京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第18168号 / 医博第3888号 / 新制||医||1003(附属図書館) / 31026 / 京都大学大学院医学研究科医学専攻 / (主査)教授 伊藤 壽一, 教授 大森 治紀, 教授 影山 龍一郎 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

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The objective of this study was to evaluate the relationship between hair coat shedding ability, apparent forage digestibility, and mineral status in Angus cattle. Fecal samples were collected on fall calving Angus cows for one week in March, May, and July from 2019-2022. Liver and plasma samples were collected in March and July each year. Fecal samples were analyzed for DM, ash, NDF, and ADF while liver and plasma were analyzed for mineral concentration. Data were analyzed using MIXED procedure of SAS with significance declared at P0.05. Interactions were detected for DM, NDF, ADF, and liver Fe concentration. The model included MFS, month, and MFS by month interaction for each response variable. Interpretation of results showed no clear trends or associations between hair coat shedding ability, apparent forage digestibility, and mineral status, but further investigation is needed to understand cow performance due to relationships between genes and environment.

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This paper explores white American masculinity within the “ginger” phenomenon. To guide this study, I asked: How is racism conceptualized and understood within popular culture, as seen through discussions of whether or not gingerism constitutes racism? How do commenters respond or interact when their understandings of racism or explanations for gingerism are challenged by other commenters? And finally, what does the creation of and prejudice against/making fun of a “hyperwhite” masculine identity at this social/historical moment suggest about the current stability of the dominant white masculine identity? Through discourse analysis of online comments, I explored discussions of race, gender, and gingerism. The analysis covered 6,413 comments on 102 articles. I found that within discussions of race and gingerism, readers made use of varying definitions of race and racism. Different definitions led to conflations of racism, oppression, and bullying. Simplified and individualized definitions of race and racism also led to arguments that supported frameworks of reverse racism and post-racism. So-called discrimination against redheaded men was overall considered to be more serious than for women. These arguments were bound up in questions of the specificity of cultural contexts, and ethnic and national identities, particularly with regard to Irish and Scottish immigrant heritage in the United States and United Kingdom. Future work should continue to untangle ideas of race and physical appearance and ask how whiteness is understood and works within this context.

The hair cell, a specialized cell in the inner ear, is responsible for hearing and balance. The hair cell is an exquisite sensor that captures mechanical stimuli and generates neurosensory signals. A theory called gating theory has been developed and widely used to analyze the experimental data of hair cell transduction. Despite increasing knowledge about molecular structures of hair cells, the mechanical model in the gating theory remained simple. Efforts to make the most of the recent findings regarding the hair cell structures led to the development of hair cell finite element (FE) model (Cotton & Grant, 2000, 2004a, b). I have extended this approach by adding channel kinetics and structural dynamics to the FE structural model of the hair cell. I have expanded the previous static and passive model to a dynamic and active model. It is the most detailed hair cell structural model and includes up-to-date knowledge of the hair cell structure such as the stereocilia and various extracellular links. In order to observe the dynamic response of hair bundles in the endolymph fluid, I have included fluid drag in the model. Link nonlinearity has been added to reflect recent observations (Tsuprun 2003). The lateral links stiffen as they stretch and prevent contact between stereocilia when they compress. In addition to these structural features, I added channel kinetics such as the fast adaptation. In my study, the Ca²⁺ diffusion kinetics plays a key role in the hair cell adaptations. The Ca²⁺ association rate to the fast adaptation modulator is postulated to govern the fast adaptation. I assumed that two factors--the tip link tension and the Ca²⁺ concentration at the tip of stereocilia govern the hair cell mechanoelectric transduction. My dissertation comprises three parts--structure, dynamics and mechanotransduction of hair cells. First, the mechanical properties of hair bundle were sought by comparing my FE model with other experiments. The quantified Young's modulus of stereocilia and the stiffness of tip link agree well with other recent estimates. The stiffness of other structural elements (upper lateral and shaft links) was newly estimated through this effort. Second, I established equations of motion for the hair bundle in the fluid. Two possible loading conditions to the hair bundle were simulated. Two different hair bundles were subjected to a point load and a load induced by fluid flow. The results showed that some vestibular hair cells' transduction might be dominated by the fluid-induced force. Finally, I observed the hair cell transduction in various stimulus conditions. The results showed that the hair cell's sensitivity highly depends on the stimulus method. The fluid-jet stimulus activated fewer channels than the glass fiber and made the hair cell less sensitive. A faster stimulus opened more channels and made the hair cell more sensitive. The resting tension in the tip link, which is believed to be controlled by the Ca²⁺ concentration, also affected the hair cell sensitivity. A higher resting tension, equivalent to a lower Ca²⁺ concentration, tended to make the hair cell more sensitive. In conclusion, I developed a new tool to study the hair cell mechanoelectric transduction. My hair cell computational model enables us (1) to study how the hair cells' morphological variations are related to their function; (2) to investigate the hair cell mechanoelectric transduction at the single channel level, in silico, as opposed to the statistical approach; (3) to test the response of hair cells under in situ force boundary conditions. / Ph. D.

