Sugar intake is linked to developmental programming of obesity and diabetes. We hypothesized that supplementing ground beef in place of sugar during pregnancy would reduce fetal and offspring developmental programming. Gestating sows were fed 1 of 4 isocaloric supplements: control, ground beef, granulated sugar, or beef plus sugar. In the fetal study supplements were fed from d 40 to 110 of gestation and in the offspring study from d 40 until weaning. Gene expression differences in fetal liver and muscle were observed for IGF2 (P = 0.04), FBPase (P = 0.03), and IGF2R (P = 0.02). Differences were also seen in offspring back fat (sex by day interaction, P = 0.01), longissimus dorsi muscle area (treatment by sex, P = 0.001), body weight (sex, P = 0.0006; sex by day interaction, P < 0.0001), and plasma insulin concentrations (treatment by sex, P = 0.0002). / North Dakota Beef Commission / Topigs Norsvin / North Dakota. State Board of Agricultural Research and Education

No description available.

O pelo é uma característica dos mamíferos: na maioria das espécies, juntamente com a pele, a pelagem reveste externamente o corpo, com exceção de algumas regiões bem delimitadas. O pelo do cão e demais mamíferos apresenta formato distinto de acordo com a região do corpo. O estudo da obtenção e caracterização de células-tronco em cães poderá ampliar as possibilidades terapêuticas, mediante a utilização das células indiferenciadas na reparação capilar e lesões da pele e seus anexos. Neste trabalho foram utilizadas amostras de folículo piloso retiradas da região rostral de fetos caninos correspondentes a três fases gestacionais distintas (grupo I= 30 dias de gestação; grupo II= 35 dias de gestação; grupo III= 40 dias de gestação).Estas amostras foram cultivadas em placas de Petri de 75 cm² com 5mL de meio de cultivo DMEM, suplementado com 10% de soro fetal bovino (SFB) e 1% de antibióticos estreptomicina/penicilina. As células obtidas foram congeladas e descongeladas e utilizados nos procedimentos de, análise da expressão dos marcadores de células-tronco: STRO-1, CD 117, OCT 3/4, CD 90, Nanog, CD- 34, SSEA-4, CD -105, MCP-1, HSP- 47, CD 1 A, VEGFR1, DR4, IL- 1 &beta;, caspase3, Ki- 67, CD -45. A escolha dos anticorpos marcadores de células-tronco foi baseada em estudos que mostraram afinidade e avidez com o folículo piloso. Utilizamos também para a caracterização do folículo piloso, a imunohistoquímica e análise de PCR com o marcador S100 e a imunocitoquímica com os marcadores OCT 3/4, VEGF, STRO-1, CD117 e Nanog. Os resultados obtidos sugerem que a linhagem celular do folículo piloso de fetos de cães apresentaram crescimento satisfatório com a utilização do meio DMEM-hight suplementado com 10% de SFB inativado e 1% de antibióticos, sendo mantidas e expandidas em cultivo. O crescimento e a expansão das células-tronco do folículo piloso são peculiares, pois, sua expansão ocorre em torno da haste pilosa, utilizando-a como ancoragem. Os resultados indicaram características de pluripotência nas células-tronco do folículo piloso pela expressão dos marcadores OCT3/4, Nanog, CD105, CD90, SSEA-4, e STRO-1, em todos os grupos analisados. Considerando, a taxa de proliferação, a fase do ciclo celular, distribuição e aspectos de morte celular não foram encontradas diferenças significantes na expressão dos marcadores HSP47, DR4. Para verificar a angiogêneseforam utilizados os marcadores VEGFR1, Ki-67 e caspase-3, sendo expressos em todos os grupos. Na imunohistoquímica e análise de PCR observou-se a expressão da proteína S100, sendo maior nas células do grupo de fetos com 40 dias de gestação- grupo III. Neste estudo, podemos concluir que o conjunto de marcadores expressos nos diferentes grupos de células tronco obtidas das vibrissas de fetos caninos indicaram a