L'image de la ville de Bénarès (Kāśī, Varanasi) est liée à son ensemble de ghāṭ – berges en forme de gradins – qui se déploie de façon monumentale sur la rive concave d'un méandre du Gange. L'aménagement de ce front d'eau s'inscrit dans un contexte culturel particulier qui nécessite un rapport de proximité avec l'eau du fleuve. La construction et le développement de ce front d'eau dans le temps et la manière composite dont les éléments architecturaux s'y sont intégrés, interrogent aujourd'hui cette grande unité urbaine qui forme un espace public de plus de 6 km de long. L'objectif de cette thèse est de comprendre les éléments qui ont concouru à la naissance et au développement de ce front. La forme architecturale et urbaine du ghāṭ est considérée comme un dispositif construit modulable adapté à un milieu fluvial, à une topographie et à des pratiques liées à la culture du lieu, qui facilite l'accès à l'eau quel que soit son niveau. M'appuyant sur des images anciennes, sur une série d'enquêtes de terrain et de relevés architecturaux, ainsi que sur des plans de travaux officiels, j'étudie les techniques de construction face aux contraintes de l'eau, j'observe et j'analyse le dialogue de cette forme de berge avec la géographie et le paysage urbain pour en mieux approcher son architecture et j'explique la matérialisation du ghāṭ par les pratiques diverses qui s'y déploient et les parcours symboliques qui y font référence. La relation toute particulière que la ville entretient avec son fleuve par l'architecture des ghāṭ explique son potentiel urbanistique inhérent à son développement dans le temps et le long de la berge / The image of Benares (Kāśī, Varanasi) is closely associated to the architecture of its riverfront composed of ghats – steps and terraces – that stretch out in a monumental way on the concave bank of a meander formed by the river Ganges. The expansion of this riverfront has to be understood in relation to a cultural tradition that demands a close proximity to the waters of the river. The construction and development of this riverfront in time and the various ways in which each architectural element is integrated with the river bank, calls into question its impressive urban unity that forms a public space extending more than 6 km. The aim of this thesis is to identify the elements that have contributed to the origin and the development of this front. The architectural and urban form of the ghat is considered as a constructed flexible device adapted to a specific river environment, a characteristic topography and to practices linked to the culture of the place, which allows for easy access to the varying levels of the river's water level. Based on pictorial archives, interviews, measure drawings done on site and on official planners' drawings, I examine the techniques of hydraulic constructions best adapted to counter the river's currents, I observe and analyze the relations between the riverbank, the geography and the urban landscape so as to better approach the ghats' architecture and I explain the materialization of the ghat through the diverse practices and the symbolic trajectories that surround it. The particular relation that the city entertains with its river, through the architecture of ghats, explains its urban potential inherent to its development in time and space i.e. along its river bank

Doctor of Philosophy / Department of Agronomy / P.V. Vara Prasad / The pathway for the movement of water through plants, from the soil matrix to the atmosphere, constitutes the hydraulic architecture of a plant. The linkage between the hydraulic architecture of woody plants and drought tolerance has received considerable attention, but much less work has been done on grasses. I investigated the linkage between the hydraulic architecture of grasses to physiological patterns of water use across a range of species and conditions. The rate of stomatal conductance (g[subscript]s) and photosynthesis (A) increased acropetally along the leaves of 5 grass species, which is a unique feature of this growth form. The internal structure of leaves also changed acropetally in order to minimize the pressure gradient across the mesophyll that would otherwise occur as a result of increasing g[subscript]s. The resistance to water movement through the mesophyll represented 80-90% of leaf resistance in six genotypes of Sorghum bicolor L. (Moench). This resistance was most important in controlling g[subscript]s and A when water was readily available, but as soil-moisture decreased it was the efficient transport of water through the xylem that was most important in maintaining plant function. I also investigated the relationship between hydraulic architecture and stomatal responses of grasses to increasing Vapor Pressure Deficit (D). Grasses with a larger proportion of their hydraulic resistance within the xylem were less sensitive to increasing D and plants with high root conductance maintained higher rates of gas exchange D increased. Finally, I investigated the tolerance of grasses to extreme drought events to test if there was a trade-off between drought tolerance and growth in grasses. Plants with drought tolerant leaf traits typically sacrificed the ability to move water efficiently through their leaves. Having drought tolerant leaves did not limit the plants ability to have high rates of gas exchange, and, in fact, the most drought tolerant plants had the high rates of g[subscript]s when expressed on a mass basis. Leaf-level drought tolerance did contribute to species’ occurrence, as the drought intolerant species I studied are not commonly found in low precipitation systems. The results presented here highlight the importance of studying the hydraulic architecture of plants to provide a better understanding of what controls plant function across a range of environmental conditions.

