No description available.

No description available.

No description available.

No description available.

No description available.

No description available.

Learning-based multi-view stereo (MVS) has shown promising results in the domain of general 3D reconstruction. However, no work before this thesis has applied learning-based MVS to urban 3D reconstruction from satellite images. In this thesis, learning-based MVS is used to infer depth maps from satellite images. Models are trained on both synthetic and real satellite images from Las Vegas with ground truth data from a high-resolution aerial-based 3D model. This thesis also evaluates different methods for reconstructing digital surface models (DSM) and compares them to existing satellite-based 3D models at Maxar Technologies. The DSMs are created by either post-processing point clouds obtained from predicted depth maps or by an end-to-end approach where the depth map for an orthographic satellite image is predicted.  This thesis concludes that learning-based MVS can be used to predict accurate depth maps. Models trained on synthetic data yielded relatively good results, but not nearly as good as for models trained on real satellite images. The trained models also generalize relatively well to cities not present in training. This thesis also concludes that the reconstructed DSMs achieve better quantitative results than the existing 3D model in Las Vegas and similar results for the test sets from other cities. Compared to ground truth, the best-performing method achieved an L1 and L2 error of 14 % and 29 % lower than Maxar's current 3D model, respectively. The method that uses a point cloud as an intermediate step achieves better quantitative results compared to the end-to-end system. Very promising qualitative results are achieved with the proposed methods, especially when utilizing an end-to-end approach.

No description available.

Single Particle Tracking (SPT) plays an important role in the study of physical and dynamic properties of biomolecules moving in their native environment. To date, many algorithms have been developed for localization and parameter estimation in SPT. Though the performance of these methods is good when the signal level is high and the motion model simple, they begin to fail as the signal level decreases or model complexity increases. In addition, the inputs to the SPT algorithms are sequences of images that are cropped from a large data set and that focus on a single particle. This motivates us to seek machine learning tools to deal with that initial step of extracting data from larger images containing multiple particles. This thesis makes contributions to both data extraction question and to the problem of state and parameter estimation. First, we build upon the Expectation Maximization (EM) algorithm to create a generic framework for joint localization refinement and parameter estimation in SPT. Under the EM-based scheme, two representative methods are considered for generating the filtered and smoothed distributions needed by EM: Sequential Monte Carlo - Expectation Maximization (SMC-EM), and Unscented - Expectation Maximization (U-EM). The selection of filtering and smoothing algorithms is very flexible so long as they provide the necessary distributions for EM. The versatility and reliability of EM based framework have been validated via data-intensive modeling and simulation where we considered a variety of influential factors, such as a wide range of {\color{red}Signal-to-background ratios (SBRs)}, diffusion speeds, motion blur, camera types, image length, etc. Meanwhile, under the EM-based scheme, we make an effort to improve the overall computational efficiency by simplifying the mathematical expression of models, replacing filtering/smoothing algorithms with more efficient ones {\color{purple} (trading some accuracy for reduced computation time)}, and using parallel computation and other computing techniques. In terms of localization refinement and parameter estimation in SPT, we also conduct an overall quantitative comparison among EM based methods and standard two-step methods. Regarding the U-EM, we conduct transformation methods to make it adapted to the nonlinearities and complexities of measurement model. We also extended the application of U-EM to more complicated SPT scenarios, including time-varying parameters and additional observation models that are relevant to the biophysical setting. The second area of contribution is in the particle detection and extraction problem to create data to feed into the EM-based approaches. Here we build Particle Identification Networks (PINs) covering three different network architectures. The first, \PINCNN{}, is based on a standard Convolutional Neural Network (CNN) structure that has previously been successfully applied in particle detection and localization. The second, \PINRES, uses a Residual Neural Network (ResNet) architecture that is significantly deeper than the CNN while the third, \PINFPN{}, is based on a more advanced Feature Pyramid Network (FPN) that can take advantage of multi-scale information in an image. All networks are trained using the same collection of simulated data created with a range of SBRs and fluorescence emitter densities, as well as with three different Point Spread Functions (PSFs): a standard Born-Wolf model, a model for astigmatic imaging to allow localization in three dimensions, and a model of the Double-Helix engineered PSF. All PINs are evaluated and compared through data-intensive simulation and experiments under a variety of settings. In the final contribution, we link all above together to create an algorithm that takes in raw camera data and produces trajectories and parameter estimates for multiple particles in an image sequence.

Deep Vein Thrombosis, a potentially fatal event, occurs when a blood clot forms within the deep veins of the body. This most frequently manifests in the lower extremities. The goal of this research was to build an inexpensive device that could apply therapeutic compressive pressure to the lower leg to aid in the prevention of deep vein thrombosis using only mechanical input from the user. Several different prototypes were designed and built with varying degrees of success. Characterization of the final prototype required calibration of pressure and force measurement sensors. Additionally, a mathematical model was developed in order to predict how changes in the design of the device, as well as differing sizes and shapes of lower legs, would impact the amount of applied pressure. The predictions of this mathematical model were found to be substantially larger when compared against empirical data. However, there is evidence to indicate that the final prototype could be minimally altered to apply ample therapeutic pressure. / Bioengineering