Made available in DSpace on 2014-06-11T19:27:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-02-25Bitstream added on 2014-06-13T20:56:20Z : No. of bitstreams: 1 barbosa_mmc_me_jabo.pdf: 650496 bytes, checksum: d36cf123ceb16765bef9266b5c12dc6e (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Pesquisou-se a ocorrência de E. coli (EPEC, EIEC, EHEC) em água e peixe (pele, trato gastrintestinal e músculo) de pesque-pagues da microbacia do Córrego Rico, Jaboticabal, SP. A amostragem foi realizada entre os meses de abril e junho de 2008 em cinco pesque-pagues. Em cada um, foram colhidos dez exemplares de peixes adultos, e para análise foram colhidas amostras do músculo, do trato gastrintestinal e da superfície corpórea. As amostras de água foram colhidas em cinco pontos distintos de todos os pesque-pague. As colônias com características morfológicas relativas à E. coli isoladas do ágar MacConkey foram submetidas a confirmação bioquímica. Posteriormente, os isolados foram identificados sorologicamente como EPEC, EIEC e EHEC e realizado os testes de susceptibilidade a antimicrobianos e atividade hemolítica. Assim, foram isoladas 115 cepas de E. coli, entre as quais 81 (70%) foram sorogrupadas como EPEC, 5 (4%) como EHEC e 8 (7%) como EIEC. Os sorogrupos mais frenquentes foram O125 (13,9%), O126 (11,3%) e O158 (10,4%). Dentre as amostras testadas, 60 (52%) apresentaram resistência simultânea a dois antimicrobianos. A Análise de Correspondência foi realizada com intuito de verificar as possíveis correspondências envolvendo o local de isolamento, sorogrupos e multirresistência e com isso pode-se observar que o músculo foi o local de isolamento com menor correlação aos demais fatores. Enquanto água, trato e pele apresentaram maior correspondência entre si. Assim, a presença de um alto número de isolados EPEC nesse estudo indica contaminação por enteropatógenos possivelmente advindos de fezes de animais, humanas e/ou águas contaminadas, representando risco à saúde dos frequentadores destes pesque-pagues e consumidores do pescado. / The occurrence of Escherichia coli (EPEC, EIEC, EHEC) in water and fish (skin, gastrointestinal tract and muscle) was surveyed in fee fishing from the stream Rico micro-basin, Jaboticabal, São Paulo/Brazil. Samples were collected between april and june 2008 in five fee fishing. In each fee fishing, ten copies were collected from adult fish and for analysis were sampled muscle, gastrointestinal tract and body surface. Water samples were collected from five different points of all fee fishing. Thus, we isolated colonies from the MacConkey agar with morphological characteristics related to E. coli for biochemical confirmation. Subsequently, the isolates were identified serologically as EPEC, EIEC and EHEC and performed tests of antimicrobial susceptibility and hemolytic activity. It was found that among 115 strains of E. coli isolated, 81 (70%) were serologically classified as EPEC, 5 (4%) as EHEC and 8 (7%) as EIEC. The most common serogroups were O125 (13.9%), O126 (11.3%) e O158 (10.4%). From the 115 bacterial isolates, 60 (52%) were resistant to two antimicrobials. The Correspondence Analysis was performed to verify possible matches involving the location of isolation, serogroup and antimicrobial resistance and that can be observed that the muscle had lower correlation to other factors. While water and tract and skin had correlation with each other. Probably, the isolation of EPEC serogroups in this study indicates contamination by pathogens coming from animals, human and/or contaminated water, constituting a potential risk to the consumer health of these fee fishing.

