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Geophysical investigation of the subsurface through seismic refraction and reflection methods provides an efficient and non-invasive means towards addressing geologic problems across multiple scales. Both seismic techniques, in an active-source exploration setting, involve inducing acoustic waves into the subsurface and measuring their propagation velocities and amplitudes. These measurements have physically-based relationships with the properties of the underlying strata, thus allowing changes in the seismic measurements to be interpreted with respect to changes in the subsurface geology. Two applications of the seismic method are presented in this dissertation: (1) shallow seismic refraction acquisition and processing applied to the near-surface investigations of soil and regolith, which constitute the Critical Zone (CZ), beneath the upland hillslopes of the Valles Caldera, New Mexico; (2) interpretation of 2-D and 3-D marine seismic reflection data that image the upper 10-km of the crust beneath the Southern Guinea Plateau, offshore Guinea, West Africa. In both cases, the seismic data provide necessary constraints for the generation of accurate subsurface models that permit further geophysical modeling. The near-surface results, presented in Appendix A, provided a rich dataset of weathered thicknesses across hillslopes that supported an investigation of potential relationships between CZ geologic architecture and topographic attributes. Quantified relationships suggest that calibrated predictions based on the topography can provide first-order estimates of regolith thickness across upland landscapes. These results add to the ongoing CZ-science endeavor to understand proposed links between subsurface weathering processes and their surface expressions. In Appendix B, interpretations of high-resolution 3-D seismic data have illuminated deformational structures associated with Mesozoic rifting of the Southern Guinea Plateau. The interpretations were expanded onto regional 2-D seismic profiles, permitting a regional synthesis of the southern margin’s structural evolution. Additional tectonic subsidence and forward-gravity modeling highlight the influence of Jurassic rifting on the Southern Guinea Plateau prior to Early-Cretaceous rifting and separation, as well as crustal thickness estimates from the continental shelf out towards oceanic crust. Lastly, the Guinea-Demerara conjugate plateaus, and their associated deformations, were restored to 100 Ma, revealing an apparent upper-crustal asymmetry between the two margins. Appendix C presents two seismic-exploration methodologies based on 3-D seismic reflection data: (1) the calculation and interpretation of two co-rendered volumetric seismic attributes – most-positive curvature and semblance; (2) numerically modeling the tectonic subsidence of an entire 3-D seismic survey. Both techniques are used to address the inherent difficulty in interpreting the extent to which Jurassic rifting affected the Southern Guinea Plateau. Furthermore, the numerical model of subsidence provides a new exploration technique towards qualitatively and quantitatively assisting in the assessment of potential hydrocarbon-bearing basins.

Despite social scientists' interest in globalization, mobility, the effects of colonialism, and the intercultural situations that result, little attention has been devoted to expatriates as a contemporary transnational group. This thesis is an enquiry into the ways eight individuals define themselves as expatriates, through their oral narratives of life in Papua New Guinea. The paper focuses on expatriates' characterizations of themselves in terms of: their communities; their relationships with locals; their status as foreigners in post-colonial Papua New Guinea; arid their experiences of mobility. Set against social scientific notions of expatriates and contemporary ideas of mobility and its relation to identity, expatriates' personal narratives indicate that scholarly depictions are too simplistic to access contemporary expatriates or the complex situations in which they live. / Arts, Faculty of / Anthropology, Department of / Graduate

