This thesis focuses on the design and creation of control unit for DC motors drives. A principle on which the DC motor is based, a method of its control and a possibility to obtain current information about the position are briefly described in this work. After that, the different types of buses for communication with a superior system and justification of the chosen one, are presented. In the next section, there is a special emphasis on creation of the suitable driver model and a design of the elecrical schematic and PCB. The end of the thesis contains a description of commission a PCB and testing the main functions.

This master’s thesis deals with coding information and QR codes generating. There is described operation principle of QR codes for this purpose, and design of the equipment. This device is able to encode the information received through the serial port and display it in the QR code on the OLED display. Microprocessor is part of the device, so there is outlined the procedure for programming and testing equipment.

Content of this work is oriented on mechanical testing of PWB. Deals with standards related to mechanical testing and quality evaluation of PWB. This works is engaged into industry standards, for example IPC or JEDEC. Studies principle and methods of chosen vibration tests. Further aim of this work is vibration fundamentals of PWB assembly. This work describes some research studies, which were conducted in past years and dealt with vibration stress issues. Shows individual factors, which has effect on vibration response of DPS and its destruction, like component position, acceleration difference between component and substrate, around temperature or material constants. In practical part was chosen method of vibration test and experiment in Labview programming interface was performed to verify these findings from vibration theory.

This thesis deals with the design and realization of SMD pick and place machine. Significant part of thesis was focused on computer vision and its application for SMD devices centering. Beside the mechanical construction the control software capable of use in production environment have been realized.

The first chapter will provide an introduction to the physiology, pathogenesis and biology of mycobacteria. Host-pathogen interactions, different modes of resistance of the bacteria, adaptations for survival under nutrient and oxygen depleted conditions has been discussed. This is followed by a general discussion on gene expression and regulation in the microbe. The physiology of bacteria under stresses from the view of the transcriptional regulation of specific genes has also been discussed. The scope and objective of the present study in M. smegmatis covered in the thesis has been considered at the end. The next chapter discusses the characterization of msdps promoter in vivo with the help of reporter gene assay technologies. With the advent of promoterless E. coli-mycobacterium shuttle vectors, activity assays can be easily performed to characterize unknown upstream putative promoter sequences of genes. Both the 1 kb upstream as well as a 200bp upstream region of msdps gene has been characterized by. Primer extension analysis and subsequent site directed mutagenesis studies reveal +1 transcription start site and the promoter consensus sequence for the msdps gene respectively. Next chapter comprises of the method of constructing heterologous in vitro transcription machinery in mycobacteria. It is followed by characterization of transcription initiation at two dps promoters of M. smegmatis. A novel pull-down assay has been designed which enabled us to identify the sigma factors in the reconstituted RNA polymerases to be associated with the respective dps promoters and to compare the regulation of the two genes at transcription level. Further characterization through single round in vitro transcription at mycobacterial promoters has been attempted. The following two chapters provide some newer insights into the structure-function relationship of the first Dps molecule, MsDps (MsDps1) with respect to its DNA binding activity. The DNA binding activity is associated with the higher oligomeric form only. With the help of time resolved anisotropy and Förster Resonance Energy Transfer (FRET) experiments, we have monitored the nature of Dps dodecamer-DNA complex and mapped the distance between the N and C169 position in the absence and the presence of DNA. A new computational programme, Maximum Entropy Method (MEM) has been applied successfully to analyze data obtained from phase-modulation (Phi-M) lifetime experiments in order to get distribution of lifetime. In the last chapter a new method is adopted to predict amino acids important for stabilizing the interface in a trimeric structure. Subsequently, single and double amino acid mutants of the native MsDps protein has been constructed through site directed mutagenesis and are scored for the ability of the mutants to oligomerize under conditions similar to that of the native protein. This helped us to propose a hypothetical model of the overall mechanism of the protein oligomerization process in solution.

