Equine gastric ulcer syndrome (EGUS) has been reported to occur in 40% to over 90% of horses across multiple equestrian disciplines. The objective of this study was to evaluate the effect of trace mineral supplementation on gastric ulcers in exercising yearling horses. Twenty-one Quarter Horse yearlings, 15 to 18 mo of age, were randomly assigned to 1 of 3 treatment groups. The control group received no supplemental trace minerals, the inorganic group received supplemental sulfated trace minerals, and the organic treatment received supplemental amino acid complexed trace minerals. The trial consisted of 3 consecutive 28-d periods, separated by 5 d of diet acclimation. The basal ration included a 14% CP textured concentrate, with coastal Bermudagrass hay fed in Periods 1 and 3 and alfalfa consumed in Period 2. In Periods 1 and 2, horses were exercised on a mechanical horse-exerciser, but were lunged or underwent training in an undergraduate course in Period 3. At the end of each period, horses were transported for 6 h, 5 d before being endoscopically examined to assign ulcer scores. Data were analyzed as repeated measures using the mixed procedure of SAS, with the model including fixed effects of treatment, period, and their interaction (SAS 9.2). All P-values < 0.05 were considered significantly different. Baseline EGUS scores were not different among treatment groups, with a mean of 2.1. There was no effect of treatment in any period, or a period by treatment interaction. There was a significant period effect (P < 0.01). Mean ulcer scores in Periods 1, 2, and 3 were significantly lower than baseline, with mean scores of 1.5, 1.0, and 1.5, respectively. There was a tendency (P=0.06) for mean ulcer scores to be lower in Period 2 than in Periods 1 and 3, which were not different. Average daily intakes of Zn, Mn, Cu, and Co were significantly higher in the organic and inorganic than control horses (P < 0.0001). Under the conditions of this study, trace mineral supplementation did not affect gastric ulcer appearance and severity. However, it is possible that dietary and management alternatives, such as hay and concentrate types, could be anti-ulcerogenic.

Equine gastric ulcer syndrome (EGUS) is common amongst equine athletes of various disciplines and linked to decreased performance. Prevalence among racehorses has been reported to be over 90%, performance horses at 60%, and endurances horses at about 70%. In swine, concentrate form and smaller particle size increase gastric ulceration; thus, the objective of this study was to investigate the effect of concentrate type on EGUS. Quarter Horse yearlings (n=19; 12-18 mo) were blocked by initial EGUS score on a scale of 0 to 4 (0= no ulceration or hyperkeratosis, 4= extensive, deep ulceration) and sex, and utilized in a 77-d cross-over design with two 28-d periods separated by a 21-d washout period. During the first 28-d period, horses were separated into 1 of 2 treatment groups that were all fed Bermuda grass hay and either a commercially available pelleted or textured concentrate. After the initial 28-d period, horses were all fed pelleted feed and Bermuda grass hay for a 21-d washout period then treatment groups were switched for the final 28-d period. Baseline EGUS scores were not different between horses assigned to either treatment (mean 1.1); however, upon treatment, horses fed textured feed acquired a reduced incidence of ulceration as compared to those fed pelleted (mean score of 1.6 vs 1.1, respectively; P =0.02). Degree and incidence of ulceration was influenced by concentrate form; yearlings fed pelleted feed had higher ulcer scores then those fed textured feed. Therefore, the findings of this study suggests that textured feed may be a effective management tool to aid in the reduction of severity in horses afflicted with EGUS.

