This dissertation describes the development of a new method for increasing the resolution of the current protein microarray technology, down to the single molecule detection level. By using a technique called size-dependent self-assembly, different proteins can be bound to different sized fluorescent nanostructures, and then located on a patterned silicon substrate based on the sized pattern which is closest to the size of the bead diameter.The protein nanoarray was used to detect antibody-antigen binding, specifically anti-mouse IgG binding to mouse IgG. The protein nanoarray is designed with the goal of analyzing rare proteins. However, common proteins, such as IgG, are used in the initial testing of the array functionality. Mouse IgG, representing rare proteins, is conjugated to fluorescent beads and the beads are immobilized on a patterned silicon surface. Then anti-mouse IgG binds to the mouse IgG on the immobilized beads. The binding of the antibody, anti-mouse IgG, to the antigen, mouse IgG is determined by fluorescent signal attenuation.The first objective was to bind charged nanoparticles, conjugated with proteins, to an oppositely charged silicon substrate. Binding of negatively charged gold nanoparticles (AuNP), conjugated with mouse IgG, to a positively charged silicon surface was successful.The second objective was to demonstrate the method of size-dependent self-assembly at the nanometer scale (<100 >nm). Different-sized, carboxylated, fluorescent beads and AuNP, which were conjugated with proteins, were serially added to a patterned polymethyl methacrylate (PMMA) coated silicon surface. Size-dependent self-assembly was successfully demonstrated, down to the nanometer scale.The final objective was to obtain a signal from antibody-antigen binding within the protein array. Conjugated fluorescent beads were bound to e-beam patterns and signal attenuation was measured when the antibodies bound to the conjugated beads. The size-dependent self-assembly is a valuable new method that can be used for the detection and quantification of proteins.

This report investigates the feasibility and accuracy of determining fastener flexibility with 3D FE and representing fasteners in FE load distribution models with simple elements such as springs or beams. A detailed study of 3D models compared to experimental data is followed by a parametric study of different shell modelling techniques. These are evaluated and compared with industry semi-empirical equations. The evaluated 3D models were found to match the experimental values with good precision. Simulations based on these types of 3D models may replace experimental tests. Two different modelling techniques were also evaluated for use in load distribution models. Both were verified to work very well with representing fastener installations in lap-joints using the ABAQUS/Standard solver. Further improvement of one of the models was made through a modification scale factor. Finally, the same modelling technique was verified using the NASTRAN solver. To summarize, it is concluded that: • Detailed 3D-models with material properties defined from stress-strain curves correspond well to experiments and simulations may replace actual flexibility tests. • At mid-surface modelling of the connecting parts, beam elements with a circular cross section as a connector between shell elements is an easy and accurate modelling technique, with the only data input of bolt material and dimension. • Using connector elements is accurate only if the connecting parts are modelled in the same plane, i.e. with no offset. Secondary bending due to offset should only be accounted for once and only once throughout the analysis, and it is already included in the flexibility input.

The objective of this research program is to further evaluate the performance and constructability of reinforced concrete (RC) column-steel beam-slab systems (RCS) for use in low- to mid-rise space frame buildings located in regions of high wind loads and/or moderate seismicity. To better understand these systems, two full scale RCS cruciform specimens were tested under bidirectional quasi-static reversed cyclic loading. The experimental portion of this research program included the construction and testing of two full-scale cruciform specimens with identical overall dimensions but with different joint detailing. The two joint details evaluated were joint cover plates and face bearing plates with localized transverse ties. The construction process was recorded in detail and related to actual field construction practices. The specimens were tested experimentally in quasi-static reversed cyclic loading in both orthogonal loading directions while a constant axial force was applied to the column, to simulate the wind loads in a subassembly of a prototype building. To compliment the experimental work, nonlinear analyses were performed to evaluate the specimen strength and hysteretic degradation parameters for RCS systems. In addition, current recommendations in the literature on the design of RCS joints were used to estimate specimen joint strength and were compared with the experimental findings.

