Thesis (Mnutr)--Stellenbosch University, 2003. / ENGLISH ABSTRACT: INTRODUCTION: The objective of this pilot study was to evaluate the effects of a new micronutrient, glutamine, pre- and probiotic enriched liquid nutritional supplement on the nutritional status and immunity of adults living with HIV/AIDS. The study was designed as a prospective randomised double-blind placebo-controlled trial. Subjects were HIV-infected male and female adult volunteers (n = 47) from a community-based hospice centre in a peri-urban area in a resource-poor setting and were included irrespective of duration or clinical stage of HIV/AIDS. None of the subjects received antiretroviral therapy. METHOD: The intervention involved the daily ingestion of 40g (200 ml reconstituted) of either the enriched test product or an lsocalorie carbohydrate placebo for a period of 12 weeks. Anthropometric assessment (weight, height and triceps skinfold thickness; mid-upper arm, waist and hip circumferences) was performed at baseline and thereafter every 4 weeks (4 times). Biochemical (serum total protein, serum albumin and C-reactive protein) and haematological (full blood count and immunophenotyping) assessment was performed at baseline and again after week 12. RESULTS: Statistical analysis of baseline values was performed with Wilcoxon two-sample tests for comparison between the supplemented and placebo groups. Outcomes were evaluated using analysis of variance with Shapiro-Wilk tests and thereafter either pair-wise t-tests or sign tests (for nonparametric data) were used. Thirty-two subjects completed the trial, 14 in the supplemented group and 18 in the placebo group. Weight increased significantly in the supplemented group (2.73 ± 3.53 kg, P = 0.013). Triceps skinfold thickness increased significantly in both the supplemented (p = 0.047) and placebo group (p = 0.001). No other significant anthropometric change was observed. Serum albumin increased significantly in the supplemented group (p = 0.003) and was associated with a significant decline in C-reactive protein (p = 0.028). Haemoglobin decreased significantly in both groups. A significant decline in CD4+ count was observed in the placebo group while the decline in the supplemented group did not reach significance. CONCLUSION: Oral nutritional supplementation in limited quantities was well tolerated for a period of 3 months. This study demonstrated that an enriched nutritional supplement was able to promote weight gain and ameliorate hypoalbuminaemia and possibly inflammation in adults living with HIV/AIDS in the short to medium term. The enriched nutritional supplement does not appear to have an effect on the immunity of people with HIV/AIDS. The small sample is a limitation of the study and the conclusions pertain to the test product as a whole and not to any of its respective ingredients. Although further studies are required to evaluate long-term feasibility, these findings suggest that the use of an enriched nutritional supplement has a role in the management of weight loss in persons with HIV/AIDS. / AFRIKAANSE OPSOMMING: INLEIDING: Die doel van hierdie loodsstudie was om die uitwerking van 'n nuwe mikronutriënt, glutamien, pre- en probiotika verrykte voedingsaanvulling in vloeistof vorm te ondersoek. Die studie is ontwerp as 'n prospektiewe ewekansige dubbelblinde plasebogekontroleerde toets. Proefpersone was MIV-geïnfekteerde manlike and vroulike vrywilligers (n = 47) van 'n gemeenskapsgebaseerde hospitium in a semi-stedelike gebied in 'n hulpbron-arme omgewing. Proefpersone is ingesluit ongeag die duur of kliniese graad van MIVNIGS. Geen proefpersoon het antiretrovirale behandeling ontvang nie. METODE: Die intervensie het die daaglikse inname van 40g (200 ml gerekonstitueer) van óf die toetsproduk óf 'n isokaloriese koolhidraatplasebo gedurende 'n 12 week periode behels. Antropometriese evaluering (gewig, lengte en trisepsvelvoudikte; midbo-arm-, middel- en heupomtrekke) is uitgevoer met aanvang en daarna weer elke 4 weke (4 keer). Biochemiese (serum totale protein, serumalbumien en C-reaktiewe protein) en hematologiese (volbloedtelling en immunofenotipering) evaluering is uitgevoer met aanvang en weer na 12 weke. RESULTATE: Statistiese verwerking van basislyndata is gedoen deur middel van Wilcoxon twee-steekproef toetse waarmee vergelyking tussen die aangevulde en plasebogroep uitgevoer is. Studiegevolge is geëvalueer deur verspeidingsanalise met behulp van Shapiro-Wilk toetse waarna óf paargewyse t-toetse óf tekentoetse (vir nie-parametriese data) gebruik is. Twee-en-dertig proefpersone het die studietydperk voltooi, 14 in die aangevulde groep en 18 in die plasebogroep. Gewig het betekenisvol toegeneem in die aangevulde groep (2.73 ± 3.53 kg, p = 0.013). Triseps velvoudikte het betekenisvol toegeneem in beide die aangevulde (p = 0.047) en die plasebogroep (p = 0.001). Geen ander betekenisvolle antropometriese veranderinge is waargeneem nie. Serumalbumien het betekenisvol gestyg in die aangevulde groep (p = 0.003) en het gepaard gegaan met 'n betekenisvolle daling in C-reaktiewe protein (p = 0.028). Hemoglobienwaardes het in beide groepe betekenisvol gedaal. 