Hydrilla (Hydrilla verticillata) is an invasive aquatic plant that grows quickly across shallow freshwater habitats. It is a problem for recreational users of lakes and landowners. Grass carp (Ctenopharynogodon idella) is an effective biological control agent that preferentially consumes and can control the spread of hydrilla. However, grass carp also will consume other vegetation, which influences aquatic communities via direct and indirect interactions that can change food and habitat availability and use by various species. Aquatic plants influence habitat and types of prey used by sunfish (Centrarchidae), which must also avoid their own predators. Prey use among sunfish species depends on density and taxonomic identity of both prey and vegetation. This was a one-year analysis of stomach contents from three common species of invertivorous sunfish: bluegill (Lepomis macrochirus), longear sunfish (Lepomis megalotis), and redear sunfish (Lepomis microlophus). Thirteen sampling stations were randomly selected using ArcGIS software. Percentage of water surface covered by vegetation was recorded at each station. A five-minute electrofishing sample was performed within the littoral zone early morning in late September. The stomach contents of all targeted sunfish (N=489) showed high percentages of diet overlap pre- (0.77-0.92) and post- (0.83-0.88) introduction of grass carp. Multivariate analysis showed total explained variation (15.5%) in sunfish diet composition was (P < 0.05) correlated significantly with sunfish species (6.67%), percent surface vegetation coverage (3.97%), and sampling periods pre- versus post-introduction of grass carp (2.13%). Prey-specific abundance showed that all sunfishes displayed a generalized feeding strategy in both sampling periods. Diets of each sunfish species showed differences in abundance (by volume) and occurrence (among individual fish) of prey items between sampling periods. Levin?s standardized index of diet breadth for all sunfish species decreased from pre- (0.12) to post-introduction (0.05). Results imply that vegetation control by grass carp influenced the diets and feeding strategies of three cohabitating sunfish species. These findings may help fisheries biologists to plan future management actions that influence assemblages of aquatic plants and macroinvertebrates, herbivorous fish, invertivorous prey-fish, and piscivorous game fish, to promote a healthy and balanced ecosystem for Lake Conroe stakeholders.

Historically, terrestrial food web research has focused on describing the structure of aboveground grazing webs, and determining how interactions among plants, herbivores and higher trophic levels influence primary productivity. Detrital food webs however, play a significant role in regulation of ecosystem dynamics through direct impacts on decomposition. Unraveling the complex nature of detrital food web structure is critical to developing a better understanding of ecosystem function. Therefore the primary objective of this research was to describe the structure of the leaf-litter food web in a temperate deciduous forest, with emphasis on interactions between a community of generalist predators, the forest-floor spiders, and arthropod prey. Elucidating occurrence of trophic interactions in the forest-floor food web was a formidable task due to the high diversity, small body sizes and cryptic habits of many litter-dwelling arthropods. Analysis of natural variation in consumer stable isotope ratios (δ13C and δ15N) formed the crux of this research because it simultaneously permitted quantification of the trophic positions of litterdwelling arthropods and identification of spider resources, including prey subsidies from the grazing web. A monoclonal antibody-based ELISA was employed to analyze the gut contents of spiders to quantify predation on a major arthropod taxon, the forest-floor flies. Surveys of spider distributions and prey availability in the litter layer also provided fundamental knowledge of community structure. Stable isotope analyses suggested that most spiders exhibited strong trophic connections to the detrital web, but weak links to herbivorous prey. Several lines of evidence supported a strong trophic link between large, litterdwelling collembolans (Tomoceridae) and cursorial spiders, including correlation between spider and tomocerid densities on the forest-floor, similarities in spider and tomocerid carbon signatures, and nitrogen enrichment of tomocerids relative to other prey types. Conversely, this research provided conflicting evidence regarding spider consumption of flies. Gut content assays indicated consistent predation on flies by cursorial spiders, while stable isotope models suggested that flies are likely of little importance in the spiders’ diets. This project yielded valuable insights into the role of spiders in the forest-floor food web and the potential importance of species-specific variation in prey consumption for detrital food web dynamics.

According to optimal foraging theory, generalist predators, such as spiders, are thought to feed indiscriminately on prey according to its availability, especially when food is scarce. In contrast, generalists can display selective feeding decisions under regimes of high prey abundance, but few studies have tracked changes in prey choice on a seasonal basis under open field conditions. Additionally, adaptations to surviving winter have been largely ignored in the research of foraging behavior. To elucidate this, I monitored prey availability and collected common forest-dwelling wolf spiders for molecular gut-content analysis, in parallel for 18 months, to assess the temporal changes occurring in spider preferences of common leaf litter prey. In addition, to determine if any physiological improvements to resisting low temperature mortality were affecting spider foraging, I also collected spiders monthly to track changes in spider supercooling points. The results revealed that spiders do exhibit selective feeding throughout the year, and appear to do so in a way that diversifies their diets. Also, despite low litter temperatures putting them in severe freezing risk, cold tolerance in these spiders remained unchanged throughout the winter, which suggests opportunity for growth during this uncompetitive period is paramount to accumulating survivorship-increasing, but also mobility-decreasing, cryoprotectants.

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