Aim and tasks of the work. The aim of this work is to investigate insufficiently analyzed variants of the electrodynamic super-wide-band slow-wave structures, create their models, improve methods of analysis, analyze properties of the systems and reveal potentiality of the traveling-wave cathode-ray tubes, slow-wave structures. In order to achieve the aim it is necessary: 1. To improve method for evaluation of non-linear distortions in the traveling-wave cathode-ray tubes and reveal possibilities of reduction of non-linear distortions. 2. To create models of the insufficiently analyzed variants of slow-wave structures and reveal properties of the slow-wave structures. 3. To reveal influence of periodical non-homogeneities on properties of slow-wave structures, simulate and reveal influence of transitions to properties of slow-wave structures and traveling-wav cathode-ray tubes. 4. To make investigation of potentiality of slow-wave structures and traveling-wave cathode-ray tubes and select variants of slow-wave structures that can guarantee wide band and high operating speed of the traveling-wave cathode-ray tubes. Scientific novelty and practical value. Models of insufficiently simulated slow-wave structures were created and their properties were analyzed. According to analysis and modeling results, variants of systems were selected that can guarantee the wide pass-band and high operating speed of the traveling-wave cathode-ray tubes. Using finite element method calculation... [to full text]

Aim and tasks of the work. The aim of this work is to investigate insufficiently analyzed variants of the electrodynamic super-wide-band slow-wave structures, create their models, improve methods of analysis, analyze properties of the systems and reveal potentiality of the traveling-wave cathode-ray tubes, slow-wave structures. In order to achieve the aim it is necessary: 1. To improve method for evaluation of non-linear distortions in the traveling-wave cathode-ray tubes and reveal possibilities of reduction of non-linear distortions. 2. To create models of the insufficiently analyzed variants of slow-wave structures and reveal properties of the slow-wave structures. 3. To reveal influence of periodical non-homogeneities on properties of slow-wave structures, simulate and reveal influence of transitions to properties of slow-wave structures and traveling-wav cathode-ray tubes. 4. To make investigation of potentiality of slow-wave structures and traveling-wave cathode-ray tubes and select variants of slow-wave structures that can guarantee wide band and high operating speed of the traveling-wave cathode-ray tubes. Scientific novelty and practical value. Models of insufficiently simulated slow-wave structures were created and their properties were analyzed. According to analysis and modeling results, variants of systems were selected that can guarantee the wide pass-band and high operating speed of the traveling-wave cathode-ray tubes. Using finite element method calculation... [to full text]

Titanium carbide (TiC) and titanium carbonitride (Ti(C,N)) are both common components in hard, wear resistant ceramic-metal composites, or cermets. In this study the intermetallic nickel aluminide (Ni3Al) has been used as a binder for the production of TiC and Ti(C,N) based cermets. These cermets offer several improved characteristics relative to conventional WC-based ‘hardmetals’, such as lower mass and improved oxidation resistance, which are also combined with high fracture resistance, hardness and wear resistance. The cermets were produced using an in-situ, reaction sintering procedure to form the stoichiometric Ni3Al binder, with the binder contents varied from 20 to 40 vol%. However, for high N content Ti(C,N) cermets, the wettability of molten Ni3Al is relatively poor, which leads to materials with residual porosity. Therefore various amounts of Mo2C (1.25, 2.5, 5 and 10 vol%) were incorporated into the Ti(C0.3,N0.7)-Ni3Al cermets, with the aim of improving the densification behaviour. Mo2C was found to improve upon the wettability during sintering, thus enhancing the densification, especially at the lower binder contents. The tribological behaviour of TiC and Ti(C,N) cermets have been evaluated under reciprocating sliding conditions. The wear tests were conducted using a ball-on-flat sliding geometry, with a WC-Co sphere as the counter-face material, for loads from 20 to 60 N. The wear response was characterised using a combination of scanning electron microscopy, energy dispersive X-ray spectroscopy, and focused ion beam microscopy. Initially, two-body abrasive wear was observed to occur, which transitions to three-body abrasion through the generation of debris from the cermet and counter-face materials. Ultimately, this wear debris is incorporated into a thin tribolayer within the wear track, which indicates a further transition to an adhesive wear mechanism. It was found that Mo2C additions had a positive effect on both the hardness and indentation fracture resistance of the samples, but had a detrimental effect on the sliding wear response of the cermets. This behaviour was attributed to increased microstructural inhomogeneity with Mo2C additions.