'n Betekenisvolle daling in CD4+ telling is waargeneem in die plasebogroep terwyl die daling in die aangevulde groep nie betekenisvol was nie. GEVOLGTREKKING: Mondelingse voedingsaanvulling van 'n beperkte hoeveelheid was goed aanvaar en verdra oor 'n 3-maande tydperk. Hierdie studie toon dat 'n verrykte voedingsaanvulling in staat is om gewigstoename te bevorder en om hipoalbumienemie en moontlik ook inflammasie te verlig in volwassenes met MIVNIGS oor 'n kort tot medium tydperk. Die verrykte voedingsaanvulling blyk nie 'n effek op die immuniteit van mense met MIVNIGS te hê nie. Die klein steekproef is 'n beperking van die studie en die gevolgtrekkinge is slegs van toepassing op die toetsproduk as 'n geheel en nie op enige van die onderskeie bestanddele daarvan nie. Hoewel verdere studies nodig geag word om langtermyn uitvoerbaarheid te ondersoek, dui hierdie bevindinge daarop dat die gebruik van 'n verrykte voedingsaanvulling 'n rol speel in die beheer van gewigverlies in persone met MIVNIGS.

Thesis (MNutr)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: Perceptions held by Masters Students of the NOMAa track module on ‘Nutrition, Human Rights and Governance’ Introduction and Objectives The interdisciplinary NOMA Track module on ‘Nutrition, Human Rights and Governance’ was developed jointly by four higher learning institutions in three countries (Norway, South Africa and Uganda). The module was incorporated into the respective Masters programmes in nutrition and consisted of three study units each of six weeks duration. The units were presented consecutively in the three countries and built on one another, totalling 18 weeks. Objectives The main aim of the study was to document perceptions of Masters students who completed the module. The objectives were to compare the perceptions of students about the study units in different countries, to document how students anticipated utilising their newly-acquired knowledge and to make recommendations for the development of similar interdisciplinary modules. Methodology A cross-sectional study design with a mixed methods approach was used. The sampling frame consisted of all registered students enrolled in the module. Informed written consent for both voluntary participation as well as voice recording of interviews was obtained from all participants. Data was extracted from students’ quantitative evaluation of each study unit. An interpretative methodological approach was used to elicit narrative accounts of students’ perceptions of the module during in-depth telephonic interviews. An inductive process was followed to identify emerging themes. The code list thus compiled was used to analyse unstructured data by using a text analysis computer programme. Results Twenty NOMA students enrolled at different universities participated in the study. The module was described as a life-changing experience and the way the module was structured and conducted in both developed and developing countries was highly rated. Interactive teaching styles optimised the learning experience. Presentations by a variety of experts and study visits served to enrich theoretical human rights principles by giving practical examples and by comparing implementation of these principles under varying conditions. The main difference between the study units in the different countries related to time-management and different teaching styles employed by lecturers. Transnational and interdisciplinary education provided this group of students the opportunity to enhance various professional attributes. Their willingness to learn from others provided them with valuable insight about the diverse nature of different population groups and their cultural differences. Without this understanding, poor communication, intolerance and prejudice might create barriers to optimal treatment or education of a client/community requiring professional advice. Students applied