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The relationship of meal planners' nutrition attitudes and knowledge to their fat and fiber intakes and to the intakes of 2-5 year-old children in their households were examined using data from USDA's 1989-91 Continuing Survey of Food Intakes by Individuals (CSFII) and corresponding Diet and Health Knowledge Survey (DHKS). Selected households (N=478) provided 24-hour diet recalls. Data on meal planners' attitudes and knowledge were used to create variables that represented the constructs of a modified Health Belief Model. The relationships of these variables to the percent of calories from fat and to fiber density of foods consumed at home and of total food consumed by meal planners and children were analyzed using multiple regression. Several of the attitude-knowledge variables were significantly related to meal planners' fat and fiber intakes. The variables did not have a significantly different relationship with children's intakes, except for taste which was inversely related to children's fiber intakes. Although the relationship of meal planners' attitudes and knowledge was not significantly different with children's intakes than with meal planners' intakes, the constructs were not significantly related either, except for knowledge which was significantly related to less at-home fat consumption by children. Results indicate weak support for the gatekeeper theory; meal planners' nutrition knowledge and attitudes appear more operational in their diets than in the diets of their young children. / Master of Science

[Truncated abstract] Obesity is now a major public health problem worldwide. More than half the Australian population is now overweight. This is an important public health concern primarily because of the impact of overweight and obesity on risk of diabetes, hypertension and cardiovascular disease. Many strategies have been proposed to fight the obesity epidemic. One possible strategy involves understanding of the role of dietary components in the control of food intake. In this regard, dietary protein and fibre appear to be the most satiating nutrients. Foods enriched in protein, replacing energy from carbohydrate, or dietary fibre can increase satiety and reduce energy intake in the short-term. Longer-term trials suggest benefits of increasing protein or fibre intake on weight loss and features of the metabolic syndrome. The effects of dietary approaches which increase both protein and fibre at the expense of refined carbohydrate are uncertain. A practical approach to increasing both protein and fibre content of processed foods is to incorporate high protein and fibre ingredients into high carbohydrate foods. Lupin kernel flour is a novel food ingredient derived from the endosperm of lupin. It contains 40 to 45% protein, 25 to 30% fibre, and negligible sugar and starch. Lupin kernel flour can be incorporated into refined carbohydrate rich foods such as bread to increase protein and fibre content at the expense of refined carbohydrate. ... Body weight was measured every 2 weeks throughout the 16 week intervention, and these data were analysed to determine whether there was any between group difference in the rate of change in weight over 16 weeks. Over 16 weeks, lupin bread compared to white bread resulted in a significant increase in protein (13.7 (2.3, 25.0) g/d) and fibre (12.5 (8.8, 16.2) g/d) intakes, and a decrease in carbohydrate intake (-19.9 (-45.2, 5.5) g/d). There was a significant difference between groups in the rate of weight change over the 16 weeks (P=0.05). However, at 16 weeks there was no significant effect on body weight (-0.4 (-1.3, 0.6) kg), fat mass (-0.5 (-1.2, 0.2) kg) or fat free mass (0.2 (-0.5, 0.8) kg). Plasma adiponectin and leptin were not altered. Mean 24 hour systolic blood pressure (-2.4 (-3.4, -1.3) mm Hg) and pulse pressure (-3.1 (-3.9, -2.3) mm Hg) were lower for lupin relative to white bread, but diastolic blood pressure was not significantly different between groups. Apart from a lower HDL cholesterol for lupin relative to white bread (-0.09 (-0.17, -0.01) mmol/L), there were no significant differences in other blood lipids and glucose and insulin concentrations. Interpretation of the results was not influenced after adjustment for potential confounding factors. These studies assessed effects of bread enriched in lupin kernel flour relative to white bread, resulting in a higher protein and fibre intake and lower refined carbohydrate intake. This increased satiety and reduced energy intake acutely, but did not significantly influence body weight over 16 weeks. Systolic blood pressure and pulse pressure were significantly reduced. There were no significant improvements in blood lipids or glucose and insulin concentrations. Therefore, increasing protein and fibre intake at the expense of refined carbohydrate using lupin kernel flour may benefit satiety and blood pressure. Longer-term trials incorporating weight loss may be needed to observe benefits on body weight.

