In Uganda, there are an estimated 200,000 women suffering from obstetric fistula, with 1,900 new cases expected annually. These figures, combined with a persistently high maternal mortality rate, have led to an international discourse that claims the solution to improving maternal health outcomes is facility-based delivery with a skilled birth attendant. In accord with this discourse, the Ugandan government criminalized traditional birth attendants in 2010. In this study, I examine the lived experience of traditional birth attendants and women who have suffered from an obstetric fistula in eastern Uganda. Using data collected from open-ended, semi-structured interviews, focus groups, and participant-observation, I describe the biocultural determinants of obstetric fistula. Based on findings, I argue that although emergency obstetric care is critical to prevent obstetric fistula in cases of obstructed labor, the criminalization of the locally constructed system of care, TBAs, serves as yet another layer of structural violence in the lives of rural, poor women. Results demonstrate how political-economic and cultural determinants of obstetric fistula are minimized in favor of a Western prescribed, bio-medical solution, which is heavily resource dependent. This solution is promoted through a political economy of hope fueled by the obstetric imaginary, or the enthusiastic belief in Western-style biomedical obstetric care’s ability to deliver positive health outcomes for women and infants regardless of local context and constraints. Recommendations include increased obstetric fistula treatment facilities with improved communication from medical staff, decriminalization of traditional birth attendants and renewed training programs, and engaging local populations in maternal health discourse to ensure culturally competent programs. / Graduation date: 2013

As fístulas broncopleurais habitualmente decorrem de procedimentos cirúrgicos de ressecção pulmonar por diversas etiologias, com incidência na literatura de 0% a 28%, e mortalidade de 16% a 72%, sendo mais frequente em homens, e nos casos de pneumonectomia do que em lobectomia. As deiscências totais de coto brônquico apresentam indicação de tratamento cirúrgico, porém a condição clínica destes pacientes geralmente é precária com alto risco anestésico e cirúrgico. Os tratamentos endoscópicos de fístulas broncopleurais até então descritos foram utilizados apenas em fístulas parciais menores que 8 milímetros. Objetivo: Este estudo propõe-se a avaliar a viabilidade do tratamento endoscópico de fístulas totais de coto brônquico utilizando o dispositivo oclusor de defeitos septais cardíacos Occlutech-Fígulla®. Casuística e métodos: Foram incluídos pacientes com fistula broncopleural total secundária a ressecção pulmonar. Os pacientes foram submetidos inicialmente à broncoscopia para avaliação e medida da fístula e a uma cintilografia pulmonar de inalação para documentação do padrão inicial de vazamento. A colocação do dispositivo foi feita através da broncoscopia realizada sob sedo-analgesia com o paciente em ventilação espontânea com suplementação de oxigênio. Os pacientes foram acompanhados durante 12 meses e avaliados quanto à cobertura do dispositivo por tecido cicatricial, fechamento do trajeto fistuloso e desenvolvimento de complicações relacionadas como o deslocamento do dispositivo, lesões de estruturas adjacentes e desenvolvimento de infecção. As análises descritivas dos dados quantitativos com distribuição normal foram apresentadas através das médias acompanhadas dos respectivos desvios padrão. Os dados sem distribuição normal foram apresentados através de suas medianas com os respectivos intervalos interquartil 25-75%. A análise inferencial utilizou a Análise de Variância de Medidas Repetidas para os dados com distribuição normal e os testes não-paramétricos Anova de Friedman para os dados que não apresentavam distribuição normal. Foi considerada uma probabilidade de erro do tipo I (alfa) de 0,05. Resultados: Foram selecionados nove pacientes com predomínio do sexo masculino (77,8%), com média de idade de 45 ±11,1 anos, com ressecções motivadas em sua maioria por sequelas de doença infecciosa (78%), com predomínio de pneumonectomia direita (66,6%), com fístulas que apresentavam diâmetro de 6 a 17 mm. Do grupo de 9 pacientes tratados tivemos três casos de fechamento completo, dois casos de fechamento parcial, duas falhas sendo uma por deslocamento e retirada do dispositivo e outra por retirada ao término do período de seguimento com permanência dos sintomas e dois óbitos não relacionados. Durante o período de acompanhamento não evidenciamos complicações infecciosas ou lesão de estruturas adjacentes relacionadas à permanência do dispositivo. Conclusão: O dispositivo para tratamento de defeitos do septo atrial pode ser uma alternativa no tratamento endoscópico de fístulas totais de coto brônquico, funcionando como tratamento definitivo em alguns casos e servindo como suporte nos pacientes que aguardam melhora das condições clínicas para uma intervenção