Calcium transport across the placenta is asymmetrical and is believed to be an active transport. An essential step in such a transport is translocation of the ion across a single plasma membrane. The objective of this thesis was to study the Ca2+ -related properties of the placental plasma membranes and to gain some knowledge of their role in Ca2+ -transport. Three Ca2+ -related properties were studied: 1. Ca2+ -binding to the placental plasma membranes; 2. The membrane bound enzyme Ca -ATPase; and 3. Ca2+ -uptake by the placental plasma membrane vesicles. Ca2+ -binding properties of the membrane preparation were studied by the use of a new method, the flow dialysis system. Two types of sites for Ca were found: 1) high affinity, low capacity sites, and 2) low affinity, high capacity sites. The high affinity sites had 10-fold higher affinity for Ca2+ than for Mg2+ . A calcium-stimulated, membrane-bound enzyme, namely Ca2+ -ATPase, was located in the placental plasma membranes. This enzyme is distinct from the Na+, K+-ATPase and alkaline phosphatase. The enzyme can be activated by Mg2+ but with lower efficiency. Both Ca2+ and Mg2+ activate the enzyme at the same site. A formula was derived, enabling one to predict very precisely the velocity of the enzyme incubated under any combination of Ca2+ and Mg2+ ; this relationship is presented in a three dimensional model. The formula can be used for other enzymes or other substrates, as was demonstrated with ATP and ADP. The placental plasma membrane vesicles are capable of accumulating Ca2+ . Ca2+ -uptake was defined as the amount of Ca2+ which is not available for rapid exchange and cannot be displaced by a high concentration of competitor in the presence of ATP. This definition is different from and more accurate than the one which is widely used and cited in the literature. An intravesicular Ca2+ concentration of 190 mM was recorded, which was 24-fold higher than the external Ca2+ concentration (8 mM). Ca2+ -uptake was dependent on ATP hydrolysis by the placental Ca2+ -ATPase. This process was independent of Mg2+ . It is suggested that while the substrate for Ca2+ -ATPase is Ca-ATP, the substrate for Ca2+ -uptake is Ca2+. The overall Ca2+ -related properties of the placental plasma membranes are independent of Mg and the entire process from binding to membrane through activation of the enzyme and finally Ca2+ -uptake is dependent on Ca2+ alone. This situation is unique to the placental plasma membranes. It is tempting to speculate that the link between the maternal and the fetal circulation is achieved by forming vesicles loaded with Ca2+ on the maternal side and unloading them through fusion with the basal plasma membrane on the fetal side. The Ca2+ -related properties of placental plasma membranes described in this thesis, provide many answers regarding the first step in the asymmetrical transplacental Ca2+ -transport. Further investigation is required before a full understanding of the entire process is achieved. / Medicine, Faculty of / Cellular and Physiological Sciences, Department of / Graduate

This thesis examines the theme of order and entropy in the society, religion, and life of the Telefolmin people of Papua New Guinea, with an emphasis on the interpretation of secret rites and myths of the men's cult. Based on research in Telefolmin in 1974-5, the thesis draws upon the perspectives of Turner, Wagner, and Burridge. In the Telefol view order is a contingent construction which men maintain in the face of the world's drift toward entropy, corresponding to the concept of 'biniman' the process of dissipation and decay, 'becoming nothing'. The struggle against entropy informs several sectors of Telefol life, ranging from marriage practices to food tabus. A major strategy involves the segregation of antithetical acts and states, summarized in the polarization of nurturing and killing, which forms the major axis of the cult division between Taro (gardening, etc.) and Arrow (hunting, warfare, etc.). The anchoring point of the Telefol world is the men's cult house, which youths enter through a series of initiations. The rites are examined in detail, accompanied by an account of secret myths revealed in initiation. Analysis of the logic of secrecy shows that the multi-layered revelatory process illuminates principles of Telefol order while at the same time negating them. Thus the initiatory process highlights the dissonances of Telefol culture, calling "first principles" into question. This extends even to the notion that secret knowledge is capable of making reality transparent, a point underscored by the transcendental role of Magalim, a spirit embodying the notion of entropy. The thesis concludes by suggesting that Telefol religion comments on the possibilities of knowledge, men's hopes, the meaning of human action, and man's nature. Far from escaping life's ambiguities, men encounter them forcefully in Telefol religion. This implies that the anthropology of religion should be prepared to do no less. / Arts, Faculty of / Anthropology, Department of / Graduate