expressão de marcadores de pluripotência das células-tronco do folículo piloso / Hair is a characteristic of mammals, since in most species the coat spreads throughout the body, except for some well-defined regions. Mammals in general, including dogs, have different characteristics in their coat according to the region of the body. The study of obtainment and characterization of stem cells from the hair follicle could contribute to new therapeutic possibilities, particularly in the treatment of hair, skin and it appendices injuries. In this study, hair follicle samples were harvestedin the rostral region of canine fetuses, divided into three distinct groups, according to the stage of pregnancy, as follows: Group I = 30 days, Group II = 35 days, Group III = 40 days. These samples were grown in 75 cm² Petri dishes with 5 mL of DMEM culture medium supplemented with 10% fetal bovine serum (FBS) and antibiotics 1% streptomycin / penicillin. Cells were frozen and thawed and used in procedures for analysis of expression of markers of stem cells: STRO-1, CD 117, OCT 3/4, CD 90, Nanog, CD- 34,SSEA -4,CD- 105, MCP-1, HSP-47, CD 1 A, VEGF-R1, DR4, IL-1 &beta;, caspase-3, Ki-67, CD -45. The choice of antibodies was based on studies that showed affinity and avidity to the hair follicle. In addition, for purposes of characterization of the hair follicle, procedures for immunohistochemistry and PCR analysis with the marker S100 and immunocytochemistry with the markers OCT3/4, VEGF, STRO-1, CD117 e Nanog were used. The results suggested that hair follicle cell line of fetal dogs showed satisfactory growth using the DMEM high medium supplemented with 10% inactivated FBS and 1% antibiotics and could be maintained and expanded in culture. The growth and expansion of stem cells of the hair follicle are unique because they occur around the hair shaft using it as anchorage. Results also indicated pluripotency features in the hair follicle stem cells through the positive expression of Oct3/4, Nanog, CD105, CD90, SSEA-4 and STRO-1, in all groups. Regarding the proliferation rate and cell cycle phase, distribution and cell death aspects, HSP47, DR4 were used. To verify angiogenesis, VEGFR1, Ki67 and Caspase-3 were expressed in all groups. In Immunohistochemistry procedures we observed the expression of S100, and in mRNA on RT-PCR. We concluded in this study that in group III the expression of S100 was higher than in the two other groups. In addition, we found that the pluripotency of the stem cells was indicated by the expression of the markers OCT3/4, Nanog, CD105, CD90, SSEA-4 and STRO-1

Made available in DSpace on 2016-04-04T18:27:32Z (GMT). No. of bitstreams: 1 ELIANE VERGINIA ROVIGATTI.pdf: 459682 bytes, checksum: 4ef72e4608cb8e79b5cb94e8f09e9fa5 (MD5) Previous issue date: 1999-02-26 / Pontif?cia Universidade Cat?lica de Campinas / The present study reports the emotional experiences of 4 couples after being informed of severe anomalies impairing their unborn fetuses. This study aimed at: 1) investigating the emotional reactions, that is, fantasies, anguishes, and defenses triggered in these couples after the disclosure of the unfavorable diagnosis; 2) analyze the bonds established with the fetuses, medical team and psychologist, as well as the bonds between both partners of each couple; 3) understand the parents desires related to the future and development of their pregnancies, and also describe the psychologist s perceptions of the analyzed cases. The analysis was accomplished through the clinical method, by using a technique of free, non-directive interviews. The Theory of Objective Relationships developed by Melanie Klein was adopted to interpret the contents, which was complemented with information provided by other psychological studies on gestations