cirúrgica tardia com menor risco. Não foram detectados eventos graves como infecções ou lesão vascular relacionados à presença do dispositivo / Bronchopleural fistulas are possible complications following lung resection procedures for different etiologies. The reported incidence is 0 % to 28%, and the related mortality is 16% to 72%. More frequently in men and pneumonectomy cases than lobectomy cases. Total dehiscence of the bronchial stump should be treated by surgical interventions; however, the clinical status of these patients is generally poor with high anesthetic and surgical risks. Endoscopic treatment of bronchopleural fistulas previously described were used only in 8mm or smaller partial fistulas. Objective To evaluate the endoscopic treatment of total bronchial stump fistulas using the Occlutech - Fígulla®, a device used to close cardiac septal defects. Patients and methods: We select patients with total bronchial stump fistula. Patients underwent bronchoscopy for local fistula evaluation and an inhalation lung scintigraphy for the initial leak parameter documentation. The placement of the device was made by bronchoscopy performed under sedation - analgesia with the patient in spontaneous ventilation with oxygen supplementation. Patients were followed for 12 months and assessed for scar tissue coverage device, fistula closure and development of related complications such as displacement device, adjacent structures lesions and infection. The descriptive analysis of quantitative data with normal distribution were presented through the mean along with the related standard deviations. Non-normal distribution data were presented by their medians with their respective interquartile ranges 25-75 %. The inferential analysis used Repeated Measures Analysis of Variance for data with normal distribution and non-parametric tests of Friedman ANOVA to data with nonnormal distribution. It was considered an error probability of a type I (alfa) 0.05. Results: This study evaluated nine patients with a males predominance (77.8% ) with mean age of 45 ± 11.1 years with resections for sequelae of infectious disease (78%), predominantly right pneumonectomy (66.6% ), with fistulas diameter ranging from 6 to 17 mm. The group of 9 patients had three cases of complete closure, two cases of partial closure, two failures one per displacement and removal of the device another for withdrawal at the end of follow-up with persistence of symptoms and two unrelated death. During the follow-up period was not detect complications such as infections or injury to adjacent structures related to the device. Conclusion: The device for treatment of atrial septal defects can be an alternative to the endoscopic treatment of total fistula bronchial stump, functioning as definitive treatment in some cases and serving as a support for patients awaiting improvement of clinical conditions for a later surgical intervention with lower risk. No severe events were detected as infections or vascular injury related to the device

Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2018-04-16T13:26:11Z No. of bitstreams: 2 Dissertação - Jéssica Guimarães Rodrigues - 2018.pdf: 4231969 bytes, checksum: 7cd99fc018c0461eb571ee4a66eb3bbc (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-04-16T13:27:10Z (GMT) No. of bitstreams: 2 Dissertação - Jéssica Guimarães Rodrigues - 2018.pdf: 4231969 bytes, checksum: 7cd99fc018c0461eb571ee4a66eb3bbc (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-04-16T13:27:10Z (GMT). No. of bitstreams: 2 Dissertação - Jéssica Guimarães Rodrigues - 2018.pdf: 4231969 bytes, checksum: 7cd99fc018c0461eb571ee4a66eb3bbc (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-03-16 / Hemodialysis is the most common category of kidney replacement therapy set for chronical kidney disease. In order to perform this treatment it’s needed a vascular access (VA) that offers an adequate flow rate, a long use-life and a low rate of complications. The Arteriovenous Fistula (AVF) is the closest access to meet these requirements. It can, however, present complications and, during the cannulation that usually happens three times per week, adverse events (AE) can occur to the patient. The arteriovenous fistula cannulation must happen with safety in order to prevent future patency problems. There are three methods of cannulation: area, rope ladder and buttonhole. In the area method, the insertion points of the needles are in the same area; in the rope ladder method there’s the varying of the place of the puncture, at a distance defined by the previous puncture, all along the VA; and in the buttonhole method, the needle’s insertion happen in the same place, angle and deepness, forming a subcutaneous tunnel that will be cannulated with the blunt needle. Each one of these methods has its own particularity and can influence in the need to repair the fistula. This is a prospective cohort study, during the course of six months, from April to September of 2017, conducted with the participation of 347 patients using the vascular access by