their newly acquired knowledge about human rights principles and the link with nutrition by utilising teaching opportunities, and indicated that they intended to incorporate a human rights approach in future endeavours. Conclusion The interdisciplinary NOMA Track module empowered a group of students to utilise the principles of a human rights-based approach in an appropriate manner. NOMA students developed an understanding of their new role as nutrition professionals, being challenged to interact in a globalized world if they want to make a meaningful contribution to the realisation of the right to food for all. Lessons learned from the implementation of the module will be useful to inform further decision-making on how to integrate a focus on human rights into training in nutrition at Stellenbosch University. / AFRIKAANSE OPSOMMING: Persepsies van Meesterstudente oor die NOMAb-opsie module oor ‘Voeding, Menseregte en Bestuur’ Inleiding en Doelwitte Die interdissiplinêre NOMA-module opsie oor ‘Voeding, Menseregte en Bestuur’ is gesamentlik ontwikkel deur vier hoër opleidingsinstansies in drie lande: Noorweë, Suid Afrika en Uganda. Die module is geïnkorporeer in die toepaslike meestersgraadkursusse in voeding. Dit het bestaan uit drie studie-eenhede wat elkeen 6 weke geduur het (in totaal 18 weke) en opeenvolgend aangebied is in die drie lande. Doelwitte Die hoofdoel van die studie was om persepsies van meesterstudente wat die module voltooi het te dokumenteer. Die doel was om sodanige persepsies oor die studie-eenhede in verskillende lande te vergelyk, om te dokumenteer op watter wyse studente verwag om die nuwe kennis te benut en om aanbevelings te maak vir die ontwikkeling van soortgelyke interdissiplinêre modules. Metode ‘n Dwarssnit studieontwerp met ‘n gemengde metode benadering is gevolg. Die steekproefraamwerk het bestaan uit alle geregistreerde studente wat ingeskryf het vir die module. Ingeligde, geskrewe toestemming vir vrywillige deelname asook om stemopnames van onderhoude te maak, is van alle deelnemers verkry. Data is onttrek uit studente se kwantitatiewe evaluering van elke studie-eenheid. ‘n Metodologiese benadering van interpretasie is gevolg om ‘n narratiewe weergawe van studente se persepsies te ontlok gedurende in-diepte telefoniese onderhoude. Temas is induktief geïdentifiseer en ‘n ooreenstemmende kodelys is gebruik om ongestruktureerde data te analiseer met ʼn teksanalise rekenaarprogram. Resultate Twintig NOMA studente, ingeskryf by verskillende universiteite, het deelgeneem aan die studie. Die module is beskryf as ʼn lewensveranderende ondervinding. Die wyse waarop die module gestruktureer en aangebied is in beide ontwikkelde en ontwikkelende lande, is hoog op prys gestel. Die interaktiewe onderrigstyl het die leerervaring optimaal bevorder. Aanbiedings deur verskeie kundiges en studiebesoeke het daartoe bygedra dat teoretiese menseregte-beginsels verbreed is deur praktiese voorbeelde te verskaf. Die implementering van hierdie beginsels onder verskillende omstandighede is vergelyk. Die grootste verskille tussen die studie-eenhede in die onderskeie lande het verband gehou met tydsbesteding en dosente se verskillende onderrigstyle. Transnasionale en interdissiplinêre onderrig het hierdie groep studente geleentheid gegun om verskeie professionele vaardighede te ontwikkel. Hul bereidheid om by ander te leer het waardevolle insae gegee in die diverse aard van verskillende populasiegroepe en kultuurverskille. Daarsonder kon struikelblokke ontstaan het weens swak kommunikasie, onverdraagsaamheid en vooroordele. Dit kan verhoed dat optimale behandeling of onderrig verskaf word aan ʼn kliënt/gemeenskap wat professionele advies benodig. Studente het hul nuut-verworwe kennis oor menseregte-beginsels en die verband met voeding toegepas in onderriggeleenthede en het onderneem om ‘n menseregte-benadering te volg in toekomstige ondernemings. Gevolgtrekking Die interdissiplinêre NOMA-module opsie het ʼn groep studente bemagtig om die beginsels van ʼn menseregte-benadering op ʼn toepaslike wyse te gebruik. NOMA studente verstaan nou hul nuwe rol as kundiges in voeding, naamlik dat hulle uitgedaag word tot interaksie in ʼn globaliserende wêreld, as hulle ʼn merkbare bydrae wil lewer tot die verwesenliking van die reg tot voldoende voedsel vir almal. Lesse wat geleer is uit die implementering van die module sal waardevol wees wanneer daar besluite geneem word oor die beste manier waarop ʼn menseregte fokus ingesluit kan word in voeding-opleiding by Stellenbosch Universiteit.

