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Avaliação do volume pulmonar pela ultra-sonografia em três dimensões em fetos com hérnia diafragmática congênita isolada / Assessment of lung volume by three-dimensional ultrasonography in fetuses with isolated congenital diaphragmatic hérniaRuano, Rodrigo 04 May 2005 (has links)
Introdução: A predição do prognóstico neonatal de fetos com hérnia diafragmática congênita (HDC) é um dos principais desafios na Medicina Fetal. A estimativa do volume pulmonar fetal pela ressonância nuclear magnética (RNM) correlaciona-se significativamente com o prognóstico neonatal. Recentemente, foi introduzida a técnica de rotação da imagem multiplanar (VOCALTM) de ultra-sonografia em três dimensões (US-3D), a qual tornou possível estimar o volume pulmonar fetal em casos com HDC. Objetivos: Estimar o volume pulmonar, pela US-3D através da técnica rotacional, em fetos com HDC isolada e comparar com a curva previamente descrita pelo mesmo autor; correlacionar o volume pulmonar fetal total com o prognóstico neonatal; avaliar a acurácia da técnica de rotação em medir o volume pulmonar fetal; e, avaliar outros marcadores ultra-sonográficos clássicos de prognóstico neonatal. Métodos: Este estudo prospectivo foi realizado no período de fevereiro de 2002 a Outubro de 2003 na Maternidade Necker-Enfants Malades, Université de Paris V-Paris, França, em 30 fetos com HDC entre 23 e 36 semanas de gestação. Cada gestante era submetida a um único exame de US-3D, sendo utilizada a técnica de rotação da imagem multiplanar (VOCALTM) para estimar o volume pulmonar fetal. Oito fetos nasceram pós interrupção terapêutica da gestação de acordo com as leis francesas. O volume pulmonar de cada feto (volume observado) foi comparado com a curva padão de nromalidade descrita previamente, sendo calculada a relação entre volumes observado/esperado, a qual foi comparada com a evolução neonatal. Além disso, foram avaliados outros fatores prognósticos clássicos, como: presença de fígado herniado, gravidade do desvio do mediastino, presença de polihidrâmnio, lado da lesão, relação ventrículo esquerdo/ventrículo direito e a relação área do pulmão contralateral/circunferência cefálica (SPC/CC). Foram comparadas também as variações intra- e inter-operador, e a acurácia da ultra-sonografia em estimar o volume pulmonar fetal após a autópsia dos 8 casos em que a gestação foi interrompida. Resultados: Redução importante do volume pulmonar foi observada em fetos com HDC quando esses valores foram distribuídos na curva padrão de normalidade previamente descrita. A relação entre volumes observado/esperado foi significativamente menor nos 12 casos que evoluíram a óbito (mediana= 0,30; variação= 0,12-0,66) em comparação com os 10 sobreviventes (mediana= 0,40; variação= 0,33-0,66; p= 0,017). Dentre outros fatores prognósticos, apenas a relação SPC/CC associou-se significativamente com os resultados neonatais. A predição de óbito neonatal e de sobrevida foi de 90% (9/10) e 75% (9/12) pelo volume pulmonar estimado pela US-3D e de 80% (8/10) e 66,7% (4/8) pela relação SPC/CC estimada pela US-2D, respectivamente. As variações intra- e inter-operadoras foram respectivamente de 0,78cm³ e 0,41cm³. Boa acurácia do método foi observada no presente estudo (84,86%). Conclusões: Em casos com HDC isolada, a estimativa do volume pulmonar fetal pela US-3D, utilizando a técnica rotacional, correlacionou-se significativamente com a evolução neonatal. A relação SPC/CC também se associou significativamente com os resultados neonatais / Introduction: Predicting neonatal outcome in fetuses with congenital diaphragmatic hernia (CDH) is one of the main challenges in Fetal Medicine. Fetal lung volume estimated by magnetic resonance imaging (MRI) associated significantely with neonatal outcome. Recently, the rotationa technique (VOCALTM) on threedimensional ultrasonography (3DUS) was introduced, which allows estimating fetal lung in fetuses with CDH. Objectives: To assess fetal lung volumes by 3DUS using the rotational technique (VOCALTM) in fetuses with isolated CDH, and to plot these values in the nomogram previously described by the same author; to correlate fetal lung volumes with neonatal outcome in cases with CDH; to evaluate the accuracy of 3DUS in estimating fetal lung volumes; and, to evaluate the classical prognostic factors in cases with CDH. Patients and Methods: From Febuary 2002 to October 2003, a prospective study was conducted in Maternité-Hôpital Necker Enfants Malades - Université de Paris V - France, in which 3D- ultrasonographic lung volumes were estiamted in 30 fetuses with isolated CDH 23 and 36 weeks of gestation. Each case was submitted to 3D-ultrasound examination once and 3Dvolumetric measurements were obstained by the technique of rotation