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Sabe-se que o flúor causa alterações químicas e celulares no osso, dependendo da dosagem e do tempo de exposição a esse elemento. Já foi demonstrado que alterações clínicas importantes, como a osteoporose, osteopetrose e esclerose óssea, podem ser geradas pela ingestão de flúor. Sabe-se que o flúor pode guiar os osteoblastos ao crescimento, sob doses e tempos de exposição específicos. A sensibilidade dos tecidos mineralizados ao fluoreto responde a mecanismos genéticos inerentes a cada população. Dentro de uma mesma espécie, diferentes linhagens apresentam um comportamento distinto frente a esse fármaco. Durante várias décadas, os estudos com fluoretos resumiram-se ao fluoreto de sódio, amplamente utilizado na prevenção das cáries dentárias. Recentemente, outras fontes de fluoretos como TiF4 e SnF2 têm sido buscadas, com intuito de potencializar as vantagens desse elemento. Porém não existem estudos ainda sobre o efeito do AlF3 sobre o tecido ósseo até a presente data. Desse modo, achamos oportuno investigar seu papel, quando administrado a células ósseas e para tal, investigamos a viabilidade e padrão de atividade das fosfatases desse sal (AlF3) quando administrado às células da linhagem pré-osteblástica MC3T3, em comparação ao NaF. Além disso, sabendo que diferentes células apresentam comportamento distinto ao tratamento com fluoretos, estudamos o papel do NaF quando administrado a duas linhagens celulares de camundongos com densidades ósseas distintas e com diferentes susceptibilidades ao fluoreto: C3H/HeJ e C57BL/6J (C3H e C57, alta e baixa densidades ósseas, respectivamente). As células também foram avaliadas quanto à viabilidade celular e ao perfil das fosfatases. Todos os resultados foram submetidos à Análise de Variância a três critérios e nos permitiu identificar que entre os sais de fluoreto, apenas o NaF promoveu alterações na viabilidade celular das células MC3T3. O AlF3 não exerceu influência na atividade da fosfatase alcalina quando consideramos as células pré-osteoblásticas, no entanto, o efeito desse fluoreto sobre a atividade das fosfatases ácidas totais e ácida resistente ao tartarato ocorre de modo dicotômico. Quando consideramos uma população celular heterogênea, coletada de calvária, como foi o caso das linhagens C3H e C57, verificamos que o NaF interferiu tanto sobre a proliferação, quanto a atividade das fosfatases de modo bastante distinto para ambas linhagens celulares. Assim concluimos que o NaF é capaz de exercer efeitos mais diversificados em diferentes tipos celulares, alterando viabilidade celular e atividade de enzimas; em contrapartida, o AlF3 produz efeitos mais especifícos e delimitados em pré-osteoblastos. / It is well known that fluoride causes chemical and cellular alteration on bone tissue, depending on the dosage and time of exposure to this element. Several studies have been conducted to show that some bone diseases, such as osteoporosis, osteopetrosis and esclerosis, can occur as a consequence of the excessive fluoride uptake. Fluoride can lead osteoblast to proliferate, under certain conditions as time and dosage. The same way, excessive dosages of fluoride can alter protein activity in osteoblasts, by changing the expression of retated genes. Sensitivity of mineralized tissues to fluoride depends on a genetic background inherent to each population. Within the same specie, different lineages present distint behavior to the ingestion of this íon. Over several decades, many researches focused on the study of NaF as the only source of fluoride, once it is wide spread used to the prevention of dental caries. More recently, different sources of fluoride have been assessed, like TiF4 e SnF2</sub, with the main aim to enhance biochemical and physical benefits of this element. Despite of this, none of these formulations have been tested to evaluate their effect on the bone tissue. Thus, we believe it is advisable to investigate viability and phosphatases activities of pre-osteoblast MC3T3 cells treated with Aluminium fluoride, compared to NaF treatments. Besides this, facing the knowledge that different cell lines have distinct behavior when treated with fluoride, we used two inbred strains of mice with distint mineral bone densities, C3H/HeJ (C3H, high bone density) and C57BL/6J (C57, low bone density), to assess the viability and phosphatases activities after the treatment with NaF. Overall results were tested by 3 way- Anova. AlF3 did not exibit influence over on alkaline phosphatase when pre-osteoblast cells are considered, but the effect of this fluoride salt on the activity of total and tartarate resistant acid phosphatases ocurrs on a dicotomic way. When we consider a mixed cell population, collected from calvária as for the C3H and C57 assays, NaF did interfered as with the proliferation rate as phosphatase activities on a very distinct fashion between the two lineages. Thus, it is possible to conclude that NaF is able to exert variable effects in different cell types and enzyme activities; on the other hand, AlF3 effects are more specific and surrounded in pre-osteblastic cells.