Submitted by Jean Medeiros (jeanletras@uepb.edu.br) on 2016-03-18T11:35:43Z No. of bitstreams: 1 PDF - Cynthia Layse Ferreira de Almeida.pdf: 3612014 bytes, checksum: ff23a88527b4e07c470c1b649ddb0716 (MD5) / Approved for entry into archive by Secta BC (secta.csu.bc@uepb.edu.br) on 2016-07-22T15:01:26Z (GMT) No. of bitstreams: 1 PDF - Cynthia Layse Ferreira de Almeida.pdf: 3612014 bytes, checksum: ff23a88527b4e07c470c1b649ddb0716 (MD5) / Approved for entry into archive by Secta BC (secta.csu.bc@uepb.edu.br) on 2016-07-22T15:01:35Z (GMT) No. of bitstreams: 1 PDF - Cynthia Layse Ferreira de Almeida.pdf: 3612014 bytes, checksum: ff23a88527b4e07c470c1b649ddb0716 (MD5) / Made available in DSpace on 2016-07-22T15:01:35Z (GMT). No. of bitstreams: 1 PDF - Cynthia Layse Ferreira de Almeida.pdf: 3612014 bytes, checksum: ff23a88527b4e07c470c1b649ddb0716 (MD5) Previous issue date: 2013-09-04 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Spondias purpurea L. (Anacardiaceae), popularly known as “siriguela”, is used in folk medicine for the relief of fever and pain, as diarrhea medication, antispasmodic, diuretic, anti-anemic and analgesic. Pharmacognostic studies with the species indicate the strong presence of tannins, flavonoids and triterpenes in their leaves, and roots, which are generally related to the antiulcer activity. The aim of this study was to evaluate the gastroprotective activity of the extract phase of S. purpurea in animal models. For this, the ethanolic extract (Sp-EtOHE) and the dichloromethane fraction (Sp-DCMF) of leaves of S. purpurea were obtained. Regarding the gastroprotective activity, doses of 125, 250 and 500 mg/kg (p.o.) were tested against different models of acute induction of ulcer by acidified ethanol, ethanol, stress immobilization and cold, and nonsteroidal anti-inflammatory drug (NSAID). In the model of acidified ethanol, there was a reduction in the ulcerative lesion index (ULI) for 61, 71 and 65% Sp-EtOHE and 29, 77 and 68% to Sp-DCMF. In ulceration caused by ethanol, Sp-EtOHE and Sp-DCMF in the same doses protect the gastric mucosa and were not altered parameters of the stomach contents of rats. The evaluation by the stress model, Sp-EtOHE and Sp-DCMF decreased the ULI in 23, 59 and 70% and 24, 61 and 72%, respectively. Previous models were made for the determination of C-Reactive Protein (CRP) in blood samples and were observed significant reductions of this parameter. Similarly, in the model of NSAID-induced gastric ulcers, there was inhibition of injuries to Sp-EtOHE and Sp-DCMF. In order to investigate the mechanisms of action related to gastroprotection promoted by SpEtOHE (250 mg/kg) and Sp-DCMF (250 mg/kg) was evaluated the involvement of nitric oxide and sulfhydryl compounds. Thus, it was verified that the gastroprotective effect of S. purpurea not involve the participation of nitric oxide. However, this effect is related to the participation of sulfhydryl compounds. Thus, these data suggest that S. purpurea presents gastroprotective activity, possibly related to mechanisms cytoprotectives. / Spondias purpurea L. (Anacardiaceae), popularmente conhecida como “siriguela”, é utilizada na medicina popular para o alívio de febre e dores, como antidiarréico, antiespasmódico, diurético, analgésico e antianêmico. Estudos farmacognósticos realizados com a espécie indicam a presença marcante de taninos, flavonoides e triterpenos em suas folhas, e raízes, os quais geralmente estão relacionados com a atividade antiulcerogênica. Assim, o objetivo deste trabalho foi avaliar a atividade gastroprotetora do extrato e fase de S. purpurea em modelos animais. Para isso foram obtidos o extrato etanólico (EEtOH-Sp) e a fase diclorometano (FaDCM-Sp) das folhas de S. purpurea. Com relação à atividade gastroprotetora, as doses de 125, 250 e 500 mg/kg (v.o.) do EEtOH e FaDCM foram testados frente a modelos de indução aguda de úlcera por etanol acidificado, etanol, estresse por imobilização e frio e anti-inflamatório não-esteroidal (AINE). No modelo de etanol acidificado, houve a redução do índice de lesão ulcerativo (ILU) em 61, 71 e 