<p>Hedgehog related genes have been shown to play a major role in development in all deuterostomes. In C.elegans, such genes have been found where the similarity is restricted to the C-terminal domain. This work has focused on the hedgehog related C.elegans proteins called ground (grd), ground-like (grl), and wart (wrt) which appear to form a unique structural family.These proteins are cysteine rich and have conserved cysteine patterns which, together with thethought that they are secreted, are expected to be in disulfide form. Since the extracellular environment is very oxidizing and due to the conserved cysteine pattern, disulfide bonds are thought to play a big part in the folding and stabilization of these proteins. The stability of the protein and the formation of a disulfide bond are related through a thermodynamic cycle, which insures that the stabilization of the protein by the disulfide is reflected by the identical stabilization of the disulfide by the protein. Practically, there are numerous parameters that can be used to try to achieve the correct disulfide bonds and folding, when doing in vitro trials, some of which were used in this project. C.elegans proteins grd-5, grd-13, grl-24, wrt-3 and wrt-5 were studied in this project. All of the proteins were expressed and purified with success, with theexception of grl-24. All constructs formed inclusion bodies. Some refolding attempts were performed on grd-13 and wrt-3. The presence of a disulfide bond in refolded grd-13 was demonstrated using chemical fragmentation. In general, these attempts did not give correctly folded proteins but provide a foundation to continue experiments aimed at producing a native-like protein for structural and functional studies.</p>

Past weapons production activities have resulted in mass quantities of trans-uranic waste being buried in drums at several sites in the United States. In an effort to relocate these waste drums to more permanent storage sites, Fluor Hanford has begun characterizing their contents to ensure compliance with various shipping and storage requirements. Non-destructive analysis techniques are regularly employed, among them passive radiation detection using a Canberra Gamma-Energy-Analyzer germanium detector vault. Necessary strict legal tolerances require strong quality assurance. The detectors are frequently calibrated in the traditional method with check sources, but it would be advantageous to have an estimate of system minimum detectable activity (MDA). However, any estimate is complicated by the fact that sources are distributed stochastically in the waste drums. In this study, a method was developed to predict system detector efficiency for a variety of detector configurations and drum fill materials and calculate MDA based on these efficiencies. The various system designs were modeled in Monte Carlo N-Particle Code, version 4b, to determine photopeak detection efficiency. An external code written in C programming language was used to randomly assign between one and 20 sources to volumetric regions of the waste drum. Twenty simulations were performed for each design and drum fill material combination, each time redefining the stochastically distributed source. This provided a normally distributed spectrum of 20 efficiencies for each situation. From this, mean and lower 95% confidence limit efficiencies were used to calculate MDA. The patterns among the results were then compared with values predicted by the MDA formula. Finally, an examination was made of the impact on the MDA of the system's true design in the case of single or multiple detector failure. The results indicate that this method of estimating minimum detectable activity, although costly in computing time, provides results consistent with intuitive and calculated expectations. Future work would allow easy calibration of the model to measured efficiency results. Used in coordination with physical experiments, this method may eventually prove useful in benchmarking system performance and accurately ensuring reliable waste drum characterizations. / Graduation date: 2003

Graduation date: 1997

Codons are simple primitives for describing plane curves. They thus are primarily image-based descriptors. Yet they have the power to capture important information about the 3-D world, such as making part boundaries explicit. The codon description is highly redundant (useful for error-correction). This redundancy can be viewed as a constraint on the number of possible codon strings. For smooth closed strings that represent the bounding contour (silhouette) of many smooth 3D objects, the constraints are so strong that sequences containing 6 elements yield only 33 generic shapes as compared with a possible number of 15, 625 combinations.

Gait biomechanics of forty male subjects was evaluated at normal and fast walking speeds. The forty subjects composed four groups based on their body mass index, with ten subjects in each of the groups: underweight, normal weight, overweight and obese. To our knowledge this is the first comprehensive 3-dimensional kinetic and kinematic gait analysis of all four groups based on body mass index. The obese subjects walked with significantly slower gait speed by taking shorter steps and strides, while having significantly higher step widths and longer gait cycle times than the other subjects. The obese subjects spent significantly less time in single support and more time in double support than their non-obese counterparts. These adjustments in temporal characteristics for the obese participants may be as a result of the gait compensation for the additional body weight in order to give them the most efficient, stable and balanced walking ability. Body mass index affected significantly the forces and moments at the ankle, knee and hip in the medial-lateral plane while speed effects were more prominent in the sagittal and transverse planes. These results suggest that an increase in the body weight would affect the gait stability while increasing the speed will affect the gait progression. Contrary to most researchers beliefs that an increase of the body weight would increase the forces and moments of the knee in all three planes, this study was able to prove that the actual forces and moments in the medial-lateral plane for the knee joint decrease while the ones in the sagittal plane increase. On the other hand, the hip joint in the medial-lateral plane displays the highest forces and moment for the obese subjects. These results are indicative of a gait compensation related to increasing body weight in the medial-lateral compartment of the lower extremity joints. Recommendations for further studies and follow up experiments are enclosed.

Thesis (M.S.)--West Virginia University, 2006. / Title from document title page. Document formatted into pages; contains x, 100 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 83-86).

