京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第18330号 / 農博第2055号 / 新制||農||1022(附属図書館) / 学位論文||H26||N4837(農学部図書室) / 31188 / 京都大学大学院農学研究科応用生物科学専攻 / (主査)教授 荒井 修亮, 教授 山下 洋, 准教授 笠井 亮秀 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

Chromium is used in many processing applications, which has led to the formation of chromium(VI) waste. Cr(VI) is an unstable, mobile carcinogen, which is interchangeable with Cr(III) under certain environmental conditions. Management of this waste, however, is often not considered. Mine under investigation is an example of such historic mismanagement. During the second World War, Cr(VI) waste was transferred to the study site from areas where leather products were made for the war effort. This waste was not managed appropriately in the past and with time Cr(VI) leached into the groundwater and possibly surface water resources. As these water resources are used for domestic and agricultural water supply, this may have serious effects on the human and environmental health in the area. Some of the major Cr(VI) effects on human and animal health include malignant tumours, skin irritation, respiratory and reproductive system damage. Lung cancer is of concern when Cr(VI) is inhaled and stomach tumours occur when this chemical pollutant is ingested. The effects of Cr(VI) on plants include the disruption of shoot and root elongation, and if it accumulates enough within the plant can cause ingested health problems for humans and animals. Water quality guidelines state that the Target Water Quality Range for Cr(VI) in drinking water should not exceed 0.05 mg/L. Prolonged exposure of values higher than this target value has adverse health effects and may result in cancer. The study site has a scarcity in water sources and therefore requires good quality water resources. This study aims to identify and use biomarkers to assist in the hydrological characterization of the mine. Available mitigation options can be implemented once it is known how the water in the area moves and distributes Cr(VI) pollution. A biomarker is an indicator of a biological state, which in turn can be used to assist in characterizing the chemical conditions of the sub-surface. Bacteria can aid as environmental biomarkers as they are sensitive and specific to the environmental conditions in which they flourish. In this way they give a good indication of the environmental condition and any possible pollution. Due to the fact that ground- and surface water are integrated resources, it is likely that if one is impacted by pollution, it will indirectly impact the other one. Therefore, the biomarkers identified can be used to characterize water pollutants that are present in ground- and some surface water resources. A description of the study site is provided, wherein the climate, elevation, geology, land use, geohydrology, hydrochemistry and surface water are documented. These factors help to identify and clarify the sources and pathways that water and the pollution would follow. Ten water samples, from surface and groundwater, were obtained in two separate sampling opportunities. The first analysis of the water samples included the determination of the chemical constituents. Two of these constituents analyzed were the total Cr and individual Cr(VI) levels. Six water samples had excessively high Cr values (exceeding the Water Quality Target Range of drinking water and water agricultural use). These values ranged from 0.1 – 3.9 mg/L. The 6 samples with excessive Cr(VI) values were used for the microbial analyses. The microbial analyses consisted of DAPI (4’,6-diamidino-2-phenylindole ) staining, for cell enumeration, and molecular analyses. The molecular analyses included polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and sequencing applications concluded in the laboratory. Fifteen bands, representing different organisms, were removed from the DGGE polyacrylamide gel and processed for sequencing. The organisms that were present in the sample were uncultured Cyanobacterium, Sediminibacterium salmoneum, uncultured Bacteroidetes bacterium, uncultured betaproteobacterium, uncultured actinobacterium, uncultured Rhodocyclaceae, uncultured Chloroflexi bacterium and uncultured delta-proteobacterium. According to literature most of these organisms may adapt the ability to either reduce Cr(VI) or resist any effect of Cr(VI) in the environment. Two of the bands were highly unidentified organisms, which means that these organisms have not yet been cultured or identified in any sense. The reason for this is that most microorganisms have not yet been documented. This also makes it difficult to identify the exact bacterial strain present within the samples. The deoxyribonucleic acid (DNA) sequences of the different organisms were very different from each other, when compared by a dendrogram. This means that there was a diverse community present within the samples. Electrical conductivity profiles were conducted in the monitoring boreholes to identify possible fracture positions. The total chromium and chromium(VI) levels were documented and compared. Other chemical factors were analysed and those of high value, such as chloride, nitrate and chromium measurements, were used for statistical analyses and comparison with the biomarkers present in the sample. A positive correlation was found between the sample sites and the organisms present within each. It was noted that different communities have different metabolic activities related to susceptibility and will therefore differ under specific environmental conditions. The microorganisms that were present in the 6 water samples all have the ability to either resist or reduce Cr(VI). This means that in Cr(VI) polluted areas they are more likely to flourish than organisms that do not possess this ability. Such susceptible, non-resistant organisms would otherwise occur naturally in a non-polluted environment. From the obtained results it was noted that microorganisms could aid as biomarkers when determining the environmental condition (with respect to Cr(VI) pollution). The bacteria analysed in the samples all indicate a level of chromium pollution, and aided in the determination of pollution sources. These biomarkers can therefore be used to determine the location of other chromium deposits not yet located. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2013

