The objective of this research was to study relationship(s) of ethnicity, physical activity and diet with adiposity development in Aboriginal youth. To meet this objective, three separate, yet inter-related studies were undertaken: 1) to comprehensively assess adiposity in Aboriginal youth and their age, sex and maturity matched Caucasian peers; 2) to assess the role of ethnicity and sex on physical activity (PA) levels and identify the proportion of Aboriginal youth meeting international recommendations; and 3) to explore relationships of ethnicity, physical activity and diet with adiposity. In study one, it was hypothesized that Aboriginal youth would have greater adiposity than their Caucasian peers. While much of the research to date has focused on body mass index, this investigation used DXA and waist circumference to show that Aboriginal youth had greater total and central adiposity in comparison to their Caucasian counterparts.<p> Study two examined physical activity behaviors of Canadian Aboriginal youth in relation to ethnicity and sex. It was hypothesized that the physical activity levels of Aboriginal youth would be lower than their Caucasian peers; that the majority of Aboriginal youth would not meet PA and T.V. viewing recommendations; and that Aboriginal boys would have higher adjusted physical activity energy expenditures than girls. Findings indicated that physical activity levels of the two ethnic groups were generally comparable, that Aboriginal boys had greater activity energy expenditures than girls, and that a greater percentage of boys were meeting the international recommendations for physical activity and T.V. viewing. Study three built upon the first two investigations, to explore relationships of physical activity and diet with adiposity in Aboriginal youth. It was hypothesized that when age, size and maturity, and their interactions were accounted for, diet and physical activity variables would be related to adiposity (waist circumference, total body and trunk fatness). Results indicated physical activity was inversely related to adiposity level, independent of biological factors. Although energy intake was the sole dietary variable related to the adiposity measures, descriptives showed the eating behaviors (i.e., consumption of fruits and vegetables, sugar sweetened beverages and other foods) of the Aboriginal youth were sub-optimal when compared to current recommendations. This research project is unique because it comprehensively assessed adiposity, diet and physical activity, and the relationships between these variables, in a relatively large sample of Aboriginal boys and girls. Furthermore, these relationships were established using a variety of measures (i.e., DXA, waist circumference, height and weight) while controlling for biological confounders. Overall, the results highlight the urgent need to promote physical activity and healthy eating in Aboriginal youth and set the stage for future research.

Sodium restriction is the primary dietary therapy for individuals with heart failure (HF); however, there is little information available to support or refute the use of sodium restriction to manage HF. The overall goal of this work was to generate data related to dietary sodium in patients with chronic HF that would contribute to the development of evidence-based guidelines. The specific objectives were to investigate the optimal methods for measuring sodium intake in HF, to describe the habitual consumption of sodium and other nutrients in HF, and to evaluate the relationship between sodium intake and clinical outcomes in HF. We studied stable ambulatory HF patients who were optimally medicated and participating in multidisciplinary HF programs. We determined that: (1) a strong relationship exists between 24-hour urine collections and food records for sodium intake assessment in non-HF cardiac patients and HF patients not taking loop diuretics. However, the relationship between urinary sodium excretion and sodium intake in HF patients taking loop diuretics was disturbed, suggesting that food records may be a better method for estimating sodium intake in this group. (2) Mean sodium intake in HF and non-HF cardiac patients was similar, and approximately half of patients in each group had sodium intake levels that exceeded the Dietary Reference Intakes tolerable upper level of 2300 mg/d. We also found that both groups had inadequate intakes of several nutrients, including potassium, calcium, magnesium, folate, and vitamin D and E. (3) Finally, we showed that a high sodium diet (>2800 mg/day) in HF was associated with risk of acute decompensated HF, all-cause hospitalization, and all-cause mortality over a median 3 year follow-up period. This is the first published study that prospectively related sodium intake to clinical outcomes in HF. In summary, these data provide novel contributions related to the measurement of sodium intake that can be used in clinical or academic settings. We also describe inadequacies in intake of several vitamins and minerals, which could be addressed through dietary counselling. Finally, we importantly offer insight into a threshold of sodium intake (>2800 mg/day) that could contribute to adverse clinical outcomes in HF.

