Regrowth and yield of 23 Middle Eastern (ME) cultivars and the elite cultivar Lew were evaluated for twelve regrowth harvests in pure stands and 50:50 mixtures with Lew in 1988 at Tucson, Arizona. Stem elongation rate and crown shoot development were measured on selected ME cultivars and Lew in the spring of 1988. No significant differences in yield were observed between mixtures and monocultures. Few significant differences were observed over all yields among ME cultivars and Lew. Cultivars from Saudi Arabia and Egypt produced more forage than Lew in spring harvests. These cultivars developed crown shoots precociously and had high stem elongation rates during the first week of regrowth. Most ME cultivars had higher leaf area and leaf:stem ratio than did Lew. These findings suggest that ME cultivars may be useful in improving forage yield during the winter and spring and forage quality in elite nondormant cultivars.

Germination strategies and early seedling development of selected Medicago and Hedysarum species were evaluated to identify plants with high potential for range rehabilitation in central Tunisia. Temperature (5°C to 25°C) and water stress (0 MPa to -0.8 MPa) affected the germination percentage of all Medicago and Hedysarum species. Water stress had a greater effect on percent germination than did temperature, however, the nature of the effect of water potential depended on temperature. Australian medics were more depressed by temperature extremes and low water potential than were Tunisian accessions. Optimum germination of Medicago polvmorpha var. Circle Valley and Medicago trucatula var. Jemalong was at 15 °C and 0 MPa. Germination was highly reduced at higher temperatures and water stress. Tunisian Medicago truncatula germinated better at lower temperatures (5 °C to 15 °C). Hedysarum carnosum germinated more completely at high temperatures. (15°C to 20°C). Medicago laciniata germinated well across a wide range of temperature but germination decreased as water stress increased. Rapid rates of root elongation are beneficial to plants in semiarid environments. High temperature accelerated rates of root elongation and low temperature retarded the rates. The degree of retardation varied with the species and the temperature range. Maximum root elongation occurred at 15°C and minimum root elongation occurred at 5°C. Medicago laciniata had the fastest root elongation rate at 5°C and 15°C . Medicago truncatula was equal to Medicago laciniata at 5°C. Hedysarum carnosum had rapid root elongation at 10°C and 15°C compared to 5°C. A Tunisian accession Medicago polymorpha had the slowest root elongation at all temperatures. A quantitative growth analysis was used to assess the effect of environmental conditions on the species performance over a period of 49 days. Mean relative growth rate (mRGR) varied among species. This variation suggested size hierarchies in relative performance among species. The largest plant, Tunisian Medicago truncatula had the largest mRGR and the smallest plant, The Tunisian Medicago polvmorpha had the lowest mRGR. The derived parameters, leaf area ratio (LAR) and unit leaf ratio (ULR), were not consistent with the size hierarchies obtained by mRGR. The Root to shoot ratio (R/S) varied among the species. The Tunisan Medicago truncatula had high root to shoot ratios at low temperature and its R/S ratio decreased at higher temperature. Slow growing species had high R/S ratio. Medicago laciniata was an exception, it produced low R/S ratio because of its long, and thin root system. / Graduation date: 1991

Winter losses challenge the adoption of Miscanthus as a biomass crop in northern regions. This study was conducted to investigate the winter survival and yield potential of Miscanthus in Ontario. Twenty Miscanthus entries representing diploid M. sacchariflorus x M. sinensis hybrids, triploid M. x giganteus hybrids and diploid M. sinensis were established in 2008 at three locations in Ontario. First year winter survival ranged from 8-100% in Leamington, 0-100% in Elora and 0-89% in Kemptville. No difference in winter survival potential of the three species groups was observed in Leamington or Elora, but a diploid hybrid was significantly greater than all others in Kemptville. Establishment year culm height and basal circumference, and second year spring regrowth timing and biomass yield were associated with winter survival. Overall, winter severity increased from Leamington to Kemptville. The diploid hybrids were most winter hardy, followed by M. x giganteus types, followed by M. sinensis, though all had equivalent survival potential in Leamington and Elora. / Mendel Biotechnology