Made available in DSpace on 2014-06-11T19:22:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-04-09Bitstream added on 2014-06-13T18:08:11Z : No. of bitstreams: 1 lorenzon_cs_me_jabo.pdf: 230149 bytes, checksum: d9f1485e99a4471e89d6e1d273b98a86 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Universidade Estadual Paulista (UNESP) / O objetivo deste trabalho foi determinar o número de coliformes totais, termotolerantes, Staphylococcus coagulase positivo e a presença de bactéria do gênero Salmonella no músculo, no tecido superficial, no trato gastrintestinal de peixes e na água de cultivo de pesque-pagues situados na microbacia do Córrego Rico, região nordeste do estado de São Paulo. Não foi detectado Staphylococcus coagulase positivo em nenhuma amostra de água e peixe. Na contagem de Staphylococcus sp. a água de enxaguadura da pele apresentou números que variam de 1,0 x 102 a 3,3 x 105 UFC.mL-1; no músculo de 1,0 X 102 a 6,7 x 103 UFC.g-1; no trato gastrintestinal de 1,0 x 103 a 2,9 x 105 UFC.g-\ e na água de cultivo de < 20 a 3,3 x 102 UFC.mL-1. O número mais provável (NMP) de coliformes totais na pele variou de 1,5 x 103 a > 1,1 x 104 NMP.100mL-1; no músculo variou de 2,0 x 10 a> 1,1 x 103 NMP.g-1; no trato gastrintestinal variou de 2,6 x 103 a> 1,1 x 104 NMP.g-1; e na água de cultivo variou de 4,2 x 104 a> 2,4 x 105 NMP.100mL-1. Para coliformes termotolerantes a água de enxaguadura da pele variou de < 3,0 x 10 a 1,4 x 103 NMP.IOOmL-1; no músculo variou de < 3 a 6 NMP.g-1; no trato gastrintestinal variou de 1,2 X 103 a 5,1 x 103 NMP.g-1; e na água de cultivo variou de 3,8 x 102 a 2,0 x 104 NMP.100mL-1. Não houve diferença estatística (P>0,05) entre as populações de microrganismos pesquisados na água, pele e trato gastrintestinal o que reflete a relação direta entre a presença dos microrganismos na água e nesses dois locais analisados. No que se refere à musculatura verifica-se que existe diferença (P<0,05) entre a população de microrganismos na água e na musculatura, sendo na musculatura sempre menor. Foi isolada Salmonella sp. em uma amostra de músculo e em duas amostras de trato gastrintestinal. O pescado pode ser veículo de contaminação cruzada, tendo como... / The aim of this work was to determine the number of total coliforms, thermotolerant, Staphylococcus coagulase positive and the presence of bacteria of the genus Salmonella in the muscle, in the surface tissue, in the gastrointestinal tract of fish and pond water of fee- fishing located in Cón-ego Rico microwatershed, northeast region of São Paulo state. Staphylococcus coagulase positive was not detected in any sample of pond water and fish. In the Staphylococcus sp. counts the surface tis sue ranged from 1,0 x 102 to 3,3 X 105 UFC.mL-1; in the musc1e from 1,0 x 102 to 6,7 x 103 UFC.g-1; in the gastrointestinal tract of fish from 1,0 x 103 to 2,9 X 105 UFC.g-1; and in pond water from < 20 to 3,3 X 102 UFC.mL¬1. The most probable number (NMP) of total coliforms in the surface tissue ranged from 1,5 x 103 to > 1,1 X 104 NMP.100mL-1; in the muscle from 2,0 x 10 to > 1,1 X 103 NMP.g-1; in the gastrointestinal tract offish from de 2,6 x 103 to> 1,1 X 104 NMP.g-1; and in pond water from 4,2 x 104 to > 2,4 X 105 NMP.1 00mL-1 . In thermotolerant coliforms the surface tissue ranged from < 3,0 x 10 to 1,4 X 103 NMP.100mL-1; in the musc1e from < 3 to 6 NMP.g-1; in the gastrointestinal tract of fish from de 1,2 x 103 to 5,1 X 103 NMP.g-1; and in pond water from 3,8 x 102 to 2,0 X 104 NMP.100mL-1. There was no statistical difference (P>0,05) among the studied populations of microorganisms in water, skin and gastrointestinal tract that reflects the relationship between the presence of microorganisms in water and in these two tissues analyzed. Conceming the musc1e there is difference (P<0,05) among the population of microorganisms in water and muscle, in which muscles were always smaller. Salmonella sp. was isolated in a sample of muscle and in two samples of gastrointestinal tract. The fish ean be a vehic1e of cross contamination, and the gastrointestinal tract and skin as a source of microorganisms... (Complete abstract click electronic access below)