Thesis (Mnutr)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: Inappropriate feeding practices are a major cause of malnutrition in young children. Within this context, it has been well documented that the incidence of malnutrition rises sharply during the period from six to 18 months of age in most countries. Complementary feeding typically covers the period from six to 24 months of age. Renewed focus has been placed on the promotion of breastfeeding. Similar attention should be paid to complementary feeding. Six percent of deaths per year are preventable through good complementary feeding practises. To improve infant nutrition it is important to know the local infant and young child feeding practises present in communities but also to identify and understand the underlying factors that influence these practises. Aim The study aimed to describe the various factors that influence complementary feeding practices of infants aged 6 – 12 months in 2 communities (Avian Park and Zweletemba) in the Breede Valley district of the Western Cape. Methods The study was conducted from May – July 2012. A descriptive study design was used. A qualitative approach was followed with the use of focus group discussions with mothers / primary caregivers, fathers and grandmothers of infants aged 6 – 12 months. Results The findings of this study provide insight into different aspects regarding early cessation of breastfeeding that could lead to early introduction of complementary foods. In both Avian Park and Zweletemba the age of introduction of liquids and solids ranged from birth to 12 months. Various liquids such as water, over the counter medicine, high concentrated sugar beverages and low nutrient beverages were given to infants from as young as two days post-partum by means of a feeding bottle. Cow’s milk was also introduced before six months of age for reasons such as affordability, availability and because cow’s milk does not need boiling water for reconstitution like formula milk, especially when access to electricity is inadequate. Infants from both communities also received meelbol (flour and water beverage) fed either via feeding bottles (as a beverage) or as porridge fed to the infant with a spoon. Porridge (especially rice cereal and maize meal porridge) was introduced to infants from one week post-partum and infants from both areas also received family “food from the pot” before the age of 6 months. In this study it was found that it was most often a female (either the mother or the grandmother) in the household who was responsible for buying and preparing food and for feeding the infant. Health care workers, members of the mothers’ household as well as community members were identified as key role players in conveying information regarding breastfeeding and complementary feeding from birth to 1 year. Various factors were identified in this study that influenced suboptimal infant feeding practises in Avian Park and Zweletemba. The main factors identified were i) health, ii) physiological, iii) nutritional, iv) educational, v) behavioural, vi) financial and vii) social factors. Other aspects mentioned were viii) demographic and x) commercial factors. Conclusion Results indicated that the current practices and factors influencing the feeding practices in Avian Park and Zweletemba were similar there was very little to no cultural differences between the two communities in terms of current practices and influencing factors. The findings of this study have highlighted the importance of involving all household members in interventions, as well as the larger community in a public nutrition approach. Factors influencing current feeding practises should be considered carefully when planning future interventions to improve infant feeding practises. / AFRIKAANSE OPSOMMING: Onvanpaste voedingspraktyke is ‘n groot oorsaak van wanvoeding in jong kinders. Binne hierdie konteks is dit goed gedokumenteer dat die voorkoms van wanvoeding skerp styg gedurende die tydperk vanaf ses tot 18 maande ouderdom in die meeste lande. Komplimentêre voeding dek tipies die tydperk van ses tot 24 maande oud. Hernude fokus word geplaas op die bevordering van borsvoeding. Komplimentêre voeding behoort soortgelyke aandag te kry. Ses persent van sterftes per jaar is voorkombaar deur goeie komplimentêree voedingpraktyke. Om kindervoeding te verbeter is dit belangrik om bekend te wees met plaaslike baba- en jong kind praktyke in gemeenskappe, en ook om die onderliggende faktore wat hierdie praktyke beïnvloed te identifiseer en verstaan. Doelwit Hierdie studie het gepoog om die verskillende faktore ten opsigte van die komplimentêre voeding praktyke van babas tussen 6 – 12 maande te beskryf in 2 gemeenskappe (Avian Park en Zweletemba) in die Breede Vallei distrik van die Wes-Kaap. Metodes Die studie is uitgevoer vanaf Mei – Julie 2012. ‘n Beskrywende studie ontwerp is gebruik. ‘n Kwalitatiewe benadering is gevolg met die gebruik van fokusgroepbesprekings met moeders / primêre versorgers, vaders en oumas van babas tussen 6 – 12 maande. Resultate Die bevindinge van hierdie studie voorsien insae in