<p>Plants respond to changes in their local environment and, at the same time, influence the environment at a global scale. The molecular and physiological mechanisms regulating this interaction are not completely understood and this limits our capacity to predict the response of vegetation to future environmental changes. This dissertation combined tools from genomics, physiology, and ecology to examine the response of plants to environmental change. Specifically, it focused on processes affecting carbon and water exchange in forest trees because (1) trees are long-lived species that might face repeated environmental challenges; (2) relatively little information exists about the genes and the molecular mechanisms regulating structural and physiological traits in adult, long-lived woody plants; and (3) forest trees exchange a significant amount of carbon and water with the atmosphere and are therefore major players in the global carbon and water cycles. </p><p>Water flux through forests depends both on environmental conditions (e.g., soil moisture) and on the hydraulic architecture of individual trees. Resistance to xylem cavitation is an important hydraulic trait that is often associated with drought tolerance but potentially at the cost of reduced carbon uptake. The second chapter of this dissertation evaluated the variation in resistance to xylem cavitation, hydraulic conductivity, wood anatomy traits, and leaf gas exchange across 14 co-occurring temperate tree species including both angiosperms and gymnosperms. The relationship between vulnerability to cavitation (ψ₅₀) and hydraulic conductivity within specific organs (i.e. stems and roots) was not significant when considering the phylogenetic association between species. However, even after phylogenetic correction, photosynthetic carbon uptake (A) was positively correlated with both stem and root ψ₅₀, and stomatal conductance (g_s) was strongly correlated with root ψ₅₀ . These results suggest that there is a trade-off between vulnerability to cavitation and water transport capacity at the whole-plant level, and that this functional relationship reflects an adaptive response to the environment. </p><p>Forests are an important component of the global carbon cycle that can be directly impacted by a rise in atmospheric CO₂ concentration.. The third chapter of this dissertation investigated the effects of long-term exposure to elevated CO2 on the gene expression of mature, field-grown loblolly pine trees. Using cDNA microarrays, I compared the expression of 1784 pine transcripts in trees growing under ambient and those under elevated CO₂ at monthly intervals throughout a growing season. Overall, more genes were upregulated than downregulated by elevated CO₂, although the total number of genes differentially expressed varied throughout the season. The pattern of increasing number of differentially expressed genes until the peak of the growing season (July and August) followed by a decrease in that number, matched the seasonal trend of tree growth and photosynthetic response to elevated CO₂ in this species. The seasonal trend also reflected the interaction among multiple abiotic factors intrinsic to field conditions and emphasized the relevance of evaluating the role of genes in their natural environment. Genes consistently upregulated by elevated CO₂ were functionally associated with environmental sensing, cellular signaling, and carbon metabolism, in particular the degradation of carbohydrates through respiration. An increase in carbohydrates degradation is particularly relevant in the context of carbon balance of forest trees because of the potential for enhanced leaf and tree respiration leading to a reduced sink capacity for CO₂. </p><p>Loblolly pine produces several flushes of needles throughout the year each with an average lifespan of 19 months. Each year, two age classes of needles contribute to the annual carbon sequestration of the loblolly pine forest. To address the impact of leaf age on the effects of elevated CO₂ in carbon metabolism regulation, I compared the gene expression profiles from trees under ambient and elevated CO₂ conditions in two needle cohorts: one-year-old and current-year. Differential expression under elevated CO₂ was seven times more frequent in current-year than in one-year-old needles. Despite differences in magnitude, many of the patterns within specific groups of genes were similar across age classes. For instance, there was a trend for downregulation of genes involved in the light-reactions of photosynthesis and those in photorespiration in both age classes, while genes associated with dark respiration were largely upregulated by elevated CO₂ in both cases. The difference between the two cohorts was particularly evident in the group of genes related to energy production (ATP synthesis) and the group associated with carbon partitioning (sucrose and starch metabolism). Because sucrose and starch metabolism categories included many genes known to be important regulators of gene expression and plant physiological processes, this suggests that this stage of carbon metabolism might be an important control point in age-dependent foliar responses to elevated CO₂.</p><p>This dissertation examined both structural and physiological components of plant water and carbon relations (Chapter 2) across different biological scales of organization (whole-plant level in Chapter 2; gene-level response to ecosystem-level changes in Chapters 3 and 4) and reflecting adjustments at distinct temporal scales (life-span of the organism vs. evolutionary selection of traits). An integrative approach was used to advance our understanding of how plants acclimate and adapt to their environment, and to provide a mechanistic framework for predictive models of plant response to environmental change. </p> / Dissertation