Orientador: Luiz Augusto do Amaral / Banca: Laudicéia Giacometti Lopes / Banca: Maria da Glória Buzinaro / Resumo: O objetivo deste trabalho foi determinar o número de coliformes totais, termotolerantes, Staphylococcus coagulase positivo e a presença de bactéria do gênero Salmonella no músculo, no tecido superficial, no trato gastrintestinal de peixes e na água de cultivo de pesque-pagues situados na microbacia do Córrego Rico, região nordeste do estado de São Paulo. Não foi detectado Staphylococcus coagulase positivo em nenhuma amostra de água e peixe. Na contagem de Staphylococcus sp. a água de enxaguadura da pele apresentou números que variam de 1,0 x 102 a 3,3 x 105 UFC.mL-1; no músculo de 1,0 X 102 a 6,7 x 103 UFC.g-1; no trato gastrintestinal de 1,0 x 103 a 2,9 x 105 UFC.g-\ e na água de cultivo de < 20 a 3,3 x 102 UFC.mL-1. O número mais provável (NMP) de coliformes totais na pele variou de 1,5 x 103 a > 1,1 x 104 NMP.100mL-1; no músculo variou de 2,0 x 10 a> 1,1 x 103 NMP.g-1; no trato gastrintestinal variou de 2,6 x 103 a> 1,1 x 104 NMP.g-1; e na água de cultivo variou de 4,2 x 104 a> 2,4 x 105 NMP.100mL-1. Para coliformes termotolerantes a água de enxaguadura da pele variou de < 3,0 x 10 a 1,4 x 103 NMP.IOOmL-1; no músculo variou de < 3 a 6 NMP.g-1; no trato gastrintestinal variou de 1,2 X 103 a 5,1 x 103 NMP.g-1; e na água de cultivo variou de 3,8 x 102 a 2,0 x 104 NMP.100mL-1. Não houve diferença estatística (P>0,05) entre as populações de microrganismos pesquisados na água, pele e trato gastrintestinal o que reflete a relação direta entre a presença dos microrganismos na água e nesses dois locais analisados. No que se refere à musculatura verifica-se que existe diferença (P<0,05) entre a população de microrganismos na água e na musculatura, sendo na musculatura sempre menor. Foi isolada Salmonella sp. em uma amostra de músculo e em duas amostras de trato gastrintestinal. O pescado pode ser veículo de contaminação cruzada, tendo como... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The aim of this work was to determine the number of total coliforms, thermotolerant, Staphylococcus coagulase positive and the presence of bacteria of the genus Salmonella in the muscle, in the surface tissue, in the gastrointestinal tract of fish and pond water of fee- fishing located in Cón-ego Rico microwatershed, northeast region of São Paulo state. Staphylococcus coagulase positive was not detected in any sample of pond water and fish. In the Staphylococcus sp. counts the surface tis sue ranged from 1,0 x 102 to 3,3 X 105 UFC.mL-1; in the musc1e from 1,0 x 102 to 6,7 x 103 UFC.g-1; in the gastrointestinal tract of fish from 1,0 x 103 to 2,9 X 105 UFC.g-1; and in pond water from < 20 to 3,3 X 102 UFC.mL¬1. The most probable number (NMP) of total coliforms in the surface tissue ranged from 1,5 x 103 to > 1,1 X 104 NMP.100mL-1; in the muscle from 2,0 x 10 to > 1,1 X 103 NMP.g-1; in the gastrointestinal tract offish from de 2,6 x 103 to> 1,1 X 104 NMP.g-1; and in pond water from 4,2 x 104 to > 2,4 X 105 NMP.1 00mL-1 . In thermotolerant coliforms the surface tissue ranged from < 3,0 x 10 to 1,4 X 103 NMP.100mL-1; in the musc1e from < 3 to 6 NMP.g-1; in the gastrointestinal tract of fish from de 1,2 x 103 to 5,1 X 103 NMP.g-1; and in pond water from 3,8 x 102 to 2,0 X 104 NMP.100mL-1. There was no statistical difference (P>0,05) among the studied populations of microorganisms in water, skin and gastrointestinal tract that reflects the relationship between the presence of microorganisms in water and in these two tissues analyzed. Conceming the musc1e there is difference (P<0,05) among the population of microorganisms in water and muscle, in which muscles were always smaller. Salmonella sp. was isolated in a sample of muscle and in two samples of gastrointestinal tract. The fish ean be a vehic1e of cross contamination, and the gastrointestinal tract and skin as a source of microorganisms... (Complete abstract click electronic access below) / Mestre

