Cette thèse a pour but d’étudier le transport de porteur de charge au sein de polymères conjugués, avec comme finalité d’identifier les propriétés des appareils d’électronique organique appropriées pour des applications dans la spintronique organique. Nous avons analysé des échantillons planaires, de géométries latérales, qui offrent la possibilité d’étudier les propriétés de transport sous l’application de différents stimulus et la détection le transport de longue distance du moment angulaire (spin), au sein de semi-conducteurs organiques (OSC). Dans cette configuration, des critères bien établis doivent être satisfait pour réaliser le transport diffusif d’un courant de spin polarisé au travers d’un matériel organique. Nous avons analysé ces diffèrent critères et trouvé des matériaux dont les propriétés physiques fournissent une solution satisfaisante. Le résultat de ce travail fut la création de transistors à effet de champ organiques dont les propriétés répondent au besoin des applications de spintronique. / In this thesis, charge carrier transport in conjugated polymers is studied with the aim to identify organic electronics devices properties suitable for applications in organic spintronics. We investigate planar samples, in a lateral geometry, which offer the possibility to study transport properties under the application of different stimuli and to detect long-range spin transport in OSCs. In this configuration, well-established criteria must be satisfied to realize diffusive-like transport of a spin-polarized current through an organic material. We analyse these criteria and find possible materials properties solutions. The outcome is the realization of organic field-effect transistors with properties ad hoc for spintronics applications.

The work presented in this thesis focuses on the investigation and improvement of the window stack of layers for thin film CdTe solar cells fabricated in the Center for Renewable Energy Systems Technology (CREST) laboratories. In particular the aim was to change the standard structure including TCO, high resistive transparent (HRT)layer and CdS which is limited by the low transparency of the CdS layer, to a better performing one. The first result chapter of the thesis describes the study of ZnO HRT layers. ZnO thin films were deposited by radio frequency (RF) magnetron sputtering with different structural, optical and electrical properties which were characterized by X-ray diffraction, electron microscopy, spectrophotometry, Hall Effect method and 4-point probe. ZnO films were then incorporated in CdTe solar cells with the structure: FTO/ZnO/CdS/CdTe/Au back contact and the performance of these devices were compared with the film properties to single out trends and identify optimal film characteristics. By varying the deposition pressure of ZnO films, it was possible to increase their transparency and significantly increase their resistivity. While better transparency positively affected the solar cell current density output and efficiency, the resistivity of ZnO films did not show any clear impact on device efficiency. By increasing the deposition temperature the ZnO film grain size was increased. Increased FF was observed in devices incorporating ZnO layers with bigger grains, although this gain was partially counterbalanced by the Voc degradation, leading to a limited efficiency improvement. Finally the addition of oxygen had the main effect of increasing the resistivity of ZnO films, similarly to what happened with the increase of the sputtering pressure. In this case however, an improvement of FF, Jsc and efficiency was observed, especially at an O2/Ar ratio of 1%. By simulating the solar cells behavior with SCAPS-1D, it was found that these performance change can be explained by the variation of interface properties, precisely the amount of interface defects, rather than by bulk properties. The study presented in the second result chapter focuses on magnesium-doped zinc oxide (MZO) and the variation of its energy band structure. MZO was initially used as the HRT layer within a solar cell structure: FTO/MZO/CdS/CdTe/Au back contact. Sputtering MZO films with a target containing MgO 11 weight% and ZnO 89 weight% allowed for and increased band gap from 3.3 eV of intrinsic ZnO to 3.65 eV for MZO deposited at room temperature. Increasing the superstrate deposition temperature allowed for a further band gap increase up to 3.95 eV at 400 °C due mainly to an conduction band minimum upward shift. It was highlighted the importance to create a positive conduction band offset with the MZO layer conduction band slightly above the CdS conduction band, with an optimum found in this case to be 0.3 eV (efficiency 10.6 %). By creating a positive conduction band offset all the performance parameters (Voc, FF, Jsc, efficiency) significantly increased. One of the reasons for this improvement was found to be a diminished interface recombination due to a more ideal MZO/CdS band alignment. In the second part of this investigation the MZO was used as a replacement for the CdS in a simplified structure: FTO/MZO/CdTe/Au back contact. The concepts used to optimise the performance of these devices also involved tuning the conduction band alignment between MZO/CdTe and efficiencies of 12.5 % were achieved with a at conduction band offset. The efficiency increase was achieved mainly thanks to a better transparency of the MZO layer and a higher Jsc output, compared to devices using a CdS buffer layer. The MZO buffers have been tested in combination with different TCOs. Results are presented in the third result chapter and showed that AZO is a good alternative to FTO working effectively in combination with MZO. AZO/MZO efficiency thin film CdTe solar cells (12.6%, compared to 12.5% with FTO). It was found that increasing the IR transparency of the TCOs leads to a potentially higher Jsc. Achieving a better transparency was obtained by using TCOs with high mobility and lower carrier concentration (AZO and ITiO) and also by using a boro-aluminosilicate glass with low iron content. ITiO yielded the best opto-electrical properties among all the TCO materials. Devices incorporating ITiO however, showed lower performance then those using FTO and AZO. ITO/MZO windows also yielded poor performance. In addition, the ITO films deposited had a high carrier concentration leading to a high NIR absorption by plasma resonance and resulted not ideal for application in thin film CdTe PV.

