The Wheatonvilie Consolidated Oil Field lies in Onion and Barton Townships of Gibson County, Indiana. Oil is produced from a sandstone reservoir commonly referred to the "Jackson Sand". The Jackson Sandstone is here informally adopted as member rank of Big Clifty Formation. The Stephen sport Group includes the Big Clifty and is Late Mississippian (Chesterian) in age.Within the area of the field, the Big Clifty Formation has been mapped between the underlying Beech Creek Limestone and the overlying Harvey Limestone. The lower contact of the Big Clifty appears to be sharp. The upper contact of the Big Clifty intergrades with at least one thin limestone tongue of Haney which pinches out laterally into the uppermost shales of the Big Clifty.The Big Clifty Formation includes sandstone, siltstone, shale, and mudstone with minor amounts of sandy limestone. A typical sequence from top to bottom includes:dark gray shale; thin red mudstone; gray shale; limey siltstone; very fine-grained white sandstone interbedded with gray shale; well sorted, fine-grained, white sandstone; and thin black shale. The percentage of sandstone within the Big Clifty Formation varies laterally significantly.The thickness of the Big Clifty Formation ranges from 64 feet to 97 feet. The formation dips to the southwest at a rate of approximately 29 feet per mile and lies 940 feet below the surface within the northeast portion of the study area.The areas containing high concentrations of sandstone form two elongated trends. The sandstone trends strike N35°E. The axes of the trends lie approximately 1 mile apart. The sandstone bodies are approximately 3/4 of a mile across and 5 miles long. These sand bodies range in thickness from 20 feet to 64 feet.The sandstone bodies may represent tidally influenced shallow marine offshore bars. Cross laminations and very fine grained clastics indicate a low velocity aqueous environment. The elongate shape of the sands indicate bar deposits and the sandy limestones indicate a marine environment.

Thesis (D. Min.)--Abilene Christian University, 1994. / Includes abstract. Includes bibliographical references (leaves 208-218).

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / To present dissertation it is the result of an investigation on the conception behaviorist of the language of Quine, tends as pretension your exposition and defense, alleging your permanence in elapsing of all the quinean works. The importance of this work is due to the fact that many critics were made Quine with regard to your linguistic perspective. Among the critics, what promotes contusing objections, it is Noam Chomsky. This alleges that Quine suffers from inconsistency and incoherence when proposing your language conception, and he still affirms that Quine abandons the behaviorism in your last works in favor of a mentalism. To accomplish the defense of Quine front to the accusations of Chomsky it was had as reference Roger Gibson, which elaborated a defense of the position of Quine, that however it was not very clear. This way, in this dissertation it is made an analysis not only of the critics of Chomsky, but also of the defense of Gibson in the search of offering a better explanation of the conception of Quine. It is sustained that the program behaviorist quinean is presented mainly in Word and Object, published in 1960, but it stays until your last publications. In these some are observed explanations and amplifications than it was already presented in 1960, but non incoherences. The sustentation of your conception is found in your rejection to the semantics traditional mentalist that accepts intension concepts as mental entities when correlating words with ideas. Quine defends that the linguistic meaning is a property of the behavior and, for this reason, not meeting differentiates comportamental, it is not possible to allege semantic difference. Quine assumes, then, that a perspective behaviorist is necessary for the study of the acquisition of the language, even if it is not enough. Besides, the defense of a behaviorism is not only important for your semantic aspect, but also epistemological, because Quine defends that the only possible road to understand the relationship between theory and world is, if before, the subject psychogenetic of "as it is acquired the language" it is answered. Starting from that, the conception behaviorist ends up assuming a central paper in the philosophy of Quine and, for the which, a lot of subjects are answered. / A presente dissertação é o resultado de uma investigação sobre a concepção behaviorista da linguagem de Quine, tendo como pretensão a sua exposição e defesa, alegando a sua permanência no decorrer de todos os trabalhos quineanos. A importância deste trabalho se deve ao fato de que muitas críticas foram feitas a Quine com respeito a sua perspectiva linguística. Dentre os críticos, o que promove objeções contundentes, é Noam Chomsky. Este alega que Quine sofre de inconsistência e incoerência ao propor sua concepção de linguagem, e ainda afirma que Quine abandona o behaviorismo nos seus últimos trabalhos em favor de um mentalismo. Para realizar a defesa de Quine frente às acusações de Chomsky teve-se como referência Roger Gibson, o qual elaborou uma defesa da posição de Quine, que, entretanto, não ficou muito clara. Desta forma, nesta dissertação é feita uma análise não só das críticas de Chomsky, mas também da defesa de Gibson na tentativa de se apresentar um melhor esclarecimento da concepção de Quine. Sustenta-se que o programa behaviorista quineano é apresentado principalmente em Word and Object, publicado em 1960, mas permanece até suas últimas publicações. Nestas são observados alguns esclarecimentos e ampliações do que já era apresentado em 1960, mas não incoerências. A sustentação da sua concepção é encontrada na sua rejeição à semântica mentalista tradicional que aceita conceitos intensionais como entidades mentais ao correlacionar palavras com ideias. Quine defende que o significado linguístico é uma propriedade do comportamento e, por esta razão, não se encontrando diferença comportamental, não é possível alegar diferença semântica. Quine assume, então, que uma perspectiva behaviorista é necessária para o estudo da aquisição da linguagem, mesmo que não seja suficiente. Além disso, a defesa de um behaviorismo não é importante somente pelo seu aspecto semântico, mas também epistemológico, visto que Quine defende que a única via possível para entender a relação entre teoria e mundo é, se antes, a questão psicogenética de como é adquirida a linguagem é respondida. A partir disso, a concepção behaviorista acaba assumindo um papel central na filosofia de Quine e, pela qual, muitas questões são respondidas.

