<p>U ovoj doktorskoj disertaciji prikazani su rezultati istraživanja 15&nbsp;vrsta&nbsp; biljaka&nbsp; koje&nbsp; pripadaju&nbsp; rodovima <em>Rumex,&nbsp; Polygonum,&nbsp;Bistorta,&nbsp; Persicaria i&nbsp; Fagopyrum,</em>&nbsp; podfamilije&nbsp; Polygonoideae,&nbsp;familije&nbsp; Polygonaceae,&nbsp; sakupljenih&nbsp; na&nbsp; teritoriji&nbsp; centralnog&nbsp; i&nbsp;zapadnog &nbsp;Balkana u periodu od 2009-2011. godine. Sprovedena&nbsp;istraživanja&nbsp; su&nbsp; se&nbsp; odvijala&nbsp; u&nbsp; dva&nbsp; pravca:&nbsp; fitohemijska&nbsp; i&nbsp;biohemijsko-biolo&scaron;ka&nbsp; ispitivanja.&nbsp; Predmet&nbsp; analiza&nbsp; bili&nbsp; su&nbsp;ekstrakti&nbsp; herbi&nbsp; i&nbsp; rizoma&nbsp; ispitivanih&nbsp; biljaka.&nbsp; Fitohemijska&nbsp;ispitivanja&nbsp; obuhvatila&nbsp; su,&nbsp; pored&nbsp; spektrofotometrijskog&nbsp;određivanja&nbsp; ukupnih&nbsp; fenola,&nbsp; ukupnih&nbsp; flavonoida&nbsp; i&nbsp; ukupnih&nbsp;antrahinonskih jedinjenja, i određivanje sadržaja 51 komponente&nbsp;iz standardne sme&scaron;e različitih klasa fenolnih jedinjenja LC-MSMS&nbsp; metodom,&nbsp; odnosno &nbsp;hromatografsko&nbsp; profilisanje&nbsp; ekstrakata&nbsp;LC-DAD-MS&nbsp; metodom.&nbsp; Odabirom&nbsp; nekoliko&nbsp; različitih&nbsp; model&nbsp;sistema&nbsp; za&nbsp; merenje&nbsp; antioksidantne&nbsp; aktivnosti&nbsp; (neutralizacija&nbsp;DPPH&nbsp; radikala,&nbsp; redoks&nbsp; kapacitet&nbsp; -&nbsp; FRAP&nbsp; test,&nbsp; skevindžer&nbsp;aktivnost&nbsp; prema&nbsp; superoksidanjon&nbsp; radikalu,&nbsp; NO&nbsp; radikalu&nbsp; i&nbsp; OH&nbsp;radikalu,&nbsp; kao&nbsp; i&nbsp; inhibicija&nbsp; lipidne&nbsp; peroksidacije)&nbsp; procenjen&nbsp; je&nbsp;antioksidantni&nbsp; potencijal&nbsp; ekstrakata,&nbsp; dok&nbsp; je&nbsp; za&nbsp; procenu&nbsp; njihove&nbsp;antiinflamatorne&nbsp; aktivnosti&nbsp; kori&scaron;ćen&nbsp; potencijal&nbsp; inhibicije&nbsp;biosinteze medijatora inflamacije u humanim trombocitima (kao&nbsp;model&nbsp; sistemu).&nbsp; Mikrobiolo&scaron;ka&nbsp; ispitivanja&nbsp; su&nbsp; obuhvatila&nbsp;određivanje&nbsp; potencijala&nbsp; ovih&nbsp; vrsta&nbsp; u&nbsp; inhibiciji&nbsp; rasta&nbsp; serije&nbsp; gram&nbsp;pozitivnih i gram negativnih sojeva batkerija. Konačno, urađena&nbsp;je&nbsp; analiza&nbsp; korelacije&nbsp; hemijskog&nbsp; sastava,&nbsp; biolo&scaron;ke&nbsp; aktivnosti&nbsp; i&nbsp;pripadnosti taksonomskim grupama.</p> / <p>Phytochemical&nbsp; and&nbsp; biochemical&nbsp; analysis&nbsp; of&nbsp; herbal&nbsp; and&nbsp; root&nbsp;ethanol&nbsp; extracts&nbsp; of&nbsp; 15&nbsp; species&nbsp; belonging&nbsp; to&nbsp; different&nbsp; genera&nbsp;(<em>Rumex,&nbsp; Polygonum,&nbsp; Bistorta,&nbsp; Persicaria and&nbsp; Fagopyrum</em>)&nbsp; of&nbsp;subfamily&nbsp; Polygonoideae,&nbsp; was&nbsp; examined.&nbsp; Phytochemical&nbsp;characterization&nbsp; included&nbsp; spectrophotometric&nbsp; determination&nbsp; of&nbsp;total&nbsp; phenolic,&nbsp; total&nbsp; flavonoids&nbsp; and&nbsp; total&nbsp; anthraquinone&nbsp; contents,&nbsp;quantification&nbsp; of&nbsp; 51&nbsp; secondary&nbsp; metabolites&nbsp; by&nbsp; LC/MS/MS&nbsp;analysis&nbsp; and&nbsp; chromatographic&nbsp; fingerprinting by&nbsp; LC/DAD/MS&nbsp;technique,&nbsp; of&nbsp; prepared&nbsp; extracts.&nbsp; The&nbsp; antioxidant&nbsp; activity&nbsp; was&nbsp;evaluated&nbsp; by&nbsp; measuring&nbsp; ferric&nbsp; reducing&nbsp; ability&nbsp; (FRAP)&nbsp; of&nbsp; the&nbsp;extracts and their radical scavenging capacity towards DPPH, OH,&nbsp;NO and O₂^{&ndash;&nbsp;}radicals, and inhibition of lipid peroxidation). Antiinflammatory activity was evaluated by LC/MS/MS monitoring of&nbsp;selected&nbsp; metabolites&nbsp; (12-(S)-HHT,&nbsp; 12(S)-HETE,&nbsp; PGE_2&nbsp;,&nbsp; PGF_2&alpha;,&nbsp;and TXB₂) formed in cyclooxygenase and lipoxygenase pathways&nbsp;of arachidonic acid metabolism. Human platelets were used as a&nbsp;source&nbsp; of&nbsp; enzymes,&nbsp; while&nbsp; inflammation&nbsp; was&nbsp; induced&nbsp; by&nbsp;calcimycin. The antibacterial activity of prepared&nbsp; extracts against&nbsp;nine&nbsp; bacterial&nbsp; strains&nbsp; was&nbsp; evaluated&nbsp; by&nbsp; microtiter&nbsp; assay&nbsp; with&nbsp;resazurin as a colorimetric growth indicator.</p>

