Stoats are introduced mammalian carnivores implicated in the decline of several of New Zealand�s endemic species. Most research into stoats in New Zealand has focused on beech forest habitat, especially in years of peak stoat abundance following heavy beech seedfall and peak cohorts of mice. In New Zealand, alpine grasslands occur above the altitudinal limit of beech forest (900-1000 m a.s.l.). Although previous research has shown stoats to be present there, little is known about the ecology of stoats in alpine grasslands. This research aimed to test whether alpine grasslands were a marginal habitat occupied by surplus stoats that had spilled over from beech forest populations, i.e. a sink habitat. The alternative is that alpine grasslands are a desirable habitat deliberately exploited by stoats. This question was answered using mark-recapture, radio-tracking, diet analysis and a food addition experiment. Another objective was to determine whether nest survival is higher in alpine grassland compared to beech forest and whether stoats are likely to be a frequent predator of ground nests in alpine grasslands relative to other introduced mammals that inhabit them. If nest survival is higher in alpine grassland then alpine grasslands may be a refuge from predation. However, if it is not then it is important for management to know the relative risk posed by stoats compared with other predators. An artificial nest experiment was used to answer these questions. This research was undertaken during two years of low to intermediate beech seedfall and therefore provided an opportunity to look at the ecology of stoats in a New Zealand National Park outside years of peak abundance. The principal study site for this research was the Borland Valley, Fiordland National Park. Compositional analysis showed that stoats in alpine grassland selected for it over adjacent beech forest. The range cores of these stoats were high up in alpine grassland away from the ecotone with beech forest. Stoats occurred at similar densities in alpine grasslands as they did in beech forest and observed survival was similar between the two habitats (with the exception of 2004 when it may have been higher in alpine grassland). The most frequent prey of stoats inhabiting beech forest were birds and mice. Although stoats in alpine grasslands also ate birds and mice their most frequent prey were ground weta and hare. Food addition appeared to cause diet switching but did not reduce the distances moved by stoats, suggesting that other factors may be more important in regulating their summer home range size in alpine grasslands. All of these factors lead to the conclusion that alpine grasslands in the Borland are not a marginal habitat for stoats, but may instead be a desirable one. Artificial nests had a higher probability of survival in alpine grassland compared to adjacent beech forest, but survival was too low to support the idea that alpine grasslands are a refuge. Stoats were the most frequent predator of artificial nests in both habitats, but 95 % confidence intervals overlapped the predation rate by possums, which was also high. These findings illustrate the need for a comprehensive landscape approach to stoat control in montane National Parks, for two reasons: 1) endemic biodiversity in alpine grasslands may be under threat from stoat predation, 2) alpine grasslands may act as a source for dispersing stoats that reinvade lowland stoat control areas. In the absence of heavy beech seedfall and peak mouse abundance, stoats occurred at densities of around 1 km⁻� in both habitats and there was recruitment into these populations. This raises the important question: What regulates the distribution and abundance of stoats in years of low beech seedfall and low mouse abundance? In these years birds, ground weta and hares may be as important as mice are in years of peak abundance following heavy beech seedfall.

