A carência de instrumentos de pesquisa que avaliem a qualidade do ensino de ciências no Brasil é latente. A aplicação no Brasil de um instrumento internacional que permita avaliar os interesses e as posturas de jovens estudantes frente às ciências e tecnologias (C&T) é o principal objetivo deste trabalho e um passo importante na discussão desta questão. O ROSE - The Relevance of Science Education é um questionário aplicado a alunos da faixa dos 15 anos de diversos países que nos auxilia a confirmar hipóteses, entender padrões e a traçar tendências sobre o ensino de ciências. O desenvolvimento de uma versão brasileira do questionário ROSE, possibilitou este estudo no País. O instrumento foi aplicado em 2007 a 652 alunos das cidades de São Caetano do Sul/SP e Tangará da Serra/MT, trazendo elementos objetivos sobre a realidade do ensino de ciências em contextos brasileiros bem diversos. O estudo revelou que estes jovens acham a disciplina ciências interessante, mas por outro lado, têm um baixo interesse em exercer ciência profissionalmente. Suas análises evidenciam também a grande preferência das meninas por assuntos relacionados à saúde enquanto que a preferência masculina é por temas de tecnologia e física, ao lado daqueles vinculados à proteção ambiental. No outro extremo, existe um desinteresse, de meninos e meninas, nas questões de botânica e agricultura, bem como naquelas relacionadas à história da Ciência. Estes jovens alunos reconhecem a importância e se interessam pelas questões e desafios ambientais. Os alunos paulistas incluem-se como responsáveis pelas questões do meio ambiente e cobram maior envolvimento da sociedade na proteção ambiental, não acreditam que as C&T possam resolver todos estes problemas, mas são muito esperançosos em relação ao futuro do planeta e da humanidade. Os estudantes matogrossenses, diferentemente, excluem-se das responsabilidades pelos problemas ambientais e colocam nas mãos dos especialistas reger as mudanças necessárias, acreditam no poder das C&T para tais mudanças, mas declaramse menos confiantes no seu sucesso. O ROSE traz claras evidencias de que o ensino de ciências carece de mudanças e colabora com a intenção de redefinir as prioridades brasileiras para seu aprimoramento. Revela dados que, somados àqueles já registrados em pesquisas anteriores, permitem levar - com segurança - a discussão adiante. / The lack of research tools which really evaluate the quality of science teaching in Brazil is concealed. The application of an international instrument which grants the evaluation of young students\' interests and postures towards sciences and technologies (S&T) is the main purpose of this work as well as an important step related to the discussion of this issue. ROSE - The Relevance of Science Education - is a questionnaire applied to 15-yearold students from several countries which helps us confirm hypothesis, understand patterns and outline tendencies as to the teaching of sciences. The development of a Brazilian version of the ROSE questionnaire enabled this study in the country. The device was applied in 2007 to 652 students in the cities of São Caetano do Sul/São Paulo State and Tangará da Serra/Mato Grosso State, helping emerge objective elements about the reality of science teaching in quite different Brazilian contexts (urban/industrialized x rural/agricultural). The study revealed that these youngsters evaluate science study as interesting; however, the possibility of taking science as a professional career has shown low interest. Their analysis also made it clear that most girls show more interest in topics related to health while boys give preference for themes related to technology and physics, as well as those entailed to environmental protection. On the other hand, neither boys nor girls show much interest in botany or agriculture, much less in those related to Science History. These young students recognize the importance of and are interested in environmental matters and challenges. The São Paulo students accept their responsibility as to environmental issues and claim for a society\'s bigger involvement with environmental protection; they do not believe that the S&T can solve all these problems, either. However, they are full of hope towards the future of the planet and mankind. The students of the state of Mato Grosso, differently, do not include themselves as responsible for environmental problems and relegate to specialists the duty of taking the necessary changes. They also believe in the S&T power in terms of these changes; however, they confess to be less trustful in their success. The ROSE opens up clear evidences that science teaching needs changes and contributes to the intention of re-defining Brazilian priorities for the refinement of science teaching. It reveals data that, added to the ones already enrolled in previous researches, allow the follow-up of the discussion of this theme in safe grounds.

This diploma thesis deals with constructing of algorithms of consisting a puzzle and suggesting of the workplace for its imaging. There are studied two methods of forming a puzlle. First method is forming of the puzzle with a known pattern. For this method is used the Harris operator and the correlation. In the second method is the puzzle formed without a pattern. Fot this method is used the correlation. In the last part there is a mutual evaluation of these two methods.