of the multiplanar imaging (VOCALTM). Termination of pregnancy was perfromed in 8 cases according to the French law. Observed lung volume in each fetus with isolated CDH was compared to the expected fetal lung for specific gestational age determinated by the nomogram previously described. The observed/expected fetal lung volume was then calculated for each case and correlated with neonatal outcome. Besides,other classical sonographic prognostic were evaluated such as: hydramnios, herniated liver, herniated stomach, lung-over- head ratio, severe mediastinal shift and decreased left/right ventricles ratio. Intra- and inter-operator variabilities were also evaluated, as well as the accuracy of 3D- ultrasound in estimating fetal lung volumes. Results: Observed fetal lung volumes were extremely lower in fetuses with CDH when these values were ptotted in the nomogram of fetal lung volume against gestational age. The observed / expected fetal lung volume ratio was significantly downshifted in 12 cases with CDH who died (median: 0.30, range: 0.12-0.66) compared with 10 suvivors (median: 0.40, range: 0.33-0.66, p= 0,017). Among the other prognostic factors, only the LOHR associated significantly with neonatal outcome. Predicting neonatal deaths and neonatal survivals was 90% (9/10) and 75% (9/12) by fetal lung volume on 3DUS whilst it was 80% (8/10) and 66.7% (4/8) on 2DUS, respectivelly. Intra- and inter-variabilities were 0.78cm³ and 0.41cm³ in cases with CDH, respectively. Good accuracy of this method in estimating fetal lung volume was observed (84.86%). Conclusions: In cases with isolated CDH, fetal lung volume estimated by 3D-ultrasonography using the rotational technique corretated significantly with neonatal outcome. LOHR also associated significantly with neonatal outcome
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Characterizing the spectrum of chromosome copy number variants among fetuses with increased nuchal translucency and normal karyotype by chromosome microarray analysis.January 2014 (has links)
目前廣泛應用于胎兒醫學的唐氏綜合症篩查法,即結合早孕期胎兒頸項透明層的超聲檢查,及母體血清生化指標的綜合篩查法。頸項透明層是指在早孕期利用超聲檢測到的胎兒頸后的皮下積水,其作為預測胎兒異常的一項重要“軟指標,其臨床意義,尤其是與胎兒染色體異常及器官結構異常之間的關係,逐漸得到深入的認識,但其形成機制尚未明確。現在已知有一百餘種畸形及遺傳綜合征與胎兒頸項透明層增厚相關,但其染色體異常譜系,尤其是亞顯微的染色體異常仍有待明確。大部分頸項透明層增厚但核型正常的胎兒預後良好,但約3-10%的這部分胎兒會伴有畸形或出生后的神經智力發育缺陷。而傳統核型分析無法檢測到亞顯微的染色體異常,從而無法判斷這部分核型正常卻伴有缺陷的胎兒是否因為這類染色體異常而致病。 / 微陣列比較基因組雜交芯片作為檢測兒童發育遲緩者及器官結構異常原因的重要手段已廣泛應用于臨床。在染色體核型正常的胎兒中,若伴有器官結構異常的胎兒,5-12%被檢出與該畸形相關的微缺失及微重複;若僅伴有孕婦高齡或唐氏篩查高危,則微缺失及微重複檢出率約1%。 / 該課題旨在研究頸項透明層增厚但核型正常的胎兒中,染色體拷貝數變異發生的頻率及頻譜;評估微陣列比較基因組雜交芯片在協助臨床判斷胎兒預後中的作用。因此,我們開展該多中心隊列研究,通過納入449例頸項透明層厚度≧3.5 mm但正常核型胎兒的,檢測其染色體拷貝數變異,監測并記錄其圍產、產後及新生兒期情況。微陣列比較基因組雜交芯片總共檢出2.8%的異常拷貝數變異,其大小範圍為0.1 kb至18Mb。在伴有器官結構異常的胎兒組中,異常拷貝數變異檢出率達7.8%。對於頸項透明層厚度≧4.0 mm的胎兒,異常拷貝數變異檢出率可達7.3%。 / 對於頸項透明層增厚的胎兒,致病拷貝數變異暫未發現特定的頻譜。但,該研究中發現重複的致病拷貝數變異,如22號染色體長臂1區1帶的微重複或微缺失,2號染色體長臂2區2帶的微缺失。未在3號、7號、12號、13號、18號、20號、21號或Y染色體上發現與胎兒頸項透明層增厚相關的致病拷貝數變異。 / 頸項透明層增厚的胎兒79.3%預後良好;若經微陣列比較基因組雜交芯片未檢出致病拷貝數變異,則81.2%預後良好。如果僅頸項透明層增厚不伴有結構異常的胎兒,經微陣列比較基因組雜交芯片未檢出致病拷貝數變異,則93.5%預後良好。 / 綜上所述,微陣列比較基因組雜交芯片顯著提高了致病拷貝數變異的檢出率。可考慮將微陣列比較基因組雜交芯片作為頸項透明層厚度≧4.0 mm的胎兒染色體異常檢查的首要方法。對於僅頸項透明層增厚不伴有結構異常的胎兒,且經微陣列比較基因組雜交芯片未檢出致病拷貝數變異,絶大部分預後良好。對於頸項透明層增厚的胎兒,致病拷貝數變異暫未發現特定的頻譜,但發現重複出現的致病拷貝數變異。通過初步的基因本體分析及基因通路分析,神經嵴細胞的分化遷徙功能異常可作為今後研究頸項透明層增厚的病理生理機制的方向。 / Measurement of nuchal translucency (NT) has been recognized as a sensitive marker for fetal chromosomal disorders for more than a decade, and is presently used as a routine first-trimester screening test. Although over 100 abnormalities and genetic syndromes have been reported to be associated with increased NT, these associations have not been fully explored and the relevant spectrum of associated submicroscopic chromosomal abnormalities has not been sufficiently investigated. The majority of euploid fetuses with increased NT have a good outcome, but around 3-10% of fetuses present with structural or neurodevelopmental abnormalities postnatally. A range of genetic syndromes has been reported, many of which are linked to submicroscopic chromosomal abnormalities that are typically missed by conventional karyotyping. / Microarray-based comparative genomic hybridization (arrayCGH) has been applied as the first-tier diagnostic tool for the evaluation of developmental delay and structural malformations in children. In fetuses with a normal karyotype, microarray analysis revealed clinically relevant deletions or duplications in 5-12% with a structural anomaly and in about 1% of those whose indications were advanced maternal age or positive screening results. / The objectives of this study were to delineate the frequency and spectrum of pathogenic chromosome copy number variants (CNVs) among fetuses with increased NT and normal karyotype; to evaluate the role of arrayCGH to predict the prognosis of the high NT fetuses; to