Agriculture is the principal land use throughout Europe and agricultural intensification has been implicated in large reductions in biodiversity, with the negative effects on birds particularly well documented. The lapwing (Vanellus vanellus) is one such species where changes in farming practices has reduced the suitability and quality of breeding habitat, leading to a drop in population size that has been so severe as to warrant its addition to the Red List of Birds of Conservation Concern in the UK. Lowland areas, where agricultural intensification has generally been most pronounced, have been worst affected, however, more recently declines in marginal upland areas, previously considered refuges for breeding wader populations, have been identified. An upland livestock farm in Stirlingshire that uses an in-bye system of fodder crop management and has unusually high densities of breeding lapwings provides a basis for this project to test causal hypotheses for the decline of upland lapwing populations and to identify potential conservation management solutions. Specifically this farm plants a forage brassica in an in-bye field for two consecutive years, followed by reseeding with grass and seven, out of sixteen, in-bye fields have undergone this regime at the study site since 1997. Fields that had undergone fodder crop management supported almost 60% more lapwings than comparable fields that had not previously been planted with the fodder crop. Lapwing density was highest in the year after the fodder crop was planted, once it had been grazed, which results in a high percentage of bare ground, likely to be attractive to nesting lapwings. Lapwing densities remained above that which occurred in fields that had not undergone fodder crop management for a further four years after the field had been returned to grass. The effect of management on lapwing food resources and nesting structure was tested through a field experiment; liming increased the abundance of Allolobophora chlorotica, an earthworm species that was associated with chick foraging location at the study site, suggesting that lapwings may benefit from liming conducted as part of fodder crop management. The relationship between lapwings and soil pH is further explored across 89 sites on mainland Scotland, using soil property data to improve the predictive power of habitat association models, something which has not previously been done for any farmland bird. Adding soil and topographical data to habitat models, based on established relationships between breeding lapwings and their habitat, improved model fit by almost 60%, indicating that soil properties influence the distribution of this species. The density of breeding lapwings was highest at higher altitude sites, but only when the soil was relatively less peaty and less acidic, providing further support for the hypothesis that agricultural liming benefits lapwings. In addition to assessing the conservation benefit of fodder crop management, the economic costs are also considered. Fodder crop management provides a source of livestock fodder in the autumn and winter during a period when forage demands outstrip grass growth, and ultimately improves the grazing quality of the grass that is replaced; this system currently operates outside of any agri-environment scheme (AES). However, at the study site, planting of the fodder crop and grass is delayed to avoid agriculture operations during the breeding season, which reduces yield and hence profitability. An initial estimate of £200 ha-1 is suggested as an incentive to encourage wider adoption of fodder crop management in a “lapwing friendly” manner, although further work is required to determine if this payment level is appropriate and the current method of AES implementation may limit the suitability of fodder crop management as an AES. The results indicate that agricultural liming could benefit breeding lapwings in pasture fields where soil pH falls below pH 5.2, by increasing earthworm abundance. Where soil pH is below pH 5.2, liming should provide a cost effective mechanism for farmers to improve grass yields. Regular soil testing and liming in response to low pH, within improved or semi-improved grassland fields, where management activities such as use of nitrogen fertiliser can contribute to soil acidification, should be advocated to farmers in marginal areas as a mechanism for improving grass productivity whilst potentially benefitting breeding lapwing and other species where earthworms contribute significantly to their diet.

The current literature shows that global climate is changing with temperatures generally increasing, precipitation patterns becoming less predictable and extreme weather events becoming more frequent. However, the literature is often unclear not only about how changes in temperature will affect soil processes but even about how soil temperatures themselves are changing. This thesis has found that soil temperatures over recent decades have increased at rates comparable to air temperatures (average mean of 0.71 in soil and 0.93 °C in air over the total length of the data sets used). There were differences in seasonal trends between soil and air, for example, winter air temperatures increased twice as quickly as spring air temperatures whereas in soil winter and spring temperatures were increasing at similar rates. This highlights potential problems for predicting how soil functions such as biogeochemical cycling will respond to realistic temperature change. In order to assess the effects of changing soil temperatures on particular reactions involved in soil Nitrogen cycling incubation experiments, both short and longer term in the laboratory as well as soil warming in the field were carried out. Realistic warming was found to increase the rates of protease and urease activity during all tests; however, amidase activity was only measurable after the addition of labile carbon and even then showed no temperature sensitivity. This thesis also considered the effect of temperature change on the size and structure of the soil microbial community at these realistic soil temperatures. Both in the lab and the field changes in rates of soil processes (enzyme activity) as a result of temperature change are not accompanied by a change in either size or structure of the microbial community as measured by phospholipid fatty acid analysis, suggesting high levels of functional redundancy within the soil microbial community. The effects of organic matter input in the field were found to have only small effects on the rates of enzyme activity although this was more important during laboratory incubations. Organic matter quality was also important during lab incubations where lower quality organic matter provoked greater enzyme activity in accordance with q-theory; however, there was no evidence for greater temperature sensitivity of low quality organic matter. The size and structure of the microbial community, both in the field and in the lab, were not affected by either the rate of organic matter input (in the field) or they quality of organic matter (in the lab). The size of the microbial community, however, decreased over time in both situations, the ratio of bacteria to fungi in the soil seemed to increase over time also.

No description available.

No description available.