This study investigated understorey management in Canterbury, New Zealand, apple orchards for the enhancement of populations of Dolichogenidea tasmanica (Cameron) (Braconidae) for leafroller (Lepidoptera: Tortricidae) biological control. The first objective was to determine the influence of understorey plants on the abundance of D. tasmanica and leafroller parasitism, and to investigate the mechanisms behind this influence. The second was to determine the most suitable understorey plants in terms of their ability to enhance parasitoid abundance, leafroller parasitism, parasitoid longevity, parasitoid fecundity and its ability to not benefit leafroller. Results from three consecutive field trials showed that buckwheat (Fagopyrum esculentum Moench), coriander (Coriandrum sativum L.), alyssum (Lobularia maritima (L.) Desv), and, to a lesser extent, broad bean (Vicia faba L.), enhanced parasitoid abundance and leafroller parasitism. The mechanisms behind the effects of understorey plants had previously been unexplored. However, results here showed that it was the flowers or the buckwheat that 'attracted' the parasitoid to the plant and not the shelter, aphids or microclimate that the plant may also provide. Providing flowering plants in the orchard understorey also increased immigration of parasitoids and enhanced parasitoids and enhanced parasitoid longevity and fecundity in the laboratory. In contrast, the understorey plants had no influence on the female:male ratio of D. tasmanica. Although coriander enhanced leafroller parasitism three-fold in field experiments compared with controls, it failed to enhance the longevity of both sexes of D. tasmanica in the laboratory compared with water-only. Broad bean significantly enhanced parasitoid abundance three-fold and significantly increased parasitism from 0% to 75% compared with the controls on one leafroller release date. However, laboratory trials showed that of male D. tasmancia but it did not enhance female longevity. Also, female D. tasmanica foraging on broad bean produced a total of only three parasitoid cocoons, but this result was based on an overall 6.5% survival of larvae to pupae or to parasitoid cocoon. Furthermore, results suggested that extrafloral nectar secretion decreased as the plants matured. Phacelia (Phacelia tanacetifolia Benth.) did not significantly enhance parasitism rate in the field compared with controls, and numbers of D. tasmanica captured by suction sampling were significantly lower in phacelia treatments compared with alyssum, buckwheat and control plots. Also, laboratory experiments showed that survival of D. tasmanica on phacelia flowers was equivalent to that on water-only and significantly lower than on buckwheat. These results suggest that phacelia does not provide nectar to D. tasmanica, only pollen, and therefore is not a suitable understorey plant for D. tasmanica enhancement in orchards. Buckwheat and alyssum showed the most potential as understorey plants for the enhancement of natural enemies. Buckwheat not only increased numbers of D. tasmanica seven-fold, but also increased numbers of beneficial lacewings (Micromus tasmaniae (Walker)) and hover flies (Syrphidae) captured on yellow sticky traps compared with the controls. It significantly increased leafroller parasitism by D. tasmanica from 0% to 86% compared with the controls (on one date only), and in the laboratory enhanced D. tasmanica longevity and increased fecundity compared with water-only. Similarly, alyssum significantly increased parasitism rate compared with controls, and two-fold more D. tasmanica were suction sampled in these plots compared with controls. It also enhanced longevity of both sexes of D. tasmanica compared with water, and showed the most favourable characteristics in terms of being of no benefit to leafrollers. This is because it was not preferred over apple by leafroller larvae and when they were forced to feed on it, it caused high mortality (94.3%) and low pupal weight (15 mg). Furthermore, alyssum did not enhance the number of fertile eggs produced by adult leafrollers compared with water only. However, further research is required to address the overall effect of buckwheat and alyssum on crop production and orchard management, including effects on fruit yield and quality, frost risk, disease incidence, soil quality, weeds and other pests. Also, research into the ability of these plants to survive in the orchard with little maintenance, and into the optimal sowing rates, would be useful. Sampling natural populations of leafroller within each treatment showed that damage from leafrollers and the number of leafroller larvae were respectively 20.3% and 29.3% lower in the flowering treatments compared with the controls. Furthermore, field trials showed up to a six-fold increase in leafroller pupae in controls compared with buckwheat and alyssum. This suggests that increasing leafroller parasitism rate from understorey management in orchards will translate into lower pest populations, although neither larval numbers/damage nor pupal numbers differed significantly between treatments. Trapping D. tasmanica at a gradient of distances showed that this parasitoid travels into rows adjacent to buckwheat plots, indicating that growers may be able to sow flowering plants in every second or third row of the orchard, and still enhance leafroller biocontrol while minimising the adverse effects of a cover crop. Sowing buckwheat and alyssum in orchard understoreys may enhance biological control of apple pests in organic apple production and reduce the number of insect growth regulators applied in IFP programmes. However, the challenge still remains to investigate whether conservation biological control can reduce leafroller populations below economic thresholds.