explore the genotype-phenotype correlations of increased NT. Therefore, a multi-centre cohort of 449 fetuses with NT ≧3.5 mm and normal karyotype were further investigated by arrayCGH. Antenatal surveillance, pregnancy outcome and paediatric follow up were documented. ArrayCGH detected abnormal CNVs in 2.8% (14 of 449) of the fetuses with high NT; the size of CNVs ranged from 0.1 kb to 18Mb. Among fetuses with major congenital abnormalities the incidence of abnormal CNV reached 7.8% (4 of 51). By adjusting the NT to ≧4.0 mm as the referral indication, 7.3% (14 of 192) of the fetuses would have abnormal arrayCGH results. The spectrum of pathogenic CNVs found associated with increased NT was diverse. However, there were recurrent ones such as the deletions or duplications at chromosomal region 22q11, and deletions in ZEB2. There was no pathogenic CNV related with increased NT found in chromosomes 3, 7, 12, 13, 18, 20, 21, or Y. The total normal outcome rate of euploid fetuses with an increased NT was 79.3%; for fetuses with normal arrayCGH results 81.2% had a normal outcome. In fetuses with isolated increased NT, normal arrayCGH results predict a favorable prognosis of 93.5%. / In conclusion, arrayCGH significantly increased the diagnostic yield of pathogenic CNVs. In clinical practice arrayCGH may be considered as the first tier investigation in fetuses with an increased NT more than 4.0 mm. In cases with an isolated increased NT with normal arrayCGH results the pregnancy outcome is likely to be favorable. The spectrum of abnormal CNVs found by arrayCGH is diverse but there are recurrent cases such as del/dup 22q11 and del ZEB2. Our preliminary gene ontology and pathway analysis showed that gene pathways related to neural crest cells may be considered as a future study for physiopathologic mechanisms of NT. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Huang, Jin. / Thesis (Ph.D.) Chinese University of Hong Kong, 2014. / Includes bibliographical references (leaves 106-120). / Abstracts also in Chinese.
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Dysregulation of retinoic acid synthesis in mouse embryos under diabetic or hyperglycemic conditions.January 2011 (has links)
Chan, Wing Lung. / Thesis (M.D.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 111-130). / Abstracts in English and Chinese. / Title --- p.i / Acknowledgements --- p.ii / Table of Content --- p.iii / List of Tables --- p.viii / List of Figures --- p.xi / List of Graphs --- p.xii / Abbreviations --- p.xiv / Abstract --- p.xv / Abstract (Chinese) --- p.xvii / Chapter Chapter 1: --- General Introduction / Chapter 1.1 --- Diabetes Mellitus --- p.2 / Chapter 1.1.1 --- Type 1 diabetes mellitus --- p.3 / Chapter 1.1.2 --- Type 2 diabetes mellitus --- p.4 / Chapter 1.1.3 --- Gestational diabetes mellitus --- p.5 / Chapter 1.2 --- Diabetic Pregnancy --- p.6 / Chapter 1.2.1 --- Incidence of congenital malformations in diabetic pregnancy --- p.6 / Chapter 1.2.2 --- Long term complications in the infant of diabetic mother --- p.7 / Chapter 1.3 --- Hyperglycemia --- p.7 / Chapter 1.4 --- Oxidative Stress --- p.8 / Chapter 1.4.1 --- Oxidative stress and antioxidant enzymes --- p.8 / Chapter 1.4.2 --- Cellular function of oxidative stress --- p.9 / Chapter 1.4.3 --- Adverse effects of excess oxidative stress during embryogenesis --- p.9 / Chapter 1.5 --- Retinoic Acid --- p.10 / Chapter 1.5.1 --- Function of RA during embryonic development --- p.10 / Chapter 1.5.2 --- RA synthesis and degradation --- p.10 / Chapter 1.5.3 --- Mechanisms of retinoic acid signaling : --- p.12 / Chapter 1.5.4 --- Developmental genes regulated by RA --- p.12 / Chapter 1.6 --- Strategy of the Thesis --- p.14 / Chapter Chapter 2: --- General Materials and Methods / Chapter 2.1 --- Animals --- p.17 / Chapter 2.2 --- Induction of Diabetes --- p.17 / Chapter 2.3 --- Mating Methods --- p.18 / Chapter 2.3.1 --- Mice --- p.18 / Chapter 2.3.2 --- Rats --- p.18 / Chapter 2.4 --- Whole Mount In Situ Hybridization --- p.19 / Chapter 2.4.1 --- Synthesis of DNA plasmids and riboprobes --- p.19 / Chapter 2.4.1.1 --- Mini-scale preparation of plasmid DNA --- p.19 / Chapter 2.4.1.2 --- Linearization of DNA plasmid --- p.20 / Chapter 2.4.1.3 --- In vitro transcription and labeling --- p.21 / Chapter 2.4.2 --- Fixation and dehydration of embryos --- p.22 / Chapter 2.4.3 --- Hybridization with RNA probes --- p.23 / Chapter 2.4.4 --- Post-hybridization wash --- p.24 / Chapter 2.4.4.1 --- Pre-absorption of anti-DIG antibody --- p.25 / Chapter 2.4.4.2 --- Embryo powder preparation --- p.25 / Chapter 2.4.5 --- Post antibody wash and signal development --- p.25 / Chapter 2.5 --- Real-time Quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) --- p.26 / Chapter 2.5.1 --- Sample collection and storage --- p.26 / Chapter 2.5.2 --- Total RNA extraction --- p.27 / Chapter 2.5.3 --- Reverse transcription --- p.28 / Chapter 2.5.4 --- Quantitative real-time PCR --- p.28 / Chapter 2.5.5 --- Preparation of cDNA standards for real-time PCR --- p.29 / Chapter 2.6 --- RA-responsive Cell Line --- p.29 / Chapter 2.6.1 --- Cell culture --- p.30 / Chapter 2.6.2 --- Seeding 96-well plate with RA-responsive cells --- p.31 / Chapter 2.6.3 --- Applying samples to 96-well plate coated with RA-responsive cells --- p.31 / Chapter 2.6.4 --- β-galactosidase staining --- p.32 / Chapter 2.7 --- Separation of Protein Isoforms by Isoelectric Focusing (IEF) --- p.33 / Chapter 2.7.1 --- Preparing protein samples for IEF --- p.33 / Chapter 2.7.2 --- Isoelectric focusing --- p.33 / Chapter 2.7.3 --- IEF native gel staining --- p.34 / Chapter 2.7.4 --- Locating three retinaldehyde dehydrogenase (Raldh) isoforms --- p.35 / Chapter 2.8 --- In Vitro RA Synthesizing Reaction --- p.36 / Chapter Chapter 3: --- Effect of Maternal Diabetes on Retinoic Acid Synthesis in the Mouse Embryo / Chapter 3.1 --- Introduction --- p.38 / Chapter 3.2 --- Experimental Design --- p.41 / Chapter 3.3 --- Materials and Methods --- p.42 / Chapter 3.3.1 --- Sample collection --- p.42 / Chapter 3.3.1.1 --- Criteria for selecting embryos at the same developmental stage --- p.42 / Chapter 3.3.1.2 --- Sample collection for in situ hybridization --- p.42 / Chapter 3.3.1.3 --- Sample collection for real-time quantitative RT-PCR --- p.43 / Chapter 3.3.1.4 --- Sample collection for in vitro RA synthesizing reaction --- p.44 / Chapter 3.3.2 --- Statistical analyses --- p.45 / Chapter 3.4 --- Results --- p.46 / Chapter 3.4.1 --- "Comparison of the in situ expression pattern of Raldh 1, Raldh2 and Raldh3 between embryos of diabetic and non-diabetic mice" --- p.46 / Chapter 3.4.1.1 --- In situ hybridization patterns of Raldh 1 --- p.46 / Chapter 3.4.1.2 --- In situ hybridization patterns of Raldhl --- p.46 / Chapter 3.4.1.3 --- In situ hybridization patterns of Raldh3 --- p.47 / Chapter 3.4.2 --- "Comparison of the relative expression level of Raldh 1, Raldh2 and Raldh3 between embryos of diabetic and non-diabetic mice at different developmental stages" --- p.48 / Chapter 3.4.2.1 --- Relative expression levels of Raldh 1 --- p.50 / Chapter 3.4.2.2 --- Relative expression levels of Raldh2 --- p.50 / Chapter 3.4.2.3 --- Relative expression levels of Raldh3 --- p.51 / Chapter 3.4.3 --- Comparison of the in vitro RA synthesizing activity of Raldh 1 Raldh2 and Raldh3 enzymes between embryos of diabetic and non-diabetic mice at different developmental stages --- p.52 / Chapter 3.5 --- Discussion --- p.55 / Chapter Chapter 4: --- Effect of Hyperglycemia on Retinoic Acid Synthesis / Chapter 4.1 --- Introduction --- p.59 / Chapter 4.2 --- Experimental Design --- p.61 / Chapter 4.3 --- Materials and Methods --- p.64 / Chapter 4.3.1 --- Phlorizin treatment --- p.64 / Chapter 4.3.2 --- Whole rat embryo culture --- p.64 / Chapter 4.3.3 --- Preparation of rat serum --- p.65 / Chapter 4.3.4 --- In situ hybridization --- p.66 / Chapter 4.3.5 --- Real-time quantitative RT-PCR --- p.66 / Chapter 4.3.6 --- In vitro RA synthesizing reaction --- p.68 / Chapter 4.3.7 --- Statistical analyses --- p.68 / Chapter 4.4 --- Results --- p.70 / Chapter 4.4.1 --- "Comparison of the relative expression level of Raldh 1, Raldh2 and Raldh3 between embryos of diabetic and non-diabetic mice injected with phlorizin or suspension vehicle as control" --- p.70 / Chapter 4.4.2 --- Comparison of the in vitro RA synthesizing activity of different isoforms of Raldh enzymes between embryos of diabetic and non-diabetic mice injected with phlorizin or suspension vehicle as control --- p.73 / Chapter 4.4.3 --- In situ expression pattern of Raldh2 in rat embryos cultured in medium containing varying concentrations of D-glucose --- p.77 / Chapter 4.4.4 --- Relative expression levels of Raldh2 in rat embryos cultured in medium supplemented with varying concentrations of D-glucose --- p.78 / Chapter 4.4.5 --- In vitro RA synthesizing activity ofRaldh2 in rat embryos cultured in medium supplemented with varying concentrations of D-glucose --- p.79 / Chapter 4.5 --- Discussion : --- p.82 / Chapter Chapter 5: --- In Vitro Supplementation with RA Rescued Rat Embryos from Hyperglycemia-induced Congenital Malformations / Chapter 5.1 --- Introduction --- p.86 / Chapter 5.2 --- Experimental Design --- p.88 / Chapter 5.3 --- Materials and Methods --- p.89 / Chapter 5.3.1 --- Preparation of RA --- p.89 / Chapter 5.3.2 --- Supplementation of RA to rat embryos in culture --- p.89 / Chapter 5.3.3 --- Morphological scoring system --- p.90 / Chapter 5.3.4 --- Statistical analyses --- p.90 / Chapter 5.4 --- Results --- p.92 / Chapter 5.4.1 --- Supplementation with RA rescued embryos from hyperglyce- miainduced malformations --- p.92 / Chapter 5.5 --- Discussion --- p.101 / Chapter Chapter 6: --- Conclusion and Future Perspectives / Chapter 6.1 --- Conclusion and Future Perspectives --- p.106 / References --- p.111