die verskillende aspekte van die vroeë beëindiging van borsvoeding wat kan lei tot vroeë bekendstelling van komplimentêre voeding. In beide Avian Park en Zweletemba het die ouderdomme van insluiting van vloeistowwe en vaste stowwe gewissel van geboorte tot 12 maande.Verskeie vloeistowwe soos water, oor-die-toonbank-medisyne, hoë konsentrasie suiker drankies en lae voedingswaarde drankies was aan babas gegee so vroeg as twee dae post-partum deur middel van ‘n voedingsbottel. Koeimelk was ook gegee voor 6 maande, om redes soos bekostigbaarheid, beskikbaarheid en omdat koeimelk nie kookwater benodig vir hersamestelling soos formule melk nie, veral in situasies waar toegang tot elektrisiteit onvoldoende is. Babas van beide gemeenskappe was ook meelbol (meel en water drankie) gevoer óf via voedingsbottels (as ‘n vloeistof) of as ‘n pap wat gevoer word met ‘n lepel. Pap (veral ryspap en mieliemeelpap) was gegee vanaf een week post-partum en babas van beide gebiede het ook familie kookkos ontvang “vanuit die pot” voor 6 maande. In hierdie studie is bevind dat dit heel dikwels ‘n vrou (óf die moeder of ouma) in die huishouding is wat verantwoordelik is vir die koop en voorbereiding van voedsel asook die voer van die baba. Gesondheidswerkers, lede van die moeder se huishouding sowel as lede van die gemeenskap is geïdentifiseer as belangrike rolspelers in die oordrag van inligting oor borsvoeding en komplimentêre voeding vanaf geboorte tot een jaar. Die belangrikste faktore geïdentifiseer was verwant aan: i) gesondheid, ii) fisiologie, iii) voedingswaarde , iv) opvoedkunde, v) gedrag, vi) finansies en vii) sosiale faktore. Ander aspekte genoem is: vii) demografiese en x) kommersiële faktore. Gevolgtrekking Resultate het aangedui dat die huidige voedingpraktyke soortgelyk was in Avian Park en Zweletemba en dat daar baie min kulturele verskille tussen die twee gemeenskappe was in soverre huidige praktyke en faktore wat dit beïnvloed. Die bevindinge van hierdie studie het die belangrikheid daarvan uitgelig om al die lede van die huishouding, sowel as die breër gemeenskap in te sluit in intervensies met ‘n openbare voeding benadering. Faktore wat die huidige babavoeding praktyke beïnvloed moet versigtig oorweeg word tydens die beplanning van toekomstige intervensies om babavoeding praktyke te verbeter.

No description available.

The relationship of meal planners' nutrition attitudes and knowledge to their fat and fiber intakes and to the intakes of 2-5 year-old children in their households were examined using data from USDA's 1989-91 Continuing Survey of Food Intakes by Individuals (CSFII) and corresponding Diet and Health Knowledge Survey (DHKS). Selected households (N=478) provided 24-hour diet recalls. Data on meal planners' attitudes and knowledge were used to create variables that represented the constructs of a modified Health Belief Model. The relationships of these variables to the percent of calories from fat and to fiber density of foods consumed at home and of total food consumed by meal planners and children were analyzed using multiple regression. Several of the attitude-knowledge variables were significantly related to meal planners' fat and fiber intakes. The variables did not have a significantly different relationship with children's intakes, except for taste which was inversely related to children's fiber intakes. Although the relationship of meal planners' attitudes and knowledge was not significantly different with children's intakes than with meal planners' intakes, the constructs were not significantly related either, except for knowledge which was significantly related to less at-home fat consumption by children. Results indicate weak support for the gatekeeper theory; meal planners' nutrition knowledge and attitudes appear more operational in their diets than in the diets of their young children. / Master of Science

[Truncated abstract] Obesity is now a major public health problem worldwide. More than half the Australian population is now overweight. This is an important public health concern primarily because of the impact of overweight and obesity on risk of diabetes, hypertension and cardiovascular disease. Many strategies have been proposed to fight the obesity epidemic. One possible strategy involves understanding of the role of dietary components in the control of food intake. In this regard, dietary protein and fibre appear to be the most satiating nutrients. Foods enriched in protein, replacing energy from carbohydrate, or dietary fibre can increase satiety and reduce energy intake in the short-term. Longer-term trials suggest benefits of increasing protein or fibre intake on weight loss and features of the metabolic syndrome. The effects of dietary approaches which increase both protein and fibre at the expense of refined carbohydrate are uncertain. A practical approach to increasing both protein and fibre content of processed foods is to incorporate high protein and fibre ingredients into high carbohydrate foods. Lupin kernel flour is a novel food ingredient derived from the endosperm of lupin. It contains 40 to 45% protein, 25 to 