Orientador: Celso Eduardo Benedetti / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-16T01:56:52Z (GMT). No. of bitstreams: 1 Souza_TiagoAntoniode_M.pdf: 8040684 bytes, checksum: 0b9836df8d96343f09503df5056436cd (MD5) Previous issue date: 2010 / Resumo: O cancro cítrico, causado pela bactéria Xanthomonas axonopodis pv. citri (Xac), é uma doença que afeta a maioria das espécies do gênero Citrus, ocorrendo praticamente em todos os continentes, e se destaca como uma das ameaças à citricultura brasileira. O mecanismo molecular pelo qual Xac causa o cancro não é inteiramente conhecido, entretanto, sabe-se que a bactéria ao infectar a planta, utiliza o sistema secretório tipo ??? (TTSS) para injetar proteínas de patogenicidade, entre elas PthAs da família AvrBs3/PthA. Quando expresso na célula hospedeira, PthA induz lesões características do cancro como hipertrofia e hiperplasia. Estudos recentes demonstram que membros dessa família atuam como fatores de transcrição. Portanto, a elucidação de como PthA ativa a transcrição é de grande importância para o entendimento do seu mecanismo de ação e desenvolvimento das lesões do cancro. Neste contexto, o presente projeto teve como objetivo caracterizar interações entre a proteína PthA de Xac e as proteínas CsARF (Auxin Response Factor) e CsHMG (High-mobility group) de laranja doce (Citrus sinensis), previamente identificadas em ensaios de duplo híbrido de leveduras. CsARF tem elevada similaridade com AtARF2, um repressor transcricional envolvido na via de sinalização por auxinas. Hormônios vegetais desempenham um importante papel na interação planta-patógeno e em nosso laboratório verificamos que auxinas são importantes para o desenvolvimento dos sintomas do cancro. CsARF foi capaz de interagir com a maioria das variantes de PthA tanto in vitro quanto em ensaios de duplo-híbrido de leveduras. A interação de CsARF com PthA se dá através dos domínios C-terminal Aux/IAA e B3 de ligação ao DNA. Verificamos que o promotor do gene de uma expansina de citros, induzido por Xac e auxina, apresenta possíveis sítios de ligação das proteínas CsARF e PthA. Dados de EMSA indicam que PthA e CsARF ligam em sítios adjacentes no promotor da expansina de citros e que a interação de PthA com CsARF poderia deslocá-la do promotor. A proteína CsHMG é semelhante a AtHMGB1 de Arabidopsis thaliana, envolvida em crescimento celular. CsHMG interagiu com todas as variantes de PthA, sendo que essa interação envolve uma região rica em leucinas (LRR), idêntica nas quatro variantes de PthA. Verificou-se também que CsHMG é capaz de ligar DNA de forma inespecífica. Por outro lado, CsHMG ligou RNA in vitro, com especificidade para RNAs ricos em uridina (poly-U). Como PthA age como fator de transcrição eucarioto, não é surpreendente que proteínas do hospedeiro envolvidas com regulação gênica sejam capazes de interagir com esse efetor, sugerindo um novo modo de ação de proteínas efetoras bacterianas. / Abstract: Citrus canker disease, caused by Xanthomonas axonopodis pv. citri (Xac), affects almost all citrus species and represents a major threat to the Brazilian citriculture. The molecular mechanism by which Xac causes citrus canker disease is poorly understood, however the bacterium injects pathogenicity proteins through a type III secretion system (TTSS) including proteins of AvrBs3/PthA family proteins. When transiently expressed in host cells, PthAs alter transcription of the host cell to the benefit of the pathogen, leading to the development of the cancer lesions, including hypertrophy and hyperplasia. These proteins are thought to acts as eukaryotic transcriptional factors, binding and activating directly promoters of host genes. Therefore, elucidating how activates PthA transcription is very important to understanding the mechanisms governing the development of canker lesions. To elucidate how PthA activates