65% para EEtOH e 29, 77 e 68% para FaDCM. Nas ulcerações causadas por etanol, EEtOH-Sp e FaDCM-Sp nas mesmas doses protegeram a mucosa gástrica, bem como não foram alterados parâmetros do conteúdo estomacal dos ratos. Na avaliação pelo modelo do estresse, EEtOH-Sp e FaDCM-Sp diminuíram o ILU em 23, 59, 70 e 24, 61, 72%, respectivamente. Foram realizados para os modelos anteriores a dosagem da Proteína C reativa ultrassensível (PCR) nas amostras de sangue e foram observadas reduções significativas deste parâmetro. Da mesma maneira, no modelo de úlceras gástricas induzidas por AINE, houve inibição das lesões para EEtOH-Sp e FaDCM-Sp. No intuito de investigar os mecanismos de ação relacionados à gastroproteção promovida pelo EEtOH-Sp (250 mg/kg) e FaDCM-Sp (250 mg/kg), foi avaliado o envolvimento do óxido nítrico e dos grupamentos sulfidrílicos. Assim, foi observado que o efeito gastroprotetor de S. purpurea não envolve a participação do óxido nítrico. Entretanto, este efeito está relacionado à participação dos grupamentos sulfidrílicos, sugerindo que S. purpurea apresenta atividade gastroprotetora, possivelmente relacionada a mecanismos citoprotetores.

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal.

The objective of the study reported here was to document the distribution of epidermal growth factor receptor (EGFr) and quantitate receptor density in normal as well as ulcerated equine gastric squamous mucosa. Fifteen horses with endoscopically normal stomachs were divided into three equal groups. Group 1 was a normal control. A protocol that alternated 24 hour periods of free-choice hay with 24 hours of feed deprivation was utilized to induce squamous mucosal gastric ulceration in Group 2 (48 hours total off-feed) and Group 3 (96 hours total off-feed). Gastric tissue was collected from 3 stomach locations at post-mortem examination and an avidin-biotin immunoperoxidase technique was developed to stain the formalin-fixed tissue for EGFr. A computerized image analysis system was used to measure EGFr area and mean intensity values at four sites within the epithelium from the basal cell layers to the lumen in the ulcer/erosion margin, erosion bed, and 10-14 mm distant from the lesion. / Master of Science

Orientador: Joel Mesa Hormaza / Resumo: A úlcera péptica é provocada pelo desequilíbrio entre fatores lesivos e protetores das mucosas gástrica e duodenal, em consequência a fatores endógenos ou exógenos aos tecidos. Para seu tratamento, algumas plantas são popularmente empregadas e demonstram eficácia semelhante ou superior aos fármacos geralmente utilizados, ocasionando menores efeitos adversos. Apesar de várias pesquisas na área, a compreensão do mecanismo gastroprotetor dos compostos fitoterápicos ainda não é completa. Assim, com o objetivo de contribuir para a compreensão da ação farmacológica dos extratos de três plantas medicinais (Alchornea glandulosa, Davilla elliptica e Davilla nitida), foi realizada uma análise da distribuição elementar dos constituintes das amostras de mucosas gástricas de ratos tratados com os extratos vegetais e que tiveram úlceras gástricas induzidas por álcool ou por droga anti-inflamatória não-esteroidal (DAINE). O experimento foi realizado no Laboratório Nacional de Luz Síncrotron (LNLS) e permitiu a análise dos elementos Cl, Ca, Mn, Fe, Cu e Zn que contem relação com a formação ou neutralização das úlceras pépticas. De acordo com os mapas da distribuição dos elementos, foi possível detectar alta concentração de Fe, Cu e Zn no interior da lesão das amostras de úlcera induzida por DAINE, e de Cu, Zn e Cl no interior da lesão das amostras induzidas por álcool. Nas amostras de úlcera induzida por DAINE e tratadas com Alchornea glandulosa, as concentrações de Ca, Cu e Fe aumentaram. N... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The peptic ulcer is caused by the imbalance between harmful and protective factors of the gastric and duodenal mucosa, as a consequence of factors endogenous or exogenous to the tissues. For its treatment, some plants are popularly employed and demonstrate efficacy akin or superior to the drugs normally used, leading to less adverse effects. Although much research has been conducted in the field, the understanding of the gastroprotective mechanism of phytotherapic compounds is still not complete. Therefore, aiming to advance the understanding of the pharmacological action of the extracts of three medicinal plants (Alchornea glandulosa, Davilla elliptica e Davilla nitida), we conducted an analysis of the elemental distribution of constituents within samples of gastric mucosa from rats treated with phytoterapic extracts and affected with peptic ulcers induced by alcohol or nonsteroidal anti-inflammatory drugs (NSAIDs). The experiment was performed at the Brazilian Synchrotron Light Laboratory (LNLS) and allowed for the analysis of the elements Cl, Ca, Mn, Fe, Cu and Zn that are connected to peptic ulcers development or inhibition. According to the elemental distribution maps, it was possible to detect high concentrations of Fe, Cu and Zn inside the lesion from the NSAID-induced ulcer samples, and of Cu, Zn and Cl inside the lesion from the alcohol-induced ulcer samples. In NSAID-induced ulcer samples treated with Alchornea glandulosa, Ca, Cu and Fe concentrations were augmented... (Complete abstract click electronic access below) / Doutor

Submitted by FERNANDO CHRISTIANO GABRIEL MORELLI null (fcgmorelli@yahoo.com.br) on 2016-02-12T17:49:35Z No. of bitstreams: 1 Fernando - Tese versão final Repositório UNESP.pdf: 1900802 bytes, checksum: a8c9044a1f5a46e203d3432398fc0a07 (MD5) / Approved for entry into archive by Sandra Manzano de Almeida (smanzano@marilia.unesp.br) on 2016-02-15T11:32:07Z (GMT) No. of bitstreams: 1 morelli_fcg_dr_araca.pdf: 1900802 bytes, checksum: a8c9044a1f5a46e203d3432398fc0a07 (MD5) / Made available in DSpace on 2016-02-15T11:32:07Z (GMT). No. of bitstreams: 1 morelli_fcg_dr_araca.pdf: 1900802 bytes, checksum: a8c9044a1f5a46e203d3432398fc0a07 (MD5) Previous issue date: 2016-02-01 / Erosões e úlceras são achados comuns no abomaso e causam preocupação econômica nos mais variados sistemas de produção de gado. Muitos fatores podem predispor ao aparecimento de úlceras e acúmulo de gases no abomaso, incluindo alimentos grosseiros, estresse ambiental, deficiências de vitaminas e minerais e infecções bacterianas. Essas úlceras podem ser subclínicas, sendo descobertas nas necropsias ou após o abate do animal, ou levarem à redução da motilidade do órgão, prejudicando o fluxo do seu conteúdo e causando transtornos digestivos graves e até ao aparecimento de síndromes semelhantes à indigestão vagal. Existem informações a respeito da resposta do sistema imune na maior parte das mucosas do trato gastrintestinal de não-ruminantes e ruminantes, porém são raras a respeito do abomaso. Os objetivos desse estudo foram detectar os níveis de citocinas (IL-17A, IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2) no conteúdo abomasal em bovinos de corte, determinar o perfil Th1 ou Th2 dessas citocinas em animais com úlceras de grau 1 e 2 na região cárdica abomasal e comparar esses valores com os níveis de citocinas de animais sem úlceras (controle), em amostras colhidas em abatedouro, para auxiliar na compreensão da fisiopatologia do processo inflamatório local. A avaliação macroscópica e a classificação das úlceras foi realizada por meio de exames visual e histológico em amostras de tecidos da parede da região cárdica abomasal ulcerada. Os níveis de citocinas produzidas do líquido abomasal dos animais com ou sem úlceras foram avaliados por citometria de fluxo (método Cytometric Bead Array). As citocinas citadas foram detectadas no líquido do abomaso dos bovinos. Não houve diferença na liberação das citocinas entre os grupos com úlceras e o grupo sem úlcera, indicando um equilíbrio entre perfis Th1 e Th2 da resposta inflamatória. / Erosions and ulcers are common findings in the abomasum and cause economic concern in several livestock production systems. Many factors may predispose to ulcers and bloat in the abomasum, including roughage, environmental stress, deficiencies of vitamins and minerals and bacterial infections. These