This thesis presents an adaptive tuning system that can be described as a dynamic Just Intonation tuning system, being compatible with equally tempered instruments. The tuning system is called Hermode Tuning (HMT) and the tuning used as comparison for evaluation is the standardized western tuning, the equal tempered tuning. This study investigates preferences for these two musical tuning systems, depending on whether the tunings are presented on a piano or with woodwind instruments. A listening test was done with students at the Falun Conservatory of Music, including both a vertical listening (intervalls) and a horizontal listening (cadenses and musical compositions) of Hermode tuned musical material. Overall the results showed no significant preferences for either tuning system irrespectively of what instrument it was presented with. The clearest results was that of a misjudged just intonated perfect third on the piano and a preference for an adaptively tuned piano presented in a simple harmonic structure, with a parameter setting of HMT 70%. Materials for comparison was partly taken from Hermode´s own website, but overall the attitude towards these sequenses (using a likert scale of one to five) showed a low expected value. This shows the complexity of the topic and no general conclusions regarding the choice of intonation or tuning system could be done for the presented material.