autologous arteriovenous fistula, within three hemodialysis clinics in the city of Goiânia - GO. The data collection happened by weekly interview to the patients, using a structured instrument online. The research was approved by the Ethics Committee and the participation conditioned to signing of the consent form by the patient. The general objective was to analyze the factors that can influence in the necessity to repair the arteriovenous fistula of patients in hemodialytic treatment cannulated by different cannulation methods. The specific objective was to relate the adverse events and complications in the different arteriovenous fistula cannulation methods. We’ve found that in the buttonhole method, the most frequent AE was dermatitis and misscannulation, and in the area/rope-ladder methods, the most frequent AE were haematoma and peri-punction bleeding. The patients in the buttonhole method group received the hemodialytic treatment with a higher blood flow compared the other group. We’ve observed that the dual lumen catheter (DLC) is a predictor to the need of AVF repairments, due to enhancing in 28% the risk of need for AFV repair. The “arterial” retrograde cannulation has presented itself as a protection factor, diminishing the need to AVF repairments in 1%. In conclusion, the buttonhole method is recommended, since there is an intermittent surveillance of the arteriovenous fistula by the nurse in the touching exam. The area method is not recommended, and the rope ladder method should be individually evaluated in future studies. The nurse must act by monitoring the AFV, surveillance of the patency parameters and health education to the patients for the AVF self-care, as well as continued education to the nursing team in order to promote safe and scientifically based practices. / A hemodiálise é a modalidade de terapia renal substitutiva mais comumente instituída para a doença renal crônica. Para esse tratamento é necessário um acesso vascular que ofereça fluxo sanguíneo adequado à necessidade dialítica, meia vida longa e baixo índice de complicações. A fístula arteriovenosa é o acesso que mais se aproxima desses requisitos. Porém, não obstante, pode apresentar complicações, e durante as punções, que comumente se repetem três vezes por semana, pode haver eventos adversos (EA) ao paciente. A punção da fístula arteriovenosa deve ser realizada com segurança a fim de prevenir futuros problemas de perviedade. Há três métodos de punção: regional, escada de corda, e buttonhole. No método regional, os pontos de inserção das agulhas são na mesma região; no método escada de corda, há rotação do sítio de punção, a uma distância definida a partir da anterior ao longo de todo o AV; e no buttonhole, a inserção da agulha é no mesmo local, ângulo e profundidade, formando de um túnel subcutâneo que será puncionado com agulha romba. Cada um desses métodos tem sua particularidade e podem influenciar na necessidade para reparos na fístula. Este é um estudo longitudinal de coorte prospectiva, no período de seis meses, abril a setembro de 2017, realizado com 347 pacientes em hemodiálise usando acesso vascular por fístula arteriovenosa autóloga, em três clínicas satélites do município de Goiânia - GO. A coleta de dados foi por entrevista semanal aos pacientes, por meio de instrumento estruturado online. A pesquisa foi aprovada por comitê de ética, e a participação condicionada à assinatura do Termo de Consentimento Livre e Esclarecido do paciente. O objetivo geral foi analisar fatores que influenciam na necessidade de reparo à fístula arteriovenosa de pacientes em hemodiálise puncionados por distintos métodos de punção. E os objetivos específicos foram identificar e relacionar os eventos adversos e complicações em distintos métodos de punção da fístula arteriovenosa, e caracterizar os preditores de complicações da fístula arteriovenosa. Encontramos como resultados que no método de punção de fístula arteriovenosa buttonhole o EA mais frequente foi dermatite e reinserção de agulhas de punção, e nos métodos escada/regional foram hematoma e sangramento peripunção. Os pacientes no grupo puncionado pelo método buttonhole receberam hemodiálise sob fluxos de sangue mais altos comparado ao outro grupo. Observamos que o uso do cateter venoso central de duplo lúmen (CDL) caracteriza-se um preditor de necessidade de reparo da fístula arteriovenosa, pois aumenta em 28% o risco dessa necessidade. A punção “arterial” retrógrada apresentou-se como fator de proteção, diminuindo em 1% a necessidade de reparos. Concluímos que o método de punção buttonhole é recomendado desde que haja a monitoração intermitente da fístula arteriovenosa pelo enfermeiro durante exame físico. O método regional é desestimulado. E o método escada de corda deve ser avaliado individualmente em estudos futuros. O enfermeiro deve estabelecer a vigilância dos parâmetros de perviedade, educação em saúde para autocuidado da fístula arteriovenosa, bem como educação continuada para a equipe de enfermagem a fim de promover práticas seguras e cientificamente embasadas.

