Ingestion of proteins with high leucine content during resistance training (RT) can augment hypertrophy. There are data suggesting that a leucine metabolite, β-hydroxy, β-methylbutyrate (HMB), may, however, be substantially more anabolic than leucine. Purpose: We aimed to test whether supplementation with HMB versus leucine, added to whey protein, would result in different muscle hypertrophy and strength gains in young men performing resistance training (RT). Methods: Twenty-six resistance-trained men (23 ± 2 y) performed 12 wk of RT with 3 phases. Phase 1: 8 wk of periodized RT (3 training sessions/wk). Phase 2: 2 wk overreaching period (5 sessions/wk). Phase 3: 2 wk taper (3 sessions/wk). Participants were randomly assigned to twice daily ingestion of: whey protein (25 g) plus HMB (1.5 g) (Whey+HMB; n=13) or whey protein (25 g) plus leucine (1.5 g) (Whey+Leu; n=13). Skeletal muscle biopsies were performed before and after RT. Measures of fat and bone-free mass (FBFM), vastus lateralis (VL) muscle thickness and muscle cross-sectional area (CSA – both by ultrasound), muscle fiber CSA, and 1-repetition maximum (1-RM) strength tests were determined. Results: We observed increases in FBFM, VL muscle thickness, muscle CSA and fiber type CSA and 1-RM strength, with no differences between HMB and leucine at any phase. Furthermore, no differences were observed in hormone concentrations between groups, or in time-by-group interactions in hormone concentrations at any phase of the RT program. Conclusion: HMB did not result in greater increases in any measure of muscle mass, strength, or hormonal concentration compared to leucine during 12 weeks of RT. / Thesis / Master of Science in Kinesiology / Whey protein supplementation following resistance training (RT) is an effective strategy to enhance RT-induced gains in skeletal muscle mass and strength. The anabolic properties of whey protein are attributed, in part, to the branched-chain amino acid leucine. Leucine is a substrate for protein synthesis and a potent signal that turns on the protein synthetic machinery. A metabolite of leucine, β-hydroxy, β-methylbutyrate (HMB) has been claimed to share similar or greater anabolic properties of leucine. Recently, supplementation with HMB during RT has been shown to result in large gains in muscle mass and strength. The purpose of this study was to examine whether HMB, versus leucine, added to whey protein, would result in different muscle hypertrophy and strength gains in young men during RT. Body composition and maximum strength tests were performed before, during and after 12 weeks of RT. Following 12 weeks of RT, both groups experienced similar gains in muscle mass and strength. We observed that HMB is no more effective in stimulating RT-induced hypertrophy and strength gains than its parent amino acid, leucine.

Physically active men and woman may be less likely than their inactive peers to become overweight. Exercise has a favourable effect on body fat distribution, with a reduction in waist-to-hip ratio with increased exercise. Exercise is especially important in maintaining weight loss in overweight individuals. Physical activity can directly affect both total energy intake and total energy expenditure. Physical activity can also affect fat balance and it is becoming clear that imbalances in total energy are largely due to imbalances in the distribution of fat. Exercise also has additional, beneficial effects on most of the metabolic risk factors for cardiovascular disease and non-insulin dependent diabetes mellitus. Exercise testing provides a basis for the design of training programs and allows for monitoring progress throughout the training program. Used properly, testing and monitoring is useful to both trainers and subjects. Therefore, exercise in conjunction with an appropriate diet is beneficial to overweight individuals and provided that feasible methods and motivation are available, we recommend exercise as an important part of a weight control program. The aim of this study was to determine whether HMB supplementation will increase the Lean Body Mass (LBM) and muscle power output (measured as the load a subject can bench press) of males who gym for recreational purposes, after a combination of resistance weight training, eating a balanced set diet and supplementation with HMB for 8 weeks. Two homogenous groups of 20 males were evaluated for initial strength capabilities and body composition. For 8 weeks the subjects lifted weights three times a week and followed a balanced diet. Creatinekinase activity decreased with HMB supplementation. Gains in muscle power output were greater in the experimental group, and fat percentage decreases were recorded with HMB supplementation. / Dissertation (MSc)--University of Pretoria, 2011. / Physiology / unrestricted

