Submitted by Arthur Arrobas Martins Barroso null (arthuragro07@hotmail.com) on 2017-09-06T14:00:26Z No. of bitstreams: 1 Tese_Arthur_Arrobas_Martins _Barroso.pdf: 1551513 bytes, checksum: 9d065640a6416beb7443eb7e6ad2dd94 (MD5) / Approved for entry into archive by Luiz Galeffi (luizgaleffi@gmail.com) on 2017-09-06T16:52:44Z (GMT) No. of bitstreams: 1 barroso_aam_dr_jabo.pdf: 1551513 bytes, checksum: 9d065640a6416beb7443eb7e6ad2dd94 (MD5) / Made available in DSpace on 2017-09-06T16:52:44Z (GMT). No. of bitstreams: 1 barroso_aam_dr_jabo.pdf: 1551513 bytes, checksum: 9d065640a6416beb7443eb7e6ad2dd94 (MD5) Previous issue date: 2017-07-18 / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / As culturas agrícolas estão sujeitas a conviver com plantas daninhas que podem, em determinadas situações, reduzir seu potencial genético de produção, causando prejuízos. Na maioria das vezes, devido à praticidade e ao custo, essas plantas são controladas pela aplicação de herbicidas, o que se denomina de controle químico. Dentre os produtos utilizados, está o glyphosate, que nos últimos anos vem sendo usado de maneira repetitiva devido à presença quase que exclusiva de culturas tolerantes a esse herbicida, como a soja, o algodão e o milho. Com isso, a utilização desse herbicida vem selecionando, nos últimos anos, plantas que apresentam adaptações para resistir a sua ação, dentre elas o azevém e o capim-pé-de-galinha. A resistência pode ser causada por diferentes mecanismos, envolvendo ou não a enzima-alvo de atuação do herbicida. Para o glyphosate, essa enzima é a 5-enolpiruvilshiquimato-3-fosfato, e essa pode apresentar mutações simples ou duplas. Essas mutações, além de afetar a tolerância da planta ao herbicida, podem modificar a fisiologia e o metabolismo da espécie, tornando-a mais ou menos adaptada ecologicamente, o que é denominado de fitness. Este trabalho teve por objetivo estudar os impactos da resistência ao glyphosate nas duas espécies supracitadas. Em um primeiro trabalho, plantas de azevém resistentes ao glyphosate foram comparadas a plantas suscetíveis quanto a seu perfil metabólico e proteico antes e após a aplicação do herbicida. As plantas suscetíveis apresentaram maiores níveis de aminoácidos produzidos derivado da rota do ácido chiquímico e menores teores de glyphosate em suas folhas, 72 horas após a aplicação do herbicida. Observou-se que as plantas suscetíveis apresentaram maior desenvolvimento, maior expressão de proteínas ligadas ao sistema fotossintético do azevém e expressão diferencial de proteínas ligadas à defesa vegetal contra estresses, ausentes nas plantas resistentes. Após a aplicação do herbicida, as plantas suscetíveis morreram, e as resistentes sobreviveram, passando a expressar, também, a enzima EPSPS sintase, sendo esse um dos mecanismos de resistência encontrados para a espécie. Em um segundo trabalho, avaliaram-se, em dois experimentos, os impactos da resistência ao glyphosate, causados por mutações simples ou duplas, no capim-pé-de-galinha, e seus efeitos na cultura da soja. O desenvolvimento e a fecundidade do capim-pé-de-galinha são pouco afetados pela mutação simples na posição 106 da enzima EPSPS, na ausência do glyphosate. Por outro lado, a mutação dupla da enzima nas posições 102 e 106 gera elevados custos no desenvolvimento e na reprodução das plantas. Quando se aplica o herbicida, a situação inverte-se. Plantas com a presença de uma mutação passam a sofrer intoxicação com o herbicida, chegando, inclusive, a morrer, enquanto se observa sobrevivência total de plantas com duplas mutações. Quando em convivência com a soja, na ausência do herbicida, tem-se a cultura mais afetada pela convivência com os genótipos suscetíveis e com uma única mutação. Na presença do herbicida, nas condições observadas, a interferência das plantas de capim-pé-de-galinha foi reduzida. / Crops are subject to live with spontaneous plants that may in certain situations reduce their genetic potential of production, causing losses. Most of the time, due to the practicality and cost, these plants are controlled by the application of herbicides, what is called chemical control. Among the products for this control, there is glyphosate, which in recent years has been used repetitively due to the almost exclusive presence of crops tolerant to this herbicide, such as soybean, cotton and corn. The use of this herbicide has been selecting, therefore in the last years plants that present adaptations to resist its application, among them Italian ryegrass and goosegrass. The resistance can be caused by different mechanisms, involving or not the target enzyme of action of the herbicide. For glyphosate, this enzyme is 5-enolpyruvyl-silicon-3-phosphate and it may present single or double mutations. These mutations, in addition to affecting the tolerance of the plant to the herbicide, can modify the physiology and metabolism of the species, making it more or less ecologically adapted, which is called fitness. The objective of this work was to study the impacts of glyphosate resistance on the two