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Orientadores: Fernando Roberto Martins, Rafael Silva Oliveira / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-22T21:00:03Z (GMT). No. of bitstreams: 1 Dias_ArildodeSouza_D.pdf: 101885895 bytes, checksum: 1d2644f73311bbbaee1346d9d6c59e1b (MD5) Previous issue date: 2013 / Resumo: Compreender como espécies de plantas diferenciam-se em relação a atributos funcionais para aquisição e utilização de recursos, e se a relação entre esses atributos varia em função de diferenças ambientais, como pluviosidade total e sazonalidade, e uma forma de construir uma ecologia mais preditiva no contexto das mudanças climáticas globais. Nesta tese investigamos como arvores e lianas se diferenciam em relação a atributos funcionais da folha dos ramos. Analisamos-nos se o relacionamento entre esses atributos diferia considerando a variação de pluviosidade e sazonalidade de duas fitofiosionomias da Floresta Atlântica, a floresta ombrofila densa (FO) e a floresta estacional semedicidua (FS). Encontramos diferenças não são na amplitude, mas também na forca do relacionamento entre os atributos funcionais foliares estudados. Essas diferenças parecem ser determinadas pela diferença ambiental entre as duas florestas estudadas e por características intrínsecas do habito de crescimento de lianas e arvores. A anatomia do xilema das espécies de lianas e arvores da FO difere em relação à das espécies da FS, mesmo quando consideramos o parentesco filogenético. Por investir pouco em tecidos de sustentação esperava-se menor conteúdo de lignina nos ramos de lianas, entretanto nosso resulto foi o oposto. Encontramos a razão S/G dos monômeros syringil e guaiacil que compõe a lignina menor que um para duas espécies de lianas. Indicando que ha maior deposição de lignina em vasos de xilema do que em fibras. Esse fato pode estar relacionado à maior eficiência em condutividade hidráulica de lianas ou a requerimentos biomecânicos específicos dos ramos de lianas. Em particular, ressalta-se o fato de que lianas foram mais eficientes em captar luz (menor MFA) e tiveram maior condutividade hidráulica potencial (Kp) do que arvores na FS. A maior competitividade de lianas frente arvores apenas na FS tem implicação importante no cenário de mudanças climáticas / Abstract: Understanding how plant species differentiated in functional traits for resources acquisition and use, and if the relationships among those traits vary in according with environmental characteristics such as rainfall and seasonality, could lead to a more predictive science in the context of global change. Here we investigate how trees and lianas differ in leaf and xylem anatomical traits related to water transport and hydraulic architecture. We analyze the relationships among those traits taking into account the variation in rainfall and seasonality between two types of Atlantic Forest, semediciduous seasonal forest (SF) and the dense ombrophilous forest (OF). We found differences not only in range, but in the strength of the relationship among leaf functional traits, which in turn could be related to environmental differences between the two forests studied. The xylem anatomy of lianas and tree species of OF differ compared to species of SF despite taking to account the phylogenetic relatedness. Since lianas have low investment in support tissues we expected lower lignin content in the branches of lianas. However, we found the opposite pattern. Another unexpected result found for two liana species was the ratio to the monomers syringyl and guayacil present at lignin (S/G) lower than one. This result indicates that there is more deposition of lignin in xylem vessel walls than fibers, what in turn would be linked to greater efficiency in hydraulic conductivity of lianas or to specific biomechanical requirements of the branches of lianas. In particular, we highlighted the fact that liana species are more efficient in light acquisition (lower LMA) and had higher hydraulic conductivity (Kp) relative to trees just in the SF. These lianas competitive advantages over trees have important implications in the context of climatic changes / Doutorado / Biologia Vegetal / Doutor em Biologia Vegetal

Africa's miombo woodlands constitute one of the most important dry tropical forests on earth, yet the hydraulic function of these woodlands remains poorly researched. Given the current predictions of increased aridity by the end of this century in the miombo ecoregion, understanding the likely response of miombo woodlands tree species to water stress is crucial in planning adaptation strategies. Predicting the response of miombo woodlands to future climate trends is hampered by a lack of knowledge on the physiology of the common miombo woodlands tree species. In particular, plant-water relations for this woodlands type are not well understood. An understanding of plant-water relations for this woodlands type will provide insights into how water limits tree species distribution in this ecosystem. This will also improve our prediction model on the likely response of this ecosystem to predicted climate change. For this reason, the overall objective of this research was to evaluate the hydraulic architecture and xylem vulnerability to cavitation