Orientadores: Paulo Sergio Moreira Carvalho de Oliveira, Jose Roberto Trigo / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-11T11:43:38Z (GMT). No. of bitstreams: 1 Silveira_HenriqueCesarPelicci_M.pdf: 1601394 bytes, checksum: fd0e1ab69ffeaaa903956975abcf14ec (MD5) Previous issue date: 2008 / Mestrado / Mestre em Ecologia

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In response to the drastic decline of the European eel (Anguilla anguilla (L.)) fisheries have been reduced and elvers are stocked in areas where natural abundances are low. Are these measures adequate? To answer different aspects of this question, we have analysed more than a century of eel tagging, using both traditional and more novel capture – recapture analyses. Based on these long-term data, we have evaluated the impact of the Swedish eel coastal fisheries using Survival analysis. Our analysis indicates that the fishing mortality just prior the 2009 fishing restrictions were in the order of 10%. More recent tagging programs have focused on issues related to the fate of stocked fish. If and how they migrate out of the Baltic Sea and further on towards the Atlantic Ocean. Both earlier and our new studies reveal that all eels recaptured on the Swedish East Coast, no matter of their origin, migrate at a reasonable speed and direction towards the outlets of the Baltic Sea. Even though it is sometimes difficult to determine their origin, our analyses indicate that stocked fish were scarce among the recaptures. In an experiment on the Swedish West Coast, we knew the individuals’ origin (stocked or wild) and they had similar migration patterns. In contrast, silver eel in Lake Mälaren – assumed to have been stocked as elvers or bootlace eels – seemed to have difficulties in finding the outlets. Instead they overwintered and lost weight. However, weight losses are also significant among non-stocked individuals in the Baltic Sea, both if they overwinter and if they appear to be on their way out from the area. It remains an open question whether eels from the Baltic region in general, and whether the overwintered fish in particular, manage to reach the spawning area in the Atlantic Ocean. Based on current knowledge, I advocate invoking the precautionary approach and to concentrate Swedish eel stockings to the West Coast and allow the young fish to spread out on their own. / <p>At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 2: Manuscript.</p><p> </p>

Sötvattensmusslor av ordningen unionoida är en av världens mest hotade organismgrupper och tjockskalig målarmussla (Unio crassus) klassas som starkt hotad enligt IUCN:s lista över rödlistade arter. Bevarandearbeten har tidigare utförts där tjockskalig målarmussla har återintroducerats till Klingavälsån och Fyleån i södra Sverige. Vid återintroduktion är utgångspunkten genetiskt släktskap, men när den informationen inte finns väljer man att återintroducera den population som är geografiskt närmast. Syftet med studien var att undersöka ifall geografisk avstånd mellan de fyra vattendragen Bråån, Tommarpsån, Klingavälsån och Fyleån påverkar juvenilöverlevnad. Syftet var även att ta reda på vilken av Bråånmusslor och Tommarpsånmusslor som är bäst lämpad för återintroduktion i Fyleån och Klingavälsån. Jag hade följande hypoteser, (1) Bråånmusslor har högst överlevnad i Bråån, följt av Klingavälsån, Fyleån och Tommarpsån och (2) Tommarpsånmusslor har högst överlevnad i Tommarpsån följt av, Fyleån, Klingavälsån och Bråån. Befruktade musslor fångades in från Bråån och Tommarpsån i maj 2016. Musslorna släppte i från sig glochidielarver som sedan användes till att infektera fiskar. Stensimpor (Cottus gobio) elfiskades från Tommarpsån och elritsor (Phoxinus phoxinus) elfiskades från Bråån, därefter infekterades stensimpor med glochidielarver från Tommarpsån och elritsor med glochidielarver från Bråån. Juvenila musslor släppte från fiskarna 25-35 dagar senare och matades under hela sommaren, därefter sattes musslorna ut i boxar i de fyra vattendragen. Överlevnaden för musslorna mättes och dokumenterades vid två tillfällen. Fyra Kruskal-Wallis tester genomfördes där ”överlevnad” jämfördes med ”vattendrag” för Bråånmusslor och Tommarpsånmusslor. Resultatet stämde inte överens med hypotesen för vare sig Bråånmusslor eller Tommarpsånmusslor, förutom att Bråånmusslor hade lägst överlevnad i det mest avlägsna vattendraget vid andra mättillfället. Överlevnaden för Bråånmusslor skilde sig mellan flera av vattendragen men för Tommarpsånmusslor var det ingen skillnad i överlevnad. Bråånmusslor verkade mer lämpliga för återintroduktion i Fyleån jämfört med Klingavälsån och Tommarpsånmusslor verkade inte sämre lämpade för återintroduktion till vare sig Fyleån eller Klingavälsån. Sammanfattningsvis verkade överlevnaden inte generellt bero på geografisk närhet. Troligtvis har faktorer som lokaladaptation, vattenkvalité och sjukdomar även en påverkan på överlevnaden hos juvenila musslor. / Freshwater mussels are one of the most endangered groups of organisms worldwide, with the thick-shelled river mussel (Unio crassus) being classified as critically endangered according to the IUCN:s red list of threatened species. Conservation efforts have been undertaken in southern Sweden, where the thick-shelled river mussel has been reintroduced to Klingavälsån and Fyleån. Genetic relatedness is typically used as the starting point when reintroducing mussels, but if this information is not available, the population closest geographically is chosen. The objective of this study was to investigate whether the distance between four watercourses, namely Bråån, Tommarpsån, Klingavälsån and Fyleån affects juvenile survival and to determine which population of mussels from Bråån and Tommarpsån is best suited for reintroduction to Fyleån and Klingavälsån. The hypotheses are that (1) Bråån mussels have the highest growth rate in Bråån, followed by Klingavälsån, Fyleån and Tommarpsån, and (2) Tommarpsån mussels have the highest growth rate in Tommarpsån, followed by Fyleån, Klingavälsån and Bråån. Fertilized mussels were collected from Bråån and Tommarpsån in May 2016, and the mussels released glochidia larvae, which were used to infect fish. European bullheads (Cottus gobio) were electrofished from Tommarpsån and European minnows (Phoxinus phoxinus) were electrofished from Bråån, and then the European bullheads were infected with glochidia larvae from Tommarpsån and European minnows were infected with glochidia larvae from Bråån. Juvenile mussels let go of the fish 25 – 35 days later and were fed throughout the summer, after which the mussels were placed in boxes in the four watercourses. Survival of the mussels was measured and documented on two occasions. Four Kruskal-Wallis tests were performed where “survival” was compared with “watercourse” for Bråån mussels and for Tommarpsån mussels. The results did not match the hypothesis for either Bråån mussels or Tommarpsån mussels, except for the fact that Bråån mussels had the lowest survival rate in the most remote stream at the second measurement occasion. The survival of the Bråån mussels differed between several of the streams, but for the Tommarpsån mussels there were no significant difference in survival. The Bråån mussels seemed more suitable for reintroduction into Fyleån compared to Klingavälsån, and Tommarpsån mussels did not seem less suitable for reintroduction to either Fyleån or Klingavälsån. In summary, survival did not appear to generally depend on geographic proximity. Factors such as local adaptation, water quality and diseases likely also have an impact on the survival of juvenile mussels.