Sodium restriction is the primary dietary therapy for individuals with heart failure (HF); however, there is little information available to support or refute the use of sodium restriction to manage HF. The overall goal of this work was to generate data related to dietary sodium in patients with chronic HF that would contribute to the development of evidence-based guidelines. The specific objectives were to investigate the optimal methods for measuring sodium intake in HF, to describe the habitual consumption of sodium and other nutrients in HF, and to evaluate the relationship between sodium intake and clinical outcomes in HF. We studied stable ambulatory HF patients who were optimally medicated and participating in multidisciplinary HF programs. We determined that: (1) a strong relationship exists between 24-hour urine collections and food records for sodium intake assessment in non-HF cardiac patients and HF patients not taking loop diuretics. However, the relationship between urinary sodium excretion and sodium intake in HF patients taking loop diuretics was disturbed, suggesting that food records may be a better method for estimating sodium intake in this group. (2) Mean sodium intake in HF and non-HF cardiac patients was similar, and approximately half of patients in each group had sodium intake levels that exceeded the Dietary Reference Intakes tolerable upper level of 2300 mg/d. We also found that both groups had inadequate intakes of several nutrients, including potassium, calcium, magnesium, folate, and vitamin D and E. (3) Finally, we showed that a high sodium diet (>2800 mg/day) in HF was associated with risk of acute decompensated HF, all-cause hospitalization, and all-cause mortality over a median 3 year follow-up period. This is the first published study that prospectively related sodium intake to clinical outcomes in HF. In summary, these data provide novel contributions related to the measurement of sodium intake that can be used in clinical or academic settings. We also describe inadequacies in intake of several vitamins and minerals, which could be addressed through dietary counselling. Finally, we importantly offer insight into a threshold of sodium intake (>2800 mg/day) that could contribute to adverse clinical outcomes in HF.

The impact of habitually consuming a high protein (HP) diet at the upper limit of the acceptable macronutrient distribution range (AMDR) on kidney health is unknown. The current study was designed to test the hypothesis that long-term consumption of a diet providing 35% of energy as protein will have negative consequences on renal health, as assessed in a pig model. Methods: Adult female, non-pregnant, commercial pigs (Genesus) were randomized to receive either NP (15% energy from protein) or HP (35% energy from protein) isocaloric diets for either 4 or 8 months. Diets contained whole protein sources with an animal: plant ratio of 2:1 in the NP diet to mimic the average Canadian diet. The increased protein in the HP diet was achieved by increasing egg and dairy protein sources. Body composition was measured by dual-energy X-ray absorptiometry. Glomerular volume and kidney fibrosis were evaluated on kidney sections by quantitative image analysis. The inflammatory marker monocyte chemoattractant protein-1 (MCP-1) and the growth factor transforming growth factor beta-1(TGFβ1) were assessed in renal tissue using commercial ELISA kits. Results: Pigs given the HP diet had lower body weights and percentage of body fat. Pigs consuming the HP diet had significantly higher glomerular filtration rates (GFR) and larger kidneys. Renal MCP-1 levels and renal fibrosis also were significantly higher in pigs given the HP diet, while proteinuria and renal TGFβ1 expression did not differ. Conclusion: These findings suggest that, despite the potential benefit of the HP diet on body composition, long-term intakes of protein at the upper limit of the AMDR may compromise renal health in healthy female pigs. / October 2008