Chromium is used in many processing applications, which has led to the formation of chromium(VI) waste. Cr(VI) is an unstable, mobile carcinogen, which is interchangeable with Cr(III) under certain environmental conditions. Management of this waste, however, is often not considered. Mine under investigation is an example of such historic mismanagement. During the second World War, Cr(VI) waste was transferred to the study site from areas where leather products were made for the war effort. This waste was not managed appropriately in the past and with time Cr(VI) leached into the groundwater and possibly surface water resources. As these water resources are used for domestic and agricultural water supply, this may have serious effects on the human and environmental health in the area. Some of the major Cr(VI) effects on human and animal health include malignant tumours, skin irritation, respiratory and reproductive system damage. Lung cancer is of concern when Cr(VI) is inhaled and stomach tumours occur when this chemical pollutant is ingested. The effects of Cr(VI) on plants include the disruption of shoot and root elongation, and if it accumulates enough within the plant can cause ingested health problems for humans and animals. Water quality guidelines state that the Target Water Quality Range for Cr(VI) in drinking water should not exceed 0.05 mg/L. Prolonged exposure of values higher than this target value has adverse health effects and may result in cancer. The study site has a scarcity in water sources and therefore requires good quality water resources. This study aims to identify and use biomarkers to assist in the hydrological characterization of the mine. Available mitigation options can be implemented once it is known how the water in the area moves and distributes Cr(VI) pollution. A biomarker is an indicator of a biological state, which in turn can be used to assist in characterizing the chemical conditions of the sub-surface. Bacteria can aid as environmental biomarkers as they are sensitive and specific to the environmental conditions in which they flourish. In this way they give a good indication of the environmental condition and any possible pollution. Due to the fact that ground- and surface water are integrated resources, it is likely that if one is impacted by pollution, it will indirectly impact the other one. Therefore, the biomarkers identified can be used to characterize water pollutants that are present in ground- and some surface water resources. A description of the study site is provided, wherein the climate, elevation, geology, land use, geohydrology, hydrochemistry and surface water are documented. These factors help to identify and clarify the sources and pathways that water and the pollution would follow. Ten water samples, from surface and groundwater, were obtained in two separate sampling opportunities. The first analysis of the water samples included the determination of the chemical constituents. Two of these constituents analyzed were the total Cr and individual Cr(VI) levels. Six water samples had excessively high Cr values (exceeding the Water Quality Target Range of drinking water and water agricultural use). These values ranged from 0.1 – 3.9 mg/L. The 6 samples with excessive Cr(VI) values were used for the microbial analyses. The microbial analyses consisted of DAPI (4’,6-diamidino-2-phenylindole ) staining, for cell enumeration, and molecular analyses. The molecular analyses included polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and sequencing applications concluded in the laboratory. Fifteen bands, representing different organisms, were removed from the DGGE polyacrylamide gel and processed for sequencing. The organisms that were present in the sample were uncultured Cyanobacterium, Sediminibacterium salmoneum, uncultured Bacteroidetes bacterium, uncultured betaproteobacterium, uncultured actinobacterium, uncultured Rhodocyclaceae, uncultured Chloroflexi bacterium and uncultured delta-proteobacterium. According to literature most of these organisms may adapt the ability to either reduce Cr(VI) or resist any effect of Cr(VI) in the environment. Two of the bands were highly unidentified organisms, which means that these organisms have not yet been cultured or identified in any sense. The reason for this is that most microorganisms have not yet been documented. This also makes it difficult to identify the exact bacterial strain present within the samples. The deoxyribonucleic acid (DNA) sequences of the different organisms were very different from each other, when compared by a dendrogram. This means that there was a diverse community present within the samples. Electrical conductivity profiles were conducted in the monitoring boreholes to identify possible fracture positions. The total chromium and chromium(VI) levels were documented and compared. Other chemical factors were analysed and those of high value, such as chloride, nitrate and chromium measurements, were used for statistical analyses and comparison with the biomarkers present in the sample. A positive correlation was found between the sample sites and the organisms present within each. It was noted that different communities have different metabolic activities related to susceptibility and will therefore differ under specific environmental conditions. The microorganisms that were present in the 6 water samples all have the ability to either resist or reduce Cr(VI). This means that in Cr(VI) polluted areas they are more likely to flourish than organisms that do not possess this ability. Such susceptible, non-resistant organisms would otherwise occur naturally in a non-polluted environment. From the obtained results it was noted that microorganisms could aid as biomarkers when determining the environmental condition (with respect to Cr(VI) pollution). The bacteria analysed in the samples all indicate a level of chromium pollution, and aided in the determination of pollution sources. These biomarkers can therefore be used to determine the location of other chromium deposits not yet located. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2013

Erosion is an issue, which causes huge damages not only on agricultural land. This diploma thesis describes practical evaluation of erosion control measures (usage of leaving crop residue, circuit strips, contour tillage), which are include in Good Agriculture and Environmental Condition standart, which was issued by European union. Efficiency of usage of narrow-row and wide-row crops is also modeled. Each measures were evaluated in two different modeling programmes HydroCad for calibration and ERCN for results comparison. Seven different scripts are used in the thesis. Subcatchment area P6 in catchment area of Kopaninský creek is used as model area. Literature review describes the precipitation and runoff process, issue of erosion, and agriculture land protection against water erosion. Is also describes GAEC standards (today DZES).