<p>Patients with idiopathic anal fistula (n=85) were compared with 215 control subjects, matched for age and sex, through a 180-item questionnaire. Obesity, smoking, constipation and bowel symptoms associated with IBS were more common in the patients.</p><p>Endoanal ultrasound (EUS) and magnetic resonance imaging (MRI) were compared in the preoperative evaluation of anal fistula in 23 patients. For classifying the primary tract, EUS and surgical findings agreed in 14 cases, and MRI and surgery for 11: for identifying an internal opening, the corresponding figures were 17 and 10.</p><p>Healing and sphincter function were studied in 42 patients operated with fistula excision and closure of the internal opening. Twenty-three patients healed primarily and another 10 after one re-operation, whereas nine required further surgery until healed. Anal resting pressure was reduced after three and 12 months, and squeeze pressure after 12 months.</p><p>Eighty-three patients were randomised to surgery with or without application of gentamicin-collagen underneath the flap: 26/42 of patients randomised to gentamicin-collagen healed primarily compared with 21/41 of patients randomised to surgery only (n.s).</p><p>Micro perfusion in the flap was studied by laser Doppler flowmetry during surgery in 16 patients. No correlation was seen between change in blood flow during surgery and non-healing/recurrence of the fistula.</p><p>In conclusion, obesity, functional bowel symptoms and possibly smoking are more common in patients with idiopathic anal fistula than in the general population. Endoanal ultrasound is a useful tool in the preoperative evaluation of anal fistula. Advancement flap repair has a reasonably high primary recurrence rate and healing is not significantly improved by local application of gentamicin-collagen: impaired intraoperative blood perfusion of the flap is an unlikely reason for non-healing. A decrease in continence occurs also after this kind of surgery, probably due to an impaired internal anal sphincter function.</p>

Patients with idiopathic anal fistula (n=85) were compared with 215 control subjects, matched for age and sex, through a 180-item questionnaire. Obesity, smoking, constipation and bowel symptoms associated with IBS were more common in the patients. Endoanal ultrasound (EUS) and magnetic resonance imaging (MRI) were compared in the preoperative evaluation of anal fistula in 23 patients. For classifying the primary tract, EUS and surgical findings agreed in 14 cases, and MRI and surgery for 11: for identifying an internal opening, the corresponding figures were 17 and 10. Healing and sphincter function were studied in 42 patients operated with fistula excision and closure of the internal opening. Twenty-three patients healed primarily and another 10 after one re-operation, whereas nine required further surgery until healed. Anal resting pressure was reduced after three and 12 months, and squeeze pressure after 12 months. Eighty-three patients were randomised to surgery with or without application of gentamicin-collagen underneath the flap: 26/42 of patients randomised to gentamicin-collagen healed primarily compared with 21/41 of patients randomised to surgery only (n.s). Micro perfusion in the flap was studied by laser Doppler flowmetry during surgery in 16 patients. No correlation was seen between change in blood flow during surgery and non-healing/recurrence of the fistula. In conclusion, obesity, functional bowel symptoms and possibly smoking are more common in patients with idiopathic anal fistula than in the general population. Endoanal ultrasound is a useful tool in the preoperative evaluation of anal fistula. Advancement flap repair has a reasonably high primary recurrence rate and healing is not significantly improved by local application of gentamicin-collagen: impaired intraoperative blood perfusion of the flap is an unlikely reason for non-healing. A decrease in continence occurs also after this kind of surgery, probably due to an impaired internal anal sphincter function.

The issue of chronic renal failure and cannulation of permanent venous accesses in patients treated at haemodialysis centres is a topical and widespread problem of these days. According to generally accessible resources the incidence of terminal stage of renal failure, and thus the necessity to treat a patient by means of elimination methods, is very high, it reaches four individuals per thousand inhabitants according to the statistics. It is obviously a widespread nursing problem. The theoretical part of the thesis is divided into a summary and description of the present situation in chronic renal failure, particularly a brief insight into the chronic renal failure and its causes and syndromes. It also deals with the topic of the present treatment possibilities with stress on patient treatment at nephrologic outpatient clinics and in haemodialysis centres. The next chapter of the thesis describes the development from haemodialysis history up to the present advanced elimination methods. The present elimination methods bring the necessity of functional provision of venous access, particularly cannulation of AV fistulas, it is a very frequent nursing operation provided by nurses treating chronically affected patients at haemodialysis centres. This chapter is concluded by general knowledge of the techniques of cannulation of permanent venous accesses, possible occurrence of complications during the cannula penetration itself in relation to the physical phase of nursing treatment about patients with chronic renal failure. The practical part of the thesis was aimed at discovering or possible confirmation of the hypotheses discussed in the theoretical part. The practical research was based on searching for problems among dialyzed patients related to the permanent venous access, on finding the approach of nurses working at dialysis centres to cannulation of permanent accesses and on mapping the access of patients with permanent venous access to nurses with short time experience at a dialysis centre. Research questions were determined for this purpose and were processed within qualitative research into case reports upon depth interviews with nurses working at the dialysis centres of České Budějovice Hospital, Český Krumlov Hospital and Písek Hospital and their patients. The research data were processed into charts, where responses from patients and those from nurses were assessed separately. Occurrence frequency of the individual answers was particularly monitored in the individual output tables, however the responses were not processed by means of standard statistic methods because of the chosen methodology and thus a low number of respondents, but the output data served for drawing conclusions and preparing recommendations for application of nursing methods. The thesis conclusion summarizes the obtained knowledge and recommends possible procedures of solving the problems of cannulation of permanent venous accesses in patients with chronic renal failure treated at haemodialysis centres.