The objective of this research was to study relationship(s) of ethnicity, physical activity and diet with adiposity development in Aboriginal youth. To meet this objective, three separate, yet inter-related studies were undertaken: 1) to comprehensively assess adiposity in Aboriginal youth and their age, sex and maturity matched Caucasian peers; 2) to assess the role of ethnicity and sex on physical activity (PA) levels and identify the proportion of Aboriginal youth meeting international recommendations; and 3) to explore relationships of ethnicity, physical activity and diet with adiposity. In study one, it was hypothesized that Aboriginal youth would have greater adiposity than their Caucasian peers. While much of the research to date has focused on body mass index, this investigation used DXA and waist circumference to show that Aboriginal youth had greater total and central adiposity in comparison to their Caucasian counterparts.<p> Study two examined physical activity behaviors of Canadian Aboriginal youth in relation to ethnicity and sex. It was hypothesized that the physical activity levels of Aboriginal youth would be lower than their Caucasian peers; that the majority of Aboriginal youth would not meet PA and T.V. viewing recommendations; and that Aboriginal boys would have higher adjusted physical activity energy expenditures than girls. Findings indicated that physical activity levels of the two ethnic groups were generally comparable, that Aboriginal boys had greater activity energy expenditures than girls, and that a greater percentage of boys were meeting the international recommendations for physical activity and T.V. viewing. Study three built upon the first two investigations, to explore relationships of physical activity and diet with adiposity in Aboriginal youth. It was hypothesized that when age, size and maturity, and their interactions were accounted for, diet and physical activity variables would be related to adiposity (waist circumference, total body and trunk fatness). Results indicated physical activity was inversely related to adiposity level, independent of biological factors. Although energy intake was the sole dietary variable related to the adiposity measures, descriptives showed the eating behaviors (i.e., consumption of fruits and vegetables, sugar sweetened beverages and other foods) of the Aboriginal youth were sub-optimal when compared to current recommendations. This research project is unique because it comprehensively assessed adiposity, diet and physical activity, and the relationships between these variables, in a relatively large sample of Aboriginal boys and girls. Furthermore, these relationships were established using a variety of measures (i.e., DXA, waist circumference, height and weight) while controlling for biological confounders. Overall, the results highlight the urgent need to promote physical activity and healthy eating in Aboriginal youth and set the stage for future research.

BAKGRUND: Folsyra och järn är två viktiga ämnen som under graviditeten har olika betydelser dels för mamman och dels för fostret. Brister på dessa ämnen kan således leda till skador av olika slag och därför rekommenderas ofta kosttillskott till gravida kvinnor. Kvinnornas matvanor kan påverka om de får i sig tillräcklig mängd av dessa ämnen. SYFTE: Syftet med denna studie var att se hur gravida förhåller sig till att ha en hälsosam kost under graviditeten samt inställningen till kosttillskott med järn och folsyra. Studien syftade till att undersöka eventuella hinder samt se användningen av järn och folsyra. METOD: Studien är av kvantitativ typ med en enkät som inför denna undersökning har modifierats, Health and Taste Attitude Scale. Enkäten består av 7 frågor och 21 påståenden varav 6 st är från HTAS och resterande tillagda. Deltagarna kommer från ett internetforum "Min Bebis" där enkäten skickades ut som ett personligt meddelande till 151 st, varav 44 st kom att delta. En beskrivande analys gjordes med medelvärde och standardavvikelse. RESULTAT: Denna studie hade en majoritet av blivande förstagångsföräldrar och visade på att fler gravida kvinnor tar kosttillskott med järn än folsyra. Det verkar finnas en bristande konsumtion på livsmedel rika på folsyra och järn däribland blodpudding, frukter och fullkornsprodukter. I resultatet framkommer att de flesta inte har tagit tillskott av folsyra innan graviditet. De gravida verkar ha en måttlig inställning till att äta hälsosamt under sin graviditet. / BACKGROUND: Folic acid and iron are two important substances during pregnancy which have different meanings for the mother and the child. Deficiencies of these substances can lead to damage, and therefore often recommended supplements to pregnant. Women´s diet can affect whether they get enough of those substances. AIM: The aim of this study was to see how the pregnancy is related to having a healthy diet and attitudes to food supplements with iron and folic. The study aimed to investigate possible barriers and ensure the use of iron and folic acid. METHOD: This study is quantitative using a questionnaire developed for this study. The questionnaire contained part of the Health and Taste Attitude Scale. The items were partly modified to suit the aim of the study. The questionnaire was send as a message to 151 pregnant women in the internet forum "MinBebis" and 44 were participating. RESULTS: Respondents in this study were mainly prospective first-time parents and the results showed that more pregnant women take supplements with iron than folic acid. There seems to be a low consumption of foods rich in folic acid and iron, example fruit and whole grains. The majority did not take supplements of folic before pregnancy. The pregnant women seem to have a moderate approach to eating healthy during their pregnancy.