30% fibre, and negligible sugar and starch. Lupin kernel flour can be incorporated into refined carbohydrate rich foods such as bread to increase protein and fibre content at the expense of refined carbohydrate. ... Body weight was measured every 2 weeks throughout the 16 week intervention, and these data were analysed to determine whether there was any between group difference in the rate of change in weight over 16 weeks. Over 16 weeks, lupin bread compared to white bread resulted in a significant increase in protein (13.7 (2.3, 25.0) g/d) and fibre (12.5 (8.8, 16.2) g/d) intakes, and a decrease in carbohydrate intake (-19.9 (-45.2, 5.5) g/d). There was a significant difference between groups in the rate of weight change over the 16 weeks (P=0.05). However, at 16 weeks there was no significant effect on body weight (-0.4 (-1.3, 0.6) kg), fat mass (-0.5 (-1.2, 0.2) kg) or fat free mass (0.2 (-0.5, 0.8) kg). Plasma adiponectin and leptin were not altered. Mean 24 hour systolic blood pressure (-2.4 (-3.4, -1.3) mm Hg) and pulse pressure (-3.1 (-3.9, -2.3) mm Hg) were lower for lupin relative to white bread, but diastolic blood pressure was not significantly different between groups. Apart from a lower HDL cholesterol for lupin relative to white bread (-0.09 (-0.17, -0.01) mmol/L), there were no significant differences in other blood lipids and glucose and insulin concentrations. Interpretation of the results was not influenced after adjustment for potential confounding factors. These studies assessed effects of bread enriched in lupin kernel flour relative to white bread, resulting in a higher protein and fibre intake and lower refined carbohydrate intake. This increased satiety and reduced energy intake acutely, but did not significantly influence body weight over 16 weeks. Systolic blood pressure and pulse pressure were significantly reduced. There were no significant improvements in blood lipids or glucose and insulin concentrations. Therefore, increasing protein and fibre intake at the expense of refined carbohydrate using lupin kernel flour may benefit satiety and blood pressure. Longer-term trials incorporating weight loss may be needed to observe benefits on body weight.

Thesis (Mnutr)--University of Stellenbosch, 2003. / ENGLISH ABSTRACT: Pregnancy and growth have been found to have a detrimental effect on the micronutrient status of adolescent girls. Dietary studies in adolescents have shown serious shortfalls in their dietary iron and folate intake. The competition for nutrients between the fetus and a pregnant adolescent may carry the risk of complications such as intrauterine growth retardation, pre-eclampsia, both maternal and fetal intrapartum mortality, the increased risk of birth injuries and low birth weight. The aim of the study was to assess the nutritional status of rural black, pregnant teenagers attending the antenatal clinic at Siloam Hospital in the Limpopo Province, with special emphasis on iron and folic acid intake, and evaluation of the newborn babies in terms of weight status and neural tube defects. The nutritional status was determined in 40 pregnant and 40 non-pregnant adolescent girls. The pregnant girls were selected during their first visit to the antenatal clinic, and the non-pregnant girls were selected from nearby schools. The demographic and dietary history questionnaires were used to collect information from the subjects. The dietary intake of the subjects was collected by the completion of a pre-tested quantified food frequency questionnaire. The anthropometric questionnaire was used to get information from the pregnant adolescents and the control group. The infant anthropometric measurements questionnaire provided information on the infant and the outcome of birth. Blood was collected from the pregnant adolescent girls and the control subjects. Anaemia was observed in 57.5% of the pregnant and 27.5% of the non-pregnant adolescents (haemoglobin <Il g/dl and <12 g/dl, respectively). The prevalence of low serum ferritin « 12 ug/L) and low transferrin saturation « 16%) was high in both the pregnant (30% and 60% respectively) and the non-pregnant adolescents (17% and 72.5% respectively). Iron deficiency was observed in 45% of the pregnant girls and 35% of the control subjects. The prevalence of iron deficiency anaemia in the pregnant girls (30%) and the control subjects (22.5%) was high. Low red blood cell folate in pregnant and non-pregnant girls was uncommon. Low serum vitamin BI2 was common in most of the pregnant girls. Analysis of the quantitative food frequency questionnaire (QFFQ) that was completed for 80 of the pregnant and non-pregnant girls showed that 55% had low dietary iron intakes «67% of the RDA) (p = 0.7307). Forty-five percent of the pregnant girls reported taking iron supplements. The QFFQ showed 20% of pregnant girls with low intake of folate (p = 0.0577). Forty-five percent of pregnant girls also reported taking folate supplements. Anthropometric measurements of pregnant girls and their dietary intake could not be correlated to the birth outcome. The social profile of the subjects did not seem to