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Avaliação do volume pulmonar pela ultra-sonografia em três dimensões em fetos com hérnia diafragmática congênita isolada / Assessment of lung volume by three-dimensional ultrasonography in fetuses with isolated congenital diaphragmatic hérniaRodrigo Ruano 04 May 2005 (has links)
Introdução: A predição do prognóstico neonatal de fetos com hérnia diafragmática congênita (HDC) é um dos principais desafios na Medicina Fetal. A estimativa do volume pulmonar fetal pela ressonância nuclear magnética (RNM) correlaciona-se significativamente com o prognóstico neonatal. Recentemente, foi introduzida a técnica de rotação da imagem multiplanar (VOCALTM) de ultra-sonografia em três dimensões (US-3D), a qual tornou possível estimar o volume pulmonar fetal em casos com HDC. Objetivos: Estimar o volume pulmonar, pela US-3D através da técnica rotacional, em fetos com HDC isolada e comparar com a curva previamente descrita pelo mesmo autor; correlacionar o volume pulmonar fetal total com o prognóstico neonatal; avaliar a acurácia da técnica de rotação em medir o volume pulmonar fetal; e, avaliar outros marcadores ultra-sonográficos clássicos de prognóstico neonatal. Métodos: Este estudo prospectivo foi realizado no período de fevereiro de 2002 a Outubro de 2003 na Maternidade Necker-Enfants Malades, Université de Paris V-Paris, França, em 30 fetos com HDC entre 23 e 36 semanas de gestação. Cada gestante era submetida a um único exame de US-3D, sendo utilizada a técnica de rotação da imagem multiplanar (VOCALTM) para estimar o volume pulmonar fetal. Oito fetos nasceram pós interrupção terapêutica da gestação de acordo com as leis francesas. O volume pulmonar de cada feto (volume observado) foi comparado com a curva padão de nromalidade descrita previamente, sendo calculada a relação entre volumes observado/esperado, a qual foi comparada com a evolução neonatal. Além disso, foram avaliados outros fatores prognósticos clássicos, como: presença de fígado herniado, gravidade do desvio do mediastino, presença de polihidrâmnio, lado da lesão, relação ventrículo esquerdo/ventrículo direito e a relação área do pulmão contralateral/circunferência cefálica (SPC/CC). Foram comparadas também as variações intra- e inter-operador, e a acurácia da ultra-sonografia em estimar o volume pulmonar fetal após a autópsia dos 8 casos em que a gestação foi interrompida. Resultados: Redução importante do volume pulmonar foi observada em fetos com HDC quando esses valores foram distribuídos na curva padrão de normalidade previamente descrita. A relação entre volumes observado/esperado foi significativamente menor nos 12 casos que evoluíram a óbito (mediana= 0,30; variação= 0,12-0,66) em comparação com os 10 sobreviventes (mediana= 0,40; variação= 0,33-0,66; p= 0,017). Dentre outros fatores prognósticos, apenas a relação SPC/CC associou-se significativamente com os resultados neonatais. A predição de óbito neonatal e de sobrevida foi de 90% (9/10) e 75% (9/12) pelo volume pulmonar estimado pela US-3D e de 80% (8/10) e 66,7% (4/8) pela relação SPC/CC estimada pela US-2D, respectivamente. As variações intra- e inter-operadoras foram respectivamente de 0,78cm³ e 0,41cm³. Boa acurácia do método foi observada no presente estudo (84,86%). Conclusões: Em casos com HDC isolada, a estimativa do volume pulmonar fetal pela US-3D, utilizando a técnica rotacional, correlacionou-se significativamente com a evolução neonatal. A relação SPC/CC também se associou significativamente com os resultados neonatais / Introduction: Predicting neonatal outcome in fetuses with congenital diaphragmatic hernia (CDH) is one of the main challenges in Fetal Medicine. Fetal lung volume estimated by magnetic resonance imaging (MRI) associated significantely with neonatal outcome. Recently, the rotationa technique (VOCALTM) on threedimensional ultrasonography (3DUS) was introduced, which allows estimating fetal lung in fetuses with CDH. Objectives: To assess fetal lung volumes by 3DUS using the rotational technique (VOCALTM) in fetuses with isolated CDH, and to plot these values in the nomogram previously described by the same author; to correlate fetal lung volumes with neonatal outcome in cases with CDH; to evaluate the accuracy of 3DUS in estimating fetal lung volumes; and, to evaluate the classical prognostic factors in cases with CDH. Patients and Methods: From Febuary 2002 to October 2003, a prospective study was conducted in Maternité-Hôpital Necker Enfants Malades - Université de Paris V - France, in which 3D- ultrasonographic lung volumes were estiamted in 30 fetuses with isolated CDH 23 and 36 weeks of gestation. Each case was submitted to 3D-ultrasound examination once and 3Dvolumetric measurements were obstained by the technique of rotation of the multiplanar imaging (VOCALTM). Termination of pregnancy was perfromed in 8 cases according to the French law. Observed lung volume in each fetus with isolated CDH was compared to the expected fetal lung for specific gestational age determinated by the nomogram previously described. The observed/expected fetal lung volume was then calculated for each case and correlated with neonatal outcome. Besides,other classical sonographic prognostic were evaluated such as: hydramnios, herniated liver, herniated stomach, lung-over- head ratio, severe mediastinal shift and decreased left/right ventricles ratio. Intra- and inter-operator variabilities were also evaluated, as well as the accuracy of 3D- ultrasound in estimating fetal lung volumes. Results: Observed fetal lung volumes were extremely lower in fetuses with CDH when these values were ptotted in the nomogram of fetal lung volume against gestational age. The observed / expected fetal lung volume ratio was significantly downshifted in 12 cases with CDH who died (median: 0.30, range: 0.12-0.66) compared with 10 suvivors (median: 0.40, range: 0.33-0.66, p= 0,017). Among the other prognostic factors, only the LOHR associated significantly with neonatal outcome. Predicting neonatal deaths and neonatal survivals was 90% (9/10) and 75% (9/12) by fetal lung volume on 3DUS whilst it was 80% (8/10) and 66.7% (4/8) on 2DUS, respectivelly. Intra- and inter-variabilities were 0.78cm³ and 0.41cm³ in cases with CDH, respectively. Good accuracy of this method in estimating fetal lung volume was observed (84.86%). Conclusions: In cases with isolated CDH, fetal lung volume estimated by 3D-ultrasonography using the rotational technique corretated significantly with neonatal outcome. LOHR also associated significantly with neonatal outcome
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Systematic chromosome-wide search for novel fetal epigenetic markers for detection of fetal trisomy 13.January 2010 (has links)
Lam, Yuk Man. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 142-157). / Abstracts in English and Chinese. / ABSTRACT --- p.i / 摘要 --- p.iv / ACKNOWLEDGEMENTS --- p.vi / CONTRIBUTORS --- p.viii / PUBLICATIONS --- p.ix / LIST OF TABLES --- p.x / LIST OF FIGURES --- p.xi / LIST OF ABBREVIATIONS --- p.xiii / TABLE OF CONTENTS --- p.xiv / Chapter SECTION I: --- BACKGROUND --- p.1 / Chapter CHAPTER 1: --- PRENATAL DIAGNOSIS OF FETAL ANEUPLOIDIES --- p.2 / Chapter 1.1 --- The need for prenatal screening and diagnosis --- p.2 / Chapter 1.2 --- Patau Syndrome (Trisomy 13) --- p.2 / Chapter 1.3 --- Current methods for fetal aneuploidy detection --- p.4 / Chapter 1.3.1 --- Routine prenatal screening tests --- p.4 / Chapter 1.3.2 --- Definitive prenatal diagnosis by invasive procedures --- p.7 / Chapter 1.4 --- New approach for noninvasive prenatal diagnosis --- p.11 / Chapter 1.4.1 --- Circulating fetal cells --- p.11 / Chapter 1.4.2 --- Cell-free fetal nucleic acids in maternal circulation --- p.12 / Chapter 1.4.3 --- Diagnostic applications of cell-free fetal nucleic acids in maternal plasma --- p.12 / Chapter CHAPTER 2: --- DEVELOPMENT OF FETAL EPIGENETIC MARKERS IN MATERNAL PLASMA --- p.17 / Chapter 2.1 --- Limitations of fetal DNA markers --- p.17 / Chapter 2.2 --- DNA methylation is an actively-researched area under the field of epigenetics --- p.18 / Chapter 2.3 --- Genome-scale DNA methylation analysis brings new insight into epigenetics --- p.20 / Chapter 2.4 --- The first demonstration of using an epigenetic method for detecting maternally-inherited fetal DNA in maternal plasma --- p.22 / Chapter 2.5 --- The first universal marker for fetal DNA in maternal plasma --- p.24 / Chapter 2.6 --- Discovery of more fetal epigenetic markers --- p.25 / Chapter 2.6.1 --- Methylated fetal epigenetic markers are more desirable --- p.25 / Chapter 2.6.2 --- Discovery of hypermethylated fetal epigenetic markers by studying tumor suppressor genes --- p.26 / Chapter 2.6.3 --- Discovery of hypermethylated fetal epigenetic markers on chromosome 21 --- p.28 / Chapter 2.7 --- Noninvasive detection of fetal aneuploidies using fetal epigenetic markers --- p.29 / Chapter 2.7.1 --- Noninvasive detection of fetal trisomy 18 by the epigenetic allelic ratio (EAR) approach --- p.29 / Chapter 2.7.2 --- Noninvasive detection of fetal trisomy 21 by the epigenetic-genetic (EGG) approach --- p.30 / Chapter 2.8 --- Aim of thesis --- p.32 / Chapter SECTION II: --- MATERIALS AND METHODS --- p.34 / Chapter CHAPTER 3: --- METHODS FOR QUANTITATIVE ANALYSIS OF