A nitrogen fertility study with Vaughn’s hybrid bermudagrass conducted on a Crider silt loam soil (fine, silty, mixed, active, mesic Typic Paleudalfs) over three (3) years (2008-2011) at the Highland Rim Research and Education Center near Springfield, Tennessee is evaluated in this manuscript. Nitrogen applications are evaluated in both irrigated and non-irrigated plots at five (5) different application rates: 0, 56, 112, 168, and 224 kg N ha-1. These rates are applied beginning in late April, and three (3) additional times upon harvests occurring in June, July, and August. Irrigation plots receive enough water to bring total weekly water up to 2.24 cm/plot whenever rainfall is less than that amount. Normalized difference vegetative index (NDVI) measurements are collected mid harvest and on harvest dates to investigate new nitrogen status indicators between Vaughn’s hybrid bermudagrass yields. Plant tissue samples are collected at harvest. Soil samples are collected mid harvest to investigate soil nitrate nitrogen and its relationship with bermudagrass yields. The results of the study show irrigation has no effect on yields during the period of this study. There is a significant effect resulting from the interaction between month and nitrogen application on yield. Investigation of this interaction reveals two (2) distinct periods of production potential during the growing season. A low to medium yielding period produces an average harvest yield maximum of 3.14 Mg ha-1. A medium to high yield period produces an average harvest yield maximum of 5.4 Mg ha-1. Based on an analysis of variance and mean separation, a nitrogen rate of 56 kg N ha-1 rate is recommended for harvests occurring during the low to medium yielding period, and a nitrogen rate of 113 kg N ha-1 is recommended for those occurring during the high to medium yielding period. NDVI is highly correlated with yield on date of harvest. The results also show NDVI is correlated with mid-harvest yields also, which suggests a possible development of using NDVI as a mid harvest nitrogen status indicator. The results show soil nitrate is not correlated with yield, but did indicate accumulation in the soil as the growing season progressed.

Two types of novel framework materials, including six organically templated silicogermanates and five hybrid fluorotitanates, have been synthesized, and their structures have been determined by single crystal X-ray diffraction. Their structures and the interactions between the organic and inorganic species are discussed in some detail. The frameworks of the six silicogermanates, SU-9, SU-10, SU-11, SU-12, SU-14 and SU-21, are built up from either tetrahedra exclusively, or from mixed polyhedra. SU-9, SU-10 and SU-11 consist only of tetrahedra, and SU-12, SU-14 and SU-21 are built up from mixed polyhedra. In all silicogermanates, Si and Ge share the tetrahedrally coordinated positions, and only Ge occupies the 5- and 6-coordinated positions. The frameworks of SU-9, SU-10 and SU-11 belong to zeolite frameworks. SU-9 and SU-10 are built up from the same periodic building units (PBUs) that are formed by 4/4-4 units, and they adopt the known zeolite framework types AST and ASV, respectively. SU-11 is built up from 5-1 units and adopts the known zeolite framework type MFI. This is the first time that the frameworks of AST, ASV and MFI have been prepared in silicogermanate form. The 3-D frameworks of SU-12 and SU-14 contain (Ge,Si)7 composite building units that form one-dimensional 24-ring channels along the c axis. Their structures contain intersecting channels. SU-12 contains 8-, 10- 12- and 24-ring channels; while SU-14 has 9-, 10-, 12- and 24-ring channels. SU-21 is a two-dimensional framework structure containing 10-ring channels. It is the first silicogermanate with the template covalently bonded to Ge. All silicogermanates were synthesized by using organic amines as templates. The templates play a very important role in directing the structure of the inorganic framework. The obtained five hybrid fluorotitanates are SUF-1, SUF-2, SUF-3, SUF-4 and SUF-5. Their hybrid frameworks are built up from fluorotitanate anions, N-containing cations and crystal water/oxonium cations. Zero-dimensional fluorotitanate clusters are normally obtained when cyclic organic amines such as 1,4-diazabicyclo[2,2,2]octane (DABCO) and piperazine are used. Increasing the pH value of the starting solutions led to more condensed fluorotitanate anions, from isolated octahedra to pairs and eventually chains of octahedra. The formation of the hybrid frameworks of fluorotitanates is strongly dependent on hydrogen bonds between the fluorotitanate anions, N-containing organic cations and water molecules/oxonium ions.