Thesis (Mnutr)--University of Stellenbosch, 2003. / ENGLISH ABSTRACT: Pregnancy and growth have been found to have a detrimental effect on the micronutrient status of adolescent girls. Dietary studies in adolescents have shown serious shortfalls in their dietary iron and folate intake. The competition for nutrients between the fetus and a pregnant adolescent may carry the risk of complications such as intrauterine growth retardation, pre-eclampsia, both maternal and fetal intrapartum mortality, the increased risk of birth injuries and low birth weight. The aim of the study was to assess the nutritional status of rural black, pregnant teenagers attending the antenatal clinic at Siloam Hospital in the Limpopo Province, with special emphasis on iron and folic acid intake, and evaluation of the newborn babies in terms of weight status and neural tube defects. The nutritional status was determined in 40 pregnant and 40 non-pregnant adolescent girls. The pregnant girls were selected during their first visit to the antenatal clinic, and the non-pregnant girls were selected from nearby schools. The demographic and dietary history questionnaires were used to collect information from the subjects. The dietary intake of the subjects was collected by the completion of a pre-tested quantified food frequency questionnaire. The anthropometric questionnaire was used to get information from the pregnant adolescents and the control group. The infant anthropometric measurements questionnaire provided information on the infant and the outcome of birth. Blood was collected from the pregnant adolescent girls and the control subjects. Anaemia was observed in 57.5% of the pregnant and 27.5% of the non-pregnant adolescents (haemoglobin <Il g/dl and <12 g/dl, respectively). The prevalence of low serum ferritin « 12 ug/L) and low transferrin saturation « 16%) was high in both the pregnant (30% and 60% respectively) and the non-pregnant adolescents (17% and 72.5% respectively). Iron deficiency was observed in 45% of the pregnant girls and 35% of the control subjects. The prevalence of iron deficiency anaemia in the pregnant girls (30%) and the control subjects (22.5%) was high. Low red blood cell folate in pregnant and non-pregnant girls was uncommon. Low serum vitamin BI2 was common in most of the pregnant girls. Analysis of the quantitative food frequency questionnaire (QFFQ) that was completed for 80 of the pregnant and non-pregnant girls showed that 55% had low dietary iron intakes «67% of the RDA) (p = 0.7307). Forty-five percent of the pregnant girls reported taking iron supplements. The QFFQ showed 20% of pregnant girls with low intake of folate (p = 0.0577). Forty-five percent of pregnant girls also reported taking folate supplements. Anthropometric measurements of pregnant girls and their dietary intake could not be correlated to the birth outcome. The social profile of the subjects did not seem to influence their iron and folate status and the outcome of pregnancy. The findings in this study showed that pregnant adolescents appeared to be similar to the non-pregnant adolescent girls socio-economically, anthropometric and nutritionally. We recommend that educational programmes targeted at adolescents and teenagers in the Siloam area should reach girls that could potentially fall pregnant. An educational programme must include: reproductive needs (avoidance of sex or safe sex); nutritional needs, especially targeted at improving nutritional status to meet future reproductive needs; early booking at the antenatal clinic and effective supplementation during pregnancy. The need to improve the dietary and nutrient intake of the adolescent girls should be addressed within the current framework of the Integrated Nutrition Programme (!NP). A food fortification programme with essential micronutrients such as iron, folic acid, zinc and Vitamin A to improve the micronutrient status should be promoted among the adolescent girls. / AFRIKAANSE OPSOMMING: Daar is bevind dat swangerskap en groei 'n nadelige effek het op die mikronutriëntstatus van vroulike adolessente. Dieetstudies in adolessente het ernstige tekortkominge in dieetyster- en folaatinnames getoon. Die kompetisie vir nutriente tussen die fetus en die swanger adolessent kan verantwoordelik wees vir komplikasies soos intra-uterine groeivertraging, preeklampsie, verhoogde mortaliteit van beide moeder en baba tydens kraam, 'n