species mentioned above. In a first work, glyphosate resistant Italian ryegrass plants were compared to susceptible plants for their metabolic and protein profile before and after herbicide application. Susceptible plants showed higher levels of amino acids produced from the shikimic acid route and lower levels of glyphosate in their leaves 72 hours after the application of the herbicide. It was observed that the susceptible plants presented greater development, proteins linked to the greater ryegrass physiology expressed and differential expression of proteins bound to vegetal defense against stresses, absent in resistant plants. After the application of the herbicide, the susceptible plants died, and the resistant ones continued their normal physiology and start expressing the EPSPS synthase enzyme, being this one of the mechanisms of resistance found for the species. In a second work, the impacts of resistance to glyphosate, caused by single or double mutations in goosegrass and its effects on soybean crop, were evaluated in two experiments. The development and fecundity of goosegrass is little affected by simple mutation at position 106 of the EPSPS enzyme in the absence of glyphosate. On the other hand, the double mutation of the enzyme at positions 102 and 106, generates high costs in the development and reproduction of plants. When the herbicide is applied, the situation reverses. Plants with the presence of a mutation begin to present phytointoxication with the herbicide. When living with soybeans, in the absence of the herbicide, the culture is most affected by the coexistence with the susceptible genotypes and genotype with a single mutation. In the presence of the herbicide, under the conditions observed, the interference of goosegrass plants was reduced. / CNPq: 140943/2014-5

Thesis (M.Sc. Agriculture (Pasture Science)) -- University of Limpopo, 2021 / Finger millet (Eleusine coracana) is believed to be adapted to the arid and semi-arid regions, highly tolerant to pests, diseases and drought. It has the potential to produce a high forage biomass with fewer inputs under good production practices. The aim of the study was to evaluate the potential of finger millet as a fodder crop on the Pietersburg Plateau under rainfed and irrigation conditions, planted in rows and broadcast. The study was conducted for two consecutive seasons (2017 and 2018) at the Syferkuil Experimental Farm (SEF), University of Limpopo. Treatments consisted of two watering treatments (irrigation and rainfed) and two planting methods (broadcast and row planting). Seeding rate was 10 kg ha-1 with the inter row spacing of 25 cm. Irrigation had a significant effect on the dry matter production of finger millet (P ≤ 0.05). During 2017 growing season, under rainfed condition, the crop experienced zero production due to low rainfall. The total dry matter production of finger millet under rainfed conditions in 2018 was 3371 kg ha-1 for row planting and 3770 kg ha-1 for broadcasting. The dry matter production of finger millet under irrigation and row planting was 5318 kg ha-1 compared to 3371 kg ha-1 produced under row planting in the rainfed conditions. Broadcasting under irrigation produced 4890 kg ha-1 whereas broadcasting under rainfed conditions yielded 3770 kg ha-1. Planting method had no significant effect on the dry matter production of finger millet (P ≤ 0.05). The total dry matter production in 2017 was 5668 kg ha-1 and 5122 kg ha 1 under row planting and broadcast respectively, 2018 season produced the total dry matter production of 5122 kg ha-1 under row planting and 4892 kg ha-1 under broadcast. Finger millet planted under rainfed in rows had the CP% of 14.76 and 16.87% when broadcasted. In all the treatments CP% was higher than 10%. The ADF% was 33.02% under rainfed conditions and it ranged between 30.99% and 31.53% in 2017 and 2018 for row planting under irrigation. Finger millet can be considered an alternative fodder crop for livestock farmers in the Pietersburg Plateau

Despite an increase of biotechnical studies in third world countries, the bioinformatical side is largely lacking. In this paper we attempt to further the bioinformatical capabilities of east Af-rica. The project consisted of two teaching segments for east African doctorates, one as part of an academic workshop at ILRI, Kenya, and one in a small class at SLU, Sweden. The project also included the generation of two simple to use bioinformatical pipelines with the explicit aim to be reused by novice bioinformaticians from the very same region. The viability of the piplines were verified by generating transcriptional expression level differences for Paeni-bacillus polymyxa strain A26 and whole genome annotations for Setaria digitata. Both pipe-lines may have some merit for the collaborative effort between ILRI and SLU to annotate Eleusine coracana, a draught resilient crop, the annotation of which may save lives. The teaching material, source code for the pipelines and overall teaching impression have been included in this paper.

