No description available.

The political discourse that has emerged as a consequence of establishing a European regulatory framework for GMOs has not been without problems. This dissertation addresses the political and regulatory challenges created by the development and use of genetically modified organisms (GMOs) in the EU. The underlying hypothesis in the dissertation is that the emergence of a European policy in the field of GMOs has appeared through new reflexive forms of governance. The aim of this research is to understand how these reflexive forms of regulation have emerged and operate within the EU, with a particular focus on the two GMO directives 90/220/EEC and 2001/18/EC. However, the study scrutinises the regulatory regimes from the 1970s onwards by investigating how the regulatory framework regarding GMOs has been developed and implemented in the EU. This is done through an analysis of the notion of ‘risk’ and the ‘precautionary principle’ since these concepts have been at the forefront of the GMO regulation debate. The empirical approach focuses on how the European Commission, the European Parliament and the European Council as well as other actors and institutions articulate ambivalence, interpretations and uncertainties in the decision-making processes regarding regulative measures for GMOs, with an accent on risk and the precautionary principle. The main empirical material has been documents concerning the inter-institutional process involved in the regulatory process of making the two directives. The analysis indicates that during the process of implementing GMO regulations, new steering strategies have appeared within the EU’s decision-making institutions when the objective of the regulation has taken centre stage in political and scientific controversies. / Den politiska diskursen som har uppstått som en följd av upprättandet av ett europeiskt regelverk för GMO har inte varit utan problem. Denna avhandling behandlar de politiska utmaningar som alstrats av skapandet och tillämpningen av genetiskt modifierade organismer (GMO) inom EU. Den underliggande hypotesen i avhandlingen är att framväxten av en europeisk politik på området för GMO har uppstått genom nya reflexiva regleringsformer. Syftet med denna forskning är att förstå hur reflexiva regleringsformer har uppkommit och opererar inom EU, med särskilt fokus på de två GMO direktiven 90/220/EEG och 2001/18/EG. Studien analyserar lagstiftning från 1970-talet och framåt genom att undersöka hur lagstiftningen om genetiskt modifierade organismer har utvecklats och implementerats i EU. Detta görs genom en analys av begreppen ‘risk’ och ‘försiktighetsprincipen’ eftersom dessa begrepp har varit centrala för debatten om GMO lagstiftningen. Den empiriska metoden fokuserar på hur Europeiska kommissionen, Europaparlamentet och Europeiska rådet samt andra aktörer och institutioner har uttryckt ambivalens, tolkningar och osäkerhet i beslutsfattandet gällande reglerings åtgärder för genetiskt modifierade organismer, med tonvikt på risk och försiktighetsprincipen. Det huvudsakliga empiriska materialet är dokument gällande den interinstitutionella processen som ägde rum när de två direktiven skapades. Analysen visar att implementeringen av GMO lagstiftningen har skapat nya styrningsstrategier i synnerhet i de fall där ändamålet med lagstiftningen har varit föremål för politiska och vetenskapliga kontroverser.

Uncontrolled spreading of genetically modified (GM) plants is one of the main concerns about their cultivation. Inducible RNA interference against an essential gene could be a tool for control of GM plants. After spraying with a chemical inducer, the essential gene will be silenced so the treated GM plant will die. For testing this strategy we chose two key enzymes of nitrogen metabolism, glutamate synthase (GOGAT) and glutamine synthetase (GS). GS processes ammonium ions into glutamine, then GOGAT transfers the amide group from glutamine to 2-oxoglutarate to form two glutamates. GS/GOGAT cycle is the main pathway for assimilation of ammonium ions, which could be toxic to plants in a higher concentration. Disruption of ammonium assimilation during photorespiration causes a strong inhibition of photosynthesis. The aim of this work was to describe the effects of silencing GOGAT and GS genes in Arabidopsis thaliana. To induce silencing, RNAi hairpin constructs under a control of constitutive or estradiol-inducible promoter were prepared. In selected independent transformants with the inducible hairpin against GOGAT, chlorosis and reduced growth were observed after the estradiol treatment in in vitro conditions. However, the spraying with estradiol was tricky, at the whole plant level, the induction of...