verhoogde risiko vir geboortebeserings en lae geboortegewig. Die doel van die studie was om die effek van voedingstatus by swart, swanger tieners by die voorgeboortekliniek in Siloam Hospital in die Limpopo-provinsie te bepaal, met spesifieke verwysing na die yster- en foliensuurinname, asook die evaluering van die pasgebore babas in terme van gewig en neurale buis defekte. Die voedingstatus van 40 swanger en 40 nie-swanger adolessente meisies IS bepaal. Die swanger meisies is ewekansig geselekteer gedurende die eerste besoek aan die voorgeboortekliniek , en die nie-swanger meisies is geselekteer by nabygeleë skole. Die demografiese en dieetgeskiedenisvraelyste is gebruik om inligting van die proefpersone in te samel. Voorafgetoetste gekwantifiseerde voedselfrekwensie vraelyste is gebruik om die voedselinname van proefpersone te bepaal. Antropometriese vraelyste is gebruik om antropometriese inligting van die swanger adolessente en die kontrole groep. Die antropometriese vraelys vir babas is gebruik om inligting ten opsigte van die baba aan te teken asook die verloop van die swangerskap. Bloedmonsters is van die swanger tieners en die kontrole groep ingesamel. Anemie is waargeneem by 57.5% van die swanger en 27.5% van die nie-swanger adolessente (hemoglobien <Il g/dl en <12 g/dl onderskeidelik). Die voorkoms van lae serum ferritien « 12 ugIL) en lae transferrienversadiging « 16%) was hoog by beide die swanger (30% en 60%) en die nie-swanger adolessente (17.5% en 72.5% onderskeidelik). Ystergebrek is in 45% van die swanger meisies en in 35% van die kontrole groep waargeneem. Die voorkoms van ystergebrekanemie in die swanger meisies (30%) en die kontrole groep (22.5%) was hoog. Lae rooibloedselfolaat by swanger en nie-swanger meisies is nie algemeen waargeneem nie. Lae serum vitamien B12 was algemeen by die meeste swanger meisies. Ontleding van die gekwantifiseerde voedselfrekwensievraelys (KVFV) wat vir 80 van die swanger en nie-swanger meisies voltooi is, het getoon dat 55% 'n lae dieetysterinname gehad het «67% van die ADT) (p=0.7307). Vyf-en- veertig persent van die swanger meisies het bevestig dat hulle ystersupplemente gebruik het. Die gekwantifiseerde voedselfrekwensievraelys (KVFV) het getoon dat 20% van die swanger meisies 'n lae folaatinname het (0.0577). Vyf-en-veertig persent van die swanger meisies het ook genoem dat hulle folaatsupplemente gebruik het. Die antropometriese metings van swanger meisies en dieetinname kon nie gekorreleer word met die verloop van die geboorte nie. Dit blyk dat die sosiale profiel van die meisies nie 'n effek op die yster- en folaatstatus en op die verloop van swangerskap gehad het nie. Die bevindinge van hierdie studie toon dat swanger en nie-swanger adolessente meisies sosio-ekonomies, antropometries en nutrisioneel dieselfde voorkom. Dit word aanbeveel dat voorligtingsprogramme vir adolessente en tieners in die Siloam-area meisies moet bereik wat moontlik swanger sal word. 'n Voorligtingprogram moet die volgende insluit: reproduktiewe behoeftes (vermyding van seks of veilige seks); voedingbehoeftes, veral geteiken om voedingstatus te verbeter om ten einde toekomstige voorplantingsbehoeftes te vervul; vroeë besoeke aan die voorgeboortekliniek en effektiewe supplementering gedurende swangerskap. Die behoefte om die dieet en nutriëntinname van adolessente meisies te verbeter moet binne die huidige raamwerk van die Geintegreerde Voedingsprogram aangespreek word. 'n Voedselfortfiseringsprogram met essensiële mikronutriënte soos yster, foliensuur, sink en vitamien A om die mikronutriëntstatus van adolessente meisies te verbeter, moet bevorder word.

Mineral binding capacity of rice bran, oat hull, soy bran, wheat bran, peanut, apple, tomato, and barley fiber were investigated. Defatted fibers were analyzed for ash, protein, acid detergent fiber, and lignin. The fibers were washed with 1% HCl and Acid washed samples were studied for their mineral binding ability. The minerals tested were zinc, copper, and magnesium. Analysis of acid washed samples showed that most of the original minerals had been stripped from the fiber. In most of the fibers studied, the order of binding was copper > zinc > magnesium. Among the eight fibers studied, oat hull and apple fiber had the lowest consistent binding capacity for the three minerals investigated. This in vitro binding study has been run to duplicate intestinal condition of pH 6.8.