Goosegrass [Eleusine indica (L.) Gaertn.] and smooth crabgrass [Digitaria ischaemum (Schreb.) Schreb. ex Muhl.] are problematic weeds in bermudagrass and creeping bentgrass turf. Increased incidences of herbicide resistant weed populations and severe use restrictions on formerly available herbicides have increased need for selective, postemergence control options for these weeds in creeping bentgrass and bermudagrass turf. This weed management exigency has led turf managers to utilize less effective, more expensive, and more injurious options to manage goosegrass and smooth crabgrass. Although potentially injurious, topramezone can control these weeds, especially goosegrass, at low doses. Low-dose topramezone may also improve bermudagrass and creeping bentgrass response. An initial investigation of three 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibiting herbicides in different turf types showed that Kentucky bluegrass, perennial ryegrass, and tall fescue were highly tolerant to topramezone, while creeping bentgrass and bermudagrass could tolerate topramezone doses that may control grassy weeds. Further investigation suggested that frequent, low-dose topramezone applications or metribuzin admixtures could enhance weed control and may conserve turfgrass quality. A novel mixture of topramezone at 3.7 g ae ha-1 and metribuzin at 210 g ai ha-1 controlled goosegrass effectively and reduced bermudagrass foliar bleaching associated with topramezone 10-fold compared to higher doses of topramezone alone in 19 field and 2 greenhouse trials. In an attempt to further enhance bermudagrass tolerance to topramezone, post-treatment irrigation was applied at various timings. When bermudagrass turf was irrigated with 0.25-cm water at 15 or 30 minutes after herbicide treatment, bermudagrass injury was reduced to acceptable levels when following low-dose topramezone plus metribuzin but not when following high-dose topramezone alone. Goosegrass control was reduced significantly by post-treatment irrigation in all cases, while irrigation reduced goosegrass control by low-dose topramezone plus metribuzin to below-commercially-acceptable levels. Novel, low-dose, frequent application programs containing topramezone or siduron were developed for season-long crabgrass or goosegrass control on creeping bentgrass greens. Greens-height creeping bentgrass quality was preserved following five biweekly treatments of siduron at rates between 3,400 to 13,500 g ai ha-1 and topramezone at 3.1 g ha-1. Siduron programs controlled smooth crabgrass and suppressed goosegrass while topramezone programs controlled goosegrass and suppressed smooth crabgrass. In laboratory and controlled-environment experiments, goosegrass absorbed three times more 14C than bermudagrass within 48 hours of 14C-topramezone treatment. Bermudagrass also metabolized topramezone twice as fast as goosegrass. Metribuzin admixture reduced absorption by 25% in both species. When herbicides were placed exclusively on soil, foliage, or soil plus foliage, topramezone controlled goosegrass only when applied to foliage and phytotoxicity of both bermudagrass and goosegrass was greater from topramezone than from metribuzin. Metribuzin was shown to reduce 21-d cumulative clipping weight and tiller production of both species while topramezone caused foliar discoloration to newly emerging leaves and shoots with only marginal clipping weight reduction. These data suggest that selectivity between bermudagrass and goosegrass is largely due to differential absorption and metabolism that reduces bermudagrass exposure to topramezone. Post-treatment irrigation likely reduces topramezone rate load with a concomitant effect on plant phytotoxicity of both species. Metribuzin admixture decreases white discoloration of bermudagrass by decreased topramezone absorption rate and eliminating new foliar growth that is more susceptible to discoloration by topramezone. / Doctor of Philosophy / Goosegrass and smooth crabgrass are problematic weeds in bermudagrass and creeping bentgrass turf. Increased incidences of herbicide resistant weed populations and severe use restrictions on formerly available herbicides have increased need for selective, postemergence control options for these weeds in creeping bentgrass and bermudagrass turf. Although potentially injurious, topramezone (Pylex™) can control these weeds, especially goosegrass, at low doses. Low-dose Pylex™ may also improve bermudagrass and creeping bentgrass response. An initial investigation evaluating tembotrione (Laudis®), Pylex™, and mesotrione (Tenacity®) in different turfgrass species showed that Kentucky bluegrass, perennial ryegrass, and tall fescue