A system incorporating a prototype automatic thermoelectric cooling method with computer-based recording of aphid supercooling points was developed and formed the basis of cold hardiness assessment. Under laboratory culture all developmental stages of Myzus persicae had a mean inherent supercooling potential below -20°C, with first instar nymphs the most cold hardy. When maintained at 5°C, younger instars demonstrated acclimation ability unlike adult aphids, and in an insecticide resistant strain, adults lost cold hardiness. When in contact with surface moisture, the majority of aphids did not experience inoculative nucleation. Sexual morphs of M. persicae possessed supercooling ability comparable with laboratory maintained parthenogenetic morphs; eggs supercooled to below -30°C. Seasonal studies of supercooling ability demonstrated that all aphid stages were most cold hardy in summer. Younger instars showed natural acclimatisation and were cold hardy throughout the year. Overall adults lost cold hardiness as winter progressed, exhibiting bimodal supercooling point distributions in two winters, with distinct high (HG) and low (LG) groups and mean supercooling points of approximately -20°C and -10 oe respectively. Clonal differences and adult age did not account for this pattern. Following experimental starvation at 5°C, first instars of M. persicae maintained extensive supercooling potential but adults exhibited losses of cold hardiness comparable with those in natural overwintering populations, suggesting that feeding may be necessary to maintain adult cold hardiness levels during winter. Subsequent starvation experiments did not reproduce the dramatic losses of cold hardiness implying that the feeding influence was more complex than the availability of food per se. In a series of host transfer experiments the mean supercooling point of Aphis fabae adults could be shifted by over 10°C, increasing when they fed on beans and reduced when transferred back to spindle; the LG (spindle/poor supercoolers) to HG (bean/good supercoolers) shift was more difficult to achieve and suggested a nucleating agent in spindle sap. Trimethylsilyl derivatised carbohydrate extracts of M. persicae and A. fabae were analysed by capillary gas-liquid chromatography. Glucose, glycerol, fructose, mannitol, sucrose, and trehalose were detected in samples of both species, together with trace amounts of unidentified carbohydrates in M. persicae samples. Dulcitol was present in spindle-fed A. fabae only. There was no obvious correlation between carbohydrate content and supercooling ability but high total percentage body carbohydrate levels were revealed and may have a solute effect, enhancing inherent supercooling potential and dependent on carbohydrate-rich sap intake. Laboratory cultured A. fabae were capable of extensive supercooling, as were individuals collected from summer herbaceous hosts; first instars were the most cold hardy. When associated with the primary host, spindle, all aphids showed poor supercooling potential, less than -15°C; overwintering eggs were capable of supercooling to below -30°C and acclimatised in winter. Eggs and oviparae were not subject to inoculative nucleation. Preliminary experiments to relate supercooling ability to mortality at sub-zero temperatures proved inconclusive and were terminated when temperature shock and/or desiccation were thought to have induced premature mortality. The results demonstrate that the cold hardiness characteristics of M. persicae are atypical of those observed in other freezing-susceptible arthropods. It is proposed that continued feeding during mild winters maintains cold hardiness levels in adult M. persicae and this influence may provide a possible explanation for the successful anholocyclic overwintering of this aphid during such winters. Avenues of research to further investigate this proposal are suggested.

Hardiness é um conceito que está cada vez mais sendo usado com a finalidade de explicar as possíveis diferenças individuais no enfrentamento do stress. Os instrumentos de medida de hardiness são em língua inglesa, dos quais optou-se pela adaptação cultural da Hardiness Scale, original dos Estados Unidos da América, de autoria de Bartone, Ursano, Wright e Ingraham (1989). Sua finalidade é avaliar o quanto de atitudes Hardy as pessoas têm no enfrentamento de situações estressantes. É uma escala do tipo Likert, com escores que variam de 0 (nada verdadeiro) a 3 (totalmente verdadeiro), possui 30 itens que são distribuídos em três domínios (Compromisso, Controle e Desafio). Os objetivos deste estudo foram realizar a adaptação cultural da Hardiness Scale para a língua portuguesa do Brasil; avaliar a validade