of malformed fetuses. The framework was adjusted to the institutional conditions where prenatal diagnosis was performed. Results reveal how difficult it is for couples to understand the medical information provided to them at the time when diagnosis is concluded. In this moment, anguish and blame were typical of the schyzo-paranoid position. The parents and especially the women s narcissism was deeply affected. Denial, projection, and splitting were common defenses. Characteristic experiences of the depressive position signalized for a greater integration of the ego and for a decrease in defensive mechanisms. Emotional reactions, expectations, and feelings presented by the husbands were different than those presented by the wives. The bonds established with the fetuses were suddenly broken and strong, ambivalent feeling were found. The couples adopted a dependency-oriented position in relation to all professionals in the team. An attitude of respect to the couple s feelings and values favored them in the process of consideration and expression of feelings. Their decisions regarding abortion triggered a moral dilemma that was respected by the medical team. Beliefs and values of each couple, as well as their own personal evaluation of the malformation s severety were more determinant for the decision on the gestational future than gestational ages and bonds established with the fetuses. Suggestions were made to professionals enrolled in the assistance of Fetal Medicine. / Este estudo relata a viv?ncia emocional de quatro casais ap?s a descoberta de anomalias severas no feto em gesta??o. Objetivou-se: investigar as rea??es emocionais, ou seja, fantasias, ang?stias e defesas neles desencadeadas ap?s receberem o diagn?stico pr?-natal desfavor?vel; analisar os v?nculos estabelecidos com o feto, a equipe m?dica, a psic?loga e o c?njuge; compreender os desejos dos pais com rela??o ao futuro e ? evolu??o da gravidez, bem como descrever as viv?ncias da psic?loga com rela??o aos casos pesquisados. A an?lise foi realizada atrav?s do m?todo cl?nico, utilizando a t?cnica da entrevista livre, n?o-diretiva. Para interpretar os conte?dos analisados adotou-se a teoria das rela??es objetais desenvolvida por Melanie Klein, complementada por subs?dios de outros estudos psicol?gicos sobre gravidez de fetos malformados. O enquadre foi adaptado ?s condi??es da institui??o onde os casais realizaram o estudo pr?-natal. Os resultados revelaram o qu?o dif?cil ? a compreens?o das informa??es m?dicas transmitidas por ocasi?o do diagn?stico. Nesse momento, a ang?stia e a culpa foram t?picas da posi??o esquizo-paran?ide. O narcisismo parental, especialmente o da mulher, foi fortemente abalado. A nega??o, a proje??o e a cis?o foram as defesas mais utilizadas. As viv?ncias depressivas, marcantes na posi??o depressiva, sinalizaram uma maior integra??o eg?ica e uma diminui??o dos mecanismos defensivos. Os maridos apresentaram rea??es emocionais, expectativas e sentimentos diferentes das viv?ncias femininas. O v?nculo estabelecido com o feto foi bruscamente interrompido e fortes sentimentos de ambival?ncia foram encontrados. Os casais assumiram uma posi??o de depend?ncia em rela??o a todos os profissionais da equipe. Uma atitude de respeito ?s cren?as e aos valores dos pacientes favoreceu-os no processo reflexivo e na express?o dos sentimentos. A decis?o dos casais em rela??o ? interrup??o da gravidez desencadeou um conflito moral respeitado pela equipe. As cren?as e os valores de cada casal, bem como a avalia??o pessoal da gravidade do problema foram mais determinantes para a decis?o acerca do futuro da gravidez do que o tempo gestacional e o v?nculo at? ent?o estabelecido com o feto. Sugest?es foram oferecidas aos profissionais envolvidos no atendimento em Medicina fetal.