ulcers may be subclinical and are found during necropsy or after slaughter, or lead to reduction of abomasal motility, hindering the flow of your content and causing serious digestive disorders and even the appearance of syndromes similar to vagal indigestion. There are some studies evaluating the immune system response in most of the mucous membranes of the gastrointestinal tract of non-ruminants and ruminants, but rarely related to the abomasum. The aims of this study were to investigate the levels of cytokines (IL-17A, IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2) in the abomasal fluid of beef cattle, to determine the Th1 or Th2 profile of these cytokines in animals with types 1 or 2 ulcers located in the abomasal cardic region and to compare these levels with those of animals without ulcers (controls), in samples collected in an abbatoir, to help to the understand the pathophysiology of the local inflammatory process. Ulcers from the abomasal cardic region were macroscopicaly evaluated, then classified by histology. Cytokine levels in the abomasal fluid from animals with or without ulcers were evaluated by flow cytometry (Cytometric Bead Array). Cytokines were detected in the abomasum fluid of cattle. There was no difference in the release of cytokines between groups, indicating a balance between Th1 and Th2 profiles of the inflammatory response.

Submitted by Maike Costa (maiksebas@gmail.com) on 2017-09-11T12:49:46Z No. of bitstreams: 1 arquivototal.pdf: 2180300 bytes, checksum: e42cbf6e6ed01099835b19ef61a6b55b (MD5) / Made available in DSpace on 2017-09-11T12:49:46Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 2180300 bytes, checksum: e42cbf6e6ed01099835b19ef61a6b55b (MD5) Previous issue date: 2016-02-24 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Rosmarinic acid (RA) is a secondary metabolite present in several plant species, chemically characterized as a phenolic compound, derived from the esterification of caffeic acid and 3,4-dihydroxyphenyl lactic acid. Its name is derived from Rosmarinus officinalis, a species from which it was first isolated. Several biological effects have been described for RA as the antioxidant, antiallergic, anticancer, antimicrobial, neuroprotective, hepatoprotective, among others. The objective of the present study was to evaluate the acute toxicity, the gastroprotective activity of rosmarinic acid, and related mechanisms of action in animal models. In the acute toxicity model in female mice, rosmarinic acid at doses of 300 and 2000 mg / kg, v, did not show any behavioral changes in the parameters evaluated, nor did the changes in water and feed intake, body weight and macroscopic organ structure . Due to the presence of death at the dose of 2000 mg / kg, the LD50 of rosmarinic acid was set at 2500 mg / kg, according to OECD Guideline 423, which suggested low toxicity. The gastroprotective activity of RA was evaluated at different doses of 25, 50, 100 and 200 mg / kg (v0) in different models of acute ulcer induction: acidified ethanol, ethanol, immobilization and cold stress, non-steroidal anti-inflammatory NSAIDs) and contention of gastric juice. In the pharmacological screening with acidified ethanol in mice, RA and carbenoxolone decreased the ulcerative lesion index (ILU) in 42, 42, 40, 66 and 42%, respectively, when compared to the negative control group (saline solution 0.9 %). In the ethanol model, AR (50, 100 and 200 mg / kg) and carbenoxolone (100 mg / kg) reduced the ALU by 52, 68, 96 and 93%, respectively, when compared to the negative control group. In the stress model, RA (25, 50, 100 and 200 mg / kg) and cimetdine (100 mg / kg) decreased the ILU by 39, 41, 69, 71 and 40% when compared to the saline group 0 , 9%. In the NSAID-induced ulcer model RA (25, 50, 100 and 200 mg / kg) and cimetdine (100 mg / kg) decreased the ILU by 36, 39, 49, 67 and 29% when compared to the control group negative. In the gastric juice-induced ulcer model, RA (200 mg / kg) and cimetidine (100 mg / kg) when administered orally or intraduodenal reduced ILU by 38 and 51%; 43 and 31%, respectively, when