Da oltre vent’anni l’Unione Europea (UE) è protagonista di un acceso dibattito circa l’orami vasta adozione di OGM in agricoltura. Laddove la maggior parte dei paesi Nord e Sud americani ha già largamente introdotto tali colture, l’UE è invece in forte ritardo, complice una legislazione basata sul Principio di Precauzione. Queste discrepanze nei i processi legislativi hanno portato a galla alcune problematiche di carattere commerciale: primo, la riduzione delle disponibilità di materia prima non-OGM costituisce un problema per la stabilità delle filiere ad essa dedicate; secondo, il commercio di prodotti convenzionali è compromesso dalla possibilità di riscontrare varietà OGM non approvate in UE all’interno delle partite provenienti da paesi terzi. In ultimo, data l’ampia diffusione di OGM nelle Americhe, è lecito porsi il quesito di come tale tendenza abbia influito sui prezzi delle materie prime agricole. In questa tesi si cercherà di analizzare queste tematiche. Per prima cosa, ci occupiamo di capire come la filiera della soia non-OGM italiana sia organizzata e gestita in modo da minimizzare i rischi di presenza avventizia. La conclusione è che le forme di governance ibride garantiscono la migliore forma di gestione. Successivamente ci domandiamo se le diversità legislative in materia di OGM abbiamo un impatto sull’import Europeo di mais e soia. Utilizzando l’analisi della domanda, riscontriamo che i paesi esportatori competono o sul prezzo o in base alla disponibilità stagionale di prodotto. In ultimo, utilizzando serie storiche, analizziamo il ruolo del tasso di adozione di soia OGM sui prezzi reali; ciò che osserviamo indica che questo ha un effetto deflattivo di breve periodo, ma di scarso peso. / The debate on the increasing adoption of GMOs in agriculture has been in vogue for the last twenty years. Whereas most North and South American countries have largely adopted GMOs, the European Union (EU) has not. Since the EU legislation is based on the Precautionary Principle, the introduction of new GM varieties has been slow. These discrepancies put forward some fundamental issues: first, the decreasing availability of non-GM raw materials poses the sustainability of these supply chains at risk; second, the trade of conventional products is undermined by the possible occurrence of unauthorized GMOs in overseas. Last, the wide adoption of GM crops in North and Latin America poses the question of what effect the spreading of biotechnology in agriculture has exerted on market prices. In this thesis, we try to answer these three research questions. First of all, we investigate how the Italian supply chain for non-GM soybean meal is framed and managed in order to reduce the risk of adventitious presence. We find that hybrid organizations represent the best governance form. Next, we assess the role of legislative diversities (regarding GMOs) on EU import decisions through import demand analysis. We conclude that competition among exporters is solely based on price and seasonality. Last, we disentangle the role of GMOs adoption on the variability of US soybean prices. Results indicate that a higher rate of adoption reduces real soybean market prices, yet the effect is short lived and not much relevant.

This diploma thesis is focused on topic of genetically modified organisms and their use in the practical sectors of human life. Theoretical part of the thesis defines general terms GMO, plasmid, genetic engineering, biotechnology. It also records historical milestones relating to the problematic, deals with individual techniques of genetic engineering and briefly states legislative procedures in context of dealing with GMO. It gives examples of transgenic organisms and summarizes advantages and disadvantages of their use.Practical part of the thesis contains educational program called "Genetically modified organisms", which was conceived by the author and includes a draft of a lesson inclusive of teaching materials - powerpoint presentations, worksheets, interactive worksheets, auxiliary text for teacher and written preparation. Research part deals with high school students change of view about using GMOs after completing the educational program. Due to analysis was proven that most of the attitudes and knowledge about GMO was changed after completing the educational program (for example in issues of willingness to consume GM food and animal products, perception of advantages and disadvantages etc.) Data was still unchanged in questions which cannot be affected by the program (control of food packaging or...

<p>An application of biotechnology that has been rapidly matured under the last ten years is genetically modified food. The deliberative release of GMO faces the challenge of complying with sustainable development and implies a precautionary approach to all possible risk involved. This study purpose is to investigate the problems of risks concerning deliberative release of GMO and to define the question of responsibility. These two themes, risk and responsibility, are discussed in relation to society, citizens, corporations andscience. A more profound understanding of the relation between risk and responsibility in the GMO context could contribute to the sensitivity and deliberation in bio-politics, so it better can cope with democratic governance, public debate and risk deliberations.</p><p>Politicians and other decisions-makers have a responsibility to assure that they have sufficient knowledge and understanding for the issue at hand before taking any decision. A responsible bio-politics departs from the precautionary principle in decisions making, gaining knowledge in dialogue with concerned GMO actors and tries to correspond to sustainable development. Hence, knowledge and understanding is needed which are reached in dialogue with other parties in order to allowed values, attitudes and knowledge to be deliberate more extensively.</p>