transcription and to establish its molecular mode of action, a two-hybrid approach was used to identify host proteins that interact with PthA and therefore could be important for the development of the canker lesions. Among the citrus proteins identified, we selected for studies a CsARF (Auxin Response Factor) and a CsHMG (High-mobility group), both involved in regulation of gene transcription. CsARF shares high similarity to the Arabidopsis thaliana ARF2, involved in the auxin signaling pathway. This is in line with our previous studies showing that auxin is required for canker development. The interactions between all variants of PthA were analyzed both in vivoand in vitro and depend on the repeat domain of PthAs. The B3 DNA binding and the Aux/IAA domains of CsARF are both involved in protein-protein interactions. Interestingly, the citrus promoter of a citrus expansin gene that is up-regulated by Xac and auxin contains putative CsARF and PthA binding sites. Since these sites are located adjacent in this promoter, it is suggested that the interaction of PthA with CsARF might somehow affect the regulation of the expansin promoter. CsHMG is highly similar to the A. thaliana HMGB1 involved in cell growth. CsHMG interacts with all PthA variants and its interaction was shown to be mediated primarly by the leucine-rich repeat (LRR) region of PthAs. CsHMG binds to DNA in a non-specific fashion; surprisingly, however, CsHMG shows an as yet unreported ability to bind to synthetic RNA forms with an apparent specificity to poly-U probes. PthA acts like an eukaryotic transcription factor and is not surprising that host proteins involved with gene regulation can interact with this effector, suggesting a new mode of action of these bacterial effector proteins. / Mestrado / Genetica Vegetal e Melhoramento / Mestre em Genética e Biologia Molecular

No description available.

No description available.

Actuellement le polyéthylènimine (PEI) est l’agent de transfection transitoire le plus utilisé par l’industrie pharmaceutique pour la production de protéines recombinantes à grande échelle par les cellules de mammifères. Il permet la condensation de l’ADN plasmidique (ADNp) en formant spontanément des nanoparticules positives appelées polyplexes, lui procurant la possibilité de s’attacher sur la membrane cellulaire afin d’être internalisé, ainsi qu’une protection face aux nucléases intracellulaires. Cependant, alors que les polyplexes s’attachent sur la quasi-totalité des cellules seulement 5 à 10 % de l’ADNp internalisé atteint leur noyau, ce qui indique que la majorité des polyplexes ne participent pas à l’expression du transgène. Ceci contraste avec l’efficacité des vecteurs viraux où une seule particule virale par cellule peut être suffisante. Les virus ont évolués afin d’exploiter les voies d’internalisation et de routage cellulaire pour exprimer efficacement leur matériel génétique. Nous avons donc supposé que l’exploitation des voies d’internalisation et de routage cellulaire d’un récepteur pourrait, de façon similaire à plusieurs virus, permettre d’optimiser le processus de transfection en réduisant les quantités d’ADNp et d’agent de transfection nécessaires. Une alternative au PEI pour transfecter les cellules de mammifèreest l’utilisation de protéines possédant un domaine de liaison à l’ADNp. Toutefois, leur utilisation reste marginale à cause de la grande quantité requise pour atteindre l’expression du transgène. Dans cette étude, nous avons utilisé le système E/Kcoil afin de cibler un récepteur membranaire dans le but de délivrer l’ADNp dans des cellules de mammifères. Le Ecoil et le Kcoil sont des heptapeptides répétés qui peuvent interagir ensemble avec une grande affinité et spécificité afin de former des structures coiled-coil. Nous avons fusionné le Ecoil avec des protéines capables d’interagir avec l’ADNp et le