The excitation-contraction-relaxation cycle of the guinea pig ileum longitudinal smooth muscle was studied in muscles contracted by a muscarinic agent, cis-2-methyl-4-dimethylaminomethyl-1,3-dioxolane methiodide (CD) and by 60 mM KC1. Aspects of the cycle were investigated by analyzing the active transport enzyme activities in the sarcolemma, the tissue Ca depots which could release Ca for contraction and the sensitivity of the contractile responses to extracellular ion changes. Essentially net changes of intracellular Ca, Mg, Na and K content during contractions were measured by a modified 'La method'. The tissues were washed for 30 min in 160 mM Tris-HCl solution (pH 7.4) containing 10 mM LaCl₃ at 4°C in order to seal the intracellular ions in the cell and displace extracellular ions. A method to loosen the 'intercellular cementing' substance by reducing the tissue Ca and Mg was developed as an adjunct to the preparation of a sarcolemmal enriched microsomal fraction. The method reduced the tenacity of the tissue and made the tissue easy to disrupt by a mild homogenizing procedure. The method also appeared to aid the extraction of contractile proteins. The microsomal fraction was not detectably contaminated by mitochondria and was enriched with vesicles of sarcolemma, probably originating from the muscle caveolae. The sarcolemma enriched microsomal fraction had a Ca-ATPase activity that was progressively stimulated by 10⁻⁷ to 2.4 x 10⁻⁴ M free Ca²⁺ , did not require Mg and was inhibited by La. The microsomal Ca-ATPase activity was not due to contamination by actomyosin. The actomyosin Ca-r-ATPase in the soluble fraction had a higher affinity than the microsomal Ca-ATPase for Ca and for La. The microsomal Ca-ATPase activity was postulated to be associated with an active Ca pump thought to he located in the cayeolae. The microsomal fraction had a Mg-dependent ATPase that could Be stimulated by Na, but K and ouabain had very little additional effect. The addition of an activating factor in the soluble fraction conferred some K and ouabain sensitivity to the Mg-dependent Na-ATPase, which indicated that a Na,K-ATPase was present in this tissue. Low doses of ouabain contracted the longitudinal ileum but the responses were not antagonized by raising the external K concentration five fold, as would be expected if ouabain acted by inhibiting the Na,K-ATPase. However, the ouabain response was rapidly lost when extracellular Ca was removed from the medium and the decline of the response followed the same time course as the loss of extracellular Ca. The peak of the ouabain contraction coincided with significant increases of intracellular Ca and Na, but K loss was not apparent until relaxation ensued. The results suggested that ouabain has an early direct effect on membrane permeability before it inhibited the Na,K-ATPase. CD (2 x 10⁻⁷ M) and 60 mM KCl induced phasic and tonic contractions of the longitudinal muscle of the ileum. The phasic contraction declined from 100% to 7% over 10 min when Ca was omitted from the physiological medium. This decline followed the time course of the loss of extracellular Ca. This, together with the fact that low concentrations of LaCl₃ inhibited the phasic component, indicated that Ca bound to the outer aspect of the cell was responsible for the phasic component. The tonic component was lost more rapidly than the phasic component when the Ca was removed from the Tyrode's solution. The tonic component seemed activated by free Ca mobilized from the extracellular space. The extracellular origin of the Ca for contraction was consistent with the observed small net gain of intracellular Ca that occurred during the phasic and tonic contractions. The minimal volume of the sarcoplasmic reticulum and the abundance of caveolae was also consistent with the high sensitivity of the tissue to extracellular Ca concentrations. The intracellular Ca gained during contraction wa,s extruded within 30 sec after the CD or 60 mM KCl were washed out of the tissue bath, Following washout of CD, the muscle was quiescent for the 20 to 30 min 'equilibration' phase. Spontaneous activity was absent during this phase and tension was below baseline. After a maximal CD contraction, a second response to CD or to 60 mM KCl induced during the 'equilibration' phase had an altered or desensitized biphasic appearance. Responses of the muscle to CD for 10 min were accompanied by a cytoplasmic loss of K. After washout of CD, the K was regained slowly over 20 to 30 min. Stimulation of the tissue by 60 mM KCl did not cause a loss of K from the muscle nor did it cause desensitization of the muscle. Higher extracellular K concentrations decreased the time required after CD contractions for the return of spontaneous activity and prevented muscle desensitization to repeated doses of CD, probably by accelerating the return of intracellular K levels to normal. It was proposed that during contraction, elevated intracellular Ca activated K channels, thereby increasing K permeability and causing the 'after-hyperpolarization' and subsequent desensitization which follows muscarinic induced contractions. / Pharmaceutical Sciences, Faculty of / Graduate

It is the aim of this work to study the role, if any, of C'4 in the immune response against Staphylococcus aureus infections.

This thesis is a study of the effect of lipid injections on complement titers of guinea pigs.

Please read the abstract in the section 00front of this document / Dissertation (MSc Agric (Animal Nutrition))--University of Pretoria, 2006. / Animal and Wildlife Sciences / unrestricted

Unconstitutional changes, especially coups d’états, have undoubtedly eroded peace and security in many parts of the African continent. These occurrences have also stunted the development of democracy in some African states. The African Union (AU), supported by sub-regional bodies, addresses this problem by imposing sanctions on the regimes that acquire power through coups with the aim of restoring political order. However, this sanctions policy has produced mixed results. In some cases, these sanctions managed to succeed in achieving their objectives (Guinea) while in other instances sanctions failed to achieve their objectives (Madagascar). It is therefore imperative to inquire into the circumstances and assess the conditions under which the AU sanctions policies failed and succeeded in restoring political order to states that experience coups d’états. / Political Sciences / M.A. (International Politics)