were highly tolerant to Pylex™ at rates ranging from 0.75 to 2.25 fl. oz./A, while creeping bentgrass and bermudagrass were low to moderately tolerant to Pylex™. Further investigation suggested that frequent, low-dose (less than 0.25 fl. oz./A) Pylex™ applications or metribuzin (Sencor®) admixtures could enhance weed control and may conserve turfgrass quality. A novel mixture of Pylex™ at 0.15 fl. oz./A and Sencor® at 4 oz. wt./A controlled goosegrass effectively and reduced bermudagrass injury to near acceptable levels and significantly less than Pylex™ applied alone at 0.25 fl. oz/A. In an attempt to further enhance bermudagrass tolerance to Pylex™, post-treatment irrigation was applied at different timings. When bermudagrass turf was irrigated at 15 or 30 minutes after herbicide treatment, bermudagrass injury was reduced to acceptable levels when following Pylex™ at 0.25 fl. oz./A plus Sencor® at 4 oz but not when following Pylex™ applied alone at 0.5 fl. oz./A. Goosegrass control was reduced significantly by post-treatment irrigation in all cases, while irrigation reduced goosegrass control by low-dose Pylex™ plus Sencor® to below-commercially-acceptable levels. Novel, low-dose, frequent application programs containing Pylex™ or siduron (Tupersan®) were developed for season-long crabgrass or goosegrass control in creeping bentgrass greens. Greens-height creeping bentgrass quality was preserved following five biweekly treatments of Tupersan® at rates between 6 and 24 lb./A and Pylex™ at 0.125 fl. oz./A. Tupersan® programs controlled smooth crabgrass and suppressed goosegrass while Pylex™ programs controlled goosegrass and suppressed smooth crabgrass. The data from these studies indicate that utilizing low-dose Pylex™ in combination with Sencor® can impart acceptable bermudagrass safety while also controlling goosegrass effectively. For creeping bentgrass greens, the low-dose, frequent application of Tupersan® is the safest legal option for golf course superintendents to control smooth crabgrass effectively, while having some ability to suppress goosegrass.

PhD (Food Science) / Department of Food Science and Technology / Finger millet (FM) [Eleusine coracana] is an underutilised cereal grain used as a food source in South Africa. Increased research interest in FM has span over the years owing to its unique nutritional and bioactive composition. Following the recent interest in natural curative substances over their synthetic counterparts in the treatment of food dependent diseases, FM has shown potential nutraceutical effects. Some important health effects like antidiabetic, antioxidative, anti-inflammatory and antimicrobial properties have been reported in recent trials with FM. In view of the increasing utilisation and application of FM in the region of Thulamela Municipality, Vhembe District of South Africa, two common indigenous FM varieties (brown and dark brown) were obtained and analysed for their physicochemical properties, levels of minerals, phytic acid, phenolic compounds and antioxidant activities. For this process, malted non-alcoholic beverages were produced and analysed for their physicochemical properties, levels of phenolic compounds, and total phenolics and antioxidant activities. FM grains were soaked, germinated and kilned at an interval of 24 h for 96 h, using sorghum as an external reference. Mineral composition of the FM and sorghum samples were analysed using an inductively coupled plasma atomic emission spectroscopy (ICP-AES) and mass spectroscopy (ICP-MS), and atomic absorption spectrometer (AAS). Identification and quantification of phenolic compounds were performed using ultra-performance liquid chromatography mass spectrometer (UPLC-MS). All experiments were performed in triplicate except for the UPLC-MS analysis of the malted non-alcoholic beverages that was done in duplicate. Data were analysed by one way analysis of variance, and the mean values were separated by Duncan’s multiple comparison test using SPSS version 24.0. Data showed that the FM varieties were rich in macro- and micro- or trace elements. The macro-elements calcium, magnesium, potassium, phosphorus and sulphur were found in high amounts ranging from 1597.37 mg/ kg – 6775.03 mg/ kg; iron, zinc, strontium and silicon were found in significant amounts in the range 21.47 mg/ kg – 55.67 iii mg/ kg, copper and boron were found in low amounts (2.2 mg/ kg – 7.7 mg/ kg), along with selenium and cobalt (0.02 mg/ kg – 0.05 mg/ kg). Heavy metals, barium and aluminium were found in the FM varieties. Varietal difference was found to play an important role in the mineral content of the grains during malting. Malting for 24 h reduced