As fístulas broncopleurais habitualmente decorrem de procedimentos cirúrgicos de ressecção pulmonar por diversas etiologias, com incidência na literatura de 0% a 28%, e mortalidade de 16% a 72%, sendo mais frequente em homens, e nos casos de pneumonectomia do que em lobectomia. As deiscências totais de coto brônquico apresentam indicação de tratamento cirúrgico, porém a condição clínica destes pacientes geralmente é precária com alto risco anestésico e cirúrgico. Os tratamentos endoscópicos de fístulas broncopleurais até então descritos foram utilizados apenas em fístulas parciais menores que 8 milímetros. Objetivo: Este estudo propõe-se a avaliar a viabilidade do tratamento endoscópico de fístulas totais de coto brônquico utilizando o dispositivo oclusor de defeitos septais cardíacos Occlutech-Fígulla®. Casuística e métodos: Foram incluídos pacientes com fistula broncopleural total secundária a ressecção pulmonar. Os pacientes foram submetidos inicialmente à broncoscopia para avaliação e medida da fístula e a uma cintilografia pulmonar de inalação para documentação do padrão inicial de vazamento. A colocação do dispositivo foi feita através da broncoscopia realizada sob sedo-analgesia com o paciente em ventilação espontânea com suplementação de oxigênio. Os pacientes foram acompanhados durante 12 meses e avaliados quanto à cobertura do dispositivo por tecido cicatricial, fechamento do trajeto fistuloso e desenvolvimento de complicações relacionadas como o deslocamento do dispositivo, lesões de estruturas adjacentes e desenvolvimento de infecção. As análises descritivas dos dados quantitativos com distribuição normal foram apresentadas através das médias acompanhadas dos respectivos desvios padrão. Os dados sem distribuição normal foram apresentados através de suas medianas com os respectivos intervalos interquartil 25-75%. A análise inferencial utilizou a Análise de Variância de Medidas Repetidas para os dados com distribuição normal e os testes não-paramétricos Anova de Friedman para os dados que não apresentavam distribuição normal. Foi considerada uma probabilidade de erro do tipo I (alfa) de 0,05. Resultados: Foram selecionados nove pacientes com predomínio do sexo masculino (77,8%), com média de idade de 45 ±11,1 anos, com ressecções motivadas em sua maioria por sequelas de doença infecciosa (78%), com predomínio de pneumonectomia direita (66,6%), com fístulas que apresentavam diâmetro de 6 a 17 mm. Do grupo de 9 pacientes tratados tivemos três casos de fechamento completo, dois casos de fechamento parcial, duas falhas sendo uma por deslocamento e retirada do dispositivo e outra por retirada ao término do período de seguimento com permanência dos sintomas e dois óbitos não relacionados. Durante o período de acompanhamento não evidenciamos complicações infecciosas ou lesão de estruturas adjacentes relacionadas à permanência do dispositivo. Conclusão: O dispositivo para tratamento de defeitos do septo atrial pode ser uma alternativa no tratamento endoscópico de fístulas totais de coto brônquico, funcionando como tratamento definitivo em alguns casos e servindo como suporte nos pacientes que aguardam melhora das condições clínicas para uma intervenção cirúrgica tardia com menor risco. Não foram detectados eventos graves como infecções ou lesão vascular relacionados à presença do dispositivo / Bronchopleural fistulas are possible complications following lung resection procedures for different etiologies. The reported incidence is 0 % to 28%, and the related mortality is 16% to 72%. More frequently in men and pneumonectomy cases than lobectomy cases. Total dehiscence of the bronchial stump should be treated by surgical interventions; however, the clinical status of these patients is generally poor with high anesthetic and surgical risks. Endoscopic treatment of bronchopleural fistulas previously described were used only in 8mm or smaller partial fistulas. Objective To evaluate the endoscopic treatment of total bronchial stump fistulas using the Occlutech - Fígulla®, a device used to close cardiac septal defects. Patients and methods: We select patients with total bronchial stump fistula. Patients underwent bronchoscopy for local fistula evaluation and an inhalation lung scintigraphy for the initial leak parameter documentation. The placement of the device was made by bronchoscopy performed under sedation - analgesia with the patient in spontaneous ventilation with oxygen supplementation. Patients were followed for 12 months and assessed for scar tissue coverage device, fistula closure and development of related complications such as displacement device, adjacent structures lesions and infection. The descriptive analysis of quantitative data with normal distribution were presented through the mean along with the related standard deviations. Non-normal distribution data were presented by their medians with their respective interquartile ranges 25-75 %. The inferential analysis used Repeated Measures Analysis of Variance for data with normal distribution and non-parametric tests of Friedman ANOVA to data with nonnormal distribution. It was considered an error probability of a type I (alfa) 0.05. Results: This study evaluated nine patients with a males predominance (77.8% ) with mean age of 45 ± 11.1 years with