Grassland is an important part of the ecosystem in the Canadian prairies and its loss and fragmentation affect biodiversity, as well as water and carbon fluxes at local and regional levels. Over the years, native grasslands have been lost to agricultural activities, urban development and oil and gas exploration. This research reports on new methodologies developed for mapping the spatial extent of native grasslands to an unprecedented level of detail and assessing how the grasslands are fragmented. The test site is in the Newell County region of Alberta (NCRA). 72 Landsat and 34 SPOT images from 1985 to 2008 were considered for the analysis. With an airport runway used as a pseudo-invariant feature (PIF), relative radiometric correction was applied to 17 Landsat and 8 SPOT images that included the same airport runway. All the images were classified using the Support Vector Machine (SVM) classification algorithm into grassland, crop, water and road infrastructure classes. The classification results showed an average of 98.2 % overall accuracy for Landsat images and SPOT images. Spatial extents and their temporal change were estimated for all the land cover classes after classifying the images. Fragmentation statistics were obtained using FRAGSTATS 3.3 software that calculated land cover pattern metrics (patch, class and landscape). Based on the available satellite image data, it is found that in Newell County there is almost no significant change found in the grassland and road infrastructure land cover in over two decades. Also, the fragmentation results suggest that fragmentation of grassland was not due to the result of road infrastructure. / x, 105 leaves : ill., ; 29 cm

Major transitions between vertebrate and insect pollination systems have occurred many times during the angiosperm radiation and are associated with evolutionary modifications in floral traits. In the large ancestrally bird-pollinated African genus Protea (Proteaceae), an evolutionary shift from bird to insect pollination in the genus is suggested by the fruity diurnal scent of flowers in a recently evolved clade of grassland species. In this study, I confirm that four of these grassland Protea species have mixed mating systems and are indeed insect pollinated, and furthermore demonstrate the functional significance of their floral presentation and scent chemistry for attraction of pollinators, specifically cetoniine beetles. The study species, Protea caffra, Protea dracomontana, Protea simplex and Protea welwitschii, have colourful bowl-shaped inflorescences that produce copious amounts of pollen and dilute, xylose-rich nectar. Cetoniine beetles were found to be the most suitable pollinators due to their abundance, size, relatively pure Protea pollen loads, and their preference for the fruity scent and low growth form of these scented Protea species, as demonstrated by choice experiments in which inflorescences were offered at either end of a y-maze or at various heights above the ground, respectively. Bagging and hand pollinations revealed that these Protea species are self-compatible and capable of autonomous selfing. Self progeny of P. caffra were as vigorous as cross progeny in terms of germinability and survivorship to two months. Vertebrate-excluded and open-pollinated inflorescences yielded similar seed numbers for all species. Supplemental hand-pollinations, however, failed to increase seed set substantially, an indication of resource limitation. Outcrossing rates estimated using polymorphisms at eight allozyme loci in progeny from vertebrate-excluded and open-pollinated treatments of P. caffra were no different (t=0.59), indicating outcrossing by insects and an equal or insubstantial contribution from bird pollinators. The fruity-sweet scents of these species were more complex, with higher whole flower and mass-specific emission rates, than those in eight bird-pollinated congenerics. The overall floral scent is shown to be a blend of emissions from various plant parts, especially nectar. Electroantennography (EAG) revealed that the generalist pollinator Atrichelaphinis tigrina responds to a variety of volatile compounds found in fruity Protea scents. Field trapping confirmed that this cetoniine beetle is strongly attracted to ß-linalool (up to 60% of scent profile) and methyl benzoate. In conclusion, this study demonstrates the evolution of beetle pollination and mixed mating systems in a grassland clade of Protea. Volatile compounds that make up the unique (within Protea) fruity scent of the study species are shown to attract beetles, and the emission of large amounts of these compounds was probably a key step in the transition from bird to insect pollination in Protea. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.

The main aim of this study was to classify and map the threatened coastal grassland communities of the 3 257 ha Umtamvuna Nature Reserve (UNR) in KwaZulu-Natal (30°07'30" to 300 11'05"E; 30°55'00" to 31°04'30"S). Secondary aims were to relate past management and selected environmental variables to community composition and to develop guidelines for monitoring. Alpha diversity was measured using a Whittaker plot and revealed 119 species. A pilot study to test the efficiency of botanical techniques showed that a point based technique (nearest plant method in a 20 X 20 m plot) was efficient (52 minutes for recording 200 points), but recorded only 23% of the species. By increasing the number of points to six hundred, 34% of the species were recorded in 178 minutes; the same time was required to randomly place 30 quadrats (50 X 50 cm), which revealed 80% of the species. Tests for replicate similarity showed a high retrieval of internal association (PS = 86%), using abundant species only and 100 points per plot. The point based technique was thus efficient in detecting abundant species and was acceptable for producing a classification, especially in this case where a comprehensive species list already existed. Indirect gradient analysis (TWINSPAN) identified six grassland communities. An ordination using detrended correspondence analysis (DCA) contributed towards the community classification and grazing and fire frequency gradients were inferred from this ordination. Protea roupelliae communities were common but did not influence grass species composition . Canonical ordination revealed that, of the eight environmental variables measured, 'distance from the sea' strongly affected species distribution (r= 0.83). Cost effectiveness was considered in the development of a monitoring programme. Point based monitoring techniques favoured by sourveld researchers in KwaZulu-Natal were found to be inefficient, particularly for studies requiring the measurement of both species richness and community composition. Randomly located 100 X 100 cm quadrats, located in selected sites which represent previously identified communities, was more efficient. This study contributed towards a refinement of information on the grassland communities of KwaZulu-Natal and supported the use of point data for the classification of grasslands not previously studied. It also demonstrated that point based techniques were not suitable for meeting all grassland monitoring requirements. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 1995.