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Lick Creek Park is a 515 acre nature park that was acquired in 1987 by the City of College Station, Texas. The site has a variety of indigenous plant and animal species and is an important natural resource for citizens of the region. There is a long-term commitment to inventory this natural park to monitor the changes as our urban community expands to surround the park. There are 989 species of spiders currently recorded from Texas and 332 of them are known to occur in Brazos County. My focus was on improving the ground spider inventory at Lick Creek Park. Spider collections were made using 18 regularly-sampled pitfall traps distributed evenly among three habitats. Spiders from 24 families, 66 genera, and 111 species were identified from 918 specimens, including 627 immature and 291 adult spiders, captured in pitfall traps from April 2005-April 2006. Of the 111 species found, 45 were represented by one specimen only and 20 were represented by two specimens. Rarefaction analyses indicated that the majority of spider species were readily detectable using pitfall traps and inventoried during this study (111 found and 168 estimated to be present). Simpson’s Diversity measure bootstrap estimates determined species diversity overall to be very diverse (0.966), as did a Shannon Weiner Diversity bootstrap estimate (5.483). Also, Simpson’s measure of species evenness (0.264) indicated a low species evenness. Those species found in only one habitat comprised 50% of the total species, and their densities ranged from 1-5 individuals. Those species found in just two habitats comprised 25% of the total species, and their densities ranged from 2-21 individuals. Species found in all three habitats comprised the remaining 25%, and their densities ranged from 4-53 individuals found. Most species occurred at low densities in this study and this often precluded conducting more detailed analyses. Additional sampling is expected to, first, detect known species occurring in previously unrecorded habitats and, second, to detect species not previously found in the park. This inventory of spiders at Lick Creek will provide a basis for further studies on biodiversity and the assessment of human impact on the environment.

Tunable diode laser absorption spectroscopy using an external cavity diode laser operating in the infra-red has been developed to monitor CO2 in the freestream of the Longshot hypervelocity facility at the Von Karman Institute for Fluid Dynamics. The Longshot facility offers a unique European facility for ground testing and numerical validation applications, however, some of the traditional data rebuilding aspects are in question. A non-intrusive absorption sensor could significantly aid in improving the knowledge of freestream static values thereby improving the models used in data rebuilding and numerical simulation. The design of such a sensor also expands the spectroscopic capabilities of the Von Karman Institute. The absorption sensor is designed around the single P12 (00001)-(30013) rovibrational transition near 1.6µm (6218.09cm-1 specifically) which yields relatively weak direct absorption levels at about 3.5% per meter for typical Longshot freestream conditions. However, when handled carefully, adequate signal-to-noise can be acquired to exploit significant flow information. By being able to operate in this range, total sensor cost can be easily an a factor of two or more cheaper than sensors designed for the deeper infrared. All sensor elements were mounted to a compact portable optics bench utilizing single-mode optical fibers to allow for quick installation at different facilities by eliminating tedious optical realigning. Scans at 600Hz were performed over 20ms of the 40ms test time to extract core static temperature, pressure and velocity. These results are compared with the current state of the Longshot data rebuild method. The non-uniform flow properties of the shear layer and test cabin rested gas accumulation was of an initial concern. The temperature and density gradients along with significant radial velocity components could result in DLAS temperature, pressure and velocity that are significantly different than that of the target freestream inviscid core values. Fortunately, with the proper selection of the P12 rotational number, this effect could be more or less ignored as the higher temperature and lower density gas of this region is relatively transparent. Ultimately, acquired temperature and density were moderately accurate when compared to Longshot rebuilt results owing primarily to the baseline extraction which poses issues for such low absorption signals. However, the extracted velocity data are quite accurate. This is a definite puls for the sensor as the freestream enthalpy of cold hypersonic facilities is dictated primarily by the kinetic energy contribution. Being able to compare velocity gives insight to the level of vibration non-equilibrium in the flow. The velocity of the DLAS and the Longshot rebuild are quite close. This adds more weight to the argument that vibrational excitation is very low (if present at all) in the free stream and that the van de derWaals equation of state usage and constant specific heat assumption might be an adequate model for the data rebuild after all.

Railway track and ground vibrations are considered, with anemphasis on methods of mitigation ("countermeasures"), forapplication to wayside disturbance problems. Original field measurements from two sites in Sweden, aswell as borrowed measurements from Norway, provide vibrationresults at many points on the track, on and underneath theground surface, for a variety of trains, both with and withoutcountermeasures in-place. Infinite periodic system theory is the basis of track-onlyand track-ground interaction models presented. The repeatingelement includes the sleeper, pad-fastener, rail, and either alocally-reacting ballast or a continuous ballast-soilwaveguide. The track-only model is even refined for nonlinearand high-frequency cases. The models are suitable for studyingcountermeasures in the track, or in the foundation(soil-stabilization). This latter countermeasure is shown to beeffective at low frequencies (of geotechnical interest), butsometimes counterproductive at audible frequencies (disturbanceproblems). An analytical model for hard seismic screens is alsopresented, to complement the treatment of ground vibrationcountermeasures; this is based on physical approximations whichare favored by "high" (i.e, audible)frequencies and softsoils. Notably, experimentally observed resonant behavior isexplained. <b>Keywords:</b>ground vibration, vibration screen, trackvibration, railway vibration