influence their iron and folate status and the outcome of pregnancy. The findings in this study showed that pregnant adolescents appeared to be similar to the non-pregnant adolescent girls socio-economically, anthropometric and nutritionally. We recommend that educational programmes targeted at adolescents and teenagers in the Siloam area should reach girls that could potentially fall pregnant. An educational programme must include: reproductive needs (avoidance of sex or safe sex); nutritional needs, especially targeted at improving nutritional status to meet future reproductive needs; early booking at the antenatal clinic and effective supplementation during pregnancy. The need to improve the dietary and nutrient intake of the adolescent girls should be addressed within the current framework of the Integrated Nutrition Programme (!NP). A food fortification programme with essential micronutrients such as iron, folic acid, zinc and Vitamin A to improve the micronutrient status should be promoted among the adolescent girls. / AFRIKAANSE OPSOMMING: Daar is bevind dat swangerskap en groei 'n nadelige effek het op die mikronutriëntstatus van vroulike adolessente. Dieetstudies in adolessente het ernstige tekortkominge in dieetyster- en folaatinnames getoon. Die kompetisie vir nutriente tussen die fetus en die swanger adolessent kan verantwoordelik wees vir komplikasies soos intra-uterine groeivertraging, preeklampsie, verhoogde mortaliteit van beide moeder en baba tydens kraam, 'n verhoogde risiko vir geboortebeserings en lae geboortegewig. Die doel van die studie was om die effek van voedingstatus by swart, swanger tieners by die voorgeboortekliniek in Siloam Hospital in die Limpopo-provinsie te bepaal, met spesifieke verwysing na die yster- en foliensuurinname, asook die evaluering van die pasgebore babas in terme van gewig en neurale buis defekte. Die voedingstatus van 40 swanger en 40 nie-swanger adolessente meisies IS bepaal. Die swanger meisies is ewekansig geselekteer gedurende die eerste besoek aan die voorgeboortekliniek , en die nie-swanger meisies is geselekteer by nabygeleë skole. Die demografiese en dieetgeskiedenisvraelyste is gebruik om inligting van die proefpersone in te samel. Voorafgetoetste gekwantifiseerde voedselfrekwensie vraelyste is gebruik om die voedselinname van proefpersone te bepaal. Antropometriese vraelyste is gebruik om antropometriese inligting van die swanger adolessente en die kontrole groep. Die antropometriese vraelys vir babas is gebruik om inligting ten opsigte van die baba aan te teken asook die verloop van die swangerskap. Bloedmonsters is van die swanger tieners en die kontrole groep ingesamel. Anemie is waargeneem by 57.5% van die swanger en 27.5% van die nie-swanger adolessente (hemoglobien <Il g/dl en <12 g/dl onderskeidelik). Die voorkoms van lae serum ferritien « 12 ugIL) en lae transferrienversadiging « 16%) was hoog by beide die swanger (30% en 60%) en die nie-swanger adolessente (17.5% en 72.5% onderskeidelik). Ystergebrek is in 45% van die swanger meisies en in 35% van die kontrole groep waargeneem. Die voorkoms van ystergebrekanemie in die swanger meisies (30%) en die kontrole groep (22.5%) was hoog. Lae rooibloedselfolaat by swanger en nie-swanger meisies is nie algemeen waargeneem nie. Lae serum vitamien B12 was algemeen by die meeste swanger meisies. Ontleding van die gekwantifiseerde voedselfrekwensievraelys (KVFV) wat vir 80 van die swanger en nie-swanger meisies voltooi is, het getoon dat 55% 'n lae dieetysterinname gehad het «67% van die ADT) (p=0.7307). Vyf-en- veertig persent van die swanger meisies het bevestig dat hulle ystersupplemente gebruik het. Die gekwantifiseerde voedselfrekwensievraelys (KVFV) het getoon dat 20% van die swanger meisies 'n lae folaatinname het (0.0577). Vyf-en-veertig persent van die swanger meisies het ook genoem dat hulle folaatsupplemente gebruik het. Die antropometriese metings van swanger meisies en dieetinname kon nie gekorreleer word met die verloop van die geboorte nie. Dit blyk dat die sosiale profiel van die meisies nie 'n effek op die yster- en folaatstatus en op die verloop van swangerskap gehad het nie. Die bevindinge van hierdie studie toon dat swanger en nie-swanger adolessente meisies sosio-ekonomies, antropometries en nutrisioneel dieselfde voorkom. Dit word aanbeveel dat voorligtingsprogramme vir adolessente en tieners in die Siloam-area meisies moet bereik wat moontlik swanger sal word. 'n Voorligtingprogram moet die volgende insluit: reproduktiewe behoeftes (vermyding van seks of veilige seks); voedingbehoeftes, veral geteiken om voedingstatus te verbeter om ten einde toekomstige voorplantingsbehoeftes te vervul; vroeë besoeke aan die voorgeboortekliniek en effektiewe supplementering gedurende swangerskap. Die behoefte om die dieet en nutriëntinname van adolessente meisies te verbeter moet binne die huidige raamwerk van die Geintegreerde Voedingsprogram aangespreek word. 'n Voedselfortfiseringsprogram met essensiële mikronutriënte soos yster, foliensuur, sink en vitamien A om die mikronutriëntstatus van adolessente meisies te verbeter, moet bevorder word.

