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Exercise-induced alterations in immunoglobulin (IgA, IgG, IgM) levels in cancer versus non-cancer patientsSellers, Lisa K. January 2008 (has links)
Thesis (M.S.)--Ball State University, 2008. / Title from PDF t.p. (viewed on Sept. 09, 2009). Includes bibliographical references (p. 41-43).
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Análise dos níveis de imunoglobulinas, interleucinas e colonização bacteriana em amostras salivares nos primeiros meses de vida em nascidos a termo e pré-termo / Analysis of levels of immunoglobulins, interleukins, and bacterial colonization in saliva samples in the first months of life in infants born fullterm and pretermSesso, Maria Lúcia Talarico 14 December 2012 (has links)
O sistema imune de mucosas representa uma importante ferramenta de defesa contra a colonização da cavidade oral, especialmente pela presença das imunoglobulinas salivares no início da vida. Além das imunoglobulinas, as citocinas salivares também possuem funções importantes, pois utilizam células linfoides para amplificar ou deprimir a resposta inflamatória e podem ser consideradas biomarcadores proteicos. No entanto, pouco se sabe sobre a ontogenia dos componentes do sistema imune de mucosas em recém-nascidos a termo (AT) e especialmente em pré-termo (PT, com gestação inferior a 37 semanas) que reconhecidamente apresentam imaturidade imunológica. O objetivo do presente estudo foi o de analisar os níveis de imunoglobulinas (Ig) A (IgA) e M (IgM), citocinas: interleucinas (IL) IL-6, IL-10, IL-12 e interferon (IFN) (IFN-?) e 20 tipos distintos de bactérias orais em amostras de salivas de crianças ao nascer (T0) e após 3 meses de vida (T3) em PT e AT. Para isto, 123 amostras de saliva (70 AT e 53 PT) foram coletadas e os níveis de IgA e IgM foram quantificadas por ensaios ELISA. Em um subgrupo de 50 amostras (25 AT e 25 PT) foram analisadas a presença de 20 tipos diferentes de espécies bacterianas através de Checkerboard e os níveis de citocinas através de ensaio Luminex®. Dados socioeconômicos e do nascimento foram avaliados através de questionários. Os resultados do Checkerboard mostraram que nenhuma criança apresentou níveis detectáveis das bactérias testadas. Houve diferenças estatisticamente significantes (p<0.05) nos níveis de IgA em T0, sendo que PT apresentaram níveis inferiores aos AT, o que não aconteceu em T3. Diferentemente, não houve diferenças nos níveis de IgM entre os grupos e visitas (p>0.05). A análise prospectiva mostrou que houve um aumento dos níveis de IgA de T0 para T3 em ambos os grupos. Quanto à citocinas, houve diferenças somente em T0 entre PT e AT, nos níveis de IL-6 e IL10 que foram significantemente superiores em PT (p<0.05). Desta maneira, os dados sugerem que a prematuridade pode levar a algumas diferenças no status da resposta imunológica de mucosas no nascimento o que não acontece após 3 meses. / The mucosal immune system through the secretion of salivary immunoglobulins represent an important line of defense against initial acquisition and bacteria colonization of the oral cavity especially early in life. Cytokines are protein signaling molecules that lymphoid cells use to amplify or down regulate the inflammatory response. Little is known about the ontogeny of the mucosal immune system and bacterial colonization in saliva from neonates at fullterm (FT) and especially preterm (PT, with less than 37 weeks gestation), known to have an immunological immaturity. The aim of this study was to analyze the levels of immunoglobulin A (IgA) and M (IgM), four cytokines: interleukin (IL) IL-6, IL-10, IL-12 and interferon gamma (IFN-?) and 20 types of bacteria in saliva samples at birth (T0) and after 3 months (T3) in PT and FT. For this, 123 saliva samples (70 FT and 53 PT) were collected and levels of IgA and IgM were quantified by ELISA assays. In a subgroup of 50 samples (25 FT and 25 PT) was analyzed for the presence of 20 different types of bacterial species using checkerboard and the levels of cytokines through Luminex® assay. Economic data and birth partners were assessed through questionnaires. The results the Checkerboard showed that no child had detectable levels of bacteria. There were statistically significant (p <0.05) in IgA levels at T0, and PT showed lower levels than FT, which has not happened in T3. In contrast no differences in levels of IgM and visits between the groups (p> 0.05). The prospective analysis showed that there was an increase of IgA levels from T0 to T3 in both groups. As for cytokines, there were only differences at T0 between PT and AT, in IL-6 and IL-10 that were significantly higher in PT (p <0.05). Thus, the data suggest that prematurity may lead to differences in the status of the mucosal immune response at birth but not at 3 months of age.