mineral content except for sodium. Beyond 48 h of malting, mineral content increased, particularly, for 96 h in FM grain malt. Significant (p < 0.05) increases in the mineral content of FM varieties were noted at 48 h and 96 h of malting. Increase occurred at 72 h of malting for potassium, iron and boron. Malting did not have any effect on the manganese content of the dark brown FM; however, it increased the manganese content at 48 h of malting for brown FM. Malting for 96 h significantly (p < 0.05) reduced sodium content. Consecutive decrease in phytic acid content of the grains was not recorded with durations in malting time. Although statistically significant differences (p < 0.05) were observed, malting did not result in too much change in the physicochemical properties of the grains. Several flavonoids, catechin, epicatechin, quercetin, taxifolin, and hesperitin were isolated, whilst protocatechuic acid was the only phenolic acid detected in the unmalted and malted FM. Increases in catechin, epicatechin and protocatechuic acid were observed for 72 and 96 h malt of brown FM with similar observations recorded for sorghum. Complete loss of taxifolin, catechin, and hesperitin were noted with malting time. FM grains exhibited 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2՛-azinobis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging action and iron reducing activities. Increased iron reducing activity alongside ABTS radical scavenging activity was recorded with malting time. A fermentation-time dependent decrease in the pH of the non-alcoholic beverages, with a corresponding increase in sugar content were recorded. A similar decrease was also recorded for the viscosities of the beverages. The FM malt beverages were found to contain a higher amount of citric acid compared to the sorghum malt beverage. A decrease in the citric acid content with fermentation time was noted in the grain malt beverages fermented with Lactobacillus fermentum, particularly for the iv FM beverage. The phenolic compounds detected in the FM malt beverages fermented with the grain microbial flora and Lactobacillus fermentum were protocatechuic acid, catechin and epicatechin. Taxifolin and kaempferol along with the earlier mentioned compounds were detected in the sorghum malt beverage. Catechin was found in higher amount compared to other phenolic compounds in the FM and sorghum malt beverages. FM malt beverages were found to contain a higher amount of total phenolics compared to the beverage prepared from sorghum malt. Fermentation with the grains’ microbial flora and L. fermentum resulted in reduced total phenolics of FM and sorghum malt beverages, particularly after 24 h of fermentation. A fermentation-time dependent decrease in total phenolics of FM beverages fermented with L. fermentum was noted. Fermentation within 24 - 48 h using the grain microbial flora showed higher total individual phenolic compounds for the dark brown FM and sorghum, compared to other fermentation periods. Fermentation of the beverages for 24 h retained a higher amount of the total phenolics compared to other fermentation periods, especially for the L. fermentum beverages. Reduced total phenolic content and antioxidant activity of the beverages were noted at 24 h of fermentation for the two microbial sources. Significant (p < 0.05) increases in total phenolics were observed within 72 – 96 h of fermentation of the brown FM malt beverage with the grains’ microbial flora. Fermentation for 72 h and 96 h with L. fermentum increased the total phenolic content of the brown FM. Increase in total flavonoid content (TFC) of brown FM malt beverage was noted at 72 h fermentation for both microbial sources. Unlike with L. fermentum, no significant (p > 0.05) change in TFC was observed for the dark brown FM beverage after 24 h fermentation with the grains’ microbial flora. Beverages exhibited DPPH, ABTS radical scavenging action and iron reducing activities, which were significantly (p < 0.05) reduced at 96 h fermentation for both microbial sources. The 24 h fermented beverage retained a higher amount of total phenolic and flavonoid contents, and had higher antioxidant activity compared to other fermentation periods for both microbial sources. The study shows that FM is a rich source of essential minerals and v phenolic compounds, and demonstrates that 72 to 96 h of malting has a positive effect on minerals and certain phenolic compounds over the 48 h malting period widely used for preparation of FM malt. The presence of hesperitin in FM grain was established. A new method was developed for the production of FM non-alcoholic beverage with measurable amounts of health-promoting compounds. An ideal fermentation period (24 h) for FM malt non-alcoholic beverage production with enhanced health-promoting compounds, using Lactobacillus fermentum was demonstrated. Fermentation limit (96 h) for production of FM malt beverage using either the grain microbial flora or L. fermentum was confirmed. These findings provide a rationale for increased utilisation of FM as a functional food grain, and its use as malt in production of non-alcoholic beverage for health promotion and wellness. / NRF