Självreglerat lärande (SRL) har visat sig vara en användbar strategi för både idrottslig inlärning och utveckling. Vilka miljömässiga förutsättningar idrottaren ges kan både främja och hämma denna utveckling, vilket få studier undersökt i en idrottslig kontext. Studiens syfte var därav att undersöka grad av SRL inom individuell idrott utifrån self-efficacy, könsidentitet och miljömässiga förutsättningar. Miljömässiga förutsättningar innefattade ledarskapsbeteenden som främjar både motivation, enligt Self-determination theory (SDT), och självreglerat lärande. Urvalet bestod av individuella idrottare, 16–60 år, som har en tränare (N = 251). Dessa tävlade inom 28 olika individuella idrotter och identifierade sig som kvinnor (n = 144), män (n = 106) och annat (n = 1). Frågeställningarna besvarades med hjälp av självskattningsformulären Self-Regulated Learning in Sport Practice (SRL-SP), Self-Regulated Environment (SRE) och Interpersonal Supportiveness Scale - Coach (ISS-C). Resultat från multipla och hierarkiska regressionsanalyser indikerade att self-efficacy hade ett positivt samband med utfallsmåtten planering, övervakning och reflektion, men inte ansträngning. Könsidentitet verkade inte moderera denna effekt. Vad gäller miljömässiga förutsättningar bidrog främst tränares förmåga att skapa möjligheter för SRL till idrottares självreglering. Samtidigt visades att idrottarnas grad av SRL påverkades av tränarens närvaro; ju mer närvarande tränare desto lägre grad av självregleringsstrategier uppvisar idrottarna. Slutsatsen blir därmed att idrottare verkar behöva ha en tilltro till sin förmåga samt befinna sig i en miljö med möjligheter för SRL, för att engagera sig i sin idrottsliga utveckling på ett fördelaktigt sätt. Detta samband verkar även påverkas av tränarens fysiska närvaro. Framtida studier kan med fördel vidare undersöka påverkan av de miljömässiga förutsättningarna på grad av SRL, samt om det skiljer sig åt beroende på idrott. / Self-Regulated Learning (SRL) has proven to be a useful strategy for athletes' learning and development. What conditions are given to athletes from their surrounding environment can both promote and inhibit these processes of learning and development. However, few studies have examined this relationship in a sports context. Thus, the present study aimed to investigate Self-Regulated Learning in individual sports based on self-efficacy, gender and environmental conditions. The environmental conditions were defined as leadership behaviors that promote motivation, according to Self-Determination Theory (SDT), and Self-Regulated Learning. The sample consisted of individual athletes, ranging from 16-60 years, with a coach (N = 251). The athletes competed in 28 different individual sports and identified themselves as women (n = 144), men (n = 106) and other (n = 1). The participants answered the self-report questionnaires Self-Regulated Learning in Sport Practice (SRL-SP), Self-Regulated Environment (SRE) and Interpersonal Supportiveness Scale - Coach (ISS-C). Using multiple and hierarchical regression analyses, this study provided support that self-efficacy positively influenced the outcome measures planning, monitoring, and reflection, but not effort. Gender did not appear to moderate this relationship. The environmental conditions associated with SRL was mainly the coaches' ability to create opportunities for SRL. Additionally, athletes' SRL were negatively influenced by how often the coach was present. The conclusion is that athletes, to beneficially engage in their own development, need to have a belief in their own ability and also be in an environment that enhances opportunities for SRL. However, this relationship is influenced by the coach's presence at practice. Future studies can further examine the relationship between the environmental conditions and SRL, and if the results may differ depending on sport.