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Análise dos níveis de imunoglobulinas, interleucinas e colonização bacteriana em amostras salivares nos primeiros meses de vida em nascidos a termo e pré-termo / Analysis of levels of immunoglobulins, interleukins, and bacterial colonization in saliva samples in the first months of life in infants born fullterm and pretermMaria Lúcia Talarico Sesso 14 December 2012 (has links)
O sistema imune de mucosas representa uma importante ferramenta de defesa contra a colonização da cavidade oral, especialmente pela presença das imunoglobulinas salivares no início da vida. Além das imunoglobulinas, as citocinas salivares também possuem funções importantes, pois utilizam células linfoides para amplificar ou deprimir a resposta inflamatória e podem ser consideradas biomarcadores proteicos. No entanto, pouco se sabe sobre a ontogenia dos componentes do sistema imune de mucosas em recém-nascidos a termo (AT) e especialmente em pré-termo (PT, com gestação inferior a 37 semanas) que reconhecidamente apresentam imaturidade imunológica. O objetivo do presente estudo foi o de analisar os níveis de imunoglobulinas (Ig) A (IgA) e M (IgM), citocinas: interleucinas (IL) IL-6, IL-10, IL-12 e interferon (IFN) (IFN-?) e 20 tipos distintos de bactérias orais em amostras de salivas de crianças ao nascer (T0) e após 3 meses de vida (T3) em PT e AT. Para isto, 123 amostras de saliva (70 AT e 53 PT) foram coletadas e os níveis de IgA e IgM foram quantificadas por ensaios ELISA. Em um subgrupo de 50 amostras (25 AT e 25 PT) foram analisadas a presença de 20 tipos diferentes de espécies bacterianas através de Checkerboard e os níveis de citocinas através de ensaio Luminex®. Dados socioeconômicos e do nascimento foram avaliados através de questionários. Os resultados do Checkerboard mostraram que nenhuma criança apresentou níveis detectáveis das bactérias testadas. Houve diferenças estatisticamente significantes (p<0.05) nos níveis de IgA em T0, sendo que PT apresentaram níveis inferiores aos AT, o que não aconteceu em T3. Diferentemente, não houve diferenças nos níveis de IgM entre os grupos e visitas (p>0.05). A análise prospectiva mostrou que houve um aumento dos níveis de IgA de T0 para T3 em ambos os grupos. Quanto à citocinas, houve diferenças somente em T0 entre PT e AT, nos níveis de IL-6 e IL10 que foram significantemente superiores em PT (p<0.05). Desta maneira, os dados sugerem que a prematuridade pode levar a algumas diferenças no status da resposta imunológica de mucosas no nascimento o que não acontece após 3 meses. / The mucosal immune system through the secretion of salivary immunoglobulins represent an important line of defense against initial acquisition and bacteria colonization of the oral cavity especially early in life. Cytokines are protein signaling molecules that lymphoid cells use to amplify or down regulate the inflammatory response. Little is known about the ontogeny of the mucosal immune system and bacterial colonization in saliva from neonates at fullterm (FT) and especially preterm (PT, with less than 37 weeks gestation), known to have an immunological immaturity. The aim of this study was to analyze the levels of immunoglobulin A (IgA) and M (IgM), four cytokines: interleukin (IL) IL-6, IL-10, IL-12 and interferon gamma (IFN-?) and 20 types of bacteria in saliva samples at birth (T0) and after 3 months (T3) in PT and FT. For this, 123 saliva samples (70 FT and 53 PT) were collected and levels of IgA and IgM were quantified by ELISA assays. In a subgroup of 50 samples (25 FT and 25 PT) was analyzed for the presence of 20 different types of bacterial species using checkerboard and the levels of cytokines through Luminex® assay. Economic data and birth partners were assessed through questionnaires. The results the Checkerboard showed that no child had detectable levels of bacteria. There were statistically significant (p <0.05) in IgA levels at T0, and PT showed lower levels than FT, which has not happened in T3. In contrast no differences in levels of IgM and visits between the groups (p> 0.05). The prospective analysis showed that there was an increase of IgA levels from T0 to T3 in both groups. As for cytokines, there were only differences at T0 between PT and AT, in IL-6 and IL-10 that were significantly higher in PT (p <0.05). Thus, the data suggest that prematurity may lead to differences in the status of the mucosal immune response at birth but not at 3 months of age.