Die Einschleppung gebietsfremder Parasiten durch den Menschen ist einer der Hauptgründe für das Auftreten neuer Krankheiten in the letzten Jahrzehnten. Neue Wirte sind oftmals anfälliger für diese invasiven Parasiten als die ursprünglichen Wirte. In schwerwiegenden Fällen können invasive Parasiten zu Massensterben und zum Aussterben ihrer neuen Wirte führen. Der ursprüngliche Wirt des Aalparasiten Anguillicola crassus ist der Japanische Aal. In den frühen 1980er Jahren wurde der Parasit in die Population des Europäischen Aals eingeschleppt. Er ist einer der Faktoren, die für den Populationsrückgang des Europäischen Aals verantwortlich sind. Die molekularen Prozesse, die zur stärkeren Anfälligkeit des Europäischen Aals im Vergleich zum Japanischen Aal führen, sind noch nicht zureichend bekannt. Die Analyse transkriptomweiter differenzieller Genexpression von Immungewebe ergab, dass im Europäischen Aal sowohl Immun- als auch Nichtimmungene differenziell exprimiert waren. Dies war im Japanischen Aal nicht der Fall und deutet darauf hin, dass der Europäische Aal eine ineffiziente und kostspielige Immunantwort auslöst. Die Resultate ensprechen jenen die schon in anderen Wirbeltierwirt-invasiven Parasiten-Systemen beobachtet wurden. Ausserdem stützen diese Resultate die Hypothese, dass neuen Wirten eine wirksame Immunantwort fehlt und sie deuten darauf hin, dass Nichtimmunprozesse wesentlich zur höheren Anfälligkeit von neuen Wirten beitragen. Als Reaktion of die negativen Fitnesseffekte können neue Wirte Abwehrmechanismen entwickeln. Im Europäischen Aal entspricht das der Einkapselung von A. crassus. Einkapselung führte zu eine niedrigere Abundanz adulter A. crassus. Dies deutet darauf hin, dass das Einkapseln sich positiv auf die Gesundheit infizierter Aale auswirkt. Jedoch war die Abundanz zweier nativer Parasiten höher in Aalen, die A. crassus einkapselten. Eine verbesserte Abwehr des eingeschleppten Parasiten könnte daher die Abwehr nativer Parasiten beeinträchtigen. / The human-mediated translocation of non-native parasites into foreign regions is one of the primary factors for the emergence of new diseases in recent decades. Novel hosts are often more susceptible to these invasive parasites than the native host. In severe cases, invasive parasites can lead to population declines and extinctions of their novel hosts. The eel parasite Anguillicola crassus is native to the Japanese eel. In the early 1980s it was introduced into the European eel population and is now considered to be one factor contributing to the population decline of its novel host. The underlying molecular processes determining higher susceptibility in the European eel compared to the Japanese eel are not well understood. Using whole-transcriptome differential gene expression analysis of immune organs, I found that genes involved in both immune and non-immune processes were differentially expressed in the European eel but not the Japanese eel, suggestive of an ineffective and costly immune response in the former. These results are in line with those observed between susceptible and resistant hosts in other vertebrate host-invasive parasite systems. Furthermore, the results support the hypothesis that novel hosts lack an effective immune response. The results also suggest that alteration of non-immune processes contributes substantially higher susceptibilities of novel hosts. In response to the negative fitness effects of invasive parasites, novel hosts can evolve coping mechanisms. The European eel has the capacity to encapsulate and kill A. crassus. Using natural infections, I found a lower abundance of adult A. crassus, the most costly parasitic stage in those eels encapsulating the parasite, suggesting that encapsulation can potentially improve health of infected eels. At the same time, the abundance of two native parasites was higher in those eels encapsulating A. crassus. Thus, coping with A. crassus may come at the expense of coping with native parasites.

