e uma correlação entre aterosclerose e doença periodontal ainda é desconhecida. Neste estudo, a presença de Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, Prevotella nigrescens, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Campylobacter rectus e Enterococcus faecalis em placas de ateroma de artérias coronárias foram detectadas. Trinta pacientes programados para endarterectomia, 28 pacientes com periodontite crônica e 2 sem doença periodontal foram incluídos no estudo. DNA bacteriano foram extraídos dos ateromas e a presença de bactérias periodontais foram detectadas através da reação em cadeia da polimerase. DNA bacteriano periodontal foi encontrado em 28 amostras de ateroma de pacientes periodontais: P. gingivalis em 14 (50%); P. nigrescens em 4 (14,3%), P. intermedia em 3 (10,7%); E. faecalis em 3 (10,7%), T. forsythia em 2 (7,1%); A. actinomycetemcomitans em 2 (7,1%); C. rectus em 2 (7,1%); T. denticola em 1 (3,6%); e P. endodontalis em 1 (3,6%). Fusobacterium nucleatum não foi encontrado em nenhuma das amostras analisadas. Em duas amostras de ateroma de pacientes sem doença periodontal, P. gingivalis e E. faecalis foram observadas em uma amostra e P. intermedia em ambas as amostras. Nossos resultados sugerem que a presença de P. gingivalis em ateroma de artérias coronárias pode apresentar um envolvimento com aterosclerose e parecendo ser um microrganismo da relação aterosclerose e periodontite crônica. / Nowadays there is a consensus that prevention and treatment of periodontal diseases are important in the reduction of mortality and morbidity associated to cardiovascular diseases, and a correlation between atherosclerosis and periodontal disease is still unclear. In this study, the presence of Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, Prevotella nigrescens, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Campylobacter rectus and Enterococcus faecalis in atheroma plaques from coronary arteries was determined. Thirty patients scheduled for endarterectomy, 28 patients with chronic periodontitis and 2 without periodontal diseases were included in this study. Bacterial DNA was extracted from atheroma and periodontal bacteria detection was done by polymerase chain reaction. Periodontal bacterial DNA was found in 28 atheroma samples from periodontal patients: P. gingivalis in 14 (50%); P. nigrescens in 4 (14.3%); P. intermedia in 3 (10.7%); E. faecalis in 3 (10.7%), T. forsythia in 2 (7.1%); A. actinomycetemcomitans in 2 (7.1%); C. rectus in 2 (7.1%); T. denticola in 1 (3.6%); and P. endodontalis in 1 (3.6%) samples. Fusobacterium nucleatum was not detected in any atheroma analyzed. In two atheroma samples from patients without periodontal disease, P. gingivalis and E. faecalis were observed in one sample and P. intermedia in both samples. Our results suggest that the presence of P. gingivalis in atheroma from coronary arteries may be involved in atherosclerosis, and it appears to be a relationship microorganism atherosclerosis and chronic periodontitis.

Statins induce beneficial vascular effects. How statins induce beneficial vascular effects is yet to be determined. Here we examine Simvastatin and vascular endothelial growth factor (VEGF) acting through the phosphoinositide 3-kinase (PI3K) pathway in human coronary artery endothelial cells (HCAEC). While Simvastatin and VEGF both activated mediators in the PI3K pathway, the proteins and the rates of activation were not always consistent. This suggests that although Simvastatin and VEGF share a common PI3K pathway in HCAEC and similar vascular effects, the agonists diverge in the induction of cellular signaling cascades. Simvastatin also was shown to induce phosphoinositide 3, 4, 5-triphosphate (PIPS) organization and PI3K p110 gamma (y) perinuclear localization. Beneficial, non-lipid lowering effects of statins may occur through the PI3K pathway through activation of distinct mediators from those of VEGF. Better understanding of the pathways associated with statins is necessary for the discovery of better treatments for cardiovascular disease (CVD). / Department of Biology

Tez (Uzmanlık) - Süleyman Demirel Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, 2003. / Kaynakça var.

Tez (Tıpta Uzmanlık) - Süleyman Demirel Üniversitesi, Tıp Fakültesi, Kardiyoloji Anabilim Dalı, 2004. / Bibliyografya var.

Tez (Tıpta Uzmanlık) - Süleyman Demirel Üniversitesi, Tıp Fakültesi, Kardiyoloji Anabilim Dalı, 2005. / Bibliyografya var.

Tez (Tıpta Uzmanlık) - Süleyman Demirel Üniversitesi, Tıp Fakültesi, Kardiyoloji Anabilim Dalı, 2006. / Bibliyografya var.

Thesis (Ph. D.)--West Virginia University, 2009. / Title from document title page. Document formatted into pages; contains viii, 109 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references.

Thesis (M.D.) - University of Glasgow, 2009. / M.D. thesis submitted to the Division of Cardiovascular and Medical Sciences, University of Glasgow, 2009. Includes bibliographical references. Print version also available.