Kcoil avec un récepteur membranaire que nous avons surexprimé dans les cellules HEK293 de manière stable. Nous avons découvert que la réduction de la sulfatation de la surface cellulaire permettait l’attachement ciblé sur les cellules par l’intermédiaire du système E/Kcoil. Nous démontrons dans cette étude comment utiliser le système E/Kcoil et une protéine interagissant avec l’ADNp pour délivrer un transgène de manière ciblée. Cette nouvelle méthode de transfection permet de réduire les quantités de protéines nécessaires pour l’expression du transgène. / Pharmaceutical industry often employs polyethylenimine (PEI) for large scale protein production processes by transient transfection of mammalian cells. PEI condenses plasmid DNA (pDNA) by spontaneously forming positive nanoparticles known as polyplexes. Condensed pDNA is favoured for cell surface binding, internalization and protection from intracellular nucleases. While most of the cells efficiently uptake polyplexes, only 5 to 10% of captured pDNA reaches the nucleus for transgene expression. This suggests that polyplexes are hampered in their ability to route and to translocate to the nucleus necessitating large amounts of polyplexes to achieve high expression levels. By contrast, many viruses can efficiently transduce cells with only one or a few viral genome copies. Viruses have evolved to exploit cellular internalization and routing properties to express their own genetic material. We hypothesized that less pDNA would be used in an optimized transfection process if we exploited the internalization and routing properties that viruses use. DNA binding proteins could be used as an alternative to PEI to transfect mammalian cells. However, their usage is marginal due to the large protein quantities required to bind pDNA for transgene expression. If less pDNA is used less binding protein is needed. In this study, we used the E/Kcoil system to target a membrane receptor to deliver pDNA in mammalian cells. The Ecoil and Kcoil are two repeated heptapeptides which interact with a high affinity and specificity to form coiled-coil structures. We fused the Ecoil with a recombinant pDNA-binding protein. The Kcoil was fused to a stably-expressed membrane receptor in HEK293 cells. We discovered that low sulfation of the cell surface reduced non-specific binding of the pDNA:protein complex and permitted targeted binding via the E/Kcoil interaction. We demonstrate how to use recombinant pDNA-binding protein and the E/Kcoil system for targeted transgene delivery. This newly developed system provides a new transfection method, with reduced pDNA-binding protein quantities needed to achieve transgene expression.

La poly(ADP-ribosyl)ation est une modification post-traductionnelle des protéines catalysée par une famille d’enzymes : les poly(ADP-ribose) polymérases. Parmi les plus étudiées, PARP-1 et PARP-2 interviennent dans l’organisation, l’expression et le maintien de l’intégrité du génome. Nous avons initié l'étude d'un nouveau partenaire de PARP-1 préalablement identifié par double-hybride, et encore peu étudié à ce jour : HMG2L1 (High-Mobility Group protein 2 Like-1). La protéine humaine de 601 acides aminés contient un domaine HMG (High-Mobility Group) normalement impliqué dans l’interaction avec l’ADN. Quelques études ont montré que HMG2L1 régule la transcription en agissant comme co-régulateur négatif ou positif. Dans un premier temps, nous avons caractérisé le lien entre PARP-1 et HMG2L1. L’interaction avec PARP-1 a été confirmée in-vivo et in vitro. Nous avons montré que HMG2L1 pouvait également interagir avec PARP-2. HMG2L1 est poly(ADP-ribosyl)ée par PARP-1 et PARP-2, de même qu’elle est capable d’interagir avec le poly(ADP-ribose). La construction de formes tronquées de HMG2L1 en fusion avec la GFP nous a permis de montrer que le domaine N-terminal – en amont du domaine HMG – est impliqué dans ces interactions. Ce domaine N-terminal est très électropositif et intrinsèquement désordonné ce qui lui confère de nombreuses potentialités d’interactions. L’expression des fusions GFP dans des cellules HeLa nous a permis de montrer la localisation nucléaire et nucléolaire de HMG2L1, comme c’est le cas pour PARP-1 et PARP- 2. En outre, HMG2L1 colocalise avec UBF (Upstream Binding Factor), le facteur de transcription de l’ARN polymérase I responsable de la transcription des ARN ribosomaux. La surexpression de GFP-hHMG2L1 entraîne un stress nucléolaire caractérisé par l’inhibition de la transcription des ADNr et la formation de coiffes nucléolaires. Nous avons également entrepris une recherche de partenaires de HMG2L1 par spectrométrie de masse. De nombreuses protéines nucléolaires, impliquées dans la biogenèse des ribosomes ou la maturation des ARNs ont été identifiées, suggérant un rôle de HMG2L1 dans ces processus. Nous avons montré que la protéine purifiée interagit avec l’ADN via son domaine HMG principalement, et qu’elle interagit avec l’ARN via son domaine N-terminal. Mais surtout, nous avons mis en évidence une activité ARN-chaperonne, qui peut être régulée par le poly(ADP-ribose). La localisation de HMG2L1, son réseau d’interaction ainsi que son activité chaperonne nous laissent à penser qu’elle pourrait être impliquée dans des processus de maturation des ARN, régulés par la poly(ADPribosyl)ation. / Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated by a family of enzymes called poly(ADP-ribose) polymerases. Among the best studied, PARP-1 and PARP-2 are both implicated into the transcription, organization and integrity of genome. We have initiated the characterization of a new PARP-1 partner previously identified in a yeast two-hybrid screen, and still poorly studied: HMG2L1 (High-Mobility Group protein 2 Like-1). The human protein of 601 amino acids contains one HMGbox domain normally implicated in the recognition of DNA. Some studies have reported the role of HMG2L1 in the regulation of transcription by acting as a negative or positive coregulator. First, we characterized the link between PARP-1 and HMG2L1. We confirmed the interaction between both proteins in vivo and in vitro. We also showed that HMG2L1 couldinteract with PARP-2. HMG2L1 is poly(ADP-ribosyl)ated by PARP-1 and PARP-2, and is able to interact with poly(ADP-ribose). The construction of GFP-fused truncated versions of HMG2L1 allowed us to show that the N-terminal part – upstream to the HMGbox – is responsible for all these interactions. This N-terminal domain is highly electropositive and intrinsically disordered conferring a lot of interactions potentialities. The expression of the GFP-fused proteins in HeLa cells allowed us to localizeHMG2L1 into the nucleus and the nucleolus, like PARP-1 and PARP-2. Moreover, HMG2L1 colocalizes with UBF (Upstream Binding Factor), the transcription factor responsible for the transcription of ribosomal ARNs by RNA polymerase I. The overexpression of GFPhHMG2L1 leads to a nucleolar stress illustrated by the inhibition of transcription and the formation of nucleolar caps. We also undertook a proteomic study to find new partners of HMG2L1. We found a huge amount of nucleolar proteins, involved in ribosome biogenesis or RNA maturation, suggesting that HMG2L1 could be involved in these processes. Finally, we demonstrated the ability of the purified protein to interact with DNA mostly through its HMGbox domain and RNA through its N-terminal domain. Moreover, we discovered that HMG2L1 is endowed with a RNA-chaperone activity, that can be regulated by poly(ADP-ribose). Taken together, the localization of HMG2L1, its interacting partners and its RNA chaperone activity allow us to make the assumption that HMG2L1 could be implicated in RNA maturation processes, regulated by poly(ADP-ribosyl)ation.

