M.Tech. (Chiropractic) / A myofascial trigger point is a hyperirritable point within a tight band of voluntary skeletal muscle. The condition causes levels of mild discomfort to intense pain to patients that usually results in loss of man hours and compulsory pain medication. Dry needling is the treatment of choice and other techniques are seldom considered. Dry needling therapy (DNT) is an effective tool in the chiropractic profession but comes with significant drawbacks, such as patients whom have needling phobias and patients who often experience post needling soreness are challenging to treat with DNT. Further and more serious risks include pneumothorax when needling the muscles over the lung fields, which also limits the treatment scope of DNT. Low level laser therapy (LLLT) is non-invasive and non-threatening to patients and could serve as an alternative to DNT. This study therefore aimed, to determine if LLLT could be an alternative treatment to DNT by comparing DNT to LLLT on a target group that all have active trapezius trigger point two myofascial trigger points. The group consisted of 40 participants with posterior trapezius myofascial neck pain caused by active myofascial trapezius trigger points. Participants were then randomly divided into two groups. Group A (20 participants) would receive DNT to the active myofascialtrapezius trigger point two (TP2) and group B (20 participants) would receive LLLT also to the active myofascial trapezius TP2. Participants would then be treated according to a set protocol, over a two week period with a total of four treatments. Subjective and objective readings were taken and noted on the first, third and fifth visits. Subjective data was collected from the visual analogue scale and the Vernon-Minor neck pain and disability index questionnaires. Whereas the cervical range of motion (CROM) and algometer readings provided the objective data.

M.Tech. / This study was conducted to determine the effect of Sacroiliac joint adjustment to the restricted Sacroiliac joint in combination with myofascial dry needling to the active/latent Rectus Femoris muscle trigger point and Rectus Femoris myofascial dry needling on its own on Quadriceps Femoris muscle strength. Thirty participants between the ages of 18 and 40 years were recruited through the use of advertisements placed in and around the University of Johannesburg’s Chiropractic Day Clinic. Participants were assessed for exclusion criteria by performing a Full Case History, Pertinent Physical Examination, Lumbar Spine and Pelvis Regional Examination and S.O.A.P note. The participants who conformed to the specific inclusion criteria were accepted for this study. These participants were randomly placed into two groups of sixteen participants each. Group One receive Sacroiliac joint adjustment to the restricted Sacroiliac joint and ipsilateral myofascial dry needling of the Rectus Femoris muscle active/latent myofascial trigger points. Group two received myofascial dry needling of the Rectus Femoris muscle active/latent myofascial trigger point on the same side as the Sacroiliac joint restriction. Each participant received five treatments over a three week period, i.e. two treatments for the first two weeks and one follow-up treatment in the third week. The subjective data of the patients lower back pain was assessed using the Numerical Pain Rating Scale. The objective data was obtained from the Isometric Dynamometer measuring the Quadriceps Femoris muscle strength before and after each treatment. In addition the Algometer was used for measuring the pressure pain threshold of the Rectus Femoris trigger points. An analysis was performed using Repeated Measures Analysis, t-Test, Frequencis, Descriptives and Friedman’s Tests. The results of this study demonstrate that Sacroiliac joint adjustment to a restricted Sacroiliac joint with ipsilateral myofascial dry needling of the Rectus Femoris muscle showed a statistically significant, but temporary increase in Quadriceps Femoris muscle strength. Myofascial dry needling of the Rectus Femoris muscle on its own was found to be statistically insignificant for the increase in Quadriceps Femoris muscle strength.