This thesis is concerned with the Czech doctor's campaign "Thanks, we are leaving" on the background of the health care system in the Czech Republic. The campaign started in 2010 by the Czech doctors trying to focus on the working conditions, salary conditions, educational system and some failures of the health care system with the aim to improve it. The campaign resulted in February 2011 in a compromise between doctors and Ministry of Health. This thesis focuses especially on media and on the interest group LOK (Medical union trade club) which set the agenda. The perception of the campaign is ambiguous both for the public and for the doctors themselves. The individual milestones of campaign are explained by the theory of punctuated equilibrium from the authors Bryan D. Jones and Frank R. Baumgartner. Using many helpful methods such as content analysis of media messages, semi- structured interviews with particular actors, analysis of secondary sources, stakeholder analysis or analysis of selected events in health policy I explained the core events and actors who participated in this campaign. The theoretical concepts used in this thesis are: public policy in its multidisciplinary meaning, health policy and health care system, punctuated equilibrium theory, theories concerned with interest groups...

This thesis deals with content based automatic photo categorization. The aim of the work is to experiment with advanced techniques of image represenatation and to create a classifier which is able to process large image dataset with sufficient accuracy and computation speed. A traditional solution based on using visual codebooks is enhanced by computing color features, soft assignment of visual words to extracted feature vectors, usage of image segmentation in process of visual codebook creation and dividing picture into cells. These cells are processed separately. Linear SVM classifier with explicit data embeding is used for its efficiency. Finally, results of experiments with above mentioned techniques of the image categorization are discussed.

This diploma thesis is focused on the evaluation of six selected zoonoses with the occurrence in South Bohemia (campylobacteriosis, salmonellosis, listeriosis, tick-borne meningoencephalitis, Lyme borreliosis, tularemia) between the years 2003-2013. Campylobacteriosis and salmonellosis were evaluated the most common zoonoses in the South Bohemian Region during the monitored period. Their common feature is the alimentary transmission. Between 2003 to 2008 salmonellosis recorded the highest number of reported cases, on the contrary, campylobacteriosis has reported the highest number of occurrences since 2008. District of Czech Budweis showed the highest incidence of both zoonoses during the monitored period (4,139 cases of salmonellosis and 4,924 cases of campylobacteriosis). Another but not less important zoonotic is tick-borne meningoencephalitis, which incidence had the highest number in South Bohemian Region of all the regions of Czech Republic. The second part of the thesis is based on questionnaire studies focused on awareness of zoonoses and the issue of resistance and overuse of antibiotics. In total 479 questionnaires were evaluated. The survey findings may be equally described as interesting and positive, since the respondents had considerable awareness of the issue of antibiotic resistance.

L'activació sostinguda de Hog1 porta a una inhibició del creixement cel·lular. En aquest treball, hem observat que el fenotip de letalitat causat per l'activació sostinguda de Hog1 és parcialment inhibida per la mutació del complexe SCFCDC4. La inhibició de la mort causada per l'activació sostinguda de Hog1 depèn de la via d'extensió de la vida. Quan Hog1 s'activa de manera sostinguda, la mutació al complexe SCFCDC4 fa que augmenti l'expressió gènica depenent de Msn2 i Msn4 que condueix a una sobreexpressió del gen PNC1 i a una hiperactivació de la deacetilassa Sir2. La hiperactivació de Sir2 és capaç d'inhibir la mort causada per l'activació sostinguda de Hog1. També hem observat que la mort cel·lular causada per l'activació sostinguda de Hog1 és deguda a una inducció d'apoptosi. L'apoptosi induïda per Hog1 és inhibida per la mutació al complexe SCFCDC4. Per tant, la via d'extensió de la vida és capaç de prevenir l'apoptosi a través d'un mecanisme desconegut. / Sustained Hog1 activation leads to an inhibition of cell growth. In this work, we have observed that the lethal phenotype caused by sustained Hog1 activation is prevented by SCFCDC4 mutants. The prevention of Hog1-induced cell death by SCFCDC4 mutation depends on the lifespan extension pathway. Upon sustained Hog1 activation, SCFCDC4 mutation increases Msn2 and Msn4 dependent gene expression that leads to a PNC1 overexpression and a Sir2 deacetylase hyperactivation. Then, hyperactivation of Sir2 is able to prevent cell death caused by sustained Hog1 activation. We have also observed that cell death upon sustained Hog1 activation is due to an induction of apoptosis. The apoptosis induced by Hog1 is decreased by SCFCDC4 mutation. Therefore, lifespan extension pathway is able to prevent apoptosis by an unknown mechanism.