Atualmente está aumentando o número de pessoas que usam suplementos nutricionais com a finalidade de obter melhores resultados nas suas atividades esportivas, perda de gordura, ganhos de massa muscular, ou na plasticidade do corpo. Tal fato merece atenção porque muito pouco é conhecido sobre a segurança ou eficácia destes produtos, e apenas 14% dos usuários buscam orientações com profissional da saúde sobre o uso destas substâncias. Dos mais de 200 suplementos que prometem estes efeitos apenas as suplementações de creatina HMB produzem os resultados prometidos, e o Comitê Olímpico qualifica o uso do HMB como legal. Embora a literatura atual mostre os benefícios no uso da suplementação com HMB, são poucas as informações sobre o seu efeito na morfofisiologia das fibras musculares como, por exemplo, o perfil histoenzimológico e a área dos diferentes tipos de fibras musculares. Baseado nestas informações pensou-se na realização desde trabalho para verificar se o uso de HMB provoca alterações morfológicas e histoenzimológicas nas fibras musculares dos músculos da mastigação; se estes efeitos alterariam o desenvolvimento e crescimento do esqueleto craniofacial; e se os efeitos sobre os componentes do sistema estomatognático seriam semelhantes nos indivíduos ambos os gêneros. Para realização deste estudo foram utilizados 58 ratos com idade de 60 dias, 29 animais de cada gênero, distribuídos nos seguintes grupos: Grupo Controle Inicial (GCI) que foram sacrificados no inicio do experimento; Grupo Controle Placebo (GCP) que receberam o mesmo volume do veículo do grupo experimental, e alimentação ad libitum; Grupo Experimental (GE) que receberam diariamente 0,3g/kg de HMB, por meio de gavagem e mesma quantidade de alimentos que GCP consumiu no dia anterior; Grupo Experimental Ad libitum (GEA) que receberam a mesma dose da droga, porém tiveram alimentação ad libitum. Após o tratamento, foram retiradas amostras dos músculos digástrico (ventre anterior) e masseter (porção superficial), para análise histoenzimologica (m-ATPAse com pré-incubações ácida e alcalina) e retirada do esqueleto cefálico para tomadas das medidas craniométricas. Os dados obtidos foram submetidos a tratamento estatístico. Os resultados permitiram concluir que: o uso diário de 0,3g/kg de HMB, por quatro semanas: a) não provocou alterações morfológicas e histoenzimológicas nas fibras muscular dos músculos da mastigação; b) os resultados foram semelhantes entre os músculos elevadores e abaixadores da mandíbula; c) não provoca alterações no desenvolvimento e crescimento do esqueleto craniofacial; d) a falta de resultados positivos de alterações nos músculos da mastigação e crescimento craniofacial, sugere existiram um relação entre eles; e) os resultados sobre os componentes do sistema estomatognático foram semelhantes nos indivíduos ambos os gêneros. / Nowadays is increasing the number of people who use nutritional supplements in order to achieve better results in their sports, fat loss, muscle gains, or the plasticity of the body. This deserves attention because very little is known about the safety or efficacy of such products, and only 14% of users seeking guidance with a health professional regarding the use of these substances. Of the more than 200 supplements that promise these effects only the HMB and creatine supplementation produce the promised effects, and the Olympic Committee qualifies the use of HMB as legal. While the literature shows benefits in the use of HMB supplementation, there is little information on its effect on muscle fibers morphophysiology as, for example, the profile and the area histoenzimológico of different types of muscle fibers. Based on this information, it was thought in performing this work to check whether the use of HMB causes morphological and histoenzimológicas changes in muscle fibers of the muscles of mastication, these effects alter the growth and development of craniofacial skeleton, and if the effects on the components of stomatognathic system would be similar in both genders individuals. For this study we used 58 rats aged 60 days, 29 animals of each gender, divided into four groups: Control Group Home (GCI) which were sacrificed at the beginning of the experiment; Placebo Control Group (GCP) that received the same volume Vehicle experimental group, and fed ad libitum; Experimental Group (EG) which received daily 0.3 g / kg of HMB, by gavage and the same amount of food they consumed on the previous day GCP; experimental group ad libitum (GEA) who received the same dose of the drug, but were fed ad libitum. After treatment, samples were taken of the digastric (anterior belly) and masseter (superficial part) for analysis histoenzimologic (m-ATPase with pre-incubations acid and alkaline) and removal of the head skeleton to taken the craniometric measures. The results showed that: the daily use of 0.3 g / kg of HMB for four weeks: a) caused no morphological changes and histoenzimológicas muscle fibers in the muscles of mastication; b) the results were similar between muscles elevators and depressors of the jaw; c) does not cause changes in the development and growth of the craniofacial skeleton; d) the lack of positive changes in the masticatory muscles and craniofacial growth, suggests a relationship existed between them, e) results the components of the stomatognathic system were similar in subjects both genders.