Stoats are introduced mammalian carnivores implicated in the decline of several of New Zealand�s endemic species. Most research into stoats in New Zealand has focused on beech forest habitat, especially in years of peak stoat abundance following heavy beech seedfall and peak cohorts of mice. In New Zealand, alpine grasslands occur above the altitudinal limit of beech forest (900-1000 m a.s.l.). Although previous research has shown stoats to be present there, little is known about the ecology of stoats in alpine grasslands. This research aimed to test whether alpine grasslands were a marginal habitat occupied by surplus stoats that had spilled over from beech forest populations, i.e. a sink habitat. The alternative is that alpine grasslands are a desirable habitat deliberately exploited by stoats. This question was answered using mark-recapture, radio-tracking, diet analysis and a food addition experiment. Another objective was to determine whether nest survival is higher in alpine grassland compared to beech forest and whether stoats are likely to be a frequent predator of ground nests in alpine grasslands relative to other introduced mammals that inhabit them. If nest survival is higher in alpine grassland then alpine grasslands may be a refuge from predation. However, if it is not then it is important for management to know the relative risk posed by stoats compared with other predators. An artificial nest experiment was used to answer these questions. This research was undertaken during two years of low to intermediate beech seedfall and therefore provided an opportunity to look at the ecology of stoats in a New Zealand National Park outside years of peak abundance. The principal study site for this research was the Borland Valley, Fiordland National Park. Compositional analysis showed that stoats in alpine grassland selected for it over adjacent beech forest. The range cores of these stoats were high up in alpine grassland away from the ecotone with beech forest. Stoats occurred at similar densities in alpine grasslands as they did in beech forest and observed survival was similar between the two habitats (with the exception of 2004 when it may have been higher in alpine grassland). The most frequent prey of stoats inhabiting beech forest were birds and mice. Although stoats in alpine grasslands also ate birds and mice their most frequent prey were ground weta and hare. Food addition appeared to cause diet switching but did not reduce the distances moved by stoats, suggesting that other factors may be more important in regulating their summer home range size in alpine grasslands. All of these factors lead to the conclusion that alpine grasslands in the Borland are not a marginal habitat for stoats, but may instead be a desirable one. Artificial nests had a higher probability of survival in alpine grassland compared to adjacent beech forest, but survival was too low to support the idea that alpine grasslands are a refuge. Stoats were the most frequent predator of artificial nests in both habitats, but 95 % confidence intervals overlapped the predation rate by possums, which was also high. These findings illustrate the need for a comprehensive landscape approach to stoat control in montane National Parks, for two reasons: 1) endemic biodiversity in alpine grasslands may be under threat from stoat predation, 2) alpine grasslands may act as a source for dispersing stoats that reinvade lowland stoat control areas. In the absence of heavy beech seedfall and peak mouse abundance, stoats occurred at densities of around 1 km⁻� in both habitats and there was recruitment into these populations. This raises the important question: What regulates the distribution and abundance of stoats in years of low beech seedfall and low mouse abundance? In these years birds, ground weta and hares may be as important as mice are in years of peak abundance following heavy beech seedfall.

Since European settlement, alpine and sub-alpine grasslands in Victoria have been grazed more intensively and wildfires have become more frequent. Alpine plants, however, have primarily evolved in the absence of fire and grazing, suggesting that they might be particularly sensitive to these factors. Little is known about the short-term physiological responses to defoliation of these predominantly resprouting plants, and this thesis investigates changes in the carbohydrate storage reserves of alpine plants during recovery from fire and/or grazing. (For complete abstract open document)