resections for sequelae of infectious disease (78%), predominantly right pneumonectomy (66.6% ), with fistulas diameter ranging from 6 to 17 mm. The group of 9 patients had three cases of complete closure, two cases of partial closure, two failures one per displacement and removal of the device another for withdrawal at the end of follow-up with persistence of symptoms and two unrelated death. During the follow-up period was not detect complications such as infections or injury to adjacent structures related to the device. Conclusion: The device for treatment of atrial septal defects can be an alternative to the endoscopic treatment of total fistula bronchial stump, functioning as definitive treatment in some cases and serving as a support for patients awaiting improvement of clinical conditions for a later surgical intervention with lower risk. No severe events were detected as infections or vascular injury related to the device

Applying chemical coagulants and auxiliary coagulants in wastewater treatment has become more popular in Vietnam. Although the efficacy of chemical coagulants has been well recognized, there are disadvantages associated with the use of these products, such as the inefficiency at low temperatures, increasing the residual cation in solution, causing health problems and distribution water, relatively high cost, producing high volume of sludge. Thus, it is desirable to replace these chemical coagulants for products that do not generate such drawbacks, such as natural polymers. In this paper, the authors conducted experiments by using natural auxiliary coagulants extracted from seeds of Cassia fistula (gum MHY) and chemical polymer as auxiliary coagulation to treat textile wastewater with basic polluted parameters: pH = 9.0; COD = 800 mgO2/L, color = 750 Pt-Co. The Jartest experiment results showed that the process efficiency of chemical polymer and gum MHY is not so different, with the COD removal efficiencies of 60.3% and 59.7%; the color removal efficiencies of 87.3% and 87.1%; the SS removal efficiencies of 93.2% and 92.6%. There-fore, coagulants obtained from gum MHY can be applied as the alternatives for chemical polymer in the process of treating textile wastewater. / Các ứng dụng chất keo tụ và chất trợ keo tụ hóa học trong xử lý nước thải ngày càng trở nên phổ biến tại Việt Nam. Mặc dù có nhiều ghi nhận về hiệu quả xử lý của chất keo tụ hóa học, phương pháp xử lý này vẫn tồn tại một số nhược điểm như hiệu suất xử lý thấp ở nhiệt độ thấp, nước thải sau khi xử lý còn chứa nhiều hóa chất tiếp tục làm ô nhiễm nguồn tiếp nhận, chi phí xử lý cao và tạo ra nhiều bùn thải. Do đó việc tìm kiếm một phương án xử lý thay thế, chẳng hạn sử dụng polymer tự nhiên, có thể khắc phục những nhược điểm này là rất cần thiết. Nghiên cứu này tiến hành đánh giá hiệu quả sử dụng chất trợ keo tụ sinh học ly trích từ hạt trái Muồng Hoàng yến (Cassia fistula) và chất trợ keo tụ hóa học để xử lý nước thải dệt nhuộm có các thông số ô nhiễm cơ bản: pH = 9,0; COD = 800 mgO2/L, độ màu = 750 Pt-Co. Các thí nghiệm trên bộ Jartest cho thấy hiệu quả xử lý nước thải dệt nhuộm của chất trợ keo tụ gum Muồng Hoàng yến và chất trợ keo tụ hóa học không khác biệt có ý nghĩa với hiệu suất xử lý COD lần lượt là 60,3 và 59,7%; hiệu suất xử lý độ màu là 87,3 và 87,1%; xử lý SS là 93,2 và 92,6%. Kết quả nghiên cứu cho thấy gum hạt Muồng Hoàng yến có thể sử dụng làm chất trợ keo tụ thay thế chất trợ keo tụ hóa học trong xử lý ô nhiễm nước thải dệt nhuộm.

Une fistule artérioveineuse (FAV) est un accès vasculaire permanent créé par voie chirurgicale en connectant une veine et une artère chez le patient en hémodialyse. Cet accès vasculaire permet de mettre en place une circulation extracorporelle partielle afin de remplacer les fonctions exocrines des reins. En France, environ 36000 patients sont atteint d’insuffisance rénale chronique en phase terminale, stade de la maladie le plus grave qui nécessite la mise en place d’un traitement de suppléance des reins : l’hémodialyse. La création et présence de la FAV modifient significativement l’hémodynamique dans les vaisseaux sanguins, au niveau local et systémique ainsi qu’à court et à plus long terme. Ces modifications de l’hémodynamiques peuvent induire différents pathologies vasculaires, comme la formation d’anévrysmes et de sténoses. L’objectif de cette étude est de mieux comprendre le comportement mécanique et l’hémodynamique dans les vaisseaux de la FAV. Nous avons étudié numériquement les interactions fluide-structure (IFS) au sein d’une FAV patient-spécifique, dont la géométrie a été reconstruite à partir d’images médicales acquises lors d’un précédent doctorat. Cette FAV a été créée chez le patient en connectant la veine céphalique du patient à l’artère radiale et présente une sténose artérielle réduisant de 80% la lumière du vaisseau. Nous avons imposé le profil de vitesse mesuré sur le patient comme conditions aux limites en entrée et un modèle de Windkessel au niveau des sorties artérielle et veineuse. Nous avons considéré des propriétés mécaniques différentes pour l’artère et la veine et pris en compte le comportement non-Newtonien du sang. Les simulations IFS permettent de calculer l’évolution temporelle des contraintes hémodynamiques et des contraintes internes à la