M.Tech. (Chiropractic) / Neck pain is a common and costly complaint in society and many are made to believe that their neck pain is caused by pinched nerve, compressed disk, arthritis or displaced cervical vertebrae, when in reality the pain may be solely due to referral from myofascial trigger points in overworked or traumatized muscles of their upper back and shoulders. Travel and Simons (1999) demonstrated trapezius muscle of the neck, back and shoulder as the main cause of mechanical neck pain and stiffness. The aim of this study was to compare the effects of trigger point therapy using an activator instrument versus myofascial dry needling in combination with cervical spine adjustment in the treatment of those with acute or chronic neck pain associated with active trigger point 1 (TrP 1) or trigger point 2 (TrP 2) of upper trapezius muscle, with regards to pain and disability, pressure pain threshold and cervical spine range of motion. The clinical study consisted of forty participants, from the ages of 18 and 45, randomly allocated into two groups of twenty individuals each. Potential participants were examined and accepted based on inclusion and exclusion criteria. Group 1 received activator trigger point therapy to upper trapezius TrP 1 or 2 with chiropractic adjustment to restricted segments of the cervical spine, and group 2 received myofascial dry needling of upper trapezius TrP 1 or 2 with chiropractic adjustment to restricted segments of the cervical spine. Participants were treated four times over a period of two weeks. Subjective data was collected using the Vernon-Mior Neck Pain and Disability Index and a Visual Analog Scale. Objective data was collected using an algometer to measure pressure pain threshold of trapezius TrPs muscles, and a goniometer to measure cervical spine range of motion. All data was collected at the first and third visits prior to treatment, and at the fifth visit. The statistical analysis was conducted using nonparametric tests. Friedman’s test was used to assess whether neck pain, disability, cervical spine range of motion and pressure pain threshold varied over the three time intervals. Wilcoxon Signed Ranks Pair test was used for assessment of comparability of the results in each group separately, and the Mann-Whitney U test was used for comparison of the accumulated data in the two groups.

M.Tech. (Chiropractic) / Objective: The purpose of this study was to compare dry needling with cervical spine manipulation combined with dry needling, in the treatment of infraspinatus muscle myofascial trigger points. Study Design: This was a randomized experimental study Setting: The participants were treated at the University of Johannesburg chiropractic day clinic Subjects: 30 participants volunteered for the study. The participants were divided into groups by randomly drawing thirty numbers from a hat, and placing them into two groups: either dry needling combined with spinal manipulation (group 1) or dry needling only (group 2). Participants had to be between eighteen and fifty years old and they could have been male or female. Participants had to present with active infraspinatus muscle myofascial trigger points and also had to present with shoulder or lateral arm pain reproduced by infraspinatus trigger point palpation. Method: Participants were either treated using dry needling only or dry needling combined with spinal manipulation. There were seven consultations. The first six consultations were treatment consultations. Measurements were taken at consultations one, four and seven. Results: The results indicate statistically that participants who had dry needling on its own had a faster increased pressure pain tolerance and a faster decrease in resting electrical activity of the muscle over the treatment period than those that recieved dry needling in combination with spinal manipulation. We must emphasize however that this did not show in the numerical pain rating scale. Participants felt a relatively even relief of their pain in both groups. These overall results might have been skewed by outliers in groups and a small demographic study size. The rest of the results were statistically insignificant, however there was a clinically desired therapeutic effect noted in both groups throughout the study. Conclusion: Dry needling only and dry needling combined with spinal manipulation, individually have desired therapeutic effects in the treatment of infraspinatus myofascial trigger points. There is however no conclusive statistical results in this study that shows one treatment protocol to have a greater therapeutic effect when compared to the other. However this study size was small and therefore the results may have been coincidental.

Magister Scientiae Dentium - MSc(Dent) / The purpose of this study is to evaluate the need for prophylactic antibiotic treatment in third molar surgery and to establish specific guidelines for antibiotic prophylaxis in the department of Maxillo-Facial and Oral Surgery (MFOS) at Tygerberg Academic, Groote Schuur and Mitchells Plain Hospitals.