Atualmente está aumentando o número de pessoas que usam suplementos nutricionais com a finalidade de obter melhores resultados nas suas atividades esportivas, perda de gordura, ganhos de massa muscular, ou na plasticidade do corpo. Tal fato merece atenção porque muito pouco é conhecido sobre a segurança ou eficácia destes produtos, e apenas 14% dos usuários buscam orientações com profissional da saúde sobre o uso destas substâncias. Dos mais de 200 suplementos que prometem estes efeitos apenas as suplementações de creatina HMB produzem os resultados prometidos, e o Comitê Olímpico qualifica o uso do HMB como legal. Embora a literatura atual mostre os benefícios no uso da suplementação com HMB, são poucas as informações sobre o seu efeito na morfofisiologia das fibras musculares como, por exemplo, o perfil histoenzimológico e a área dos diferentes tipos de fibras musculares. Baseado nestas informações pensou-se na realização desde trabalho para verificar se o uso de HMB provoca alterações morfológicas e histoenzimológicas nas fibras musculares dos músculos da mastigação; se estes efeitos alterariam o desenvolvimento e crescimento do esqueleto craniofacial; e se os efeitos sobre os componentes do sistema estomatognático seriam semelhantes nos indivíduos ambos os gêneros. Para realização deste estudo foram utilizados 58 ratos com idade de 60 dias, 29 animais de cada gênero, distribuídos nos seguintes grupos: Grupo Controle Inicial (GCI) que foram sacrificados no inicio do experimento; Grupo Controle Placebo (GCP) que receberam o mesmo volume do veículo do grupo experimental, e alimentação ad libitum; Grupo Experimental (GE) que receberam diariamente 0,3g/kg de HMB, por meio de gavagem e mesma quantidade de alimentos que GCP consumiu no dia anterior; Grupo Experimental Ad libitum (GEA) que receberam a mesma dose da droga, porém tiveram alimentação ad libitum. Após o tratamento, foram retiradas amostras dos músculos digástrico (ventre anterior) e masseter (porção superficial), para análise histoenzimologica (m-ATPAse com pré-incubações ácida e alcalina) e retirada do esqueleto cefálico para tomadas das medidas craniométricas. Os dados obtidos foram submetidos a tratamento estatístico. Os resultados permitiram concluir que: o uso diário de 0,3g/kg de HMB, por quatro semanas: a) não provocou alterações morfológicas e histoenzimológicas nas fibras muscular dos músculos da mastigação; b) os resultados foram semelhantes entre os músculos elevadores e abaixadores da mandíbula; c) não provoca alterações no desenvolvimento e crescimento do esqueleto craniofacial; d) a falta de resultados positivos de alterações nos músculos da mastigação e crescimento craniofacial, sugere existiram um relação entre eles; e) os resultados sobre os componentes do sistema estomatognático foram semelhantes nos indivíduos ambos os gêneros. / Nowadays is increasing the number of people who use nutritional supplements in order to achieve better results in their sports, fat loss, muscle gains, or the plasticity of the body. This deserves attention because very little is known about the safety or efficacy of such products, and only 14% of users seeking guidance with a health professional regarding the use of these substances. Of the more than 200 supplements that promise these effects only the HMB and creatine supplementation produce the promised effects, and the Olympic Committee qualifies the use of HMB as legal. While the literature shows benefits in the use of HMB supplementation, there is little information on its effect on muscle fibers morphophysiology as, for example, the profile and the area histoenzimológico of different types of muscle fibers. Based on this information, it was thought in performing this work to check whether the use of HMB causes morphological and histoenzimológicas changes in muscle fibers of the muscles of mastication, these effects alter the growth and development of craniofacial skeleton, and if the effects on the components of stomatognathic system would be similar in both genders individuals. For this study we used 58 rats aged 60 days, 29 animals of each gender, divided into four groups: Control Group Home (GCI) which were sacrificed at the beginning of the experiment; Placebo Control Group (GCP) that received the same volume Vehicle experimental group, and fed ad libitum; Experimental Group (EG) which received daily 0.3 g / kg of HMB, by gavage and the same amount of food they consumed on the previous day GCP; experimental group ad libitum (GEA) who received the same dose of the drug, but were fed ad libitum. After treatment, samples were taken of the digastric (anterior belly) and masseter (superficial part) for analysis histoenzimologic (m-ATPase with pre-incubations acid and alkaline) and removal of the head skeleton to taken the craniometric measures. The results showed that: the daily use of 0.3 g / kg of HMB for four weeks: a) caused no morphological changes and histoenzimológicas muscle fibers in the muscles of mastication; b) the results were similar between muscles elevators and depressors of the jaw; c) does not cause changes in the development and growth of the craniofacial skeleton; d) the lack of positive changes in the masticatory muscles and craniofacial growth, suggests a relationship existed between them, e) results the components of the stomatognathic system were similar in subjects both genders.