Abstract not available

"March 2003" / Includes bibliographical references (leaves 237-297) / ix, 297 leaves : ill. (some col.), plates (col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, School of Agriculture and Wine, Discipline of Animal Science, 2005

Includes bibliographical references. / 1 v. (various foliations) : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Indicates that retarded fetal growth in sheep, associated with restricted supply of substrates to the fetus, is accompanied by reduced concentrations of insulin-like growth factor I in fetal blood and its decreased production in several major fetal tissues. / Thesis (Ph.D.)--University of Adelaide, Dept. of Obstetrics and Gynaecology, 1995

[Truncated abstract] In obstetric practice, pregnant women at risk of pre-term delivery between 24 and 34 weeks of gestation are administered synthetic glucocorticoids (betamethasone or dexamethasone) to induce fetal organ maturation. During this gestational period, the fetal kidney is undergoing a phase of rapid organogenesis with an increase in renal growth and active nephrogenesis occurring. The studies comprising this thesis examine the effects of prenatal betamethasone exposure on the fetal and adult ovine kidney. The central hypothesis of these studies was that exposure of the fetal kidney to betamethasone in late gestation would change renal structure and induce long-term alterations in the expression of glucocorticoid-sensitive genes and proteins. In the fetal studies, pregnant Merino ewes bearing single fetuses received single or repeated-weekly intra-muscular (i.m.) injections of betamethasone (0.5 mg/kg body weight) or saline commencing on day 104 of gestation (term is 150 days). Kidneys were collected from fetuses at 109, 116, 121 and 146 days of gestation (d). Using gold standard unbiased stereological techniques, the physical disector/fractionator method, total glomerular (nephron) number and glomerular volume were determined in 146 d fetal kidneys exposed to repeated maternal saline or betamethasone administration. In the adult study, kidneys were collected from 3.5-year-old sheep that had been exposed to ... In this thesis I have demonstrated that renal growth restriction as a result of betamethasone exposure is associated with a reduction in fetal nephron endowment. Although betamethasone does not appear to consistently alter nephron number or glomerular size, it may indirectly affect total nephron endowment through effects on renal growth. I have also provided evidence which suggests that lategestation betamethasone exposure in sheep does not program permanent alterations in the renal expression of genes or proteins involved in glucocorticoid hormone action or components of the renin-angiotensin system. Therefore, exposure of the fetal kidney to betamethasone during nephrogenesis may alter renal structure if kidney growth is perturbed; however, there are no persistent alterations in the expression of glucocorticoid-sensitive genes. These findings are consistent with the preservation of normal basal blood pressure in the adult sheep I studied and with the limited results from human studies of late-gestation maternal glucocorticoid administration.

Submitted by (edna.saturno@ufrpe.br) on 2016-07-20T13:38:22Z No. of bitstreams: 1 Debora Monteiro Navarro Marques de Oliveira.pdf: 1171425 bytes, checksum: 51b74fd42ae58771f24380698850c0b6 (MD5) / Made available in DSpace on 2016-07-20T13:38:22Z (GMT). No. of bitstreams: 1 Debora Monteiro Navarro Marques de Oliveira.pdf: 1171425 bytes, checksum: 51b74fd42ae58771f24380698850c0b6 (MD5) Previous issue date: 2013-02-19 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The Doppler ultrasound is a tool that enable the study of the hemodynamic aspects of the patients and is widely used in veterinary medicine for the obstetrical evaluation of the maternal-fetal circulations, thus allowing to characterize the blood flow of the fetus and its viability. However, few studies have been conducted to date, involving the evaluation of Doppler index in fetuses of dogs. Therefore, the objective of this study was to determine Doppler parameters of the main vessels of canine fetuses with bradycardia, in pre-parturition period.Were determined resistance (IR) and pulsatility (IP) indices of the umbilical artery (UA), aorta (AO) and the caudal vena cava (VCC), two fetuses of each pregnant