Silvopastoral management has potential to diversify and increase the output from livestock production systems. Silvopasture production offers solutions to many management issues associated with grazing systems in the Appalachian region. Several tree species have been proposed for silvopastures in humid temperate regions, but little data comparing animal performance from systems with different deciduous tree species are available. Forage and animal performance was compared from open systems (i.e., no trees) with that from silvopastures containing 13-yr-old honeylocust (Gleditsia triacanthos) or black walnut (Juglans nigra) trees. Cool-season grass-legume pastures were rotationally stocked with hair sheep crosses from mid-June through September. The objective of this project was to determine carcass characteristics and meat quality of hair sheep crosses grazing honey locust or black walnut silvopastures in comparison with traditional pasture systems. Forage production varied by year with black walnut having lower production, especially in the 2009 season with black walnut producing less forage compared to open pastures and honeylocust silvopastures (P=0.0008). Only small differences in forage nutritive value were observed. Total gains and average daily gains (ADG) did not differ by treatment in 2008, but during the 2009 season black walnut silvopastures produced half the total gains (P=0.0427) and ADG (P=0.0513) of open pastures and honeylocust silvopastures. Carcass characteristics evaluated did not vary among treatments except hot carcass weight with black walnut having lower weights (P=.0045). Meat quality characteristics did not vary among treatments (P>0.1). Shear force and fat content showed great differences and varied by year (P<0.05). Overall, carcass and meat quality was similar for all treatments showing great promise for silvopastures. / Master of Science

The vestibular system of vertebrates consists of the utricle, saccule, and the semicircular canals. Head movement causes deformation of hair cell bundles in these organs, which translate this mechanical stimulus into an electrical response sent to the nervous system. This study consisted of two sections, both utilizing a Fortran-based finite element program to study hair cell bundle response. In the first part, the effects of variations in geometry and material properties on bundle mechanical response were studied. Six real cells from the red eared slider turtle utricle were modeled and their response to a gradually increased point load was analyzed. Bundle stiffness and tip link tension distributions were the primary data examined. The cells fell into two groups based on stiffness. All cells exhibited an increase in stiffness as the applied load was increased, but cells in the stiffer group showed a greater increase. Tip link tensions in the compliant group were approximately 3 times as high as those in the stiffer group. Cells in the stiffer group were larger, with more cilia, and also had a higher stereocilia/kinocilium height ratio than the cells in the other group. The stereocilia/kinocilium height ratio was the most important geometric factor in influencing bundle stiffness. Modeling a bundle as just its middle row of stereocilia resulted in some decrease in stiffness, but more significantly, a stiffness that was virtually constant as applied load increased. Tip link tension distributions showed serial behavior in the core rows of stereocilia and parallel behavior in the outer rows; this trend intensified if the tip link elastic modulus was increased. It was demonstrated that full three-dimensional modeling of bundles is critical for obtaining complete and accurate results. In the second part of the study, tip link ion gates were modeled. Sufficient tension in a tip link caused that link's ion gate to open, increasing the length of the link and causing its tension to decrease or the link to go slack. The two parameters that were varied were tip link elastic modulus and tip link gating distance d (change in length of the link). Bundle stiffness drops of up to 25% were obtained, but only when tip links went slack after gate opening; tip link slackening was dependent on tip link gating distance. Higher tip link modulus resulted in higher stiffness drops. Variable tip link modulus and tip link pre-tensioning were modeled. Variable tip link modulus resulted in increased bundle stiffness, especially under high applied loads, and in some cases, resulted in greater bundle stiffness drops when ion gates opened. Tip link pre-tensioning had no noticeable effect on bundle response. No evidence against inclusion of pre-tensioning or variable tip link elastic modulus was found. / Master of Science