This thesis (1) reports the development of simulation tools and control strategies for optimizing hybrid electric vehicle (HEV) energy management, and (2) reports the design and testing of a hydraulic hybrid school bus (HHB) prototype. A hybrid vehicle is one that combines two or more energy sources for use in vehicle propulsion. Hybrid electric vehicles have become popular in the consumer market due to their greatly improved fuel economy over conventional vehicles. The control strategy of an HEV has a paramount effect on its fuel economy performance. In this thesis, backward-looking and forward-looking simulations of three HEV architectures (parallel, power-split and 2-mode power-split) are developed. The Equivalent Cost Minimization Strategy (ECMS), which weights electrical power as an equivalent fuel usage, is then studied in great detail and improvements are suggested. Specifically, the robustness of an ECMS controller is improved by linking the equivalence factor to dynamic programming and then further tailoring its functional form. High-fidelity vehicle simulations over multiple drive-cycles are performed to measure the improved performance of the new ECMS controller, and to show its potential for online application. While HEVs are prominent in the consumer market and studied extensively in current literature, hydraulic hybrid vehicles (HHVs) only exist as heavy utility vehicle prototypes. The second half of this thesis reports design, construction, and testing of a hydraulic hybrid school bus prototype. Design considerations, simulation results, and preliminary testing results are reported, which indicate the strong potential for hydraulic hybrids to improve fuel economy in the school bus vehicle segment.

DNA post-replication repair (PRR) is a cellular tolerance mechanism by which eukaryotic cells survive lethal lesions during or after DNA synthesis. In the yeast Saccharomyces cerevisiae, modification of proliferating cell nuclear antigen (PCNA) by ubiquitin and by small ubiquitin-like modifier (SUMO) plays an important role in PRR. PCNA ubiquitination is dependent on Rad6, a ubiquitin-conjugating enzyme (E2) and Rad18, a ubiquitin ligase (E3). Rad6 and Rad18 form a stable complex. PCNA sumoylation is dependent on Ubc9, an E2 specific to SUMO modification. <p>PRR in mammalian cells is less well understood. However, human Rad18 (hRad18) has been found to interact with human Rad6 (HHR6A/B). In this study, we detected physical interaction between hRad18 and human Ubc9 (hUbc9) through yeast two-hybrid assays. In order to define the domain(s) of hRad18 involved in the formation of a complex with HHR6B or hUbc9, a series of yeast two-hybrid constructs containing various hRAD18 gene deletions and mutations were made. A C-terminal region of hRad18, containing the putative HHR6A/B binding domain (amino acids 340 to 395), interacts with HHR6A/B while the N-terminus (amino acids 1-93) does not. Yeast Rad18 has a homologous fragment of the HHR6A/B binding domain and this fragment is sufficient to interact with yeast Rad6 in yeast two-hybrid assays, so we infer that hRad18 interacts with HHR6B through the same domain. Surprisingly, both the N-terminal and C-terminal fragments of hRad18 can interact with hUbc9, suggesting the existence of two separate domains in hRad18 interacting with hUbc9. The N-terminal fragment of hRad18 contains only a RING finger domain (amino acids 25-64), which is probably responsible for binding to hUbc9. The C-terminal fragment of hRad18 with HHR6A/B binding domain deletion can still interact with hUbc9, suggesting that the HHR6A/B binding domain is not involved in hUbc9 interaction. A key cysteine mutation (C28F) in the RING finger domain abolished the interactions of hRad18 with both HHR6A/B and hUbc9. This amino acid substitution is likely to alter the three-dimensional structure of the protein, thus making the protein unstable. Taken together, results obtained from this study suggest that hRad18 may regulate the modification status of PCNA by interacting with two different E2s, HHR6A/B and hUbc9, through distinct domains.

Attitude control of spacecraft in low Earth orbits can be achieved by exploiting the torques generated by the geomagnetic field. Recent research has demonstrated that attitude stability of a spacecraft is possible using a linear combination of Euler parameters and angular velocity feedback. The research carried out in this thesis implements a hybrid scheme consisting of magnetic control using on-board dipole moments and a three-axis actuation scheme such as reaction wheels and thrusters. A stability analysis is formulated and analyzed using Floquet and Lyapunov stability theorems.