paroi des vaisseaux. Nous nous sommes demandées aussi si des simulations non couplées des équations fluides et solides permettaient d’obtenir des résultats suffisamment précis tout en réduisant significativement le temps de calcul, afin d’envisager son utilisation par les chirurgiens. Dans la deuxième partie de l’étude, nous nous sommes intéressés à l’effet de la présence d’une sténose artérielle sur l’hémodynamique et en particulier à ses traitements endovasculaires. Nous avons dans un premier temps simulé numériquement le traitement de la sténose par angioplastie. En clinique, les sténoses résiduelles après angioplastie sont considérées comme acceptables si elles obstruent moins de 30% de la lumière du vaisseau. Nous avons donc gonflé le ballonnet pour angioplastie avec différentes pressions de manière à obtenir des degrés de sténoses résiduelles compris entre 0 et 30%. Une autre possibilité pour traiter la sténose est de placer un stent après l’angioplastie. Nous avons donc dans un deuxième temps simulé ce traitement numériquement et résolu le problème d’IFS dans la fistule après la pose du stent. Dans ces simulations, la présence du stent a été prise en compte en imposant les propriétés mécaniques équivalentes du vaisseau après la pose du stent à une portion de l’artère. Dans la dernière partie de l’étude nous avons mis en place un dispositif de mesure par PIV (Particle Image Velocimetry). Un moule rigide et transparent de la géométrie a été obtenu par prototypage rapide. Les résultats expérimentaux ont été validés par comparaison avec les résultats des simulations numériques. / An arteriovenous fistula (AVF) is a permanent vascular access created surgically connecting a vein onto an artery. It enables to circulate blood extra-corporeally in order to clean it from metabolic waste products and excess of water for patients with end-stage renal disease undergoing hemodialysis. The hemodynamics results to be significantly altered within the arteriovenous fistula compared to the physiological situation. Several studies have been carried out in order to better understand the consequences of AVF creation, maturation and frequent use, but many clinical questions still lie unanswered. The aim of the present study is to better understand the hemodynamics within the AVF, when the compliance of the vascularwall is taken into account. We also propose to quantify the effect of a stenosis at the afferent artery, the incidence of which has been underestimated for many years. The fluid-structure interactions (FSI) within a patient-specific radio-cephalic arteriovenous fistula are investigated numerically. The considered AVF presents an 80% stenosis at the afferent artery. The patient-specific velocity profile is imposed at the boundary inlet, and a Windkessel model is set at the arterial and venous outlets. The mechanical properties of the vein and the artery are differentiated. The non-Newtonian blood behavior has been taken into account. The FSI simulation advantageously provides the time-evolution of both the hemodynamic and structural stresses, and guarantees the equilibrium of the solution at the interface between the fluid and solid domains. The FSI results show the presence of large zones of blood flow recirculation within the cephalic vein, which might promote neointima formation. Large internal stresses are also observed at the venous wall, which may lead to wall remodeling. The fully-coupled FSI simulation results to be costly in computational time, which can so far limit its clinical use. We have investigated whether uncoupled fluid and structure simulations can provide accurate results and significantly reduce the computational time. The uncoupled simulations have the advantage to run 5 times faster than the fully-coupled FSI. We show that an uncoupled fluid simulation provides informative qualitative maps of the hemodynamic conditions in the AVF. Quantitatively, the maximum error on the hemodynamic parameters is 20%. The uncoupled structural simulation with non-uniform wall properties along the vasculature provides the accurate distribution of internal wall stresses, but only at one instant of time within the cardiac cycle. Although partially inaccurate or incomplete, the results of the uncoupled simulations could still be informative enough to guide clinicians in their decision-making. In the second part of the study we have investigated the effects of the arterial stenosis on the hemodynamics, and simulated its treatment by balloon-angioplasty. Clinically, balloon-angioplasty rarely corrects the stenosis fully and a degree of stenosis remains after treatment. Residual degrees of stenosis below 30% are considered as successful. We have inflated the balloon with different pressures to simulate residual stenoses ranging from 0 to 30%. The arterial stenosis has little impact on the blood flow distribution: the venous flow rate remains unchanged before and after the treatment and thus permits hemodialysis. But an increase in the pressure difference across the stenosis is observed, which could cause the heart work load to increase. To guarantee a pressure drop below 5 mmHg, which is considered as the threshold stenosis pressure difference clinically, we find that the residual stenosis degree must be 20% maximum.