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Optimisation of dengue diagnostic tools in order to increase the knowledge of the pathogenesisLindegren, Gunnel. January 2008 (has links)
Lic.-avh. (sammanfattning) Stockholm : Karolinska institutet, 2008.
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Exercise-induced alterations in immunoglobulin (IgA, IgG, IgM) levels in cancer versus non-cancer patients / Exercise induced alterations in immunoglobulin (IgA, IgG, IgM) levels in cancer versus non-cancer patientsSellers, Lisa K. January 2008 (has links)
A suppressed immune system is a complicating health factor in cancer patients that keeps them from achieving the highest quality of life possible. Moderate exercise is thought to boost the immune system in cancer patients. The aim of this project was to determine the effects of an eight week aerobic exercise program on the mucosal immune system of cancer survivors compared to non-cancer patients. Our hypothesis was that the immune system of the cancer patients would positively respond to a moderate exercise program, specifically increasing antibody production. To examine our hypothesis, five cancer and six non-cancer patients undertook a supervised moderate aerobic exercise program at the University of Northern Colorado. The subjects performed an incremental peak treadmill test to exhaustion at the start of the program and after 8 weeks of training. Saliva samples were taken at specific times for each peak exercise test: prior to testing, immediately after testing, and 30 minutes post-test. Enzyme-Linked ImmunoSorbent Assays (ELISA) were performed at Ball State University to analyze the levels of immunoglobulins (IgA, IgG, IgM) in saliva samples of cancer and non-cancer patients. Our findings demonstrated there was a significant increase in IgG after 8 weeks of moderate exercise in non-cancer patients 30 minutes after the treadmill test. A significant increase was also seen in salivary IgA levels after 8 weeks of moderate exercise in cancer patients 30 minutes after the treadmill test was administered, supporting our hypothesis that exercise enhances immune function. Eight weeks of moderate exercise has been shown to enhance immune function demonstrated by the increase of IgA and IgG levels in saliva. / Department of Biology
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Regulation of marginal zone B cell migration in the primary IgM antibody response /Rubtsov, Anatoly V. January 2007 (has links)
Thesis (Ph.D. in Immunology) -- University of Colorado Denver, 2007. / Typescript. Includes bibliographical references (leaves 146-169). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;
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T dependent B cell help in cattle : immunoregulatory function of interleukin-4 and CD40-CD40L interactions /Hirano, Ayumi, January 1997 (has links)
Thesis (Ph. D.)--University of Missouri--Columbia, 1997. / "August 1997." Typescript. Vita. Includes bibliographical references (leaves 82-96). Also available on the Internet.
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T dependent B cell help in cattle immunoregulatory function of interleukin-4 and CD40-CD40L interactions /Hirano, Ayumi, January 1997 (has links)
Thesis (Ph. D.)--University of Missouri--Columbia, 1997. / Typescript. Vita. Includes bibliographical references (leaves: 82-96). Also available on the Internet.
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Via anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansi / Cholinergic anti-inflammatory pathway and acute phase proteins in trypanotolerance of rabbits infected by Trypanosoma evansiCosta, Marcio Machado 26 April 2013 (has links)
Conselho Nacional de Desenvolvimento Científico e Tecnológico / Trypanosomiasis is a disease of worldwide distribution which in Brazil is called "mal das
cadeiras", owing the clinical signs shown by horses, which are the major species affected. The
disease is caused by the protozoan Trypanosoma evansi, characterized by clinical signs such
as weight loss, pale mucous membranes, swelling of the eyelid and vulva. Rabbits have been
reported as a resistant species to T. evansi, however, is not yet known how immune aspects,
such as innate immune and cholinergic system, behave in this species, not even as this
inflammations pathways influence in rabbits's trypanotolerance. Thus, the aim of this study
was to investigate the inflammatory response and cholinergic anti-inflammatory pathway and
its relation to trypanotolerance in rabbits infected with T. evansi. For this, twelve female adult
New Zealand rabbits, weighing 3.6 4.5 kg were used in this study. The animals were divided
into two groups, a control group and an infected group, both with six animals. The rabbits
belonging to the infected group received, intraperitoneally, 0.5 ml of rat blood containing 108
parasites per animal, while the control group received physiological solution by the same
route. The experimental period reached 118 days. Blood was collected on days 0, 2, 7, 12, 27,
42, 57, 87, 102, and 118, for to determine the cholinesterases, and days 0, 5, 20, 35, 65, 95
and 118, for to evaluate blood count, total protein, serum proteinogram, immunoglobulins and
acute phase proteins. There was an increase in the activity of butyrylcholinesterase (BChE), at
7th day PI, and in the activity of acetylcholinesterase (AChE), in 27th day PI. Furthermore, the
infected group showed an increase in total protein and the fractions alpha, beta and gamma
globulins along of experimental period. The reduction in albumin and hematocrit were
observed in precise periods of experimental infection, as well as the increase in
immunoglobulin G. Infection with T. evansi stimulated the production of acute phase
proteins, such as C-reactive protein, haptoglobin, α-2 macroglobulin, being observed increase
in immunoglobulin M (IgM) throughout the experimental period (118 days post-infection).
