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Thesis (PhD (Veterinary Tropical Diseases)--University of Pretoria, 2009. / Includes bibliographical references. Also available in print format.

Thesis (Ph.D.) - University of Glasgow, 2008. / Ph.D. thesis submitted to the Faculty of Biomedical and Life Sciences, Division of Immunology, Infection & Inflammation, University of Glasgow, 2007. Includes bibliographical references. Print version also available.

Made available in DSpace on 2016-06-21T13:43:13Z (GMT). No. of bitstreams: 2 178.pdf: 1437974 bytes, checksum: 047540ba121ca5948b540d6c7496106b (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2014 / Made available in DSpace on 2016-07-05T22:17:02Z (GMT). No. of bitstreams: 3 178.pdf.txt: 94268 bytes, checksum: ed8e1d8b0ae23b6bb21a951068e65500 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) 178.pdf: 1437974 bytes, checksum: 047540ba121ca5948b540d6c7496106b (MD5) Previous issue date: 2014 / Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil / Este trabalho analisou o efeito do tratamento em massa com doses únicas anuais de Dietilcarbamazina (DEC), no período de 2007 a 2012, em indivíduos infectados por Wuchereria bancrofti, residentes em Olinda - PE. Para essa análise foram utilizadas as técnicas de filtração em membrana, na detecção da microfilaremia, o teste do cartão ICT e o Og4C3-ELISA, na detecção do antígeno circulante filarial, e o teste BM14 na avaliação dos níveis de anticorpos antifilariais. Os resultados obtidos indicam redução nas características avaliadas: após a quarta dose de DEC, a microfilaremia reduziu 100 por cento e a antigenemia pelo cartão ICT atingiu 78,1 por cento de redução após a quinta dose. A mediana do Og4C3 caiu significativamente de 7117 ua, para 1715 ua após a terceira dose, último ano que o teste foi realizado. Observou-se curva de redução também nos níveis de Bm14, com mediana da densidade ótica caindo de 2,1 para 0,1 após a quinta dose. A diminuição nas taxas das características estudadas indica que o tempo preconizado pela Organização Mundial de Saúde para a eliminação da transmissão da FL na área é suficiente para a negativação das microfilárias. Os resultados desse estudo mostram a elevada eficácia do esquema terapêutico utilizado no clareamento da microfilaremia e tratamento dos infectados, e sugerem que a utilização desse esquema na população possivelmente tenha levado a interrupção da transmissão na área. Sugere-se que haja um acompanhamento maior que cinco anos da população submetida ao tratamento para uma melhor avaliação dos níveis de anticorpos e de antigenemia filarial circulante

Based on two surveys, the thesis focuses on the prevalence of filarial parasites of domestic carnivores in Gauteng, KwaZulu-Natal and Mpumalanga provinces in South Africa and Maputo province of Mozambique. This is complemented by diagnostic results of routine examinations for filarial infections of dogs and cats from South Africa obtained between 1994 and 2008. Blood samples were collected and initially screened by membrane filtration for microfilariae. Other techniques employed were acid phosphatase staining for the identification of microfilariae and a commercial enzymelinked immunosorbent assay for the detection of heartworm antigen. Combined with a critical literature review on filariosis of domestic carnivores in Africa, which is updated by diagnostic results obtained from animals in Africa between 1992 and 2008, the topic is addressed for the first time ever from a continental perspective. In the South African provinces and Maputo province of Mozambique 196 of 1 379 dogs (14.21 %) were found positive for microfilariae. The species identified were Dirofilaria immitis, Dirofilaria repens, Acanthocheilonema reconditum and Acanthocheilonema dracunculoides. The endemic status of D. immitis was confirmed in 2 out of 313 dogs from Maputo province but not in the South African provinces. Infection with D. repens was found in 70 dogs (5.08 %). The highest prevalence rate was recorded in KwaZulu-Natal with 12.47 % (52/417), followed by Maputo Province with 3.83 % (12/313) and Mpumalanga with 1.5 % (5/333). Routine examinations have also confirmed autochthonous infections with D. repens in Gauteng and North West provinces. Acanthocheilonema reconditum was the species with the highest overall prevalence of 8.85 % (122/1 379). The highest prevalence rate was recorded in Mpumalanga with 29.13 % (97/333) followed by Maputo province with 6.39 % (20/313) and KwaZulu-Natal with 1.2 % (5/417). Routine examinations have also confirmed autochthonous infections in Gauteng, North West and Western Cape provinces. Acanthocheilonema dracunculoides was the species with the lowest overall prevalence of 0.07 % (1/1 379) and was only recorded in 1 dog from Maputo Province. In KwaZulu-Natal 9 of 82 cats (10.98 %) were found positive for microfilariae, with D. repens as the only species involved. / Thesis (PhD)--University of Pretoria, 2009. / Veterinary Tropical Diseases / unrestricted

Brugia malayi is a filarial nematode causing lymphatic filariasis in humans characterized by swelling of the lower extremities. The aim of this study was to conduct a real time PCR (qRT-PCR) to verify gene expression levels of Brugia malayi nematodes treated with 20 hydroxyecdysone. Transcriptome analysis was previously performed resulting in the identification of 44 genes that were upregulated by exposure to 20-hydroxyecdysone. Based on transcriptome results, known GO Terms and functions, four genes and one endogenous housekeeping gene were chosen for validation by RT-PCR. Induced samples showed a mean increase of microfilarie by 2.2 fold. Induced wells exhibited a 2.8 fold increase of pre- microfilarie production. On day two adult females treated with 20-HE displayed 3.8-fold increase of microfilaria production as compared to uninduced controls. Overall, all four genes showed upregulation with treatment of 20-hydroxyecdysone at levels that corresponded to the results obtained from the transcriptome analysis. Findings in this experiment expand on the understanding of the ecdysone response system in Brugia malayi, which could serve as a potential drug target against filarial disease.

Thesis (Ph.D.)--Tufts University, 2001. / Adviser: Juliet A. Fuhrman. Submitted to the Dept. of Biology. Includes bibliographical references (leaves 42-54). Access restricted to members of the Tufts University community. Also available via the World Wide Web;

Dissertation (Ph.D.)--University of Toledo, 2007. / Typescript. "Submitted as partial fulfillment of the requirements for The Doctor of Philosophy iin Biology." Bibliography: leaves 96-105.

Thesis (M.Sc.). / Written for the Institute of Parasitology. Title from title page of PDF (viewed 2009/08/07). Includes bibliographical references.