bitch, at gestational age between 58 and 60 days. The fetuses were divided into groups: on suffering (n= 27) and without suffering (n= 15), based on bradycardia (< 220 bpm), evaluated by ultrasonography Mode M of the umbilical artery. For statistical analysis were used the Pearson correlation test and analysis of variance (ANOVA), followed by Student T test. In the groups with and without bradycardia was observed significant difference (p < 0,05) between the average of FC, as well as of the parameters IRAU, IPAU, IRVCC showed statistically significant differences. For IPVC was observed a trend (p ≤ 0.09) to be higher in fetuses presenting suffering. High, positive and significant correlations were obtained between IRAU x IPAU, IRAU x IRVCC, IRAU x IPVCC, IPAU x IRVCC, IPAU x IPVCC, IRAA x IPAA, IRVCC x IPVCC, and moderate and positive between IRAA x IPVCC e IPAA x IPVCC. A moderate negative correlation was observed between FC x IRVCC (p < 0.05). Based on this results, were established that values great than, 0,7 (IRAU), 1,6 (IPAU) and 0,7 (IRVCC) characterize suffering fetuses. The definition of these parameters brings important contribution in the routine of clinical obstetrics in relation to the diagnosis of fetal distress, favoring the decision regarding the need to interventions during pregnancy in bitches. / A ultrassonografia Doppler tem possibilitado o estudo sobre os aspectos hemodinâmicos dos pacientes, sendo bastante utilizada na medicina veterinária para a avaliação obstétrica das circulações materno-fetais, permitindo caracterizar a relação do fluxo sanguíneo do feto com a sua viabilidade. No entanto, a avaliação de índices Dopplerfluxométricos para fetos da espécie canina, ainda é pouco estudada. O objetivo deste estudo foi determinar parâmetros Dopplerfluxométricos dos principais vasos de fetos de cães, com bradicardia, no período pré-parto. Foram determinados os índices de resistividade (IR) e de pulsatilidade (IP) da artéria umbilical (AU), artéria aorta (AA) e veia cava caudal (VCC), sendo avaliado o máximo de dois fetos por cadela gestante, com idade gestacional entre de 58 e 60 dias. Os fetos foram divididos em dois grupos com (< 220 bpm) e sem bradicardia, que foi avaliada através da ultrassonografia em Modo M, utilizando as artérias umbilical e aorta, e veia cava caudal. Para a análise estatística foram utilizados teste de correlação de Pearson e análise de variância (ANOVA), seguido de teste T de Student. Nos grupos com e sem bradicardia foi observada diferença significativa (p < 0,05) entre as médias de FC, assim como para os parâmetros IRAU, IPAU, IRVCC. Para o IPVC foi observada uma tendência (p ≤ 0,09) a ser maior nos fetos que apresentavam bradicardia. Obtiveram-se correlações altas, positivas e significativas entre IRAU x IPAU, IRAU x IRVCC, IRAU x IPVCC, IPAU x IRVCC, IPAU x IPVCC, IRAA x IPAA, IRVCC x IPVCC, e moderadas e positivas entre IRAA x IPVCC e IPAA x IPVCC. Uma correlação moderada e negativa foi observada entre FC x IRVCC (p < 0,05). Com base nesses resultados, pode-se estabelecer que o sofrimento fetal, ocorre quando os valores forem maiores que: 0,7 para IRAU, 1,6 para IPAU e 0,7 para IRVCC. A definição destes parâmetros traz importante contribuição na rotina clínico-obstétrica referente ao diagnóstico de sofrimento fetal, favorecendo a tomada de decisões quanto a necessidade de intervenções durante o período gestacional de cadelas.

O pelo é uma característica dos mamíferos: na maioria das espécies, juntamente com a pele, a pelagem reveste externamente o corpo, com exceção de algumas regiões bem delimitadas. O pelo do cão e demais mamíferos apresenta formato distinto de acordo com a região do corpo. O estudo da obtenção e caracterização de células-tronco em cães poderá ampliar as possibilidades terapêuticas, mediante a utilização das células indiferenciadas na reparação capilar e lesões da pele e seus anexos. Neste trabalho foram utilizadas amostras de folículo piloso retiradas da região rostral de fetos caninos correspondentes a três fases gestacionais distintas (grupo I= 30 dias de gestação; grupo II= 35 dias de gestação; grupo III= 40 dias de gestação).Estas amostras foram cultivadas em placas de Petri de 75 cm² com 5mL de meio de cultivo DMEM, suplementado com 10% de soro fetal bovino (SFB) e 1% de antibióticos estreptomicina/penicilina. As células obtidas foram congeladas e descongeladas e