DNA METHYLATION --- p.35 / Chapter 3.1 --- Subject recruitment and sample collection --- p.35 / Chapter 3.2 --- Sample processing --- p.38 / Chapter 3.3 --- DNA extraction --- p.38 / Chapter 3.3.1 --- Placental tissues --- p.38 / Chapter 3.3.2 --- Maternal blood cells --- p.39 / Chapter 3.3.3 --- Maternal plasma --- p.40 / Chapter 3.4 --- Methylated DNA immunoprecipitation and tiling array analysis (MeDIP-chip) --- p.41 / Chapter 3.4.1 --- Principles --- p.41 / Chapter 3.4.2 --- DNA sample and array processing --- p.43 / Chapter 3.4.2.1 --- DNA preparation and target hybridization --- p.43 / Chapter 3.4.2.2 --- Data analysis --- p.44 / Chapter 3.5 --- DNA methylation analysis on randomly-chosen regions on chromosome / Chapter 3.6 --- Bisulfite conversion --- p.46 / Chapter 3.6.1 --- Principles of bisulfite conversion --- p.46 / Chapter 3.6.2 --- Procedures of bisulfite conversion --- p.46 / Chapter 3.7 --- Quantitative analysis of DNA methylation --- p.47 / Chapter 3.7.1 --- Bisulfite PCR and genomic sequencing --- p.47 / Chapter 3.7.1.1 --- Primer design for bisulfite polymerase chain reaction (PCR) --- p.47 / Chapter 3.7.1.2 --- Bisulfite PCR --- p.49 / Chapter 3.7.1.3 --- Cloning --- p.50 / Chapter 3.7.1.4 --- Bisulfite genomic sequencing --- p.52 / Chapter 3.7.1.5 --- Data acquisition and interpretation --- p.53 / Chapter 3.7.2 --- EpiTYPER,a mass-spectrometry-based method --- p.54 / Chapter 3.7.2.1 --- Principles of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) --- p.54 / Chapter 3.7.2.2 --- Primer design of the EpiTYPER assay --- p.55 / Chapter 3.7.2.3 --- The EpiTYPER assay and its principle --- p.56 / Chapter 3.8 --- Methylation-sensitive restriction enzyme (MSRE)-mediated real-time quantitative PCR (qPCR) --- p.61 / Chapter 3.9 --- Digital PCR --- p.66 / Chapter 3.9.1 --- Principles of digital PCR --- p.66 / Chapter 3.9.2 --- Poisson distribution --- p.68 / Chapter 3.10 --- Statistical analyses --- p.69 / Chapter SECTION III: --- SYSTEMATIC IDENTIFICATION OF A FETAL DNA METHYLATION MARKER ON CHROMOSOME 13 FOR DETECTION OF FETAL TRISOMY 13 --- p.70 / Chapter CHAPTER 4: --- SYSTEMATIC IDENTIFICATION OF POTENTIAL FETAL EPIGENETIC MARKERS BY MEDIP-CHIP ANALYSIS --- p.71 / Chapter 4.1 --- Systematic discovery of fetal epigenetic markers on chromosome 13 by MeDIP-chip analysis --- p.71 / Chapter 4.2 --- Experimental design --- p.73 / Chapter 4.3 --- Results --- p.76 / Chapter 4.3.1 --- Identification of differentially methylated DNA regions by MeDIP-chip or non-MeDIP-chip approaches followed by EpiTYPER analysis --- p.76 / Chapter 4.3.2 --- Confirmation of differential methylation patterns and exclusion of regions with high inter-individual variations by EpiTYPER analysis --- p.82 / Chapter 4.3.3 --- Confirmation of differential DNA methylation patterns with higher resolution by bisulfite sequencing --- p.85 / Chapter 4.4 --- Discussion --- p.95 / Chapter CHAPTER 5: --- THE APPLICATION OF FETAL EPIGENETIC MARKER ON CHROMSOME 13 FOR DETECTION OF FETAL TRISOMY 13 --- p.98 / Chapter 5.1 --- Identification of a fetal epigenetic marker on chromosome 13 for the detection of fetal trisomy 13 by the epigenetic-genetic (EGG) chromosome dosage approach --- p.98 / Chapter 5.2 --- Experimental design --- p.101 / Chapter 5.3 --- Results --- p.105 / Chapter 5.3.1 --- Optimization of the digestion protocol --- p.105 / Chapter 5.3.2 --- Detection of digestion-resistant EFNB2-3'UTR moleculesin maternal plasma --- p.109 / Chapter 5.3.3 --- Evaluation of the fetal specificity of digestion-resistant EFNB2´ؤ3 'UTR DNA molecules in maternal plasma --- p.111 / Chapter 5.3.4 --- Comparison of EFNB2-3'UTR methylation profiles between the euploid and trisomy 13 placental tissue samples --- p.115 / Chapter 5.3.5 --- Chromosome dosage analysis by the EGG analysis using placental tissue samples --- p.118 / Chapter 5.4 --- Discussion --- p.122 / Chapter SECTION IV: --- CONCLUDING REMARKS --- p.125 / Chapter CHAPTER 6: --- CONCLUSION AND FUTURE PERSPECTIVES --- p.126 / Chapter 6.1 --- Development of fetal epigenetic markers for noninvasive prenatal diagnosis --- p.126 / Chapter 6.2 --- Systematic identification of fetal epigenetic markers on chromosome13 --- p.127 / Chapter 6.3 --- Detection of fetal trisomy 13 by the epigenetic-genetic (EGG) relative chromosome dosage analysis --- p.129 / Chapter 6.4 --- Future perspectives --- p.132 / Appendix I --- p.134 / Appendix II --- p.136 / REFERENCES --- p.142
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Women's rights? the politics of eugenic abortion in modern Japan /Kato, Masae, January 1900 (has links)
Thesis (doctoral)--Universiteit Leiden, 2005. / Title from e-book title screen (viewed Aug. 10, 2009). Description based on print version record. Includes bibliographical references and index.
