In this thesis, I characterized the association of Arf4GDP with ER-Golgi intermediate compartment membranes. We confirmed that GDP-arrested Arf4 mutants associated with membranes irrespective of nature of tag or mutation. Recruitment appeared specific since loss of N-terminal myristoylation abolished binding. Surprisingly, mutations of residues unique to class II Arfs did not prevent recruitment of Arf4 to peripheral puncta. We then examined the failure of the GDP-arrested Arf4 mutant to disrupt Golgi structure. We identified residues R79 and E113 (likely involved in salt bridge interaction) only present in Arf1 and Arf5 as critical to the ability of their GDP-arrested mutants to disrupt Golgi structure. As predicted, introduction of these residues transformed Arf4GDP into a dominant negative mutant. Interestingly, overexpression of the putative ArfGDP receptor membrin prevented the effects of dominant negative Arf1 but not dominant negative Arf4. These results will facilitate identification of a novel Arf target critical to protein trafficking.

Diss. (sammanfattning) Linköping : Linköpings universitet, 2009. / Härtill 4 uppsatser.

The aim of this study was to explore language censorship in Zimbabwean films in Shona and English. The study concentrated on the themes of politics, culture and economic in the genre of the documentary, feature and short film genres. It was demonstrated that the Zimbabwean laws enabled authorities to impose censorship strategies that ranged from banning, restriction, persecution of filmmakers, withdrawal of films from circulation, and threats of withdrawal of permits of film retailers. These visible, direct and banal forms of censorship have forced some filmmakers to flee the country. Most of the filmmakers who have remained in the country have been forced to deal with themes that appear harmless to the state. This state induced form of self-censorship on the filmmakers has resulted in the production of uncritical, and unreflective films whose staple diet were embedded in cultural stereotypes. The study argued that language is a signifying practice that cannot be interpreted in a single direction. Thus, despite these realities of film censorship some filmmakers deliberately encoded or used verbal and visual film language that generated surplus meanings with which the films could be re-read in ways that reveal new linguistic strategies to evade and challenge both the restrictive censorship laws as well as criticise the undemocratic political culture that has taken root in Zimbabwe. The study used eclectic theories such as Marxism, audience-reception approach, critical legal theories and language theories to analyse the films. The explanatory capacity of these theories helped to reveal the contradictory ways in which the desire to impose restrictions on film meanings was constantly undermined in the innovative language of the films. / African Languages / D. Litt. et Phil. (African Languages)

The aim of this study was to explore language censorship in Zimbabwean films in Shona and English. The study concentrated on the themes of politics, culture and economic in the genre of the documentary, feature and short film genres. It was demonstrated that the Zimbabwean laws enabled authorities to impose censorship strategies that ranged from banning, restriction, persecution of filmmakers, withdrawal of films from circulation, and threats of withdrawal of permits of film retailers. These visible, direct and banal forms of censorship have forced some filmmakers to flee the country. Most of the filmmakers who have remained in the country have been forced to deal with themes that appear harmless to the state. This state induced form of self-censorship on the filmmakers has resulted in the production of uncritical, and unreflective films whose staple diet were embedded in cultural stereotypes. The study argued that language is a signifying practice that cannot be interpreted in a single direction. Thus, despite these realities of film censorship some filmmakers deliberately encoded or used verbal and visual film language that generated surplus meanings with which the films could be re-read in ways that reveal new linguistic strategies to evade and challenge both the restrictive censorship laws as well as criticise the undemocratic political culture that has taken root in Zimbabwe. The study used eclectic theories such as Marxism, audience-reception approach, critical legal theories and language theories to analyse the films. The explanatory capacity of these theories helped to reveal the contradictory ways in which the desire to impose restrictions on film meanings was constantly undermined in the innovative language of the films. / African Languages / D. Litt. et Phil. (African Languages)