utilizados nos procedimentos de, análise da expressão dos marcadores de células-tronco: STRO-1, CD 117, OCT 3/4, CD 90, Nanog, CD- 34, SSEA-4, CD -105, MCP-1, HSP- 47, CD 1 A, VEGFR1, DR4, IL- 1 &beta;, caspase3, Ki- 67, CD -45. A escolha dos anticorpos marcadores de células-tronco foi baseada em estudos que mostraram afinidade e avidez com o folículo piloso. Utilizamos também para a caracterização do folículo piloso, a imunohistoquímica e análise de PCR com o marcador S100 e a imunocitoquímica com os marcadores OCT 3/4, VEGF, STRO-1, CD117 e Nanog. Os resultados obtidos sugerem que a linhagem celular do folículo piloso de fetos de cães apresentaram crescimento satisfatório com a utilização do meio DMEM-hight suplementado com 10% de SFB inativado e 1% de antibióticos, sendo mantidas e expandidas em cultivo. O crescimento e a expansão das células-tronco do folículo piloso são peculiares, pois, sua expansão ocorre em torno da haste pilosa, utilizando-a como ancoragem. Os resultados indicaram características de pluripotência nas células-tronco do folículo piloso pela expressão dos marcadores OCT3/4, Nanog, CD105, CD90, SSEA-4, e STRO-1, em todos os grupos analisados. Considerando, a taxa de proliferação, a fase do ciclo celular, distribuição e aspectos de morte celular não foram encontradas diferenças significantes na expressão dos marcadores HSP47, DR4. Para verificar a angiogêneseforam utilizados os marcadores VEGFR1, Ki-67 e caspase-3, sendo expressos em todos os grupos. Na imunohistoquímica e análise de PCR observou-se a expressão da proteína S100, sendo maior nas células do grupo de fetos com 40 dias de gestação- grupo III. Neste estudo, podemos concluir que o conjunto de marcadores expressos nos diferentes grupos de células tronco obtidas das vibrissas de fetos caninos indicaram a expressão de marcadores de pluripotência das células-tronco do folículo piloso / Hair is a characteristic of mammals, since in most species the coat spreads throughout the body, except for some well-defined regions. Mammals in general, including dogs, have different characteristics in their coat according to the region of the body. The study of obtainment and characterization of stem cells from the hair follicle could contribute to new therapeutic possibilities, particularly in the treatment of hair, skin and it appendices injuries. In this study, hair follicle samples were harvestedin the rostral region of canine fetuses, divided into three distinct groups, according to the stage of pregnancy, as follows: Group I = 30 days, Group II = 35 days, Group III = 40 days. These samples were grown in 75 cm² Petri dishes with 5 mL of DMEM culture medium supplemented with 10% fetal bovine serum (FBS) and antibiotics 1% streptomycin / penicillin. Cells were frozen and thawed and used in procedures for analysis of expression of markers of stem cells: STRO-1, CD 117, OCT 3/4, CD 90, Nanog, CD- 34,SSEA -4,CD- 105, MCP-1, HSP-47, CD 1 A, VEGF-R1, DR4, IL-1 &beta;, caspase-3, Ki-67, CD -45. The choice of antibodies was based on studies that showed affinity and avidity to the hair follicle. In addition, for purposes of characterization of the hair follicle, procedures for immunohistochemistry and PCR analysis with the marker S100 and immunocytochemistry with the markers OCT3/4, VEGF, STRO-1, CD117 e Nanog were used. The results suggested that hair follicle cell line of fetal dogs showed satisfactory growth using the DMEM high medium supplemented with 10% inactivated FBS and 1% antibiotics and could be maintained and expanded in culture. The growth and expansion of stem cells of the hair follicle are unique because they occur around the hair shaft using it as anchorage. Results also indicated pluripotency features in the hair follicle stem cells through the positive expression of Oct3/4, Nanog, CD105, CD90, SSEA-4 and STRO-1, in all groups. Regarding the proliferation rate and cell cycle phase, distribution and cell death aspects, HSP47, DR4 were used. To verify angiogenesis, VEGFR1, Ki67 and Caspase-3 were expressed in all groups. In Immunohistochemistry procedures we observed the expression of S100, and in mRNA on RT-PCR. We concluded in this study that in group III the expression of S100 was higher than in the two other groups. In addition, we found that the pluripotency of the stem cells was indicated by the expression of the markers OCT3/4, Nanog, CD105, CD90, SSEA-4 and STRO-1