The question of gender, specifically gender identity, is prominent in today’s society. It is highly debated and through the development of queer theory it is gaining more academic recognition. However, there is a gap regarding representation of the gender-queer identity of one contemporary poet, Andrea Gibson. Gibson provides a much needed perspective and voice in society and scholarly debates. This is why this essay uses queer theory along with Kate Bornstein and Judith Butler to examine three poems by Gibson, “Swing-Set, “The Jewelry Store” and “A Genderful Pep-Talk for my Younger Self”. The essay analyses the ways Gibson, through poetry, formulates a gender-queer identity and thus questions the generic gender binary system.

Since William Gibson includes a Rastafarian Enclave and a theological compass in <i>Neuromancer</i>, this can be used to examine the troublesome natures of the two Artificial Intelligences, Wintermute and Neuromancer. The Rastafarian's beliefs and interpretations of Babylon and Zion, the oppressors and the liberated, add political significance to the Tessier-Ashpool's and their enslaved Artificial Intelligences. Since the Artificial Intelligences are both created of Babylon the Tessier-Ashpools and also wanting to be free of them, they are something outside of both. In the Artificial Intelligences, then, Gibson collapses the straightforward dichotomy of Babylon and Zion.

Since William Gibson includes a Rastafarian Enclave and a theological compass in <i>Neuromancer</i>, this can be used to examine the troublesome natures of the two Artificial Intelligences, Wintermute and Neuromancer. The Rastafarian's beliefs and interpretations of Babylon and Zion, the oppressors and the liberated, add political significance to the Tessier-Ashpool's and their enslaved Artificial Intelligences. Since the Artificial Intelligences are both created of Babylon the Tessier-Ashpools and also wanting to be free of them, they are something outside of both. In the Artificial Intelligences, then, Gibson collapses the straightforward dichotomy of Babylon and Zion.

Thesis (M.A.)--Georgia Southern University, 2004. / "A thesis submitted to the Graduate Faculty of Georgia Southern University in partial fulfillment of the requirements for the degree Master of Arts." ETD. INDEX WORDS: William Gibson, Neal Stephenson, Neuromancer, Snow crash, science fiction, cyberpunk, cyberspace, metaphysics, cyberculture, transrealism. Includes bibliographical references (p. 71-73).

The ecological movement in the psychology of perception, founded by James J. Gibson, hold that traditional approaches to perception are based upon certain fundamental mistakes. The chief one, ecological theorists claim, is that perceptual information pickup consists of the application of certain cognitive processes to sensory input which is not specific to features of organisms' environment. Gibson's fundamental claim is that perception does not require the processing of some form of sensory input. In this sense, the ecological approach is said to be a theory of direct perception. An important debate over the Gibsonian view concerns the question of whether or not perceptual information pickup without cognitive processing is a coherent notion. Among the more recent writers who claim that the ecological view will not work as it stands are Jerry A. Fodor and Zenon W. Pylyshyn. They claim, essentially, that Gibson's approach has no means for accounting for intentionality. Fodor and Pylyshyn are answered by four prominent Gibsonians who claim such criticisms are utterly baseless. These ecological theorists, Michael Turvey, Robert Shaw, Edward Reed, and William Mace endeavour to show how their approach can indeed account for intentionality. This debate between Fodor and Pylyshyn on the one hand, and Turvey, Shaw, Reed, arid Mace on the other is a perfect example of the kinds of misunderstandings that have arisen between Gibsonians and proponents of traditional view. In this thesis, I supply a detailed description of Gibson's model as it relates to the issue of how intentionality could survive perception without processing. Fodor and Pylyshyn's understanding and assessment of the Gibsonian position will then be examined. Although these defenders of traditional views have, some important concerns,.they also seem not to have a proper grasp of some Gibsonian concepts. In particular, Fodor arid Pylyshyn have an unsatisfactory grasp of the notion of an invariant. There are more serious misunderstandings evident in the response to Fodor arid Pylyshyn given by Turvey et al. I point out that these ecological theorists have difficulties with philosophical terms and theories they employ in defense of Gibson. As a result of evident confusions over notions of intension, extension, and property, arid confusions over the nature of Fred I. Dretske's theory of natural laws and Hillary Putnam's theory of natural kinds, Turvey et al do not manage to show how Gibson's approach could account for intensionality. I conclude by suggesting that the ecological approach nevertheless is compatible with the idea, of analyzing perceptual information pickup in terms of behaviour, or dispositions to behave. On such an interpretation, the ecological approach is similar in many important respects to the D.M. Armstrong's philosophical theory of perception. The comparison provides ecological theorists with a precedent as well as philosophical model to consult in order to better, understand the philosophical language and terminology. On the other hand, the comparison with Armstrong provides philosophers of perception with a means for approaching Gibson's view and the problems with which it will he confronted. / Arts, Faculty of / Philosophy, Department of / Graduate