Call number: LD2668 .T4 1979 R44 / Master of Science

Doctor of Philosophy / Department of Civil Engineering / Mustaque A. Hossain / Remaining service life (RSL) has been defined as the anticipated number of years that a pavement will be functionally and structurally acceptable with only routine maintenance. The Kansas Department of Transportation (KDOT) has a comprehensive pavement management system, network optimization system (NOS), which uses the RSL concept. In support of NOS, annual condition surveys are conducted on the state highway system. Currently KDOT uses an empirical equation to compute RSL of flexible pavements based on surface condition and deflection from the last sensor of a falling-weight deflectometer (FWD). Due to limited resources and large size, annual network-level structural data collection at the same rate as the project level is impractical. A rolling-wheel deflectometer (RWD), which measures surface deflections at highway speed, is an alternate and fast method of pavement-deflection testing for network-level data collection. Thus, a model that can calculate RSL in terms of FWD first sensor/center deflection (the only deflection measured by RWD) is desired for NOS. In this study, RWD deflection data was collected under an 18-kip axle load at highway speed on non-Interstate highways in northeast Kansas in July 2006. FWD deflection data, collected with a Dynatest 8000 FWD on the KDOT network from 1998 to 2006, were reduced to mile-long data to match the condition survey data collected annually for NOS. Normalized and temperature-corrected FWD and RWD center deflections and corresponding effective structural numbers (SNeff) were compared. A nonlinear regression procedure in Statistical Analysis Software (SAS) and Solver in Microsoft Excel were used to develop the models in this study. Results showed that FWD and RWD center deflections and corresponding SNeff are statistically similar. Temperature-correction factors have significant influence on these variables. FWD data analysis on the study sections showed that average structural condition of pavements of the KDOT non-Interstate network did not change significantly over the last four years. Thus, network-level deflection data can be collected at four-year intervals when there is no major structural improvement. Results also showed that sigmoimal relationship exists between RSL and center deflection. Sigmoidal RSL models have very good fits and can be used to predict RSL based on center deflection from FWD or RWD. Sigmoidal equivalent fatigue crack-models have also shown good fits, but with some scatter that can be attributed to the nature and quality of the data used to develop these models. Predicted and observed equivalent transverse-crack values do not match very well, though the difference in magnitude is insignificant for all practical purposes.

Research report presented to the Unisa School of Business Leadership / This study looks at five cases of organisational change and considers how the respondents have perceived the implementation of change within their organisations. / Graduate School of Business Leadership / MBA

The photograph depicts the remains of one of the many buildings destroyed in the fire in Chelsea, Mass. in 1908.

"Macaws is a field and laboratory guide to the identification of the Military Macaw and the Scarlet Macaw. Also included is a survey of all the Southwestern culture areas which have produced macaw remains...A "labor of love" by the author...Scholarly addition to our knowledge of Southwestern prehistory." --Southwestern Lore "Excellent monograph, well illustrated...Much useful and interesting data in this study."--American Antiquity

Post-mortem computed tomography (PMCT) is a non-invasive medical imaging technology that could be a valuable adjunct to traditional techniques in forensic practice. However, despite numerous theoretical advantages, integration of PMCT into forensic pathology, anthropology and odontology is currently restricted by the lack of scientific evidence. This thesis reviews the literature regarding the anthropological investigation of juvenile remains. The experimental chapters use PMCT images of the Scheuer Juvenile Skeletal collection, a unique collection of remains, that span the full age range of the developing human held in Dundee, and cases from the PMCT image archive at the East Midlands Forensic Pathology Unit. Images were acquired using multi-detector CT scanners and analysed using OsiriX three-dimensional imaging software. This thesis considers 1) if anthropological measurements are reproducible using PMCT, 2) if PMCT-derived measurements are accurate, compared with dry bone and orthopantomogram (OPT) examinations 3) what images and data are required to conduct a full anthropological examination to determine an individual’s biological profile using PMCT and finally 4) how to format and display these images appropriately to facilitate data sharing, international interpretation and future development of this method. These techniques were also used in the anthropological investigation of Richard III. Using age as the principle parameter, and assessment of both long bones and dentition, I have shown that 1) measurements used in the most frequently applied forensic anthropology techniques can be extracted from PMCT data, 2) PMCT measurements are accurate, and repeatable by multiple practitioners of various professional backgrounds and experience and 3) the information required to conduct a comprehensive anthropological examination can be condensed into a concise twopage ‘minimum data-set’ form. The results of this thesis provide new evidence to support the implementation of PMCT for anthropological examination in events requiring forensic investigation and disaster victim identification.