Le développement récent de l’endoscopie interventionnelle digestive nous a conduit à prendre en charge deux types de pathologies préoccupantes. Il s’agit d’une part des fistules digestives souvent responsables d’une morbi-mortalité élevée et d’autre part des sténoses œsophagiennes après résection tumorale endoscopique étendue. Dans ces deux situations, des phénomènes inflammatoires chroniques conduisent soit à l’absence de fermeture de la fistule soit à une fibrose importante responsable de sténose de l’œsophage. La thérapie cellulaire a déjà été utilisée pour diminuer ces phénomènes inflammatoires et entrainer une cicatrisation. La thérapie tissulaire par cellules souches organisées en construction 3D représente un avantage important en permettant de cibler le site d’action par dépôt direct du feuillet cellularisé. Notre objectif était d’évaluer l’effet thérapeutique de ces nouveaux outils pour fermer les fistules digestives et pour prévenir la survenue des sténoses œsophagiennes. La première étape a consisté a évaluer l’efficacité du traitement par des cellules souches mésenchymateuses provenant de moelle osseuse humaine, marquées puis organisées en doubles feuillets, dans un modèle de fistule entéro-cutanée post-chirurgicale chez la souris nude. L’évaluation clinique et en imagerie (IRM et microscopie confocale) a montré une meilleure cicatrisation avec une augmentation de la microvascularisation et une accélération de la fermeture de la fistule chez les souris greffées. Les effets observés semblent liés à une augmentation précoce de la synthèse des facteurs de réparation (EGF et le VEGF) et des cytokines anti-inflammatoires (TGF-ß2 et IL-10). Après avoir développé un modèle inédit de fistule oeso-cutanée chez le porc grâce à la mise en place par voie endoscopique et chirurgicale de prothèses plastiques entre la lumière œsophagienne et la peau, nous avons évalué l’efficacité thérapeutique d’un gel contenant des vésicules extracellulaires issues de cellules souches isolées du tissu adipeux de porc. Ce gel injecté dans la fistule par voie endoscopique a permis la fermeture des fistules. Enfin, la troisième partie de notre travail a consisté à évaluer l’efficacité de la greffe allogénique de doubles feuillets de cellules souches mésenchymateuses pour prévenir la survenue des sténoses œsophagiennes dans un modèle porcin après dissection sous muqueuse étendue. Il existait une réduction significative du taux de sténose œsophagienne cicatricielle dans le groupe greffé avec une fibrose moins importante. En conclusion, l’effet paracrine antifibrosant des cellules souches mésenchymateuses organisées en feuillets est efficace à la fois pour fermer les fistules entéro-cutanées chez la souris et pour prévenir les sténoses œsophagiennes chez le porc. Un gel avec des vésicules extracellulaires issues des cellules souches a de la même façon un effet cicatrisant anti-inflammatoire permettant la fermeture des fistules œsophagiennes chez le porc. Ces résultats sont très encourageants et posent la question d’une évaluation future chez l’homme. / Recent developments in digestive interventional endoscopy lead us to manage two types of digestive disease. First, it is digestive fistulas associated in many cases with high morbi-mortality; and second is oesophageal stenosis after extended superficial endoscopic resection. In both situations, chronic inflammatory process resulted in delayed or no fistula healing for the first case or oesophageal stenosis due to fibrosis. Cellular therapy has proved to be successful in reducing the inflammatory process and to promote tissue healing. Tissue therapy with 3D construct stem cells represents a major advantage by allowing a direct adaptation on the right place. Our objective was to evaluate the therapeutic effect of new strategy to close the digestive fistula and to prevent oesophageal stenosis. First step was to evaluate the effect of labelled human bone marrow derived mesenchymal stem cells engraftment in the form of double cellsheet in a post-surgical fistula model in nude mice. Clinical and radiological (MRI and probe based confocal microscopy) evaluation showed a better fistula healing with higher microvascularization and a faster fistula closing in grafted mice. These effects appear to be related to an increase production of factors involved in tissue repair (EGF et le VEGF) and anti-inflammatory cytokines (TGF-ß2 et IL-10). We developed an unpublished eso-cutaneous fistula in a porcine model after plastic catheters placement by surgical and endoscopic way between the oesophageal lumen and the skin. We evaluated the therapeutic effect of a hydrogel with extracellular vesicles extracted from porcine adipose derived stem cells. The hydrogel with vesicles was injected into the fistula by endoscopy. Radiological and histological evaluation 15 days after injection showed a fistula tract closure in treated group.The third part of this work was to evaluate the effect of allograft of adipose derived stem cells 3D construct to prevent the stenosis after extended endoscopic submucosal dissection in a porcine model. There was a significant reduction of number and degree of stenosis with decrease fibrosis infiltration in the grafted group.In summary, thanks to their paracrine and antifibrotic effect, the mesenchymal stem cells organised as 3D construct allowed fistula closure in an entero-cutaneous model in mice and prevention of stenosis after extended oesophageal submucosal dissection in a porcine model. Moreover, endoscopic hydrogel and extracellular vesicles injection allowed oesophageal fistula healing in a porcine model. These promising results pose the challenge of future clinical studies.