Carbohydrate plus additional protein supplementation provided immediately after exercise has been found to increase the rate of muscle glycogen restoration compared to carbohydrate alone. To examine whether leucine, and/or β-hydroxy-β-methylbutyrate (HMB) to carbohydrate plus protein supplementation affects short-term recovery (45 min) of muscle glycogen, we compared plasma glucose and insulin, the muscle glycogen concentration, and the cellular signaling proteins controlling muscle glycogen synthesis 45 min after supplementation. Rats (n=35) underwent high-intensity resistance exercise followed by supplementation with carbohydrate (CHO: 1.2g/kg body weight), carbohydrate with whey protein (CP: 1.2g CHO + 375mg whey protein/kg body weight), carbohydrate with whey protein plus HMB (CPH: 1.2g CHO + 375mg whey protein + 400mg HMB/kg body weight), carbohydrate with whey protein, HMB plus leucine (CPHL: 1.2g CHO + 375mg whey protein + 400mg HMB + 444mg leucine/kg body weight) or exercise only (CON). Blood samples were collected immediately after exercise and 45 min after supplementations. Muscle samples of plantaris were excised immediately and 45 min post-exercise. Plasma glucose was increased by CHO and CPH supplementation and reduced by CPHL at 45 min post-exercise. Plasma insulin was elevated by CP and CPHL treatments compare to CHO. Muscle glycogen concentration was unaffected by all treatments and did not differ from CON. Phosphorylation of Akt/PKB, GSK3α/β, and GS at 45 min of recovery for all supplements was not significant difference from CON. Phosphorylation of mTOR was significantly increased by CPHL and CP supplementation compared to CON, CHO, and CPH. Phosphorylation of AS160 was markedly reduced by CPH supplementation compared to CON. These results suggest that supplementing with carbohydrate plus protein with or without leucine and its metabolite, HMB, to enhance muscle glycogen replenishment following exercise may not provide an advantage during the early phase of recovery (45 min). Furthermore, there is some indication that HMB may elicit insulin resistance, and this needs further evaluation. / text

No description available.