Les principaux objectifs des opérateurs ferroviaires visent à accroître la sécurité, réduire les coûts d’exploitation et de maintenance et augmenter l’attractivité et les bénéfices du transport ferroviaire en offrant de nouveaux services aux passagers. Ceci ne pourra être atteint que grâce à la multiplication des échanges de données entre les différents acteurs du monde ferroviaire. L’interopérabilité, l’efficacité spectrale, l’optimisation de l’usage des ressources radio et l’amélioration de la fiabilité des communications sont des exigences fortes pour les applications de télécommunication ferroviaires. Les recherches dans le domaine de la radio cognitive ont vu le jour afin de répondre aux besoins de communication de l’armée ainsi qu’aux besoins dans les secteurs de la sécurité publique. Ces domaines partagent souvent les mêmes exigences que les chemins de fers. Ainsi, la radio cognitive a montré un potentiel prometteur pour répondre aux besoins listés précédemment. Une des principales fonctionnalités d’un dispositif de radio cognitive est de prendre conscience de son environnement radioélectrique et de détecter les bandes disponibles. Trois principaux éléments définissent l’environnement de la radio cognitive : l’utilisateur, les règles d’accès au spectre radio et les domaines radio. Cette thèse met en avant plusieurs contributions relatives à la reconnaissance de l’environnement radiofréquence et la détection de bandes libres. Plus spécifiquement, ces contributions portent sur la reconnaissance par la radio cognitive de l’occupation du spectre et de la modulation des signaux présents dans les bandes analysées. Ces fonctions ont été conçues pour le contexte ferroviaire, c’est-à-dire la grande vitesse et un environnement électromagnétique difficile en présence de bruit impulsif. / An essential goal of railway operators is to increase safety, reduce operation and maintenance costs, and increase attraction and profit by offering new services to passengers. These objectives will be reached thanks to a huge increase of data fluxes exchanges between railways stakeholders and infrastructures.Interoperability, spectral efficiency, optimization of radio resource usages, and improvement of communications reliability are of significant interest for railway applications. The Cognitive Radio (CR) research has been successfully applied to meet the communication needs of the military as well as the public-safety sectors, which share many of the same needs as railway. CRs have shown significant promise to answer all of the previously listed requirements. One of the main capabilities of a CR device is to sense and finally become aware of its environment. Three major domains define the environment of the CR, namely, the user, policy, and radio domains. This thesis highlights several contributions to radio environment awareness of a CR device. More specifically, these contributions lie in the spectrum awareness and waveform awareness functions of the CR. We designed these functions for the railways context, that is, a high speed vehicular context, besides difficult electromagnetic environments resulting a heavy-tailed impulsive noise.

Abstract This thesis concentrates on evaluating and improving the throughput performances of mobile users in high speed vehicles. In particular, high speed train (HST) scenarios are considered. Emphasis is placed on practical designs and methods that take into account distinctive HST characteristics. A two-hop communication link, i.e., base station (BS)-to-HST and HST-to-onboard users (OBUs) is adopted. The main target is to improve the throughput performance on the BS-to-HST communication link, which is assumed to be the main bottleneck in the whole communication link, since the HST-to-OBU communication link is assumed to have good channel quality due to the short link distance with relatively stationary OBUs. The algorithms developed are assessed through link and system level simulations. A theoretical and practical study of the throughput maximization problem in a single and multi-cell multiple-input multiple-output orthogonal frequency-division multiplexing (MIMO-OFDM) train scenario are considered with and without cooperation between train carriages. Two low-complexity transmission schemes based on simple antenna selection (AS) methods with spatial multiplexing (SM) are proposed. The simulation results demonstrate that large antenna arrays with AS and SM transmission strategies have the potential to significantly improve the throughput of the BS-to-train link in HST scenarios. Resource sharing methodologies between the moving relay nodes (MRNs) on the HST and ground macro users (GMUs) were also studied in a multi-cell MIMO-OFDM train scenario. Direct application of existing resource scheduling methods will not be appropriate to efficiently and fairly share resources, since the MRNs and the GMUs have different processing capabilities. Hence, two hybrid resource scheduling methods are analyzed in conjunction with joint and disjoint resource management. The tradeoff between the