A rapid and accurate method to determine or predict cattle diet quality is essential to effectively manage free-ranging cattle production. One popular tool currently available for predicting cattle diet quality is fecal Near Infrared Reflectance Spectroscopy (NIRS) profiling, which requires considerable time and financial investment. Two approaches were taken to develop a replacement of NIRS fecal analysis for predicting real-time cattle diet quality. The first approach took advantage of a standing forage quantity monitoring and prediction model, and its animal diet selection sub model to model cattle diet quality. The second approach tested if a direct relationship is present between cattle diet quality and a simple temperature driven variable. The model used in the first approach is Phytomass Growth Model (PHYGROW). Using the Growing Degree Days (GDD) concept, forage crude protein estimation equations were developed. Coupled with PHYGROW diet selection sub model, cattle diet quality values were modeled. The validation study revealed good correlation between predicted diet quality and observed diet quality (r2=0.84). The Grazing Animal Nutrition lab (GAN lab) commercial fecal NIRS analyzing data for Major Land Resource Area 42 (MLRA 42) was used to analyze the relationship between GDD and cattle diet crude protein (CP). Repeatable high quality regressions were found for CP and GDD. A simple temperature based model was then developed to predict cattle diet quality for regional use. Another independent dataset for MLRA 116B from the GAN lab fecal NIRS data and a controlled grazing study were used to validate the relationship. The study showed that using GDD to predict cattle diet quality is a dependable tool, but regional specific relationships need to be developed. The two developed models set the foundation for remotely predicting cattle diet quality for effectively managing cattle production. The approaches also set the framework for developing broader applications for other animal species.

Equine gastric ulcer syndrome (EGUS) has been reported to occur in 40% to over 90% of horses across multiple equestrian disciplines. The objective of this study was to evaluate the effect of trace mineral supplementation on gastric ulcers in exercising yearling horses. Twenty-one Quarter Horse yearlings, 15 to 18 mo of age, were randomly assigned to 1 of 3 treatment groups. The control group received no supplemental trace minerals, the inorganic group received supplemental sulfated trace minerals, and the organic treatment received supplemental amino acid complexed trace minerals. The trial consisted of 3 consecutive 28-d periods, separated by 5 d of diet acclimation. The basal ration included a 14% CP textured concentrate, with coastal Bermudagrass hay fed in Periods 1 and 3 and alfalfa consumed in Period 2. In Periods 1 and 2, horses were exercised on a mechanical horse-exerciser, but were lunged or underwent training in an undergraduate course in Period 3. At the end of each period, horses were transported for 6 h, 5 d before being endoscopically examined to assign ulcer scores. Data were analyzed as repeated measures using the mixed procedure of SAS, with the model including fixed effects of treatment, period, and their interaction (SAS 9.2). All P-values < 0.05 were considered significantly different. Baseline EGUS scores were not different among treatment groups, with a mean of 2.1. There was no effect of treatment in any period, or a period by treatment interaction. There was a significant period effect (P < 0.01). Mean ulcer scores in Periods 1, 2, and 3 were significantly lower than baseline, with mean scores of 1.5, 1.0, and 1.5, respectively. There was a tendency (P=0.06) for mean ulcer scores to be lower in Period 2 than in Periods 1 and 3, which were not different. Average daily intakes of Zn, Mn, Cu, and Co were significantly higher in the organic and inorganic than control horses (P < 0.0001). Under the conditions of this study, trace mineral supplementation did not affect gastric ulcer appearance and severity. However, it is possible that dietary and management alternatives, such as hay and concentrate types, could be anti-ulcerogenic.