Sabe-se que o flúor causa alterações químicas e celulares no osso, dependendo da dosagem e do tempo de exposição a esse elemento. Já foi demonstrado que alterações clínicas importantes, como a osteoporose, osteopetrose e esclerose óssea, podem ser geradas pela ingestão de flúor. Sabe-se que o flúor pode guiar os osteoblastos ao crescimento, sob doses e tempos de exposição específicos. A sensibilidade dos tecidos mineralizados ao fluoreto responde a mecanismos genéticos inerentes a cada população. Dentro de uma mesma espécie, diferentes linhagens apresentam um comportamento distinto frente a esse fármaco. Durante várias décadas, os estudos com fluoretos resumiram-se ao fluoreto de sódio, amplamente utilizado na prevenção das cáries dentárias. Recentemente, outras fontes de fluoretos como TiF4 e SnF2 têm sido buscadas, com intuito de potencializar as vantagens desse elemento. Porém não existem estudos ainda sobre o efeito do AlF3 sobre o tecido ósseo até a presente data. Desse modo, achamos oportuno investigar seu papel, quando administrado a células ósseas e para tal, investigamos a viabilidade e padrão de atividade das fosfatases desse sal (AlF3) quando administrado às células da linhagem pré-osteblástica MC3T3, em comparação ao NaF. Além disso, sabendo que diferentes células apresentam comportamento distinto ao tratamento com fluoretos, estudamos o papel do NaF quando administrado a duas linhagens celulares de camundongos com densidades ósseas distintas e com diferentes susceptibilidades ao fluoreto: C3H/HeJ e C57BL/6J (C3H e C57, alta e baixa densidades ósseas, respectivamente). As células também foram avaliadas quanto à viabilidade celular e ao perfil das fosfatases. Todos os resultados foram submetidos à Análise de Variância a três critérios e nos permitiu identificar que entre os sais de fluoreto, apenas o NaF promoveu alterações na viabilidade celular das células MC3T3. O AlF3 não exerceu influência na atividade da fosfatase alcalina quando consideramos as células pré-osteoblásticas, no entanto, o efeito desse fluoreto sobre a atividade das fosfatases ácidas totais e ácida resistente ao tartarato ocorre de modo dicotômico. Quando consideramos uma população celular heterogênea, coletada de calvária, como foi o caso das linhagens C3H e C57, verificamos que o NaF interferiu tanto sobre a proliferação, quanto a atividade das fosfatases de modo bastante distinto para ambas linhagens celulares. Assim concluimos que o NaF é capaz de exercer efeitos mais diversificados em diferentes tipos celulares, alterando viabilidade celular e atividade de enzimas; em contrapartida, o AlF3 produz efeitos mais especifícos e delimitados em pré-osteoblastos. / It is well known that fluoride causes chemical and cellular alteration on bone tissue, depending on the dosage and time of exposure to this element. Several studies have been conducted to show that some bone diseases, such as osteoporosis, osteopetrosis and esclerosis, can occur as a consequence of the excessive fluoride uptake. Fluoride can lead osteoblast to proliferate, under certain conditions as time and dosage. The same way, excessive dosages of fluoride can alter protein activity in osteoblasts, by changing the expression of retated genes. Sensitivity of mineralized tissues to fluoride depends on a genetic background inherent to each population. Within the same specie, different lineages present distint behavior to the ingestion of this íon. Over several decades, many researches focused on the study of NaF as the only source of fluoride, once it is wide spread used to the prevention of dental caries. More recently, different sources of fluoride have been assessed, like TiF4 e SnF2</sub, with the main aim to enhance biochemical and physical benefits of this element. Despite of this, none of these formulations have been tested to evaluate their effect on the bone tissue. Thus, we believe it is advisable to investigate viability and phosphatases activities of pre-osteoblast MC3T3 cells treated with Aluminium fluoride, compared to NaF treatments. Besides this, facing the knowledge that different cell lines have distinct behavior when treated with fluoride, we used two inbred strains of mice with distint mineral bone densities, C3H/HeJ (C3H, high bone density) and C57BL/6J (C57, low bone density), to assess the viability and phosphatases activities after the treatment with NaF. Overall results were tested by 3 way- Anova. AlF3 did not exibit influence over on alkaline phosphatase when pre-osteoblast cells are considered, but the effect of this fluoride salt on the activity of total and tartarate resistant acid phosphatases ocurrs on a dicotomic way. When we consider a mixed cell population, collected from calvária as for the C3H and C57 assays, NaF did interfered as with the proliferation rate as phosphatase activities on a very distinct fashion between the two lineages. Thus, it is possible to conclude that NaF is able to exert variable effects in different cell types and enzyme activities; on the other hand, AlF3 effects are more specific and surrounded in pre-osteblastic cells.