Please read the abstract in the section, 00front of this document / Dissertation (M Inst Agrar (Land-Use Planning))--University of Pretoria, 2008. / Agricultural Economics, Extension and Rural Development / MInst Agrar / Unrestricted

La sécheresse oculaire est une pathologie fréquente qui peut impacter fortement la qualité de vie des patients. D’origine multifactorielle complexe, elle présente de nombreuses étiologies et son diagnostic est difficile en raison de présentations cliniques variées, en termes de symptômes et de tests cliniques, et en l'absence d'un marqueur spécifique. En outre, elle ne bénéficie actuellement que d’une seule molécule thérapeutique, cependant non curative, la ciclosporine. Devenant un réel problème de santé publique, la recherche visant à une meilleure compréhension des mécanismes sous-jacents à son apparition reste indispensable. L'état actuel des connaissances sur la sécheresse oculaire reconnaît à l'inflammation et à l'hyperosmolarité un rôle central. Ce travail de thèse a eu pour objectif d'étudier le comportement pro-inflammatoire des cellules conjonctivales dans un modèle in vitro classique de sécheresse oculaire induite par une hyperosmolarité saline. Nous avons ainsi montré que les cellules conjonctivales sécrètent une chimiokine pro-inflammatoire majeure, CCL2/MCP-1, et que cette induction est totalement sous la dépendance du facteur de transcription osmoprotecteur NFAT5/TonEBP ainsi que partiellement liée à la voie des MAPKs et de NFκB. Nous avons également observé que cette induction était en partie inhibée par la ciclosporine et la dexaméthasone. Nous avons ensuite complété ce travail par l'étude de l'inflammasome à la fois sur ces cellules et sur des monocytes, type cellulaire majeur ciblé par CCL2, en utilisant différents activateurs classiques dans le modèle d’hyperosmolarité. Nos résultats ont montré l’absence d’activation de l’inflammasome par l'hyperosmolarité seule. En revanche, l'hyperosmolarité aurait un rôle inhibiteur de l’inflammasome dans les monocytes. Ces résultats ouvrent des pistes nouvelles d’exploration afin de mieux comprendre les relations entre monocytes et cellules épithéliales de la conjonctive. Ils nous ont permis d'identifier le rôle incontournable de cellules épithéliales conjonctivales dans l'apparition ou le maintien de l'inflammation dans la pathologie et de caractériser certaines voies intracellulaires impliquées dans ce processus, ouvrant de nouvelles perspectives en termes de thérapeutiques. / Dry eye disease is a common condition that significantly impacts the quality of life of patients. It is a complex multifactorial disease with many etiologies and a diagnosis recognized as difficult due to the multiple clinical presentations in terms of symptoms and clinical tests, and lacking of a reliable and specific marker. There is currently only one therapeutic molecule to treat this disease, cyclosporin. However it is not curative. Dry eye disease is becoming an important public health problem and it is necessary to focus research on its underlying mechanisms, as they remain largely unknown. The current state of knowledge in dry eye disease recognizes inflammation and hyperosmolarity as central elements in the pathology. This work aimed to study the pro-inflammatory behavior of conjunctival cells in an in vitro model of dry eye disease by NaCl-induced hyperosmolarity. We showed that conjunctival cells secrete a major pro-inflammatory chemokine known as CCL2/MCP-1. This induction depends entirely on the osmoprotectant transcription factor NFAT5/TonEBP and is partially related to the MAPKs and the NFκB pathways. We showed that the process could be partially inhibited by cyclosporin and dexamethasone. We also studied the inflammasome in conjunctival cells and monocytes, the major cell type targeted by CCL2, using various classical inflammasome activators in this hyperosmolarity model. We did not observe any inflammasome activation induced by hyperosmolarity alone. On the other hand, hyperosmolarity shows signs of inhibiting inflammasome activation in monocytes. These results open new ways of understanding the relations between monocytes and conjunctival epithelial cells. They highlight the crucial role of the conjunctival epithelial cell type in the inflammation process in dry eye disease and make it possible to characterize intracellular pathways involved in this process, opening new therapeutic prospects.

