Type IV pili (T4P) are protein filaments found on the surface of a variety of bacterial species and mediate biofilm formation, adhesion, and flagellum-independent twitching motility. The biogenesis of T4P is dependent on a cell envelope-spanning, multiprotein complex that localizes to the poles in rod-shaped cells. How these proteins localize and cross the peptidoglycan (PG) layer in the absence of dedicated PG-hydrolyzing enzymes is unknown. In P. aeruginosa, PilMNOP interact to form the alignment subcomplex, connected via PilP to PilQ, which forms the outer membrane secretin. We hypothesized that polar localization and integration of the T4P machinery was driven by ordered recruitment to future sites of cell division, placing assembly system components at division septa in the correct position before daughter-cell separation. To determine which T4P components are essential for localization of the complex, we fused the T4P inner membrane assembly protein PilO to the fluorescent protein mCherry to monitor its localization. mCherry-PilO localized to the cell poles and midcell in wild type bacteria. However, it was delocalized in a strain lacking PilQ. A PilQ-mCherry fusion localized to the cell poles, likely through its putative septal PG binding AmiN domains, suggesting that PilQ binds PG and thus localizes its partners to future sites of cell division. In the absence of the associated pilotin protein (PilF), which is required for PilQ multimerization in the OM, T4P components were polarly localized, implying that localization is not dependent on secretin formation. The results of this research support a pre-installation mechanism for integration of protein complexes in the gram negative cell envelope without PG hydrolysis, which may be applicable to other systems. / Thesis / Master of Science (MSc)

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The thesis investigates pipeline plugging mechanisms that have occurred during interim stabilization transfers at Hanford. A laboratory-scale saltwell pumping test loop was designed to evaluate a surrogate of Hanford Tank 241-SX-104 supernate. The effect of surrogate flow rate, cooling water flow rate and phosphate concentrations on plugging mechanisms was investigated. Critical parameters like particle and agglomerate size, velocity and bed growth rate were determined. Theoretical models were used to compare the experimental pressure rise and temperature drop of the surrogate in the channel. An operating region in which a plug would not form was developed, based on the experimental results. Experiments are also reported on plug remediation. Unplugging experiments at varying pump pressure heads and residence time of plug in the line were performed.

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No / A printed crescent-shaped monopole MIMO antenna is presented for handheld wireless communication devices. The mutual coupling between the two antenna elements can be minimised by implementing a I-shaped common radiator. Both the simulated and measured results agree that the antenna covers the operating frequency band from 1.6 to 2.8 GHz with the return loss and isolation better than 10 dB and 14 dB respectively. To further verifying the MIMO characteristic including far-field, gain, radiation efficiency, channel capacity loss and envelope correlation, the results confirm that the antenna can operate effectively in a rich multipath environment.

yes / The envelope correlation for a two-element antenna array may be calculated using the antenna radiation fields, or more simply from the scattering parameters of the system. The use of scattering parameters provides a major simplification over the direct use of field data. In this paper we propose a modification of the scattering parameter method which also includes the antenna losses. This approach has the advantage of simplifying the antenna design process, especially when low envelope correlations are needed. It also offers a better prediction of the spatial envelope correlation, and a good framework for understanding the effects of the mutual coupling. The accuracy of this proposed method is illustrated by two examples.