Mineral binding capacity of rice bran, oat hull, soy bran, wheat bran, peanut, apple, tomato, and barley fiber were investigated. Defatted fibers were analyzed for ash, protein, acid detergent fiber, and lignin. The fibers were washed with 1% HCl and Acid washed samples were studied for their mineral binding ability. The minerals tested were zinc, copper, and magnesium. Analysis of acid washed samples showed that most of the original minerals had been stripped from the fiber. In most of the fibers studied, the order of binding was copper > zinc > magnesium. Among the eight fibers studied, oat hull and apple fiber had the lowest consistent binding capacity for the three minerals investigated. This in vitro binding study has been run to duplicate intestinal condition of pH 6.8.

The objective of this study was to compare the effects on eating behavior, lipids, lipoproteins, lipid peroxidation, and glycemic control in women with type 2 diabetes of a high-monounsaturated fat diet (HM) compared to a high-carbohydrate diet (HC). In an outpatient feeding study, ten hypertriglyceridemic postmenopausal type 2 diabetic women alternately for six weeks consumed the HM and HC diets. On the HM diet, 45% of total calories were consumed as carbohydrate and 40% as fat (27% monounsaturated) compared to 55% carbohydrate and 30% fat (10% monounsaturated) in the HC diet. At the beginning and end of each diet phase, total lipids, lipoproteins, lipid peroxidation, and glycemic variables were measured. For 8 days in each diet phase eating pattern frequency, palatability of foods, hunger and fullness were assessed. At the end of each diet phase, taste testing to determine preference for fat was conducted. Total cholesterol was significantly decreased on the HC diet. Serum triglyceride, very low density lipoprotein (VLDL) triglyceride and cholesterol, and apolipoproteins A-1 and B were not significantly different on the two diets. When comparing initial to final values, both diets lowered LDL-C; however, the change was greater on the HM diet. Lipid peroxidation variables improved when the HM diet was consumed. Glycemic variables improved on both diets. No significant differences between total number of eating episodes on the HM and HC diet phases were found. Both diets were rated as highly palatable. Hunger and fullness ratings varied within and between subjects. However, fullness was more commonly experienced than hunger on both HM and the HC diet. Preference for fat was not found at the end ofHM or HC diets. However, subjects differed significantly in ratings for liking of foods that were salty, sour, and bitter when compared to nondiabetic women. Consumption of the HM and HC diets did not result in deterioration of lipid status. The HM diet by virtue of less oxidation of the LDL particle and improvement of glycemic control provides an important advantage over the HC diet. A description of eating behavior of women with type 2 diabetes emerged. / Graduation date: 2000

The effect of vitamin B-6 (B-6) status on plasma fatty acids (FA) levels and lipid metabolism was investigated in this metabolic study. Eight female subjects were fed for 28 days. For the first 7 days, they were fed a constant diet containing 2.10 mg of B-6. For the rest of the period (21 days), they were differentiated in terms of B-6 intake; 4 of them were fed a low (0.93 mg/day) and 4 a high (2.60 mg/day) B-6 diet. B-6 status indices, plasma FA concentration and lipid profile were determined. Plasma pyridoxal 5'-phosphate and total B-6 concentration (P<0.01), urinary 4- pyridoxic acid and total B-6 concentration (P<0.001) showed a significant difference between the two groups at the end of the study. Erythrocyte PLP failed to show any significant difference between the two groups throughout the diet study. There was no significant difference in the plasma FA or lipid profile between the two groups. Plasma total cholesterol (TC) of the low B-6 group decreased slightly (7 %), but was not statistically significant. When comparing day 7 and day 28 values, plasma triglycerides increased (9 %) for the high and decreased for the low B-6 group. LDL-C decreased (5 %) for the high B-6 group but did not change in the low B-6 group. HDL-C decreased slightly in both groups (~8 %). There was no clear evidence that a low intake of vitamin B-6 affects the fatty acid and lipid metabolism. Further studies are required to identify the relationship between vitamin B-6 and fatty acid and lipid metabolism in humans. / Graduation date: 1997