An application of biotechnology that has been rapidly matured under the last ten years is genetically modified food. The deliberative release of GMO faces the challenge of complying with sustainable development and implies a precautionary approach to all possible risk involved. This study purpose is to investigate the problems of risks concerning deliberative release of GMO and to define the question of responsibility. These two themes, risk and responsibility, are discussed in relation to society, citizens, corporations andscience. A more profound understanding of the relation between risk and responsibility in the GMO context could contribute to the sensitivity and deliberation in bio-politics, so it better can cope with democratic governance, public debate and risk deliberations. Politicians and other decisions-makers have a responsibility to assure that they have sufficient knowledge and understanding for the issue at hand before taking any decision. A responsible bio-politics departs from the precautionary principle in decisions making, gaining knowledge in dialogue with concerned GMO actors and tries to correspond to sustainable development. Hence, knowledge and understanding is needed which are reached in dialogue with other parties in order to allowed values, attitudes and knowledge to be deliberate more extensively.

This study was carried out to analyze tomato samples and tomato seeds, purchased from different food markets of Turkey randomly, for the presence of genetic modification by using PCR method as it allows more specific detection. The DNAs of collected samples were isolated according to CTAB DNA extraction protocol and also with extraction kits. Screening tests of tomatoes were done by targeting 35S promoter, NOS terminator and NptII kanamycin resistance gene with eight different primer sets. Real time PCR is used to confirm 35S and NOS positives results obtained from conventional PCR. In this study, it was observed that 14 out of 35 seed samples, and 14 out of 40 fresh tomato samples which were screened had at least one transgenic element of 35S promoter, NOS terminator and NPTII kanamycin resistance gene indicating the possible presence of genetic modifications. After screening, gene specific studies were carried out for PG, sam-k indicating F type ripening delayed tomato and the 35 1 N lines respectively and cry1Ac genes inserted in 5345-1 insect resistant tomato line. PG and sam-k specific primers were not amplified in any of the samples investigated whereas 18 out of 75 samples were cry1Ac positive and 1 out of 75 samples was sam-k positive. Positives were confirmed by sequence analysis. Additionally, construct specific primers specific to 5345-1 and 35 1 N lines were designed. PCR amplicons indicate the existence of the construct sequence. In order to verify the results, PCR products were sent to sequence analysis

Diploma thesis Genetically modified foods discusses about genetically modified foods in the Czech Republic, European Union (EU) and worldwide. The aim of the thesis was to investigate the awareness of Czech and Austrian students and citizens about geneti-cally modified foods and also to highlight the pros and cons of genetically modified foods. The method of literary research and comparative method was used to evaluate the current state of genetically modified food. In the experimental part of the thesis a brainstorming method and SWOT analysis were used to determine the main advantages and disadvantages associated with genetically modified foods. Information on the atti-tudes of interest groups was collected through a questionnaire survey. The results show that the awareness and attitude of the general public and students varies within the Czech Republic and Austria.

In 1991, India adopted neoliberalism, a system of political economic practices that promotes private property and free trade, as its political and economic system to promote development in their country. India's neoliberal reform has created issues surrounding human development, resource accumulation, and power struggles. Eleven years later, in 2002, Bt cotton was introduced to the Indian agricultural sector. This research examines how the genetically modified organism Bt cotton is being used to commodify nature in the context of agriculture under neoliberalism. The research focuses on the dispossession of the rural farmers through the commodification of agriculture using Bt cotton. Dispossession of the rural farmers happen through the implications that arise from the commodification of nature. Through Marxist theory of primitive accumulation, this research analyzes accumulation by dispossession and how it neglects the working class and its struggle in rural India. Through this examination, the research will argue alternatives to the dispossession of the working class and the commodification of nature through Bt cotton. Dispossession, in this research, is examined both through working class, but also through the dispossession of biodiversity. Through the loss of biodiversity, the rural farmers are becoming dispossessed from a more sustainable environment. Along with these goals, the research will also incorporate themes of food security through changing landscape of agriculture due to the incorporation of Bt cotton. This research argues the contradictions that are presented through the commodification of agriculture under neoliberalism and provide a contribution to social justice literature, and our understanding of the relationship between technology and the commodification of nature.