In my dissertation, I examine the role of the audit committee chair in the financial reporting process and test if the change in audit committee chair is associated with changes in audit fees, audit report lag, and audit quality. Motivation for this dissertation comes from the increased attention paid by legislators and regulators in recent years on the role of the audit committee in the financial reporting process. While prior studies have examined diverse issues related to the composition of the audit committee, no prior study has examined the role of the audit committee chair on the oversight of financial reporting, even though the chair of the committee has significant control over the functioning of the committee. In the first essay of my dissertation, I show that audit fees are higher in firms that have a change in the audit committee chair. In the second essay, I examine the association between changes in the audit committee chair and audit report lag. In a changes regression, I find that the change in audit committee is associated with higher audit report lag. The third essay examines the association between changes in audit committee chair and two different measures of audit quality: restatements and abnormal accruals. There is no evidence in support of the argument that changes in audit committee chair is associated with higher quality financial reporting. Overall, the results suggest that the change in audit committee chair has an important impact on the financial reporting process of public companies.

This diploma thesis tries to analyze the system of tertiary education financing in the Czech Republic. First part analyses tertiary education as an economic good. The following part shows the main problems of the current financing system in the Czech Republic and through empirical research and many international comparisons proves the importance of changes. It introduces one way how to solve the current state -- income-contingent repayment schemes -- which can work efficiently in case of properly parameter settings. The last part brings the general principles of the reform of higher education in the Czech Republic.

The Czech Republic has approximately 2 126 MWp of photovoltaic installed power capacity, most of the capacity was installed during the "solar boom" period around year 2010. Directive 2012/19/EU on waste electrical and electronic equipment led to the amendment of Act no. 185/2001 Coll. on Waste and the Decree no. 178/2013 Coll., which set the photovoltaic power plant operator´s obligation to pay a contribution for the module´s future recycling. The current rate for calculating the minimum total amount of the contribution is set at CZK 8.50/kg of module. The current amount of the contribution is a subject to criticism. The diploma thesis analyzes public administrations on issues of environmental protection and tools that may be used to motivate polluters. The thesis proposes a new amount of recycling contribution at the amount of CZK 5.2-7.8/kg. Huge temporal inconsistency is the most dangerous risk in determining the contribution, which may threaten many variables essential for the whole calculation.

The thesis analyses the influence of reimbursement regulatory fee for hospitalization which was introduced in regional hospitals in 2009 in Czech Republic. The difference in difference analysis was used to research the possible relationship between reimbursement of the fee and length of hospitalization in acute care hospitals and in after-care facilities. In acute care the influence was 0,5-1 % of the length and in after-care facilities between 8-12 %. This relationship may be biased because of introduction of DRG system which may decrease the length of stay and may be differently distributed between treatment and control group.

The thesis is focused on fair tuition fees. It tries to explore more profoundly all the possible factors, which could be considered as important during the measuring benefits of higher education. Among these factors we can find p.e. Labour market success rate, unemployment and income level of graduates in employment. The other main theme of this thesis is the current situation concerning the funding of higher education in the Czech Republic and other EU countries. The issue of tuition fee is currently very actual and it is clear that proper financial cost analysis of studies and the possibilities of measuring return on investment in tertiary education are more than important. Therefore I present existing possibilities to measure return on investment and also to the following possibilities how to include and use the unemployment rate of graduates.

This thesis deals with the description and analysis of the system of higher education funding in the Czech Republic and in selected EU countries. First, it focuses on the meaning and conception of the education, then the education is analyzed in the view of the public goods theory. The next section of the thesis characterizes the higher education system, analyses its funding and compares these systems within selected countries. The final part focuses on the presentation and evaluation of prepared changes in the higher education law, then applies gained knowledge and suggests possible changes to the funding system in the Czech Republic.

Despite the fact that most of developed countries are succesful in reducing emissions of pollutants, the problem in many places is still deteriorating air quality situation. Air pollution is a problem also for the Czech Republic, where some regions are more affected than others. Therefore was in this thesis chosen one of them, the area of northern Bohemia and specifically the area of Teplice. The work focuses on pollution caused by local heating device because this pollution is not currently regulated. The aim of this work is to determine the behavior of households for heating by local heating device through a questionnaire survey. Especially was monitored the reaction for potential regulatory tool- the motivational subsidy. From these data was in more detail examined the dependence between the amount of subsidies and net monthly income using the Pearson and Spearman correlation coefficient. Research showed no major statistical context, however, for the first time was held an investigation of multispecies heating in households thanks to which it can be better analyzed the reasons for frequent heating with solid fuels.