Elementary particles form hadrons through the strong interaction; one interpretation of a possible hadron bound-state is a hybrid meson which is composed of a quark-antiquark pair and gluonic content. Non-exotic hybrid mesons share spin <i>J</i>, parity <i>P</i> and charge conjugation <i>C</i> quantum numbers with quark-antiquark states while exotic hybrids do not. Aspects of particle physics, strong interactions, and quantum field theory necessary for calculating the correlation function for a hybrid meson will be reviewed. In particular, the perturbative part of the correlation function for a hybrid meson with <i>J</i>^PC=1^-- will be formulated in terms of Feynman rules and diagrams and calculated to next-to-leading order in the light (massless) quark case. Assuming the hybrid current renormalizes multiplicative, the next-to-leading order effects are found to be large, and are potentially important for future determinations of the light-quark non-exotic hybrid meson.

The goal of this research is to understand how three different parameters affect single chain variable fragment (scFv) binding capacity. The parameters that were varied include the number of variable complementarity determining regions (CDRs), the number amino acids used to diversify CDRs, and configuration of the structure. How the parameters affect the binding capacity will be tested using the yeast two hybrid assay against five different protein domains. Eight scFv libraries were generated; the genes expressing the scFvs were constructed and the CDRs were randomized using PCR amplification. Genes expressing scFvs were cloned, using the homologous gap repair mechanism in <i>Saccharomyces cerevisiae</I>. Representative members of scFv libraries were sequenced to confirm correct construction.<p> Library diversity was calculated from the library transformation efficiency. Transformation efficiency refers to the number of cells that grew at the time of transformation of the scFv gene into yeast cells. There were significant differences in the diversity of the scFv libraries, which created difficulty in comparing the library binding capacities. Sequencing the scFv libraries revealed that on average 50% of each library contained correct scFv sequences. The percent of correct sequences within each library was then used to calculate the functional diversity.<p> The yeast two-hybrid assay was used to screen the scFv libraries for interactions and to test binding capacity. The binding capacity of the scFv libraries was tested and compared in five different yeast two-hybrid assays using five protein domains as the targets for each screen. The screening results showed that in all cases cyclic scFv libraries had a statistically significant higher binding capacity than linear scFv libraries despite a diversity bias against the cyclic libraries. There was no clear trend in binding capacity with the other two parameters; however, the four amino acid three CDR libraries dominated over the other libraries in almost every screen.<p> Some of the scFvs isolated from the screens were expressed in <i>E. coli</i> and <i>S. cerevisiae</i>to analyze for proper expression and correct size. All the scFvs that were isolated and analyzed were the correct size and could be purified using a poly histidine tag.<p> Due to its bioaffinity and specificity, scFvs were constructed to profile disease patterns, and to identify potential drug targets. In addition to its original application to health-related studies, scFvs could also be extended to locate potential metabolic bottlenecks, to alter metabolic flux to enhance productivity, and regulate metabolic bionetworks. Industrial microorganisms are generally carrying more than two sets of chromosomes, making it difficult to be genetically engineered when conventional approaches are employed. With the availability of scFvs as reported in this thesis, we are able to design specific scFvs that selectively bind to target proteins, resulting in re-routing of metabolic flux within the microorganism, toward a high productivity of desired product. ScFvs can be applied to industrial microorganisms directly, leading to the development of new fermentation processes.

Technology replaces newer technology with improved efficiency. Solar technology is going to draw out a new life to make a green change in the terms of energy. As a result energy from the sunlight is being changed into electric energy by using solar cell. But still its efficiency could not be able to make a sense as a depending energy technology. In order to look up the solution, solar technology is changing rapidly to get maximum output. To take up this new challenge solar technology is trying to change its building component that are used to make solar cell, for example solar cell material, bypass diode system, blocking diode system etc.   Now-a-days, solar energy system is designed as a hybrid system that can make electricity and hot water at the same time. In the hybrid solar system, photovoltaic and solar thermal systems are integrated at the same system and as a result heat and electricity are produced simultaneously at the same area. Solar cells are attached with both top and the bottom side of the module and the collectors are set up inside the module. By using collector inside the module, rejected heat from the solar cell is absorbed by the water that flows through the collectors. But a problem arises at the midday or after midday because the reflector of this system cannot reflect sunlight properly on the bottom side of the module. That’s why shading is occurred on the bottom side which reduce the total electrical output of this system.   To work out this shading problem, a bypass diode is connected in parallel with the group of solar cells. Schottky diodes are being used as bypass diodes inside in the most of the solar cells. Schottky diode forward voltage drop is almost 0.45 Volt which is an important cause of reducing the output power as well as the efficiency of this hybrid system. To solve this problem, new lossless diode is attached inside the hybrid solar system instead of schottky diode which can work with a very low forward voltage drop roughly 50mV at 10amp.   To make a comparison between the performance of PVT system with the schottky diode and the new lossless diode, many data has been collected from the outdoor test. After getting the output result, it is clear that the output power and efficiency is going to be changed for using the new lossless diode. For using the lossless diode, the efficiency of the bottom side of the module was increased by 0.31 %.