From these results, it is concluded that the cholinergic pathway had influence the
inflammatory response, through the action of AChE and BChE in the regulation of
concentrations of acetylcholine, resulting in increased concentrations of cytokines and,
consequently, in the production phase protein acute. The increase in IgM, associated with
increased C-reactive protein and haptoglobin, suggests the involvement of these proteins in
host defense against flagellated, with possible participation in trypanotolerance of rabbits
infected with T. evansi. / A tripanossomose é uma enfermidade de distribuição mundial que, no Brasil, é denominada
mal das cadeiras , em função dos sinais clínicos apresentados pelos equinos, principal
espécie afetada. A doença é causada pelo protozoário Trypanosoma evansi, sendo
caracterizada por sinais clínicos como perda de peso, mucosas pálidas, edema de pálpebra e
vulva. Coelhos têm sido relatados como uma espécie resistente ao T. evansi, contudo, ainda
não se sabe de que modo aspectos imunitários, como a imunidade inata e o sistema
colinérgico, comportam-se nessa espécie, nem mesmo como essas vias da inflamação
influenciam a tripanotolerância de coelhos. Assim, o objetivo deste trabalho foi investigar a
resposta inflamatória e a via anti-inflamatória colinérgica e sua relação na tripanotolerância
em coelhos infectados experimentalmente pelo T. evansi. Para tanto, foram utilizados 12
coelhos adultos, fêmeas, da raça Nova Zelândia, com peso corporal entre 3,6 a 4,5 Kg,
divididos em dois grupos, um grupo controle e um grupo infectado, ambos com seis animais.
Os coelhos pertencentes ao grupo infectado receberam, pela via intraperitoneal, 0,5 mL de
sangue de rato contendo 108 tripanossomas por animal, enquanto que o grupo controle
recebeu, pela mesma via, solução fisiológica. O período experimental foi de 118 dias, sendo o
sangue coletado nos dias 0, 2, 7, 12, 27, 42, 57, 87, 102, e 118, para determinar as
colinesterases, e nos dias 0, 5, 20, 35, 65, 95 e 118, para avaliar hemograma, proteínas totais,
proteinograma sérico, imunoglobulinas e proteínas de fase aguda. Foi observado aumento na
atividade da butirilcolinesterase (BChE) no 7° dia PI e da atividade da acetilcolinesterase
(AChE) no 27° dia PI. Além disso, o grupo infectado apresentou aumento nas proteínas totais,
bem como nas frações alfa, beta e gama globulinas ao longo do período experimental. A
redução na albumina e no hematócrito foram observados em períodos pontuais da infecção
experimental, bem como o aumento na imunoglobulina G. A infecção pelo T. evansi
estimulou a produção de proteínas de fase aguda como a proteína C-reativa, haptoglobina, α-2
macroglobulina, sendo observado um aumento na imunoglobulina M (IgM) em todo o
período experimental (118 dias pós-infecção). A partir desses resultados, conclui-se que a via
colinérgica pode ter influência na resposta inflamatória, através da ação da BChE e AChE na
regulação das concentrações de acetilcolina, acarretando em aumento nas concentrações de
citocinas e, consequentemente, na produção de proteínas de fase aguda. A elevação na IgM,
relacionada ao aumento da proteína C-reativa e haptoglobina, sugere o envolvimento dessas
proteínas na defesa do hospedeiro contra o flagelado, e, possivelmente, na tripanotolerância
de coelhos infectados com T. evansi.
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Host genetic factors and antibody responses with potential involvement in the susceptibility to malaria /Israelsson, Elisabeth, January 2008 (has links)
Diss. (sammanfattning) Stockholm : Stockholms universitet, 2008. / Härtill 5 uppsatser.
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