Non-coding DNA in the human genome is widely studied to investigate its effect on coding DNA and gene expression. Non-coding DNA contains cis-regulatory elements that influence transcription of genes upstream, downstream, or nearby. These regulatory elements have largely been studied as enhancers that promote the transcription of genes. To explore these regulatory elements as silencers, we chose validated bifunctional elements to study their silencing capability and their chromatin markers. We used chromatin immunoprecipitation methods with dCas9 to target these elements using single-guide RNAs (sgRNAs). We experimented with various cloning methods to insert dCas9 into the pUAS vector. We initially planned to use the Gibson Assembly method, but after no success, we tried site-directed mutagenesis and traditional cloning with restriction enzymes. We were able to successfully insert dCas9 into the pUAS vector with traditional cloning, and we were then able to inject the construct into Drosophila melanogaster. We designed sgRNAs to target desired elements of DNA that we chose to study as cis-regulatory elements. The sgRNA sequences were cloned into the pCFD5 vector and injected into another line of flies. The transgenic flies containing the pUAS/dCas9 plasmid will then be crossed with the flies containing the pCFD5/sgRNA to develop offspring that express the target elements and could undergo chromatin pulldown to examine the bifunctional regulation of these DNA elements in cells. Results from a quantitative PCR (qPCR) assay on Drosophila expressing the cloned pUAS vector with dCas9 and a sgRNA for the white gene showed chromatin pulldown efficiency and successful transfection. The Drosophila chromatin targeted by the sgRNAs will be pulled down, solubilized, and then analyzed on a western blot to screen for chromatin modifications, primarily histone modifications. We can then identify chromatin markers associated with elements when they act as silencers in the mesoderm versus when they act as non- mesodermal enhancers. We can also determine if the silencer acts by interacting with a promoter or with an enhancer to repress gene expression. If ENCODE can profile the data found in this project, the chromatin markers can act as a predictive tool for the identification of silencers.

Le système baculovirus / cellules d’insectes est un outil performant pour la production de vecteurs AAV recombinant pour la thérapie génique. Cette production nécessite que, les gènes rep et cap de l’AAV ainsi que le transgène encadré par les ITR, soient codés dans le génome du baculovirus AcMNPV. L’utilisation de cassettes de recombinaison pour intégrer ces gènes provoque à grande échelle une certaine instabilité au sein des 134Kb du génome. Pour pallier à ce phénomène, une nouvelle stratégie d’intégration de gènes a été mise en place. Celle-ci doit permettre notamment d’éviter la présence de cicatrices de recombinaison et de modifier le génome du bacmid d’AcMNPV. Basée sur la technique de Gibson Assembly, nous avons tenté d’assembler de novo le génome du baculovirus d’AcMNPV à partir de fragments PCR chevauchants. Nous avons réussi à pré assembler en 4 parties la totalité du génome. Celles-ci ont permis d’insérer le gène de la GFP comme gène d’intérêt mais également d’éliminer le gène de résistance antibiotique et le Mini-F réplicon, facteur d’instabilité en cellules d’insecte. Plusieurs clones obtenus ne contiennent aucune mutation. Cette technique pourrait être appliquée à la production de vecteurs AAVr. / The baculovirus / insect cell system allows AAV vector production for gene therapy purposes. Current baculoviruses used for the production of rAAV vectors are a bottleneck for Scaling up production. Indeed the use of recombination boxes to insert the genes brings instability to the134kb genome. To avoid this, a new gene integration strategy has been implemented. In this work, we have assayed the full assembly of AcMNPV´s genome using the Gibson Assembly technic. PCR fragments covering the totality of genome and able to assemble through overlapping terminal regions have been pre-assembled in 4 segments. The finally assembly should contain the eGFP gene used has gene of interest along with replacement of the Mini-F replicon by polyhedrin gene. This feasibility study has shown that we could obtain segments without any mutation. Final assembly is still on-going. This technology should be applied next for the generation of baculovirus used for the production of rAAV vectors. This marker less combination method should solve problems of genome instability.