Submitted by Johnny Rodrigues (johnnyrodrigues@ufcg.edu.br) on 2018-05-15T17:40:46Z No. of bitstreams: 1 JOÃO JONES DA SILVA - DISSERTAÇÃO PPGSA PROFISSIONAL 2015..pdf: 1781652 bytes, checksum: 5ecbeafd5b48184bce3785273190c6dd (MD5) / Made available in DSpace on 2018-05-15T17:40:46Z (GMT). No. of bitstreams: 1 JOÃO JONES DA SILVA - DISSERTAÇÃO PPGSA PROFISSIONAL 2015..pdf: 1781652 bytes, checksum: 5ecbeafd5b48184bce3785273190c6dd (MD5) Previous issue date: 2015-09-29 / A Região Nordeste do Brasil é caracterizada por clima semiárido, sendo os volumes precipitados normalmente inferiores à evaporação e, tornando o uso da irrigação imprescindível para uma produção agrícola sustentável, entretanto, o manejo inadequado da irrigação atrelado às condições locais, tem favorecido os processos de salinização e sodificação de solos, promovendo a degradação e o abandono de extensas áreas anteriormente produtivas. Entre as áreas afetadas por sais nessa região, destaca-se o Perímetro Irrigado de São Gonçalo - PB em situação de degradação de solos com problemas de sais, isso ensejou a propositura de estudar métodos e técnicas visando o melhoramento das características físicas e químicas do solo. O experimento foi instalado em área previamente identificada como possuidora de solo salino-sódico no Perímetro Irrigado de São Gonçalo, e para a recuperação fez-se da aplicação dos métodos e da técnica da fitorremediação com Erva-Sal (Atriplex numulária L.), Capim-pé-de-galinha (Eleusine indica L.), Salsa - Brava (Ipomoea asarifolia L.) e Gesso agrícola. A pesquisa constou de oito tratamentos, com quatro repetições, os tratamentos foram submetidos a um delineamento em blocos casualizados (DBC), com os seguintes tratamentos: i) cultivo de atriplex; ii) cultivo de capim-pé-galinha, iii) cultivo de salsa, iv) solo com gesso; v)aplicação de gesso agrícola associado ao cultivo de atriplex; vi)aplicação de gesso agrícola associado ao cultivo de capim pé-de-galinha; vii)aplicação de gesso agrícola associado ao cultivo de salsa e viii) solo sem manejo (testemunha), numa área total de 62 m² conduzido em campo, em parcelas de 1,40 x 1,40 m. Com esse estudo pretende-se recuperar solo afetados por sais, utilizando um método alternativo, mais viável economicamente e sustentável através de plantas capazes de extrair os sais em excesso do solo. / Brazil's Northeast region is characterized by semi-arid climate, and the precipitated volumes usually lower than the evaporation and, making use of vital irrigation for sustainable agricultural production, however, inadequate irrigation management linked to local conditions, has favored processes salinization and sodification soils, promoting the degradation and abandonment of extensive formerly productive areas. Among the areas affected by salts in this region, we highlight the Irrigated Perimeter of São Gonçalo - PB in soil degradation situation with salt problems and this gave rise to the filing of study methods and techniques aimed at improving the physical and chemical characteristics ground. The experiment was installed in previously identified area as saline-sodic soil possessed in the Irrigated Perimeter of São Gonçalo, and the recovery was made of the application of methods and phytoremediation technique with herb-salt (Atriplex moneywort L.), grass crow's foot bath (Eleusine indica L.), Salsa - Brava (Ipomoea asarifolia L.) and Agricultural Gypsum. The survey consisted of eight treatments, with four replications, the treatments were subjected to a randomized block design (RBD), with the following treatments: i) atriplex cultivation; ii) grass-foot-chicken farming, iii) salsa culture, iv) soil with gypsum; v) application of gypsum associated with atriplex cultivation; vi) application of gypsum associated with grass growing chicken's foot; vii) application of gypsum associated with the parsley cultivation and viii) soil without management (control), a total area of 62 m² conducted under field conditions in portions of 1.40 x 1.40 m. With this study we intend to recover salt affected soil, using an alternative method more economically viable and sustainable through plants capable of extracting soil excess salts.