number of MRNs and receive antennas that should be installed on an HST was also examined in the context of throughput performance and capital expenditure. Results show that joint scheduling does not provide the best overall performance and there is a need to schedule each group of mobile terminals (MTs) separately. Finally, the feasibility of the use of higher frequency bands (HFBs) was examined in HST scenarios. A timer-based beam selection scheme for HST, which does not require any training time to select the appropriate beam is also proposed. The proposed beam selection scheme (PBSS) displays a close performance to the ideal singular value decomposition (SVD) scheme. / Tiivistelmä Tämä väitöskirja keskittyy mobiilikäyttäjien tiedonsiirtonopeuksien arviointiin ja parantamiseen nopeasti liikkuvissa kulkuneuvoissa. Työ käsittelee erityisesti tiedonsiirtoa suurnopeusjunissa. Työssä korostetaan käytännön menetelmiä, jotka ottavat huomioon nopeasti liikkuvien junien tiedonsiirron erityispiirteet. Työssä käytetään kahden hypyn linkkimallia, jossa tiedonsiirto kulkee tukiasemalta junaan ja junasta käyttäjälle, joka on junassa. Päätavoite on parantaa datanopeuksia tukiaseman ja junan välisessä tiedonsiirtolinkissä, jonka uskotaan olevan suurin pullonkaula koko tiedonsiirtolinkissä, koska junan ja lähes paikallaan olevan käyttäjän välinen kanava voidaan olettaa hyvälaatuiseksi linkin lyhyyden vuoksi. Kehitettyjen algoritmien suorituskykyä arvioidaan linkki- ja järjestelmätason simulaatioilla. Työssä tutkitaan tiedonsiirtonopeuden maksimointiongelmaa teoreettisella ja käytännön tasolla yhden ja usean solun MIMO OFDM junaskenaarioissa, joissa junan vaunut tekevät tai eivät tee yhteistyötä. Työssä esitetään kaksi alhaisen kompleksisuuden lähetysmenetelmää, jotka hyödyntävät yksinkertaista antennin valintamenetelmää ja tilatason multipleksointia. Simulointitulokset osoittavat, että suuret antenniryhmät, jotka hyödyntävät näitä lähetysmenetelmiä, voivat parantaa merkittävästi tiedonsiirtonopeutta tukiasemalta junaan päin. Työssä tutkitaan myös resurssien jakomenetelmiä liikkuvien junassa olevien releiden ja maatason makrokäyttäjien välillä monen solun MIMO-OFDM junaskenaariossa. Nykyisten resurssinhallintamenetelmien käyttö ei ole suoraan mahdollista tehokasta ja oikeudenmukaista resurssien jakoa, koska releillä ja makrokäyttäjillä on erilaiset prosessointikyvyt. Tämän vuoksi työssä analysoidaan kahta hybridimenetelmään resurssien skeduloinnille. Tutkimukset selventävät tasapainoa releiden lukumäärän ja junaan asennettavien vastaanotinantennien välillä tiedonsiirtonopeuden ja kustannusten osalta. Tulokset osoittavat, että yhteinen resurssien jako ei saavuta parasta suorituskykyä, eikä ole tarvetta ajoittaa jokaista matkaviestinterminaaliryhmää erikseen. Lopuksi työssä tutkitaan korkeampien taajuusalueiden soveltuvuutta tiedonsiirtoon suurnopeusjunissa. Työssä ehdotetaan ajastinpohjaista keilanvalintamenetelmää, joka ei vaadi opetusjaksoa sopivan keilan valintaan. Ehdotetun menetelmän saavuttama suorituskyky on lähellä ideaalisen singulaariarvohajotelmaa hyödyntävän menetelmän suorituskykyä.

Mature αβ and γδ T cells arise from a common precursor population in the thymus. Much debate has focused on the mechanism of T cell lineage choice made by these multi-potential precursor cells. It is widely believed that the decision of these precursor cells to commit to the γδ or αβ T cell lineages is regulated primarily by a specific instructive signal relayed through the appropriate T cell receptor. Contrary to this model, we present evidence for a TCR-independent lineage commitment process. Comparison of global gene expression profiles from immature αβ and γδ lineage thymocytes identified Sox13, an HMG-box transcription factor, as a γδ T cell-specific gene. Unlike other HMG-box transcription factors such as TCF1, LEF1 and SOX4, that are critical for proper αβ T cell development, Sox13 expression is restricted to early precursor subsets and γδ lineage cells. Importantly, SOX13 appears to influence the developmental fate of T cell precursors prior to T cell receptor expression on the cell surface. Transgenic over-expression of Sox13 in early T cell precursors strongly inhibits αβ lineage development, in part, by inhibiting precursor cell proliferation and concomitantly, leading to increased cell death among αβ lineage subsets. Steady-state γδ T cell numbers, however, appear unaffected. Strikingly, the DP αβ lineage cells that do develop in Sox13 transgenic mice are imprinted with a γδ- or precursor-like molecular profile, suggesting that SOX13 plays an active role in the lineage fate decision process or maintenance. Sox13-deficient mice, on the other hand, have selectively reduced numbers of γδ thymocytes, indicating that SOX13 is essential for proper development of γδ T cells. We present additional data demonstrating that SOX13 is a canonical WNT signaling antagonist modulating TCF1 activity, raising a strong possibility that WNT signals, and their modulators, are at the nexus of γδ versus αβ T cell lineage commitment.