The non-native Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) has been grown to a limited extent in the southern part of Sweden since the early 1900s. A more extensive use has probably been curtailed by its known susceptibility to damage by frost, pine weevil and other pests. Limited access to vital seedlings of suitable provenances has also restricted its more widespread growth. The need for valuable species that will grow well through ongoing climate change has increased the interest for Douglas fir in Sweden. This thesis addresses a number of important questions relating to the planting of Douglas fir in Sweden: seedling storability, freezing tolerance, timing of bud burst, frost damage and seedling response to site preparation. Seven Douglas fir provenances originating from British Columbia, Canada were used in the experiments and where applicable, compared with a local provenance of Norway spruce (Picea abies (L.) Karst.). The early bud burst of interior Douglas fir provenances, observed both in greenhouse tests and in the field, results in a high risk of damage by late spring frost. This type of damage does not seem, however, to be fatal and does not obviously retard the early growth of seedlings. The difference in growth between coastal and interior provenances was insignificant, but survival was greater for interior than for coastal provenances. All the provenances of Douglas fir studied showed a later development of freezing tolerance of shoots and roots in the autumn than Norway spruce, with the coastal provenances developing such tolerance even later than interior provenances. This could be a contributory cause for the severe damage by winter desiccation observed on seedlings of coastal origin. A thorough site preparation proved to be an effective way to increase survival and root growth. Interior provenances of Douglas fir became ready for storage earlier in autumn than coastal provenances. When freezing tolerance of shoots had increased sufficiently, Douglas fir seedlings could safely be kept in frozen storage using the same procedures used for Norway spruce. The results emphasise the need to gain further knowledge about how the remaining obstacles to establishment of Douglas fir could be reduced with different silviculture methods.

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Lic.-avh. Uppsala : Sveriges lantbruksuniv. / Härtill 2 uppsatser.

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Frost can be detrimental to canola (Brassica napus L.) production. Depending on the severity, the entire field can be killed. Having frost tolerance in canola would benefit growers by allowing them to plant early, utilize early season moisture, and avoid high heat during flowering. However, frost tolerance in canola has not been well studied. A protocol was developed that determined 14 day old seedlings should be acclimated at 4°C for 7 days before being exposed to overnight frost (-4°C) in a small freezing chamber. However, when a larger chamber was used for freezing, the protocol was optimized to -8°C instead. A greenhouse study was conducted on a diverse collection of 231 genotypes and genome-wide association scan (GWAS) was conducted to identify potential genes that were related to frost tolerance or abiotic stress tolerance. Thirty-eight significant single nucleotide polymorphism (SNP) markers were selected based on 10,000 bootstraps and 0.1 percent tail of the empirical distribution. The markers were located on chromosomes A01, A02, A03, A04, A07, A08, A09, A10, C03, C05, C06, C07, and C09. Stepwise regression highlighted a QTL located on chromosomes A02. Another GWAS was done on 147 canola germplasm lines phenotyped under natural conditions. Thirty-eight significant SNPs identified from this study were located on chromosomes A05, A07, A09, C01, C02, C03, C04, C05, C06, C07, and C09. Stepwise regression identified a QTL located on chromosome C04. A protocol was developed to measure the freezing induced electrolyte leakage from leaves of rapeseed/canola. A total of 157 germplasm lines were evaluated for freezing induced (-12°C for 2 h) electrolyte leakage. Thirty-six significant SNPs located on chromosomes A01, A02, A03, A04, A05, A06, A07, A08, A09, A10, C01, C02, C04, C05, C06, C07, and C09 were identified. Stepwise regression identified 10 QTL located on chromosomes A01, A02, A04, A06, A07, C02, C05, C07, C09, and one that could not be assigned. All GWAS studies identified potential genes of interest that were related to frost tolerance, abiotic stress, and transcription factors. / Northern Canola Growers Association

No description available.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniversitet, 2005. / Härtill 5 uppsatser.

Roads are subjected to mechanical loads from the traffic as well as deteriorating mechanisms originating from the surrounding environment and climate. The damage arising is particularly severe during the winter season, when for example raveling, pot holes and cracks can emerge on the surfaces of asphaltic roads. These winter related damages are difficult to characterize and predict, partly due to the complexity of the asphalt material and partly since they cannot be linked to one single phenomenon but several, such as the (long term) existence of moisture, frost damage and frost heave, low temperature cracking and the embrittlement of the mastic at low temperatures. Further adding to the complexity is the combination of these phenomena which may accelerate the emergence and evolution of the damage mechanisms. This licentiate research project is mainly focusing on the emergence and development of frost damage in the asphalt layer but will include the effect of other damage mechanisms in its continuation. The goal of the project is to develop a multiscale model able to predict the damage development in an asphalt pavement during a desired period of time, to enhance maintenance predictions as well as pavement design choices. This licentiate thesis is the first part of this project and aims to lay the foundation of the multiscale model. To achieve this, a micromechanical model of frost damage in asphalt mixtures has been developed. This model couples the moisture and mechanical damage happening on the short and long term, caused by the infiltration of moisture and the expansion of water turning into ice during temperature drops. Both possible adhesive damage in the mastic-aggregate interface and cohesive damage in the mastic is included. In addition to the developed micromechanical model, this thesis presents the overall concept for the formulation of the multiscale model as well as discusses about its motivations and advantages. / Vägar utsätts både för mekaniska laster från trafiken som kör på vägen samt för nedbrytande mekanismer härstammande från den omgivande miljön och klimatet. Skadorna som uppstår är särskilt stora under vintern, då till exempel stensläpp, potthål och sprickor kan uppstå på ytan av asfalterade vägar. Dessa vinterrelaterade skador är svåra att karakterisera och förutsäga, delvis på grund av det komplexa beteendet hos asfalt och delvis eftersom de inte härstammar från enbart ett fenomen utan flera, såsom existensen av fukt i asfalten (på lång sikt), frostskador, tjällyft, sprickbildning på grund av låg temperatur samt försprödningen av asfalt som sker vid låga temperaturer. Vidare påverkar dessa skademekanismer varandra vilket kan accelerera skadebildningen och utvecklingen, vilket ytterligare ökar komplexiteten. Detta licentiatforskningsprojekt fokuserar till största delen på uppkomsten och utvecklingen av frostskador men kommer även inkludera effekten av andra skademekanismer i dess fortsättning. Målet med detta forskningsprojekt är att utveckla en multiskalemodell som kan förutspå skadeutvecklingen i en asfaltsväg under en önskad tidsperiod, för att förbättra både underhållsprognoser samt designval. Denna licentiatuppsats är den första delen i detta projekt och syftar till att lägga grunden till multiskalemodellen. För att uppnå detta har en mikromekanisk modell av frostskador i asfalt utvecklats. Denna modell kopplar ihop fuktskadan och den mekaniska skadan som sker både på kort och lång sikt, orsakad av infiltrationen av fukt och expansionen av vatten som omvandlas till is vid sjunkande temperatur. Modellen inkluderar de möjliga skadorna som uppstår i både mastics och gränsskiktet mellan mastics och stenmaterialet. Utöver den utvecklade mikromekaniska modellen presenterar denna uppsats det övergripande konceptet för formuleringen av multiskalemodellen samt diskuterar dess motivering och fördelar. / <p>QC20190515</p>

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