Based on the linear relationship between concentration of H2O2 and the decrease of electrochemiluminescence (ECL) intensity in a Ru(bpy)32+/TPA system, procedures for the indirect determination of glucose with a flow injection analysis were developed. By passing solutions of glucose through a FIA system containing a glucose oxidase (GOx) immobilized sol-gel column and an ECL system of Ru(bpy)32+ and TPA, glucose can be determined optimally with a detection limit of 1.0 £gM in a linear dynamic range of 1.0 ¡V 200.0 £gM. A repetitive injection of glucose (100 £gM) and human serum solutions gave satisfactory reproducibility with relative standard deviations of 1.3 (N=31) and 3.9 % (N=42) respectively. Interference due to the presence of ascorbic acid, uric acid or other reducible agents in solution can be corrected by passing sample solutions through another sol-gel column that contained no GOx. From the agreement between the contents of glucose in human serum and soft drink analyzed by the developed method and those obtained by the spectroscopy method based glucose assay kit and satisfactory recovery of glucose from interferent containing solutions, the feasibility of the developed method for real sample analysis was confirmed. One of the major purposes of this study was to develop new immobilization approaches and flow cell designs for the fabrication of regenerable ECL-based sensors with improved sensitivity, convenience and long-term stability. Silica particulates were used as immobilization support in ECL sensors for TPA and NAD(P)H and in biosensors for glucose and glucose-6-phosphate¡]G6P¡^. The first ECL flow cell was fabricated from a glass tube, and a platinum wire was used as working electrode held at +1.3 V. The volume of the flow cell was about 50 £gL. An Ag/AgCl electrode and a piece of Pt wire were used as the reference and counter electrode respectively and placed downstream of the working electrode. Ru(bpy)32+ immobilized silica particulates with 1/3 silica sol content showed the best performance for TPA determination, and the sensitivity of TPA determination was dependent upon the amount of Ru(bpy)32+ immobilized in silica particulates. The lowest level of analyte detected for TPA was 0.02£gM, and linear range was from 0.02£gM to 5£gM. Up to a certain concentration level, it was found that Ru(bpy)32+ was tightly held in silica particulates and did not leach out into aqueous solutions, even with continuous flow for up to ten hours. Ru(bpy)32+ immobilized silica particulates were characterized of well activity and high stability; that stored at 0¢J exhibited its original activity for up to one year. The second ECL flow cell was fabricated from a piece of epoxy block supported Pt electrode (1 ¡Ñ 2 cm) as counter electrode, a piece glass window and a polyethylene spacer with 78 £gL cell volume, two 2.0-cm length of 0.6-mm diameter platinum wires were used as working electrodes held at +1.1 V, and an Ag/AgCl electrode as reference electrode. All three electrodes were incorporated within the main body of the cell. One of the biosensor design packed Ru(bpy)32+ incorporated silica particulates in the ECL flow cell, and a glucose dehydrogenase (GDH) immobilized silica sol-gel column is placed between the sample injection valve and the flow cell. The ECL response to samples containing glucose and cofactor (NADP) results from the Ru(bpy)33+ ECL reaction with NADPH produced by glucose dehydrogenase. This ECL biosensor was shown applicable for both NAD+- and NADP+- dependent enzymes, where NADH detection ranged from 0.50£gM ¡V 5.0 mM NADH and NADPH detection ranged from 1.0£gM - 3.0 mM NADPH. Glucose can be determined in a linear dynamic range of 5.0 - 500 £gM. Another biosensor design immobilized glucose-6-phosphate dehydrogenase¡]G6PDH¡^onto the Ru(bpy)32+ -doped silica particulates through silica chemistry and then packed these particulates into the ECL flow cell. By passing samples containing G6P and cofactor (NAD) through the ECL flow cell, G6P can be determined in a linear dynamic range of 10.0 £gM-1.0 mM. The regenerable ECL biosensor was characterized of good reproducibility and well stability for flow injection analysis. A repetitive injection of NADH (100 £gM) and G6P¡]500£gM¡^gave satisfactory reproducibility with relative standard deviations of 2.8 %¡]N=105¡^and 2.8 % (N=40) respectively.