As trends in broadband wireless communications applications demand faster development cycles, smaller sizes, lower costs, and ever increasing data rates, engineers continually seek new ways to harness evolving technology. The zero intermediate frequency receiver architecture has now become popular as it has both economic and size advantages over the traditional superheterodyne architecture.<p> Orthogonal Frequency Division Multiplexing (OFDM) is a popular multi-carrier modulation technique with the ability to provide high data rates over echo ladened channels. It has excellent robustness to impairments caused by multipath, which includes frequency selective fading. Unfortunately, OFDM is very sensitive to the carrier frequency offset (CFO) that is introduced by the downconversion process. The objective of this thesis is to develop and to analyze an algorithm for blind CFO recovery suitable for use with a practical zero-Intermediate Frequency (zero-IF) OFDM telecommunications system.<p> A blind CFO recovery algorithm based upon characteristics of the received signal's power spectrum is proposed. The algorithm's error performance is mathematically analyzed, and the theoretical results are verified with simulations. Simulation shows that the performance of the proposed algorithm agrees with the mathematical analysis.<p> A number of other CFO recovery techniques are compared to the proposed algorithm. The proposed algorithm performs well in comparison and does not suffer from many of the disadvantages of existing blind CFO recovery techniques. Most notably, its performance is not significantly degraded by noisy, frequency selective channels.

As trends in broadband wireless communications applications demand faster development cycles, smaller sizes, lower costs, and ever increasing data rates, engineers continually seek new ways to harness evolving technology. The zero intermediate frequency receiver architecture has now become popular as it has both economic and size advantages over the traditional superheterodyne architecture.<p> Orthogonal Frequency Division Multiplexing (OFDM) is a popular multi-carrier modulation technique with the ability to provide high data rates over echo ladened channels. It has excellent robustness to impairments caused by multipath, which includes frequency selective fading. Unfortunately, OFDM is very sensitive to the carrier frequency offset (CFO) that is introduced by the downconversion process. The objective of this thesis is to develop and to analyze an algorithm for blind CFO recovery suitable for use with a practical zero-Intermediate Frequency (zero-IF) OFDM telecommunications system.<p> A blind CFO recovery algorithm based upon characteristics of the received signal's power spectrum is proposed. The algorithm's error performance is mathematically analyzed, and the theoretical results are verified with simulations. Simulation shows that the performance of the proposed algorithm agrees with the mathematical analysis.<p> A number of other CFO recovery techniques are compared to the proposed algorithm. The proposed algorithm performs well in comparison and does not suffer from many of the disadvantages of existing blind CFO recovery techniques. Most notably, its performance is not significantly degraded by noisy, frequency selective channels.

Web cluster systems provide cost-effective solutions when scalable and reliable web services are required. However, as the number of servers in web cluster systems increase, web cluster systems incur long and unpredictable delays to manage servers. This study presents the efficient management schemes for web cluster systems. First of all, we propose an efficient request distribution scheme in web cluster systems. Distributor-based systems forward user requests to a balanced set of waiting servers in complete transparency to the users. The policy employed in forwarding requests from the frontend distributor to the backend servers plays an important role in the overall system performance. In this study, we present a proactive request distribution (ProRD) to provide an intelligent distribution at the distributor. Second, we propose the heuristic memory management schemes through a web prefetching scheme. For this study, we design a Double Prediction-by-Partial-Match Scheme (DPS) that can be adapted to the modern web frameworks. In addition, we present an Adaptive Rate Controller (ARC) to determine the prefetch rate depending on the memory status dynamically. For evaluating the prefetch gain in a server node, we implement an Apache module. Lastly, we design an adaptive web streaming system in wireless networks. The rapid growth of new wireless and mobile devices accessing the internet has contributed to a whole new level of heterogeneity in web streaming systems. Particularly, in-home networks have also increased in heterogeneity by using various devices such as laptops, cell phone and PDAs. In our study, a set-top box(STB) is the access pointer between the internet and a home network. We design an ActiveSTB which has a capability of buffering and quality adaptation based on the estimation for the available bandwidth in the wireless LAN.

This work deals with effects of nanoparticles, mainly copper and silver, on properities of lead-free solder pastes. It describes basic tests done on solder pastes according to IPC-TM-650 standards. In practical section this work focuses on the preparation of Sn-Bi solder paste and the measuring of viscosity. After that different concentrations of silver copper alloy nanoparticles are added to the SnBi solder paste. These solder pastes are tested by the IPC standarts and mechanical and resistive tests are done on printed circuit boards where the effects of mixed in nanoparticles are observed.

This thesis focuses on the design and implementation of the wireless localization module, using UWB technology with emphases on low-power firmwre based on RTOS. Wireless localization is based on TDoA algorithm.  The resulting HW module is designed as a four layer PCB, based on MCU crf52832 (ARM Cortex M4) and UWB module DevaWave DW1000. Firmware is implemented using FreeRTOS with emphasis on low power consumption. For hardware implementation, Eagle CAD was used. Firmware is implemented in C and Assembly programming languages.