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Development of new markers and approaches for the detection of fetal DNA in maternal plasma. / CUHK electronic theses & dissertations collectionJanuary 2008 (has links)
Another attempt was made to identify CpG-rich paralogues on chromosome 21 for dosage analysis. Methylation profiles of 14 paralogous CpG-rich clusters were screened by bisulfite genomic sequencing and/or combined bisulfite restriction analysis. One of the paralogue pairs showed similar differential methylation patterns, and three other CpG-rich clusters located on chromosome 21 were hypomethylated in the placentas and completely methylated in the maternal blood cells. Detection methods for these novel epigenetic markers were described and discussed, and potential applications were also suggested. / In the second part of this thesis, a technique called digital PCR was used for detecting and quantifying cell-free fetal DNA in maternal plasma. DNA templates are first diluted to a single molecule level and partitioned to separate compartments before subjecting to polymerase chain reaction amplification. Quantification is then made by counting the number of positive reactions directly. Such a technique has allowed the reliable detection of fetal DNA from a high background of maternal plasma DNA, and allows absolute quantification of fetal DNA without using a calibration curve. As a proof-of-principle project, a non-polymorphism-based approach called digital relative chromosome dosage (RCD) method was developed to detect chromosomal imbalance in trisomic cases. The implementation of digital PCR was further facilitated with the technology of integrated fluidics circuits (IFCs), by which nanolitre volumes of reaction mixtures could be manipulated in a high-throughput manner. Such a microfluidics digital PCR system was evaluated systematically and shown to be highly accurate, precise and sensitive compared to other existing detection platforms. The technology has been applied with the RCD approach for rapid detection of trisomy 21 from amniotic fluid samples and 100% accuracy was attained. With the development of new universal markers and robust detection platforms, it is envisioned that circulating fetal DNA in maternal plasma can be applied to an expanding range of clinical applications in the near future. / The first part of my thesis focused on the discovery of new epigenetic markers for fetal DNA detection. Methylation profiles of 7 selected CpG islands on chromosome 21 were revealed by bisulfite sequencing, in the hope of identifying regions with differential methylation patterns between placentas and maternal blood cells. Out of the 14 sub-regions of these CpG islands, five displayed significant difference between the two tissue type and were promising marker candidates. / The presence of circulating fetal DNA in maternal plasma provides a non-invasive source of fetal genetic materials for prenatal diagnosis. Reliance on Y-chromosomal sequences for detecting fetal-specific signals from the background of maternal plasma DNA, however, has restricted the applications to 50% of pregnancies. For a wider extent of diagnostic applications, sex- and polymorphism-independent fetal markers would be necessary. Recently, an epigenetic approach has been adopted to discriminate between the fetal and maternal DNA circulating in maternal plasma. Based on the differential DNA methylation status between the fetus and mother, universal fetal DNA markers have been developed and applied to detect fetal signals in maternal plasma. Identification of more of such markers is important for the development of the field. / Lun, Miu Fan. / Adviser: Yuk Ming Dennis Lo. / Source: Dissertation Abstracts International, Volume: 70-06, Section: B, page: 3292. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 233-256). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstracts in English and Chinese. / School code: 1307.
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The effects of maternal smoking on infant immune developmentNoakes, Paul Stanton January 2006 (has links)
[Truncated abstract] With the dramatic rise in asthma and allergic disease there is an urgent need to define the early life exposures which influence developing immune responses to increase the predisposition to allergic disease. While this is clearly multifactorial, this thesis addresses the effects of maternal smoking as a major adverse, yet avoidable exposure in early life. I hypothesised that the well-documented increased susceptibility to infection in infants of smokers could indicate underlying effects on innate Toll-like receptor (TLR) mediated microbial responses which could in turn contribute to early immune dysregulation and increased risk of allergic disease. In addition to providing the first defence against microbes, TLR-mediated pathways modulate subsequent specific immune response and are of growing interest in the potential inhibition of inappropriate allergic responses. My initial interest in the potential immune effects of smoking in pregnancy was based on preliminary retrospective analyses of a previous cohort (presented in Chapter 3) which suggested possible effects on T cell cytokine responses to mitogens and allergens. Based on this, I recruited a new prospective pregnancy cohort (n=122) of smokers (n=60) and non-smokers (n=62) (as outlined in Chapter 4) to confirm this and test my novel hypothesis that maternal smoking may be affecting important innate (TLR-mediated) immune pathways. … Thus, these findings could indicate that smoking increases the early susceptibility to infection thereby increasing subsequent IgA responses. This is supported by observations that key neonatal TLR responses are attenuated in children who go onto develop wheezy illnesses and lower respiratory tract infections. Together, the study findings suggest that in addition to effects on lung growth, maternal smoking may also influence aspects of neonatal innate immune function that are now believed to play a critical role in microbial-driven postnatal immune development, highlighting that other environmental interactions are also highly relevant to the v
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An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiramHeiberg, Ludvig January 1990 (has links)
Although there have been two-dimensional electrophoretic studies on fetal brain tissue (for instance, Yoshida and Takahashi, 1980), the emphasis in most of this work has been on developmental changes in protein expression, and not on the effects that drugs have on fetal brain protein complement. Klose and co-workers (1977) did an early study using two-dimensional gel electrophoresis to determine the effects of various teratogens on whole embryos. No protein changes were found and that line of research was not continued. In this study two-dimensional gel electrophoresis is extensively used, in the belief that the usefulness of this technique to experimental teratology has not been fully evaluated. It is reasonable to suppose that a central nervous system teratogen administered during critical periods of susceptibility will led to perturbations of orderly brain development, and that these perturbations will be reflected as changes to the protein complement. The total brain protein complement of mice that have been exposed to drugs in utero will therefore be analysed, in the hope that any inductions or deletions of proteins as a result of drug exposure may provide a clue to the molecular events underlying drug injury to the fetus.
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Le statut juridique du foetus: analyse rhétoriqueRolland, Louise January 1992 (has links)
Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.
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