compared to their negative controls. The presence of anti-secretory or neutralizing mechanisms (biochemical parameters), cytoprotection (sulfhydryl groups, nitric oxide, muco, prostaglandin), antioxidant (GSH) and immunoregulatory (TNF-, IL-1 and IL-10) were evaluated. It was observed that the gastroprotective effect of rosmarinic acid is not related to the alteration of the biochemical parameters of the gastric juice (pH, volume and [H +]), it does not involve nitric oxide, muco and prostaglandins, but it is related to the participation of the sulfhydryl groups , increased GSH levels, reduction of proinflammatory cytokines (IL-1 and TNF-) and maintenance of anti-inflammatory cytokines (IL-10) levels. In this way, it is possible to infer that rosmarinic acid has gastroprotective activity, related to cytoprotective, antioxidant and anti-inflammatory mechanisms. / O ácido rosmarínico (AR) é um metabólito secundário presente em diversas espécies de plantas, quimicamente caracterizado como um composto fenólico, oriundo da esterificação do ácido cafeico e do ácido lático 3,4 dihidroxifenil. Seu nome é derivado da Rosmarinus officinalis, espécie da qual foi isolada pela primeira vez. Diversos efeitos biológicos têm sido descritos para o AR como o antioxidante, antialérgico, anticâncer, antimicrobiano, neuroprotetor, hepatoprotetor, entre outros. O presente trabalho teve por objetivo avaliar a toxicidade aguda, a atividade gastroprotetora do ácido rosmarínico, e os mecanismos de ação relacionados, em modelos animais. No modelo de toxicidade aguda em camundongos fêmeas, o ácido rosmarínico nas doses de 300 e 2000 mg/kg, v.o, não demonstrou nenhuma alteração comportamental nos parâmetros avaliados, nem alterações no consumo de água e ração, peso corpóreo e na estrutura macroscópica dos órgãos. Devido a presença de morte na dose de 2000 mg/kg, a DL50 do ácido rosmarínico foi estipulada em 2500 mg/kg, de acordo com o guia 423 da OECD, o que sugeri baixa toxicidade. A atividade gastroprotetora do AR foi avaliada nas doses de 25, 50, 100 e 200 mg/kg (v.o) em diferentes modelos de indução aguda de úlcera: etanol acidificado, etanol, estresse por imobilização e frio, anti-inflamatório não-esteroidal (AINE) e contensão do suco gástrico. Na triagem farmacológica com o etanol acidificado em camundongos, o AR e a carbenoxolona diminuiu o índice de lesão ulcerativa (ILU) em 42, 42, 40, 66 e 42%, respectivamente, quando comparado ao grupo controle negativo (solução salina 0,9%). No modelo de etanol, AR (50, 100 e 200 mg/kg) e carbenoxolona (100 mg/kg) reduziu a ALU em 52, 68, 96 e 93%, respectivamente, quando comparado ao grupo controle negativo. No modelo de estresse, o AR (25, 50, 100 e 200 mg/kg) e a cimetdina (100 mg/kg) diminuiu o ILU em 39, 41, 69, 71 e 40%, quando comparado ao grupo solução salina 0,9%. No modelo de úlcera induzido por AINE o AR (25, 50, 100 e 200 mg/kg) e a cimetdina (100 mg/kg) diminuiu o ILU em 36, 39, 49, 67 e 29%, quando comparado ao grupo controle negativo. No modelo úlceras induzidas por contensão do suco gástrico, o AR (200 mg/kg) e cimetidina (100 mg/kg) quando administrado por via oral ou intraduodenal, reduziu o ILU em 38 e 51% ; 43 e 31%, respectivamente, quando comparados aos seus controles negativos. Foi avaliada a participação dos mecanismos antissecretórios ou neutralizante (parâmetros bioquímicos), citoproteção (grupamentos sulfidrila, óxido nítrico, muco, prostaglandina), antioxidante (GSH) e imunorregulatório (TNF-, IL-1 e IL-10). Foi observado que o efeito gastroprotetor do ácido rosmarínico não está relacionado à alteração dos parâmetros bioquímicos do suco gástrico (pH, volume e [H+]), não envolve a participação do óxido nítrico, muco e prostaglandinas, mas está relacionado a participação dos grupamentos sulfidrílicos, aumento dos níveis de GSH, redução de citocinas pró-inflamatórias (IL-1 e TNF-) e manutenção dos níveis de citocinas anti-inflamatórias (IL-10). Desta forma, é possível inferir que o ácido rosmarínico apresenta atividade gastroprotetora, relacionada a mecanismos citoprotetores, antioxidante e anti-inflamatórios.

