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Determining the role of protein regulators of hisactophilin on actin filament formationMcRorie, Paul Alexander 09 January 2013 (has links)
Protein structure and functions are tightly regulated. Studying the integration of multiple
modifications in single systems is a novel approach. Hisactophilin protein from Dictyostelium
discoideum, is an actin binding protein that serves to induce formation of actin filaments and is
regulated by protonation and myristoylation. Utilizing hisactophilin as a model, I determined the
effect of pH and myristoyl-switching on actin binding and filament induction using fluorescence
spectroscopy, light scattering, and time-course electron microscopy. Results revealed the
accessible myristoyl group slows binding and the rate of actin polymerization compared to when
the group is sequestered. Hisactophilin induces pH-dependent actin aggregates before
reorganizing them into filaments and bundles. Hisactophilin mutants impact initial actin binding
and the kinetics of the aggregated state. I determined the cooperativity of myristoylation and
protonation as interdependent protein regulatory mechanisms, their impact on actin binding and
proposed a novel mechanism for actin polymerization as a result of these integrated regulators. / NSERC
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Heterophilic Cell Adhesion Molecule TgrC1 and its Binding Partners during Dictyostelium discoideum DevelopmentChen, Gong 27 March 2014 (has links)
During development, Dictyostelium discoideum cells assume muticellularity via their collective aggregation. Cell-cell adhesion is required for morphogenesis, cell differentiation, cell sorting and gene expression during development. TgrC1 is a heterophilic cell adhesion molecule which is indispendable for complete development. TgrC1 can be considered as the most important cell adhesion molecule for D. discoideum development because deletion of the tgrC1 gene completely arrests development at the loose aggregate stage and inhibits fruiting body formation.
In order to investigate the biological role of TgrC1 during development, I have chosen to identify and charactize the extracellular heterophilic partner and the cytoplasmic binding partner(s) of TgrC1. Using different biochemical approaches, we identified TgrB1 as the heterophilic binding partner of TgrC1 and demonstrated that their association is mediated through IPT/TIG domains in the extracellular region of both proteins. Both tgrB1 and tgrC1 share the same transcriptional promoter and their spatiotemporal expression pattern is identical during development. We also examined the assembly of TgrC1-TgrB1 complexes via the split green fluorescence protein complementation assay and the fluorescence resonance energy transfer approach. Whereas TgrC1 is capable of forming cis-homodimers spontaneously, cis-homodimerization of TgrB1 depends on its trans-interaction with TgrC1. A model of the assembly process has been proposed.
To investigate signalling events initiated by the interaction between TgrB1 and TgrC1, pull-down assays were employed and led to the identification of myosin heavy chain kinase C as the cytoplamic partner of TgrC1. Mutational analysis showed that the basic residues in the short cytoplasmic domain of TgrC1 are critical to the binding with MHCK-C. Disruption of the interation between MHCK-C and TgrC1 results in an alteration of cell motility at the aggregation stage and aberrant cell sorting in slugs. These studies have highlighted the role of TgrB1-TgrC1 complexes in the regulation of morphogenesis during Dictyostelium development.
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On the function of the Dictyostelium Argonaute A protein (AgnA) in epigenetic gene regulationZhang, Xiaoxiao Unknown Date (has links)
Univ., Diss., 2006--Kassel
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Developmentally regulated non-coding RNAs in the social amoeba Dictyostelium discoideum /Hinas, Andrea, January 2007 (has links) (PDF)
Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2007. / Härtill 4 uppsatser.
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Regulation of glycogen phosphorylase genes in Dictyostelium discoideum /Sucic, Joseph F., January 1992 (has links)
Thesis (Ph. D.)--Virginia Polytechnic Institute and State University, 1992. / Vita. Abstract. Includes bibliographical references (leaves 106-114). Also available via the Internet.
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Spore germination in Dictyostelium discoideumCotter, David Allen, January 1965 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: l. 71-73.
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Domänenanalyse des Cyclase-assoziierten Proteins (CAP) und Charakterisierung von Filactin, einem neuartigen actinähnlichen Protein aus Dictyostelium discoideumIsrael, Lars. Unknown Date (has links) (PDF)
Universiẗat, Diss., 2003--München.
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Identification and partial characterization of a family of putative palmitoyltransferases in Dictyostelium discoideum /Wells, Brent Elliot, January 2003 (has links) (PDF)
Thesis (M.S.) in Biochemistry--University of Maine, 2003. / Includes vita. Includes bibliographical references (leaves 87-94).
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Factors influencing spore and cell size in Dictyostelium discoideumWeber, Allen Thomas, January 1967 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1967. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Dictyostelium als Wirtsmodell und Funktionsanalyse des Virulenzfaktors Mip aus Legionella pneumophilaWagner, Carina. January 1900 (has links) (PDF)
Würzburg, Univ., Diss., 2005. / Erscheinungsjahr an der Haupttitelstelle: 2004
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