The cellular industry is undergoing a major paradigm shift from voice-centric, structured homogeneous networks to a more data-driven, distributed and heterogeneous architecture. One of the more promising trends emerging from this cellular revolution is femtocells. Femtocells are primarily viewed as a cost-effective way to improve both capacity and indoor coverage, and they enable offloading data-traffic from macrocell network. However, efficient interference management in co-channel deployment of femtocells remains a challenge. Decentralized strategies such as femtocell access control have been identified as an effective means to mitigate cross-tier interference in two-tier networks. Femtocells can be configured to be either open access or closed access. Prior work on access control schemes show that, in the absence of any coordination between the two tiers in terms of power control and user scheduling, closed access is the preferred approach at high user densities. Present methods suggest that in the case of orthogonal multiple access schemes like TDMA/OFDMA, femtocell access control should be adaptive according to the estimated cellular user density. The approach we follow, in this work, is to adopt an open access policy at the femtocell access points with a cap on the maximum number of users allowed on a femtocell. This ensures the femto owner retains a significant portion of the femtocell resources. We design an iterative algorithm for hybrid access control for femtocells that integrates the problems of uplink power control and base station assignment. This algorithm implicitly adapts the femtocell access method to the current user density. The distributed power control algorithm, which is based on Yates' work on standard interference functions, enables users to overcome the interference in the system and satisfy their minimum QoS requirements. The optimal allocation of femtocell resources is incorporated into the access control algorithm through a constrained sum-rate maximization to protect the femto owner from starvation at high user densities. The performance of a two-tier OFDMA femtocell network is then evaluated under the proposed access scheme from a home owner viewpoint, and network operator perspective. System-level simulations show that the proposed access control method can provide a rate gain of nearly 52% for cellular users, compared to closed access, at high user densities and under moderate-to-dense deployment of femtocells. At the same time, the femto owner is prevented from going into outage and only experiences a negligible rate loss. The results obtained establish the quantitative performance advantage of using hybrid access at femtocells with power control at high user densities. The convergence properties of the proposed iterative hybrid access control algorithm are also investigated by varying the user density and the mean number of femto access points in the network. It is shown that for a given system model, the algorithm converges quickly within thirty iterations, provided a feasible solution exists.

Multifunctional hybrid composites are proposed as novel solutions to meet the demands in various industrial applications ranging from aerospace to biomedicine. The combination of carbon fibers and/or fabric, metal foil and carbon nanotubes are utilized to develop such composites. This study focuses on processing of and fracture toughness characterization of the carbon fiber reinforced polymer matrix composites (PMC) and the CNT modified interface between PMC and a metal foil. The laminate fabrication process using H-VARTM, and the mode I interlaminar fracture toughness via double cantilever beam (DCB) tests at both room temperature and high temperature are conducted. The cross-sections and fracture surfaces of the panels are characterized using optical and scanning electron microscopes to verify the existence of CNTs at the interface before and after fracture tests. The experimental results reveal that CNT’s improve bonding at the hybrid interfaces. Computational models are developed to assist the interpretation of experimental results and further investigate damage modes. In this work, analytical solutions to compute the total strain energy release rate as well as mode I and mode II strain energy release rates of asymmetric configurations layups are utilized. Finite element models are developed in which the virtual crack closure technique is adopted to calculate strain energy release rates and investigate the degree and effect of mode-mixity. Results from analytical solutions agree well with each other and with results obtained from finite element models.