Made available in DSpace on 2015-03-26T13:46:57Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1766831 bytes, checksum: 62438b176456ba87c3a689b13d0f0421 (MD5) Previous issue date: 2010-06-08 / The experiment took eight adult bitches, clinically healthy, mixed breed, weighing between 9 and 28 Kg. These animals formed two study groups at different times. At first the dogs comprised the control group and a moment later the same dogs formed the omeprazole group, with the aim of evaluating by endoscopy the period of healing of experimental gastric ulcer and effect of omeprazole in the treatment of this condition. The experiment was conducted at the Department of Veterinary, Federal University of Viçosa. The ulcerative gastropathy was induced by instilling 0.1 ml of sodium hydroxide at 40% through endoscopic visualization in the gastric mucosa in the pyloric antrum region. Dogs omeprazole group received 1 mg/kg of the drug orally for 24 in 24 hours during four weeks and the control group received placebo at the same moments. During the experiment, animals were assessed by daily physical examination, laboratory tests (CBC) before starting the experiment and at the end of the study, endoscopic examinations before the study began, and after induction of ulcerative gastropathy occur weekly until complete healing of lesions. The results showed that animals treated with omeprazole had better clinical and laboratory demonstrated endoscopic healing faster with a total recovery of the animals after 28 days, compared with 42 days required for full recovery of the control group. / Foram utilizadas 8 cadelas adultas, clinicamente sadias, sem raça definida, pesando entre 9 e 28 Kg. Estes animais formaram 2 grupos de estudo em momentos diferentes. No primeiro momento as cadelas compuseram grupo controle e num momento posterior as mesmas cadelas formaram o grupo omeprazol, com o objetivo de avaliar por endoscopia o período de cicatrização da mucosa gástrica ulcerada experimentalmente e o efeito do omeprazol no tratamento dessa condição. O experimento foi realizado no Departamento de Veterinária da Universidade Federal de Viçosa. A gastropatia ulcerativa foi induzida instilando-se 0,1 ml do hidróxido de sódio a 40% sob visualização endoscópica na mucosa gástrica em região de antro pilórico. Os cães do grupo omeprazol receberam 1 mg/kg do medicamento, via oral, de 24 em 24 horas, durante 4 semanas e os animais do grupo controle receberam placebo nos mesmos momentos. Durante o experimento, os animais foram avaliados por meio de exames clínicos diários; exames laboratoriais (hemograma) antes de se iniciar o experimento e ao término do estudo; exames endoscópicos antes do início do estudo, e após a indução da gastropatia ulcerativa semanalmente até ocorrer a completa cicatrização das lesões. Os resultados mostraram que os animais tratados com omeprazol tiveram melhor evolução clínica e laboratorial e demonstraram endoscopicamente uma cicatrização mais acelerada com total recuperação dos animais após 28 dias, em comparação aos 42 dias requeridos para total recuperação dos animais do grupo controle.