The objective of this study was to compare the effects on eating behavior, lipids, lipoproteins, lipid peroxidation, and glycemic control in women with type 2 diabetes of a high-monounsaturated fat diet (HM) compared to a high-carbohydrate diet (HC). In an outpatient feeding study, ten hypertriglyceridemic postmenopausal type 2 diabetic women alternately for six weeks consumed the HM and HC diets. On the HM diet, 45% of total calories were consumed as carbohydrate and 40% as fat (27% monounsaturated) compared to 55% carbohydrate and 30% fat (10% monounsaturated) in the HC diet. At the beginning and end of each diet phase, total lipids, lipoproteins, lipid peroxidation, and glycemic variables were measured. For 8 days in each diet phase eating pattern frequency, palatability of foods, hunger and fullness were assessed. At the end of each diet phase, taste testing to determine preference for fat was conducted. Total cholesterol was significantly decreased on the HC diet. Serum triglyceride, very low density lipoprotein (VLDL) triglyceride and cholesterol, and apolipoproteins A-1 and B were not significantly different on the two diets. When comparing initial to final values, both diets lowered LDL-C; however, the change was greater on the HM diet. Lipid peroxidation variables improved when the HM diet was consumed. Glycemic variables improved on both diets. No significant differences between total number of eating episodes on the HM and HC diet phases were found. Both diets were rated as highly palatable. Hunger and fullness ratings varied within and between subjects. However, fullness was more commonly experienced than hunger on both HM and the HC diet. Preference for fat was not found at the end ofHM or HC diets. However, subjects differed significantly in ratings for liking of foods that were salty, sour, and bitter when compared to nondiabetic women. Consumption of the HM and HC diets did not result in deterioration of lipid status. The HM diet by virtue of less oxidation of the LDL particle and improvement of glycemic control provides an important advantage over the HC diet. A description of eating behavior of women with type 2 diabetes emerged. / Graduation date: 2000

The effect of vitamin B-6 (B-6) status on plasma fatty acids (FA) levels and lipid metabolism was investigated in this metabolic study. Eight female subjects were fed for 28 days. For the first 7 days, they were fed a constant diet containing 2.10 mg of B-6. For the rest of the period (21 days), they were differentiated in terms of B-6 intake; 4 of them were fed a low (0.93 mg/day) and 4 a high (2.60 mg/day) B-6 diet. B-6 status indices, plasma FA concentration and lipid profile were determined. Plasma pyridoxal 5'-phosphate and total B-6 concentration (P<0.01), urinary 4- pyridoxic acid and total B-6 concentration (P<0.001) showed a significant difference between the two groups at the end of the study. Erythrocyte PLP failed to show any significant difference between the two groups throughout the diet study. There was no significant difference in the plasma FA or lipid profile between the two groups. Plasma total cholesterol (TC) of the low B-6 group decreased slightly (7 %), but was not statistically significant. When comparing day 7 and day 28 values, plasma triglycerides increased (9 %) for the high and decreased for the low B-6 group. LDL-C decreased (5 %) for the high B-6 group but did not change in the low B-6 group. HDL-C decreased slightly in both groups (~8 %). There was no clear evidence that a low intake of vitamin B-6 affects the fatty acid and lipid metabolism. Further studies are required to identify the relationship between vitamin B-6 and fatty acid and lipid metabolism in humans. / Graduation date: 1997

While replacement of dietary saturated fat with unsaturated fat has been advocated to reduce cardiovascular disease risk, diets high in polyunsaturated fatty acids (PUFA) could increase low density lipoprotein (LDL) susceptibility to oxidation, potentially contributing to the pathology of atherosclerosis. To assess in vivo lipid peroxidation and susceptibility, of LDL surface and core lipids to ex vivo oxidation, in women consuming increased amounts of specific unsaturated fatty acids, 15 postmenopausal women took daily supplements of sunflower oil providing 12.3 g/day of oleate, safflower oil providing 10.5 g/day of linoleate, and fish oil providing 2.0 g/day of eicosapentaenoate (EPA) and 1.4 g/day of docosahexaenoate (DHA) during a crossover trial. Plasma F₂-isoprostanes (F₂-isoP), malondialdehyde (MDA), and thiobarbituric acid reacting substances (TEARS) were measured to assess lipid peroxidation in vivo. Ex vivo oxidation of LDL was monitored by measuring the formation of phosphatidylcholine hydroperoxides (PCOOH) and cholesteryl linoleate hydroperoxides (CE18:200H) during coppermediated oxidation. Plasma free F₂-isoP and MDA concentrations were lower after EPA/DHA supplementation than after oleate (P = 0.001, F₂-isoP and 0.02, MDA) and linoleate supplementation (P = 0.04 for both F₂-isoP and MDA). However, plasma TBARS concentrations were higher after EPA/DHA than after oleate (P = 0.001) and linoleate supplementation (P = 0.0004). During LDL oxidation, the