This study investigated understorey management in Canterbury, New Zealand, apple orchards for the enhancement of populations of Dolichogenidea tasmanica (Cameron) (Braconidae) for leafroller (Lepidoptera: Tortricidae) biological control. The first objective was to determine the influence of understorey plants on the abundance of D. tasmanica and leafroller parasitism, and to investigate the mechanisms behind this influence. The second was to determine the most suitable understorey plants in terms of their ability to enhance parasitoid abundance, leafroller parasitism, parasitoid longevity, parasitoid fecundity and its ability to not benefit leafroller. Results from three consecutive field trials showed that buckwheat (Fagopyrum esculentum Moench), coriander (Coriandrum sativum L.), alyssum (Lobularia maritima (L.) Desv), and, to a lesser extent, broad bean (Vicia faba L.), enhanced parasitoid abundance and leafroller parasitism. The mechanisms behind the effects of understorey plants had previously been unexplored. However, results here showed that it was the flowers or the buckwheat that 'attracted' the parasitoid to the plant and not the shelter, aphids or microclimate that the plant may also provide. Providing flowering plants in the orchard understorey also increased immigration of parasitoids and enhanced parasitoids and enhanced parasitoid longevity and fecundity in the laboratory. In contrast, the understorey plants had no influence on the female:male ratio of D. tasmanica. Although coriander enhanced leafroller parasitism three-fold in field experiments compared with controls, it failed to enhance the longevity of both sexes of D. tasmanica in the laboratory compared with water-only. Broad bean significantly enhanced parasitoid abundance three-fold and significantly increased parasitism from 0% to 75% compared with the controls on one leafroller release date. However, laboratory trials showed that of male D. tasmancia but it did not enhance female longevity. Also, female D. tasmanica foraging on broad bean produced a total of only three parasitoid cocoons, but this result was based on an overall 6.5% survival of larvae to pupae or to parasitoid cocoon. Furthermore, results suggested that extrafloral nectar secretion decreased as the plants matured. Phacelia (Phacelia tanacetifolia Benth.) did not significantly enhance parasitism rate in the field compared with controls, and numbers of D. tasmanica captured by suction sampling were significantly lower in phacelia treatments compared with alyssum, buckwheat and control plots. Also, laboratory experiments showed that survival of D. tasmanica on phacelia flowers was equivalent to that on water-only and significantly lower than on buckwheat. These results suggest that phacelia does not provide nectar to D. tasmanica, only pollen, and therefore is not a suitable understorey plant for D. tasmanica enhancement in orchards. Buckwheat and alyssum showed the most potential as understorey plants for the enhancement of natural enemies. Buckwheat not only increased numbers of D. tasmanica seven-fold, but also increased numbers of beneficial lacewings (Micromus tasmaniae (Walker)) and hover flies (Syrphidae) captured on yellow sticky traps compared with the controls. It significantly increased leafroller parasitism by D. tasmanica from 0% to 86% compared with the controls (on one date only), and in the laboratory enhanced D. tasmanica longevity and increased fecundity compared with water-only. Similarly, alyssum significantly increased parasitism rate compared with controls, and two-fold more D. tasmanica were suction sampled in these plots compared with controls. It also enhanced longevity of both sexes of D. tasmanica compared with water, and showed the most favourable characteristics in terms of being of no benefit to leafrollers. This is because it was not preferred over apple by leafroller larvae and when they were forced to feed on it, it caused high mortality (94.3%) and low pupal weight (15 mg). Furthermore, alyssum did not enhance the number of fertile eggs produced by adult leafrollers compared with water only. However, further research is required to address the overall effect of buckwheat and alyssum on crop production and orchard management, including effects on fruit yield and quality, frost risk, disease incidence, soil quality, weeds and other pests. Also, research into the ability of these plants to survive in the orchard with little maintenance, and into the optimal sowing rates, would be useful. Sampling natural populations of leafroller within each treatment showed that damage from leafrollers and the number of leafroller larvae were respectively 20.3% and 