Lithium-ion batteries (LIBs) have been widely used as the most popular rechargeable energy storage and power sources in today’s portable electronics, electric vehicles, and plug-in hybrid electric vehicles. LIBs have gained much interest worldwide in the last three decades because of their high energy density, voltage, rate of charge and discharge, reliability, and design flexibility. I am exploring the possibility of developing battery manufacturing technologies that would lower the cost, reduce the environmental impact, and increase cell performance and durability. This dissertation is focused firstly on understanding the effect of mixing sequence (the order of introducing materials) and optimizing the electrode fabrication for the best electrochemical performance, durability, lower cost, and improve the existing manufacturing processes. The electrode system consists of active material, polymer binder, conductive agent, and solvent. I have investigated four different mixing sequences to prepare the slurries for making the positive electrode. The key sequence-related factor appears to be whether the active material and conductive agent are mixed in the presence of or prior to the introduction of the binder solution. The mixing sequences 1, 2, 3, and 4 were optimized, and the rheological behavior of the slurries, morphology, conductivity, and mechanical and electrochemical properties of electrodes were investigated. Slurries from sequences 1 and 4 show different rheological properties from 2 and 3. The amount of NMP required to achieve a comparable final slurry viscosity differed significantly for the sequences under study. The sequence 1 shows better long-term cycling behavior than sequences 2, 3 and 4. This study quantifies the link between electrode slurry mix parameters and electrode quality. Secondly, a new method of making lithium-ion battery electrodes by adapting an immersion precipitation (IP) technology commonly used in membrane manufacturing was developed and demonstrated. The composition, structure, and electrochemical performance of the electrode made by the IP method were compared favorably with that made by the conventional method. The toxic and expensive organic solvent (NMP) was captured in coagulation bath instead of being released to the atmosphere. The IP electrodes show an excellent performance and durability at potentially lower cost and less environmental impact. Thirdly, I have developed and demonstrated a solvent-free dry-powder coating process for making LiNi1/3Mn1/3Co1/3O2 (NMC) positive electrodes in lithium-ion batteries, and compared the performance and durability of electrodes made by the dry-powder coating processes with that by wet-slurry coating processes. The technology that has been used is the electrostatic spray deposition (ESD) process. This process eliminates volatile organic compound emission, reduces thermal curing time from hours to minutes, and offers high deposition rates onto large surfaces. The long-term cycling shows that the dry-powder coated electrodes have similar performance and durability as the conventional wet-slurry made electrodes.

Soil mixing is a procedure that has proven to be effective for loose or soft compressible soils. The method stabilizes the soil in-place using specialized augers, tillers, or paddles that inject grout or dry cementitious powders as part of the mixing process. The Federal Highway Administration design manual for soil mixing helps to estimate the required amount of cementitious binder to produce a target design strength. However, it is biased towards inorganic soils and only mentions caution when confronting organic soils which usually come with a high water table, moisture content and void volume. The Swedish Deep Stabilization Research Centre cited studies with highly organic soils in regards to soil mixing and suggested that organic soils may need to reach a ‘threshold’ of cement content before strength gain can occur. The University of South Florida also conducted a study on highly organic soils and was able to confirm this concept. USF also proposed a threshold selection curve based on the organic content. This thesis extends this concept to the bench scale testing of multiple full scale field studies. This thesis will conclude with the presentation of new threshold curves based on the new data from the added field case studies. Given that there were variable binders and soil types used in the data analyzed, these threshold curves are dependent upon soil type and binder type, thus expanding upon the curve previously suggested.

This study aims to quantify ground ice content and describe the cryostructures and sediment in 15 ice-bearing permafrost cores collected from nine sand-wedge polygons in University Valley. The objectives were reached through laboratory measurements and computed tomodensitometric (CT) scanned image analysis of the permafrost cores. The soils in the valley were predominantly medium-sand. Four types of cryostructures were present in the cores: structureless, suspended, crustal and porphyritic. Excess ice content ranged from 0 to 93%, gravimetric water content ranged from 13 to 1881% and volumetric ice content varied from 28 to 93%. Median excess ice, volumetric ice and gravimetric water contents significantly increased in the top 20 cm of the cores taken from the polygon shoulders with increasing distance from University Glacier. Ground ice was preferentially stored in the centre of the investigated polygons where the ground surface remains cryotic throughout the year. Conversely, higher ground ice contents were measured in the shoulders of the investigated polygon where the ground surface is seasonally non-cryotic. CT-scanned images were shown to reasonably assess the distribution and presence of excess ice in permafrost cores taken from a cold and hyper-arid environment. The results of this thesis provide the first cryostratigraphic study in the McMurdo Dry Valleys of Antarctica.