視覺辨識是極其快速而且正確的，逐步揭露作業可展示此一閃而過的動態視覺辨識歷程，本研究目的即在以此作業探討主角與地點在動態視覺辨識過程中相互影響的內涵。實驗一旨在建立主角與地點的視覺辨識基準線，結果發現主角比起地點只需累積較低空間頻率訊息即可完成正確辨識，得到物體優勢效果。實驗二旨在驗證物體與背景之間在視覺處理上的非獨立關係，透過操弄單獨呈現與同時呈現兩種視覺呈現方式，以主角辨識作業與地點辨識作業加以驗證。結果顯示對地點辨識作業而言，同時呈現情況比起單獨呈現情況只需累積較低空間頻率訊息即可完成正確辨識；對主角辨識作業而言，單獨呈現情況與同時呈現情況並無不同。除此之外，在單獨呈現情況下，仍獲得物體優勢效果。但在同時呈現情況下，物體優勢效果並不復見，反而是地點辨識優於主角辨識。實驗二結果支持物體與背景之間在視覺處理上為非獨立關係。實驗三進一步從「一致性效果」在促進層面以及抑制層面上的作用情況，探討物體與背景之間在視覺處理上相互影響的內涵。實驗三a結果顯示，在主角辨識作業中所得到的「一致性效果」，源於地點訊息對主角辨識在抑制層面的作用而來。實驗三b結果顯示，在地點辨識作業中所得到「一致性效果」，則源於主角訊息對地點辨識在促進層面與抑制層面的作用而來。實驗四進一步以同時呈報的作業方式，讓參與者對整張場景進行辨識，對於視覺系統所知覺到的主角內容與地點內容都需加以呈報，藉此再次驗證主角與地點處理的相互影響。其結果顯示在主角內容與地點內容呈報時，皆獲得「一致性效果」。除此之外，在一致情況與不一情況下皆獲得物體優勢效果。本研究以動態視覺處理模型中物體與背景平行處理且密切交換訊息之觀點解釋所得結果，並提出注意力分佈在此動態視覺處理歷程扮演重要角色。 / Visual recognition is a fast and accurate process. The present study adopted a progressive revelation task, which mimics the visual dynamics appropriately, to investigate the interaction of character and surroundings in the dynamic visual processing. Experiment 1 aimed to establish visual recognition curves for character and surroundings separately as baselines. The results showed that less amount of cumulated perceptual evidence was required for character than surroundings, so that it showed the object advantage effect. In Experiment 2, the non-independent relationship between the object- and background-related visual processes was verified. The performance of isolation condition with the character and surroundings presented in isolation was compared to the concurrent condition with the two presented concurrently. The results of the surroundings recognition task showed that less amount of cumulated perceptual evidence was required for concurrent condition than isolation condition. In contrast, for the character recognition task, there was no difference between these two conditions. These results supported the non-independent relationship between object- and background-related processes. Object advantage effect was replicated in the isolation condition but not in the concurrent condition, which meant that surroundings required less amount of perceptual evidence than character for visual recognition instead. In Experiment 3, interaction between object- and background-related processes was investigated by consistency effect from both the aspects of facilitation and inhibition effects. Results of Experiment 3a showed that consistency effect was only contributed by inhibition effect in the character recognition task. Results of Experiment 3b showed that both the facilitation and inhibition effects contributed to the consistency effect in the surroundings recognition task. In Experiment 4, participants were asked to report both the contents of character and surroundings. The results showed that consistency effects occurred in both of the content reports. And also the object advantage effect appeared in both of the consistent and inconsistent conditions. Overall, the results of the present study implied that object- and background-related visual processes operate in parallel while interchange information intimately at each level of the visual processing stages. The results also suggest that deployment of attention resource played an important role in the dynamic visual process.