För att diagnostisera malignt melanom och dess undergrupper används immunhistokemi för att färga in celler som uttrycker specifika protein. Särskilt desmoplastiska melanom kan initialt felbedömas baserat på utseendet som ärr, och det är därför viktigt att ha sensitiva antikroppar för att diagnostisera dem. Elva arkiverade patientprover (varav tre epiteloida melanom, fyra spolcelliga, tre desmoplastiska och ett akralt lentiginöst) färgades in med antikroppar mot CK18, HMB-45, Melan-A, S100, SOX10 och synaptophysin. Alla prover var negativa för CK18, nio var positiva för HMB-45 och Melan-A (de negativa var båda desmoplastiska melanom) och alla var positiva för S100 och SOX10. Synaptophysin var positiv i alla epiteloida melanom och det akralt lentiginösa melanomet, två av de fyra spolcelliga melanomen och negativ i alla desmoplastiska melanom. Fördelarna med SOX10, som tidigare studier i ämnet har påvisat, observerades inte i denna studie, troligen på grund av begränsningen i patientmaterial. Trots det verkar SOX10 vara ett användbart tillägg i melanompanelen, eller kanske till och med kan ersätta S100, baserat på tidigare studier.

As muscle function deteriorates with age, the discovery of new ways to enhance the quality of human life by stunting inevitable aging processes, such as sarcopenia, is a subject of great interest to aging populations, to health care professionals and to nutritional companies. β-hydroxy-β-methylbutyrate (HMB) enhances muscle strength in humans and attenuates disease-induced and disuse-dependent atrophy in rodents. We investigated the feasibility of utilizing Drosophila as a model organism to study the biological effects of HMB in aging muscle. Using flight ability as an index of muscle function and monitoring the lifespan of flies, we found that normal food supplemented with 10 mg/mL HMB, supplied from eclosion through adulthood, attenuates the age-dependent decline in flight ability and enhances longevity of flies. To further discern the dietary optimization of HMB supplementation, we examined the effect of 10 mg/mL HMB supplementation from the larval stages throughout adulthood under various dietary conditions. As dietary restriction without malnutrition and HMB supplementation independently increase longevity in flies, we investigated the relationship between dietary restriction and HMB supplementation. Flies were subject to non-restricted, reduced yeast (protein), or intermittently cycled feeding regimens, with or without HMB. Both modes of dietary restriction improved flight ability, while only cycling flies on and off food increased lifespan and improved survivorship of the flies. HMB supplementation increased flight ability later in life in all groups, but had differential effects on lifespan; HMB improved the survivorship of females fed a reduced yeast diet, but decreased the survivorship of both non-restricted and intermittently fed flies. This not only suggests that HMB may act via different pathways to influence fly flight and survivorship, but that these mechanisms may differ under various dietary conditions, in different sexes. Because HMB supplemented dietary restriction had different effects on flight ability and survival than dietary restriction alone, HMB likely acts via different mechanisms than dietary restriction. Sex specific effects were found, suggesting that future HMB and dietary restriction studies should make distinctions between effects in males and females. As the flight ability and longevity of D. melanogaster was affected by HMB supplementation under various dietary conditions, future studies may use D. melanogaster as a model organism to explore the effects of HMB on age-related muscle deterioration and to help uncover the biological mechanisms of such observations, as well as potential treatments for age-associated muscle dysfunction and disease.

No description available.

No description available.