for nine principal miombo woodlands tree species differing in drought tolerance ability and habitat preference. This was achieved by; examining the hydraulic properties and evaluating the extent to which each hydraulic design was vulnerable to water stress-induced xylem cavitation; investigating how seasonal changes in plant-water relations influences seasonal patterns of leaf display and; analyzing the relationship between stem hydraulic supply and leaf functional traits related to drought tolerance ability. This research has found that drought-intolerant tree species with mesic specialization have more efficient stem hydraulic systems than co-occurring habitat broad ranging species. Broad ranging tree species attain wider habitat distribution by adjusting their hydraulic supply in response to changing ecosystem water availability. The finding that hydraulic properties differ significantly between tree species with contrasting habitat preference suggests that tree hydraulic design may have some adaptive ecological role in influencing species habitat preferences in miombo woodlands. The evaluation of xylem vulnerability to cavitation revealed that mesic specialized tree species were more vulnerable to water stress-induced cavitation than habitat broad ranging tree species. Vulnerability to cavitation in individuals from the same broad-ranging species growing in contrasting habitats showed only marginal and statistically insignificant (P > 0.05) differences between wet and dry sites. In the investigation of the influence of seasonal changes in stem water relations on seasonal leaf display, seasonal rhythms in stem water status were found to exert significant controls on leaf phenology. Mesic specialists had strong stem water controls throughout the year in comparison to broad ranging tree species. An analysis of the relationship between stem hydraulic supply and leaf functional traits suggests that stem hydraulic supply constrains leaf biomass allocation patterns among miombo tree species. Mesic specialists tend to invest more in leaf longevity than broad ranging tree species. This thesis has uncovered some interesting relationships between plant-water-relations and the distribution of miombo woodlands tree species. These results lead to the conclusion that in an event of increased ecosystem drying under future climate trends, tree species with mesic specialisation are at a greater risk of experiencing cavitation related species mortality than broad ranging ones.

Cette thèse visait à caractériser l’architecture hydraulique du xylème chez les hybrides de peuplier Populusdeltoides × P. nigra et à juger de ses relations avec le fonctionnement hydrique et carboné de l’arbre en conditions d’irrigation contrastées. A cette fin, huit génotypes se distinguant par leur discrimination isotopique vis-à-vis du carbone 13 ont été cultivés en pépinière. Nos travaux ont permis de mettre en évidence d’importantes variations entre génotypes pour les caractéristiques anatomiques du xylème, l’efficience hydraulique de la tige et de la plante entière ainsi que la résistance à la cavitation. Nos travaux démontrent également que l’anatomie et la résistance à la cavitation du xylème s’ajustent aux conditions hydriques du milieu de façon génotype-dépendante. En condition hydrique non limitante, nous avons mis en évidence un compromis entre l’efficience hydraulique et la résistance à la cavitation ou le potentiel de croissance. Ces deux compromis expliquent la relation positive observée entre la résistance à la cavitationet le potentiel de croissance. En condition hydrique limitante, cette relation n’était toutefois plus observable. Aucune relation n’a pu être identifiée entre les propriétés hydrauliques et l’efficience d’utilisation de l’eau. Ces travaux suggèrent que certaines relations couramment observées à l’échelle inter-spécifique ne sont pas nécessairement applicables à des échelles d’étude plus réduites. Ce travail ouvre des perspectives sur le plan fondamental pour l’identification du déterminisme moléculaire à l’origine de la plasticité structurale observée et sur le plan appliqué, pour la création variétale. / This work aimed at characterizing xylem hydraulic architecture and at describing its relationships with whole plant water and carbon relations among Populus deltoides × P. nigra hybrids under contrasting water regimes. Eight genotypes differing in carbon isotope discrimination were grown in the field in a common garden test. Significant variations were observed between genotypes for all xylem anatomical characteristics, stem or whole-plant hydraulic efficiency and xylem resistance to cavitation. Drought-induced acclimation was observed for xylem structural features and xylem resistance to cavitation, but in a genotype-dependant manner. Under optimal irrigation, a trade-off was observed between hydraulic efficiency and xylem resistance to cavitation or growth performance. These two trade-offs translated into an uncommon positive relationship between xylem resistance to cavitation and growth performance. Under water deficit,this relationship broke down. No relationship could be detected between xylem hydraulics and water-use efficiency estimates. Our results suggest in part that several common relationships that are observed at the between-species level may not necessarily hold true at narrower scales. Further, this work opens up prospects both for identifying the molecular basis of xylem structural acclimation and for breeding strategies.

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