29.3% lower in the flowering treatments compared with the controls. Furthermore, field trials showed up to a six-fold increase in leafroller pupae in controls compared with buckwheat and alyssum. This suggests that increasing leafroller parasitism rate from understorey management in orchards will translate into lower pest populations, although neither larval numbers/damage nor pupal numbers differed significantly between treatments. Trapping D. tasmanica at a gradient of distances showed that this parasitoid travels into rows adjacent to buckwheat plots, indicating that growers may be able to sow flowering plants in every second or third row of the orchard, and still enhance leafroller biocontrol while minimising the adverse effects of a cover crop. Sowing buckwheat and alyssum in orchard understoreys may enhance biological control of apple pests in organic apple production and reduce the number of insect growth regulators applied in IFP programmes. However, the challenge still remains to investigate whether conservation biological control can reduce leafroller populations below economic thresholds.

Arthropod species that have the potential to damage crops are food resources for communities of predators and parasitoids. From an agronomic perspective these species are pests and biocontrol agents respectively, and the relationships between them can be important determinants of crop yield and quality. The impact of biocontrol agents on pest populations may depend on the availability of other food resources in the agroecosystem. A scarcity of such resources may limit biological control and altering agroecosystem management to alleviate this limitation could contribute to pest management. This is a tactic of ‘conservation biological control’ and includes the provision of flowers for species that consume prey as larvae but require floral resources in their adult stage. The use of flowers for pest management requires an understanding of the interactions between the flowers, pests, biocontrol agents and non-target species. Without this, attempts to enhance biological control might be ineffective or detrimental. This thesis develops our understanding in two areas which have received relatively little attention: the role of flowers in biological control by true omnivores, and the implications of flower use by fourth-trophic-level life-history omnivores. The species studied were the lacewing Micromus tasmaniae and its parasitoid Anacharis zealandica. Buckwheat flowers Fagopyrum esculentum provided floral resources and aphids Acyrthosiphon pisum served as prey. Laboratory experiments with M. tasmaniae demonstrated that although prey were required for reproduction, providing flowers increased survival and oviposition when prey abundance was low. Flowers also decreased prey consumption by the adult lacewings. These experiments therefore revealed the potential for flowers to either enhance or disrupt biological control by M. tasmaniae. Adult M. tasmaniae were collected from a crop containing a strip of flowers. Analyses to determine the presence of prey and pollen in their digestive tracts suggested that predation was more frequent than foraging in flowers. It was concluded that the flower strip probably did not affect biological control by lacewings in that field, but flowers could be significant in other situations. The lifetime fecundity of A. zealandica was greatly increased by the presence of flowers in the laboratory. Providing flowers therefore has the potential to increase parasitism of M. tasmaniae and so disrupt biological control. A. zealandica was also studied in a crop containing a flower strip. Rubidium-marking was used to investigate nectar-feeding and dispersal from the flowers. In addition, the parasitoids’ sugar compositions were determined by HPLC and used to infer feeding histories. Although further work is required to develop the use of these techniques in this system, the results suggested that A. zealandica did not exploit the flower strip. The sugar profiles suggested that honeydew had been consumed by many of the parasitoids. A simulation model was developed to explore the dynamics of aphid, lacewing and parasitoid populations with and without flowers. This suggested that if M. tasmaniae and A. zealandica responded to flowers as in the laboratory, flowers would only have a small effect on biological control within a single period of a lucerne cutting cycle. When parasitoids were present, the direct beneficial effect of flowers on the lacewing population was outweighed by increased parasitism, reducing the potential for biological control in future crops. The results presented in this thesis exemplify the complex interactions that may occur as a consequence of providing floral resources in agroecosystems and re-affirm the need for agroecology to inform the development of sustainable pest management techniques.