The Large Hadron Collider (LHC) has provided, and will continue to provide, data for collisions at the highest energies ever seen in a particle accelerator. A strong knowledge of the properties of amplitudes for Quantum Chromodynamics in the High Energy Limit is therefore important to interpret this data. We study this limit in the context of the High Energy Jets (HEJ) formalism. This formalism resums terms in the perturbative expansion of the cross-section that behave like αn/s log (s/-t)ⁿ¯¹, which are enhanced in this limit. Understanding this region is particularly important in certain key analyses at the LHC: for example, Higgs-boson- plus-dijet analyses where cuts are applied to pick out events with a large mjj and in many searches for new physics. In this thesis, we discuss two directions in which HEJ's accuracy has been improved. Firstly, we look at adding descriptions of partonic subprocesses which are formally sub-leading in the jet cross-section but Leading Logarithmic (LL) in the particular subprocess itself. This required the derivation of new effective vertices that describe the emission of a quark/anti-quark pair in a way that is consistent with the resummation procedure. The inclusion of such processes reduces HEJ's dependence on fixed-order calculations and marks an important step towards full Next-to-Leading Logarithmic (NLL) accuracy in the inclusive dijet cross-section. The second extension was to improve our description of events involving the emission of a Higgs boson along with jets. Specifically, we derive new effective vertices which keep the full dependence on the quark mass that appears in the loops that naturally arise in such amplitudes. The formalism is also simple enough to allow for any number of extra nal state jets in the process. Therefore, HEJ is unique in its ability to provide predictions for high-multiplicity Higgs-plus-jets processes with full nite quark mass e ects. Such a calculation is far beyond the reach of any xed order approach.

The purpose of this study is to see how death and the grief process is explained in three children's books with death as a motif. I will also describe how to look at children's literature as a teaching material when dealing with existential questions with the youngest children in school. The information I gathered from these books shows that death is often mentioned in a connection to an older person who dies from ageing or a parent who dies in the event of a fatal disease. In some of these books is life after death described with a religious belief, most from Christianity. They all mediates hope and a view of death as a continuation, meaning that something else is happening after your life as a human. I also come to the fact that the pictures mediate important messages and play a decisive part in the perception of the story. I concluded that all three books are useful when you are teaching children about death and grief. But they should be used for different purposes and in different contexts in the classroom. / Syftet med denna studie är att se hur döden och sorgearbetet framställs i tre skönlitterära barnböcker med döden som tema. Jag kommer även att ta upp hur man kan se på barnlitteratur som ett undervisningsmaterial när man arbetar med livsfrågor med de yngsta barnen i skolan. Resultatet visar att döden beskrivs i form av att det är en äldre person som dör av ålder eller i samband med att en förälder dör av en sjukdom. I böckerna kan man även se kopplingar till religion och främst till kristendomen. Böckerna kan tänkas förmedla hopp om att döden är en fortsättning och att det sker något mer efter ditt liv som människa. Jag kommer även fram till att bilderna förmedlar viktiga budskap och har avgörande roll för hur man uppfattar berättelsen. Jag kommer fram till att samtliga böcker är användbara när man arbetar med döden och sorg med små barn, men att man bör använda böcker på olika sätt och i olika syften i undervisningen.

This diploma thesis called "The analysis of selected choral compositions by Jakub Jan Ryba" deals primarily with his religious works. The introductory chapter briefly describes Ryba's journey to music and the activity of a composer and the text is also based on Ryba's own biography published in 2005 titled My Life and Music. The thesis concerns an extant autographic list of his works dated to 1801 and an autographic fragment of an incipit catalogue of compositions dated from 1774 to 1796 including an incipit of Czech Christmas Mass "Hey, Master!" dated back to 1795. Both lists were compared with two electronic music databases: the catalogue of the department of music history, National Museum - the Czech Museum of Music and the musical incipit catalogue of the National Library of the Czech Republic. The electronic databases were further compared with the thematic catalogue which is a part of the monography titled Jakub Jan Ryba published in 1963. The object of interest was also the quantity of autographs and transcripts and the prints of the works by Jakub Jan Ryba which have been issued from the beginning of the 19th century till present. Regarding the results of the research, it was proved that only a part of Ryba's work has survived up till now since Ryba himself presents 1083 compositions, both...