Abstract The present study investigated the activity patterns of Formosan macaques at Mt. Longevity and an emphasis was given to feeding and foraging behavior. The diet composition of Formosan macaque showed significant changes among different age and sex classes of macaques as well as among different seasons. The field research was carried out from August 2003 to July 2004 for a total of 311 hrs covering 77 days. Feeding behavior dominated the activity patterns of the macaques at Mt. Longevity (28.11%), followed by other behaviors such as affiliate (24.71%), resting (17.10%) and moving (16.04%). In contrast, foraging (8.22%) and agonistic behaviors (5.50%) were the least among the activity patterns recorded during this study. Interestingly, adult males spent more time in resting (30.60%) while adult females spent more time in feeding (29.84%), which indicated that the activity patterns were influenced by sex and age groups. Plant food items accounted for 94.87% of their diet while the seminal fluid and breast milk accounted for 5.08%. The plant food items consumed by the monkeys came from 46 species in 31 families and the fruits alone amounted 42.18%. Other food items included leaf (26.20%), stem (11.84%), flower (10.33%), bud (9.14%) and root (0.03%). The relative frequency of fruit consumption by the macaques was higher than 40 % between May and September (40.53% - 63.79%) and December to January (54.66% - 55.28%). The Formosan macaque diet composition changed according to the sex and age class groups. The Simpson index, Shannon-Wiener index and Levine¡¦s niche breadth index changed according to sex, age and months respectively. The plant food diversity indexes were highest for the juvenile males and lowest for the adult females. The Levine¡¦s niche breadth index was broader for the juvenile females but narrowest for the adult females. Adult females consumed 35 species of plants which is higher than the number of species that the adult males and juveniles consumed. However, the plant food diversity index and the niche breadth index were low for adult females, which indicate that they choose certain types of food. On the other hand, all these three indexes were highest in May and changed from month to month. The diet overlap of the Formosan macaque (Renkonen percentage) was highest between adult males and adult females (85.2%) than between other sex/age groups. Among the five categories of age and sex groups, the diet overlap was 66.68% and 22 species of plants was consumed by all age and sex groups of Formosan macaques.

The physical properties (i.e., mass, particle diameter and composition) of apolipoprotein B (apoB)-containing lipoproteins (apoB-LP) are a major determinant of atherosclerotic cardiovascular disease (ASCVD) risk. The objective of this research was to investigate how nascent apoB-LP physical properties affect circulating lipoprotein profiles and risk of disease. Relationships between apoB-LP physical properties and arterial plaque formation in four genotypes of mice with apoB isoform specific clearance defects were investigated. Multivariate statistical analysis found that arterial lesions were most closely related to genetic background and apoB concentration related to delayed clearance rate. For defining the dietary effects on circulating lipoprotein profiles, the physical properties of lipoproteins in hamsters fed high-carbohydrate diets containing either 60% fructose or 60% cornstarch for 2 wk were studied. Fructose increased very low-density lipoprotein (VLDL) particle diameter and decreased low-density lipoprotein (LDL) particle diameter. Elevations in all high-density lipoprotein (HDL) fractions were observed in the fructose-fed group. Further investigation was made of whether changes to the physical properties in circulating lipoproteins resulted from changes to nascent particles in the assembly and secretion processes. Intermediate particles used for lipoprotein assembly were isolated from rough endoplasmic reticulum of hamster liver, and nascent VLDL were isolated from plasma after Triton WR-1339 injection of hamsters. A large, TG-rich apoB-deficient particle and a small, lipid-poor apoB-containing particle were isolated in each dietary setting. The diameter of first-step particles was larger in fructose feeding, which indicated that apoB degradation decreases and provides the basis for apoB oversecretion. Fructose feeding significantly increased the concentrations recovered from liver for these two particles and for nascent particles compared with chow or starch feeding. Collectively, these results demonstrate: 1) genetic factors can dictate metabolism, and metabolic conditions can critically affect the physical properties and further atherogenicity of apoB-LP; 2) changes in physical properties of circulating apoB-LP are derived from changes to the nascent particles; and 3) dietary factors can influence the assembly, secretion, and metabolism of apoB-LP. The findings of the research may provide a metabolic basis for the recognition of new targets that could regulate apoB-LP metabolism to prevent and treat ASCVD.