This thesis focuses on shell shape plasticity of Unio crassus at 12 localities. 1129 shape outlines were used for the purposes of this work. The outlines were gained from 260 individuals in the way of analysing the winter growth lines. The Elliptic Fourier Analysis appeared to be reasonable tool for the bivalves' shells shapes assessment. The winter growth lines' consideration showed great potential for morphometric analyses. The results shows that PC1 and PC2 axes (from principal component analysis of shape coeficients) are growth- dependent. The allometry influence was removed by residual values assessment obtained from generalized linear model (GLM). The age, length and width parameters appears to be favourable combination for general allometry influence removal (by using these parameters as describing variables in GLM model). The results point to significant differences among localities in all assessed parameters. Furthermore, the significant difference in growth speed was approved. Speed growth indicator correlates with shape variables. The localities can be divided into two groups with different growth speed each (Bertalanffy growth constant, length in the third year and age relative length). These groups differs in about 10 mm in average lengths. The most similar localities were "Blanice...

Appien est l’auteur d’une histoire universelle, l’Histoire romaine, allant de la fondation de Rome jusqu’à l’époque de Trajan. Parmi les livres qui la composent, nous pouvons remarquer la présence d’un Livre Parthique. Bien qu’Appien lui-même manifeste à plusieurs reprises l’intention d’écrire un Livre Parthique, la tradition manuscrite ne nous a transmis qu’un texte très problématique, intitulé Παρθική, qui n’est qu’un centon d’extraits tirés du Livre Syriaque d’Appien et des Vies d’Antoine et de Crassus de Plutarque. Il est évident que la nature de ce texte a engendré un vif débat sur son authenticité. C’est pour trancher la question que notre thèse fournit une nouvelle édition critique du texte de la Παρθική, prenant en compte les études les plus récentes sur la tradition manuscrite de l’Histoire Romaine ; mais surtout elle vise à prendre parti sur la question de l’authenticité de ce texte, en étudiant la méthode historiographique d’Appien et l’usage qu’il fait de ses sources. Compte tenu de cette analyse, nous pouvons conclure que la Παρθική n’accomplit pas ce que l’historien s’était fixé et que son contenu ne correspond pas aux informations qu’il avait déjà à sa disposition. C’est pourquoi il ne paraît pas possible de considérer authentique la Παρθική. / Appian’s principle surviving work, known as Roman History, inspects the events occurred from Rome foundation until the reign of the Emperor Trajan. Among its different books, we can notice the Parthian History. Despite Appian’s intention of writing a Parthian History, the test that reached us – called Παρθική – is made only of excerpts from Appian’s Syrian History and from Plutarch’s Life of Crassus and Life of Antony. It is clear that the characteristics of this test have generated an intense debate on its authenticity. Object of this work is to shed light on this issue, writing a new critical edition of Παρθική, that takes into account the latest studies of the Roman History manuscript tradition. Furthermore, this work has the aim of taking a position on the question of authenticity through a deep study of Appian’s historiographical method and his use of sources. From this analysis it results that Παρθική is not adherent to Appian purposes and its contents does not match with the information he had available. For these reasons it doesn’t seems possible to take the position of the book authenticity.