Modern image-guided surgery aids minimally-invasive, high-precision procedures that increase efficacy of treatment. This thesis investigates two research aims to improve precision and integration of intraoperative cone-beam CT (CBCT) imaging in guidance of head and neck (H&N) surgery. First, marker configurations were examined to identify arrangements that minimize target registration error (TRE). Best arrangements minimized the distance between the configuration centroid and surgical target while maximizing marker separation. Configurations of few markers could minimized TRE with more markers providing improved uniformity. Second, an algorithm for automatic registration of image and world reference frames was pursued to streamline integration of CBCT with real-time tracking and provide automatic updates per scan. Markers visible to the tracking and imaging systems are automatically co-localized and registered with equivalent accuracy and superior reproducibility compared to conventional registration. Such work helps the implementation of CBCT in H&N surgery to maximize surgical precision and exploit intraoperative image guidance.

Two experiments explore the application of plasmonic nanoparticles to cellular pathology. The first devised a platform by which gold-silver nanoparticles act as differentiable labels for cell surface receptors under dark field imaging. By conjugating particles of various constitutions with receptor-targeting antibodies, particles scatter characteristically according to their plasmon peak. The second experiment programmed receptor placement via the patterning of two substrates and used the binding of SERS nanoparticles to explore the quantification of such targets at high-density. On one substrate, anchor pairs established receptors at specified distances in order to define the relationship between scattering intensity and the distance between SERS particles. On the second, anchor regions are filled with increasing densities of receptors and the particle-saturated substrates are probed to relate scattering intensity to particle density. This should discover the density-threshold between linear and non-linear scattering and inform the quantification of particles in the exponential density regime.

Modern image-guided surgery aids minimally-invasive, high-precision procedures that increase efficacy of treatment. This thesis investigates two research aims to improve precision and integration of intraoperative cone-beam CT (CBCT) imaging in guidance of head and neck (H&N) surgery. First, marker configurations were examined to identify arrangements that minimize target registration error (TRE). Best arrangements minimized the distance between the configuration centroid and surgical target while maximizing marker separation. Configurations of few markers could minimized TRE with more markers providing improved uniformity. Second, an algorithm for automatic registration of image and world reference frames was pursued to streamline integration of CBCT with real-time tracking and provide automatic updates per scan. Markers visible to the tracking and imaging systems are automatically co-localized and registered with equivalent accuracy and superior reproducibility compared to conventional registration. Such work helps the implementation of CBCT in H&N surgery to maximize surgical precision and exploit intraoperative image guidance.

Two experiments explore the application of plasmonic nanoparticles to cellular pathology. The first devised a platform by which gold-silver nanoparticles act as differentiable labels for cell surface receptors under dark field imaging. By conjugating particles of various constitutions with receptor-targeting antibodies, particles scatter characteristically according to their plasmon peak. The second experiment programmed receptor placement via the patterning of two substrates and used the binding of SERS nanoparticles to explore the quantification of such targets at high-density. On one substrate, anchor pairs established receptors at specified distances in order to define the relationship between scattering intensity and the distance between SERS particles. On the second, anchor regions are filled with increasing densities of receptors and the particle-saturated substrates are probed to relate scattering intensity to particle density. This should discover the density-threshold between linear and non-linear scattering and inform the quantification of particles in the exponential density regime.