lag phase for PCOOH formation was shorter in EPA/DHA- than oleate- (P = 0.0001) and linoleate-enriched LDL (P = 0.002), while the lag phase for CE18:200H was shorter in EPA/DHA- than oleate- (P = 0.01) but not linoleate-enriched LDL. The maximal rate of PCOOH formation was lower in EPA/ DHA- than linoleate- (P = 0.007) but not oleate-enriched LDL, while the maximal rate of CE18:200H formation was lower in EPA/DHA- than oleate- (P = 0.03) and linoleate-enriched LDL (P [less than or equal to] 0.0001). The maximal concentrations of PCOOH and CE18:200H were lower in EPA/DHA- than oleate- (P [less than or equal to] 0.05) and linoleate-enriched LDL (P [less than or equal to] 0.01). Oleate-enrichment generally decreased the oxidative susceptibility of LDL surface and core lipids, while EPA/DHA-enrichment did not increase LDL oxidative susceptibility compared to linoleate-enrichment. This study emphasizes the need for more than one relevant assay of in vivo lipid peroxidation. / Graduation date: 2000

The purpose of this study was to examine the effect of extreme exercise on vitamin B-6 metabolism and urea nitrogen. Nine men and five women completed two 5-day trials; Trial 1 (T1) included a 50-km ultramarathon on day 4 and during Trial 2 (T2) subjects were "inactive" on day 4. During both trials, subjects consumed a diet providing men 2.0 and women 1.5 mg of vitamin B-6. With the exception of the ultramarathon, T1 activity was replicated during T2. Twenty four-hour urine collections were completed and blood was drawn pre-race (pre), mid-race (mid), post-race (post) and 60 minutes post race (P-60). On the inactive, day blood was drawn at the same intervals. Plasma was analyzed for pyridoxal 5'-phosphate (PLP), pyridoxal, 4-pyridoxic acid (4-PA), urea nitrogen (PUN), creatinine, albumin, glucose, and lactate concentration and alkaline phosphatase activity. Urine was analyzed for 4PA, creatinine, and total urinary nitrogen (TUN). During T1, compared to pre, plasma PLP concentration increased 17% at mid, decreased 5% by post, and 19% by P-60. During T2, plasma PLP concentration decreased 13% pre to P-60. During T1, plasma 4-PA concentration increased 135% and the percent dietary vitamin B-6 that was excreted as urinary 4-PA the day of the ultramarathon was higher than that excreted the day before and the day after. During T1, from pre to post mean PUN concentration increased 36.9%, and the average rate ofincrease from pre to mid, mid to post, and post to P60 was 0.5, 1.75, and 2 mg/dL/hour, respectively. During T1 on days 3, 4, and 5,88%, 100%, and 95% of nitrogen intake was excreted in the urine compared to 86%, 83%, and 84% for the same days during T2. The day of the ultramarathon, 24-hour TUN excretion was 2 g higher than the previous day. Extreme exercise of greater than six hours initially increases the plasma concentration of PLP but ultimately results in a significant decrease in plasma PLP, an increase in plasma 4-PA, and an increase in percent of dietary vitamin B-6 (as 4-PA) excreted in the urine. Additionally, the rate of change in PUN inoeases as duration increases. / Graduation date: 2001

This investigation was conducted to determine the effect of estrogen replacement therapy (ERT) on vitamin B-6 status of postmenopausal women. Nineteen postmenopausal women served as subjects. Nine (54.7 + 4.7 years) were taking ERT (experimental group); ten (56.8 + 2.3 years) were not (control group). For three consecutive days, subjects recorded their dietary intake and collected their 24-hour urine specimens. On the fourth day, a fasting blood sample was drawn from the subjects. The dietary intake of vitamin B-6, as well as the concentration of total vitamin B-6 in plasma (PB6; and urine (UB6) were measured. PB6 and UB6 were determined by a microbiological method with Saccharomyces uvarum as the assay organism. The mean age, height, hematocrit and hemoglobin values were similar for the two groups. The experimental group was significantly heavier than the control group (p<0.05). The experimental group had a lower mean PB6 than the control group: 47.7 ± 19.7 nmol/L vs. 56.2 + 20.6 nmol/L. These means were not significantly different (p=0.05). PB6 was positively correlated with dietary vitamin B-6 intake (p=0.0001) and vitamin B-6 to protein ratio (p=0.0021). When the means were adjusted for dietary vitamin B-6 and the vitamin B-6 to protein ratio, the mean PB6 of the experimental group (42.7 nmol/L) was significantly lower than that of the control group (60.6 nmol/L) (p<0.05). PB6 was not positively correlated with either age (r=0.20) or the vitamin B-6 dietary history score (r=0.15). UB6 was similar for the two groups. UB6 correlated positively with daily dietary intake of vitamin B-6 (r=0.51, p<0.05) and the ratio of vitamin B-6 to protein (r=0.47, p<0.05), UB6 was not significantly correlated to urine volume (r=0.05). The mean daily intakes of vitamin B-6 and protein were similar for the two groups. One of the 19 subjects had a vitamin B-6 intake that was less than 67 percent of the RDA. Most subjects' (89%) intake of vitamin B-6 was adequate when the ratio of 0.016 mg of vitamin B-6 per g of protein was used as the standard. / Graduation date: 1990