de construto e a confiabilidade da versão adaptada. As etapas propostas por Ferrer et al (1996) foram obedecidas, a saber: 1. Tradução para língua portuguesa (Consenso: Versão I em Português); 2. Avaliação pelo Comitê de Revisão (Versão II em Português); 3. Retradução (Back translation); 4. Avaliação semântica dos itens (Versão III em Português); 5. Pré-teste (Versão Final Português) e 6. Análise das propriedades da medida adaptada. A coleta de dados da aplicação do instrumento foi realizada junto aos enfermeiros do serviço público de saúde de dois municípios do interior do estado de São Paulo, totalizando 71 participantes. A confiabilidade interna medida com o uso de Alpha de Cronbach obteve os seguintes valores: para a composição da escala foi de 0,732, enquanto que os domínios apresentaram alfa de 0,683 para Compromisso, 0,632 para Controle e 0,441 para Desafio. Na análise da Escala de Hardiness adaptada quanto à validade de construto obteve-se relação positiva e significante com o Inventário de Estratégias de Coping de Folkman e Lazarus, e negativa e significante com o Inventário de Depressão de Beck. Pode-se concluir que a Escala de Hardiness apresenta-se adaptada para a língua portuguesa do Brasil, com consistência interna satisfatória e validade de construto na população estudada e seus achados corroboram com a literatura corrente / Hardiness is a concept that is increasingly being used in order to explain possible individual differences in the coping of stress. The instruments for measuring hardiness are in English language and of witch opted for the cultural adaptation of the Hardiness Scale, original United States of America, by Bartone, Ursano, Wright and Ingraham (1989). Its purpose is to assess how much Hardy attitudes the people have in coping with stressful situations. It is a Likert-type scale, with scores ranging from 0 (not true) to 3 (completely true), it contains 30 items that are distributed in three domains (Commitment, Control and Challenge). The objectives of this study were to perform cultural adaptation of the Hardiness Scale into Portuguese of Brazil, to evaluate the reliability and construct validity of the adapted version. The steps proposed by Ferrer et al. (1996) were followed, namely: 1. Translation to Portuguese language (Consensus: Version I in Portuguese), 2. Evaluation by the Committee of Review (Version II in Portuguese), 3. Back translation, 4. Semantics evaluation of the items (Version III in Portuguese), 5. Pre-test (Final Version Portuguese) and 6. Analysis of the properties of the adapted measure. The data from the application of the instrument was conducted with nurses from the public health service in two cities country side of São Paulo State, totalizing 71 participants. The internal reliability measured by use of Cronbach\'s Alpha achieved the following values: for the composition of the scale was .732, while the areas showed Commitment to .683, .632 for Control and 0.441 to Challenge. In the analysis of the adapted Hardiness Scale concerning the validity of construct obtained significant and positive relation with the Inventory of Coping Strategies of Folkman and Lazarus, and negative and significant with the Beck Depression Inventory. It can be concluded that the Hardiness Scale, it is adapted to the Portuguese language of Brazil, with satisfactory internal consistency and construct validity in the studied population and its findings corroborate with current literature

Hardiness é um conceito que está cada vez mais sendo usado com a finalidade de explicar as possíveis diferenças individuais no enfrentamento do stress. Os instrumentos de medida de hardiness são em língua inglesa, dos quais optou-se pela adaptação cultural da Hardiness Scale, original dos Estados Unidos da América, de autoria de Bartone, Ursano, Wright e Ingraham (1989). Sua finalidade é avaliar o quanto de atitudes Hardy as pessoas têm no enfrentamento de situações estressantes. É uma escala do tipo Likert, com escores que variam de 0 (nada verdadeiro) a 3 (totalmente verdadeiro), possui 30 itens que são distribuídos em três domínios (Compromisso, Controle e Desafio). Os objetivos deste estudo foram realizar a adaptação cultural da Hardiness Scale para a língua portuguesa do Brasil; avaliar a validade de construto e a confiabilidade da versão adaptada. As etapas propostas por Ferrer et al (1996) foram obedecidas, a saber: 1. Tradução para língua portuguesa (Consenso: Versão I em Português); 2. Avaliação pelo Comitê de Revisão (Versão II em Português); 3. Retradução (Back translation); 4. Avaliação semântica dos itens (Versão III em Português); 5. Pré-teste (Versão Final Português) e 6. Análise das propriedades da medida adaptada. A coleta de dados da aplicação do instrumento foi realizada junto aos enfermeiros do serviço público de saúde de dois municípios do interior do estado de São Paulo, totalizando 71 participantes. A confiabilidade interna medida com o uso de Alpha de Cronbach obteve os seguintes valores: para a composição da escala foi de 0,732, enquanto que os domínios apresentaram alfa de 0,683 para Compromisso, 0,632 para Controle e 0,441 para Desafio. Na análise da Escala de Hardiness adaptada quanto à validade de construto obteve-se relação positiva e significante com o Inventário de Estratégias de Coping de Folkman e Lazarus, e negativa e significante com o Inventário de Depressão de Beck. Pode-se concluir que a Escala de Hardiness apresenta-se adaptada para a língua portuguesa do Brasil, com consistência interna satisfatória e validade de construto na população estudada e seus achados corroboram com a literatura corrente / Hardiness is a concept that is increasingly being used in order to explain possible individual differences in the coping of stress. The instruments for measuring hardiness are in English language and of witch opted for the cultural adaptation of the Hardiness Scale, original United States of America, by Bartone, Ursano, Wright and Ingraham (1989). Its purpose is to assess how much Hardy attitudes the people have in coping with stressful situations. It is a Likert-type scale, with scores ranging from 0 (not true) to 3 (completely true), it contains 30 items that are distributed in three domains (Commitment, Control and Challenge). The objectives of this study were to perform cultural adaptation of the Hardiness Scale into Portuguese of Brazil, to evaluate the reliability and construct validity of the adapted version. The steps proposed by Ferrer et al. (1996) were followed, namely: 1. Translation to Portuguese language (Consensus: Version I in Portuguese), 2. Evaluation by the Committee of Review (Version II in Portuguese), 3. Back translation, 4. Semantics evaluation of the items (Version III in Portuguese), 5. Pre-test (Final Version Portuguese) and 6. Analysis of the properties of the adapted measure. The data from the application of the instrument was conducted with nurses from the public health service in two cities country side of São Paulo State, totalizing 71 participants. The internal reliability measured by use of Cronbach\'s Alpha achieved the following values: for the composition of the scale was .732, while the areas showed Commitment to .683, .632 for Control and 0.441 to Challenge. In the analysis of the adapted Hardiness Scale concerning the validity of construct obtained significant and positive relation with the Inventory of Coping Strategies of Folkman and Lazarus, and negative and significant with the Beck Depression Inventory. It can be concluded that the Hardiness Scale, it is adapted to the Portuguese language of Brazil, with satisfactory internal consistency and construct validity in the studied population and its findings corroborate with current literature

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by Li Wing Yen Francisca. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 94-105). / Abstracts in English and Chinese. / Thesis committee --- p.i / Statement --- p.ii / Abstract --- p.iii / Chinese Abstract --- p.v / Acknowledgemnets --- p.vii / Abbreviations --- p.ix / Table of contents --- p.xii / List of figures --- p.xvi / List of tables --- p.xvii / Chapter 1. --- General Introduction / Chapter 1.1 --- Introduction to oxidative stress / Chapter 1.1.1 --- Introduction to Reactive Oxygen Species --- p.1 / Chapter 1.1.2 --- Major sites of ROS production / Chapter 1.1.2.1 --- Chloroplast --- p.4 / Chapter 1.1.2.2 --- Mitochondria --- p.4 / Chapter 1.2 --- Regulation of intercellular ROS content in plant cells / Chapter 1.2.1 --- Enzymatic defense ofROS --- p.6 / Chapter 1.2.1.1 --- Superoxide dismutases --- p.6 / Chapter 1.2.1.2 --- "Ascorbate peroxidase, Glutathione reductase and the Ascorbate-Glutathione cycle" --- p.7 / Chapter 1.2.1.3 --- Catalase --- p.11 / Chapter 1.2.1.4 --- Alternative oxidase --- p.11 / Chapter 1.2.2 --- Non-enzymatic / Chapter 1.2.2.1 --- Ascorbate and Glutathione --- p.12 / Chapter 1.2.2.2 --- α-tocopherol --- p.12 / Chapter 1.3 --- "Salt, dehydration and oxidative stress" / Chapter 1.3.1 --- Oxidative stress is induced when plants were under salt stress --- p.13 / Chapter 1.3.2 --- Oxidative stress is induced when plants were under dehydration stress --- p.14 / Chapter 1.4 --- ROS scavenging: the road to achieve multiple-stress tolerance? --- p.16 / Chapter 1.5 --- Purple acid phosphatase and its relationship with oxidative stress in plants / Chapter 1.5.1 --- General introduction to plants purple acid phosphatase (PAP) --- p.20 / Chapter 1.5.2 --- Purple acid phosphatases that found to be involved in ROS scavenging in plants --- p.21 / Chapter 1.6 --- Previous studies in GmPAP3 --- p.23 / Chapter 1.7 --- Hypothesis and significance of this project --- p.25 / Chapter 2. --- Materials and methods / Chapter 2.1 --- Materials / Chapter 2.1.1 --- "Plants, bacterial strains and vectors." --- p.26 / Chapter 2.1.2 --- Chemicals and reagents --- p.27 / Chapter 2.1.3 --- Commercial kits --- p.28 / Chapter 2.1.4 --- Primers and adaptors --- p.29 / Chapter 2.1.5 --- Equipments and facilities used --- p.31 / Chapter 2.1.6 --- "Buffer, solution, gel and medium" --- p.31 / Chapter 2.1.7 --- Software --- p.31 / Chapter 2.2 --- Methods / Chapter 2.2.1 --- Molecular techniques / Chapter 2.2.1.1 --- Bacterial cultures for recombinant DNA and plant transformation --- p.32 / Chapter 2.2.1.2 --- Recombinant DNA techniques --- p.32 / Chapter 2.2.1.3 --- "Preparation and transformation of DH5α, DE3 and Agrobacterium competent cells" --- p.33 / Chapter 2.2.1.4 --- Gel electrophoresis --- p.36 / Chapter 2.2.1.5 --- DNA and RNA extraction --- p.37 / Chapter 2.2.1.6 --- Generation of single-stranded DIG-labeled PCR probes --- p.38 / Chapter 2.2.1.7 --- Testing the concentration of DIG-labeled probes --- p.40 / Chapter 2.2.1.8 --- Northern blot analysis --- p.40 / Chapter 2.2.1.9 --- PCR techniques --- p.41 / Chapter 2.2.1.10 --- Sequencing --- p.42 / Chapter 2.2.2 --- Plant cell culture and transformation / Chapter 2.2.2.1 --- Arabidopsis thaliana --- p.43 / Chapter 2.2.2.2 --- Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) cells --- p.44 / Chapter 2.2.3 --- Growth and treatment conditions for plants / Chapter 2.2.3.1 --- Growth and salt treatment condition of soybean samples for gene expression studies of GmPAPS --- p.45 / Chapter 2.2.3.2 --- Root assay of GmPAP3 transgenic Arabidopsis thaliana --- p.46 / Chapter 2.2.4 --- "Immunolabeling, mitochondria integrity, ROS detection and confocal microscopy" / Chapter 2.2.4.1 --- Immunolabeling of GmPAP3-T7 transgenic cell lines --- p.47 / Chapter 2.2.4.2 --- Mitochondria integrity --- p.48 / Chapter 2.2.4.3 --- Detection of Reactive oxygen species (ROS) --- p.48 / Chapter 2.2.4.4 --- Confocal microscopy --- p.49 / Chapter 2.2.4.5 --- Images processing and analysis --- p.49 / Chapter 2.2.5 --- Statistical analysis --- p.50 / Chapter 3. --- Results / Chapter 3.1 --- "Expression of GmPAP3 was induced by NaCl stress, oxidative stress, and dehydration stress" --- p.51 / Chapter 3.2 --- Establishment of GmPAP3-T7 fusion transgenic cell lines / Chapter 3.2.1 --- Subcloning of GmPAP3-T7 into the binary vector system W104 --- p.53 / Chapter 3.2.2 --- Transformation of W104-GmPAP3-T7 into tobacco BY-2 cells --- p.56 / Chapter 3.3 --- Establishment of GmPAP3 trangenic cell lines / Chapter 3.3.1 --- Subcloning of GmPAP3 into the binary vector system W104 --- p.58 / Chapter 3.3.2 --- Transformation of W104-GmPAP3 into tobacco BY-2 cells --- p.58 / Chapter 3.4 --- Establishment of GmPAP3 transgenic Arabidopsis thaliana / Chapter 3.4.1 --- Transformation of W104-GmPAP3 into Arabidopsis thaliana --- p.61 / Chapter 3.5 --- Colocalization of GmPAP3 with MitoTracker-orange --- p.66 / Chapter 3.6 --- Effect of expressing GmPAP 3 on mitochondria integrity of BY-2 cells under NaCl and dehydration stress. --- p.71 / Chapter 3.7 --- Effect of expressing GmPAP3 on ROS production in BY-2 cells under salt and PEG treatment --- p.75 / Chapter 3.8 --- Effect of expressing GmPAP3 in Arabidopsis thaliana under salt stress --- p.81 / Chapter 4. --- Discussion / Chapter 4.1 --- Gene expression profile of GmPAP3 --- p.83 / Chapter 4.2 --- Subcellular localization of GmPAP3 --- p.84 / Chapter 4.3 --- Functional tests of GmPAP 3 transgenic BY-2 cells / Chapter 4.3.1 --- GmPAP3 could protect the plant cells' mitochondria integrity when under salt and dehydration stress --- p.86 / Chapter 4.3.2 --- Expressing GmPAPS in tobacco BY-2 cells were able to reduce the production ofROS under salt and dehydration stresses --- p.88 / Chapter 4.4 --- Functional tests of GmPAP3 transgenic Arabidopsis --- p.91 / Chapter 5. --- Conclusion and perspectives --- p.92 / References --- p.94 / Appendix I: Restriction and modifying enzymes --- p.106 / Appendix II: Chemicals --- p.107 / Appendix III: Commercial kits --- p.111 / Appendix IV: Equipments and facilities used --- p.112 / "Appendix V: Buffer, solution, gel and medium formulation" --- p.113

Thesis (M.S.)--Miami University, Dept. of Zoology, 2007. / Title from first page of PDF document. Includes bibliographical references (p. 17-20).