This comparative research looks at intermedia agenda-setting effects between Internet bulletin boards and traditional news media, such as daily newspapers and broadcasting, in both the United States and Korea. By examining this intermedia relationship and the flow of influence by Internet bulletin boards on traditional media during presidential campaigns in the two countries, this dissertation study attempts to extend our knowledge of intermedia agenda-setting research. In addition, it also investigates, in reverse, the effects of daily newspapers and broadcasting on Internet bulletin boards. Finally, attention is given to different types of discussion cultures in the two countries. Results of this dissertation research indicated that there are intermedia relationships between Internet bulletin boards and traditional news media, such as newspapers and broadcasting, at the first and second levels of agenda setting using cross-lagged correlation comparisons. More specifically, at the first level of agenda setting in the United States, the results explained only the influence of newspapers on Netizen opinions posted on Internet bulletin boards. In summary, the results concerning issue agenda in the United States indicate that the U.S. Netizen concentrates more on the issue agenda of newspapers than of broadcasting. In the second level of agenda setting in the United States, cross-lagged correlation comparisons not only indicated the influence of both newspapers and broadcasting on opinions posted on Internet bulletin boards, but they also clarified it in this research. Formerly, there was no attempt to examine attributes of the intermedia agenda-setting functions of the U.S. media. This research now provides an explanation of the apparent relationship between traditional media and the Internet. At the first level of agenda setting in Korea, the result of the cross-lagged correlation suggested that Korean newspaper and broadcasting issue agenda influenced Netizen opinions on Internet bulletin boards. As the result of second-level agenda setting in Korea showed earlier, cross-lagged correlation comparisons presented intermedia agenda-setting functions between both newspapers and broadcasting, and Internet bulletin boards with each other. These findings contrast with results in the United States. / text

Objectives: This investigation adapted the LIVE/DEAD� Baclight[TM] bacterial viability stain for the quantitative determination of bacterial cell viability of the aerotolerant anaerobes Porphyromonas gingivalis ATCC 33277 and Prevotella intermedia ATCC 25611. The Live/Dead stain was used to determine the influence of haem availability on the resistance of P. gingivalis and P. intermedia to the reactive oxygen species (ROS) superoxide anion and hydrogen peroxide and compare the sensitivities between the haem-requiring periodontal bacteria to ROS. Neutrophils use oxidative and non-oxidative killing mechanisms. During phagocytosis, neutrophils kill bacteria via a respiratory burst, producing ROS. P. gingivalis and P. intermedia are oxygen-tolerant gram-negative bacteria found in the gingival crevice. These bacteria express superoxide dismutase (SOD) activity, which extends some protection against superoxide radicals. Methods: Initially, experiments were performed to validate the reliability and accuracy of the fluorogenic Live/Dead stain using Escherichia coli ATCC 10798 (K-12), followed by experiments using P. gingivalis. The Live/Dead stain distinguishes viable:non-viable proportions of bacteria using mixtures of green (SYTO 9) and red (propidium iodide) fluorescent nucleic acid stains respectively. Bacterial cell viability was assessed with fluorescence microscopy and subsequently quantitative measurement using a fluorescence microplate reader (BMG Fluorostar plus Optima). P. gingivalis and P. intermedia colonies were subcultured from frozen cultures, in Tryptic soy broth (TSB) (Difco) and incubated anaerobically for approximately five days. They were further subcultured in pre-reduced TSB, supplemented with menadione 0.5[mu]g/ml (TSB-M) and either 5 [mu]g/ml haemin (Haem 5), 50 [mu]g/ml haemin (Haem 50) or without supplemental haemin (Haem 0). Cultures were grown anaerobically at 37�C to early stationary phase (approximately 48 hours). For experimental purposes, bacteria were harvested, washed and resuspended in 10 mM Tris-buffered saline (pH 7.5) containing peptone (TBS-P) (0.1 mg/ml), with a final adjustment to OD₅₄₀ [approximately equals] 2.0 (which corresponds to 1 x 10⁹ bacteria/ml). Bacterial suspensions were diluted ([approximately equals] 10⁸/ml) into TBS-P containing the fluorogenic viability stain (BacLight, Molecular Probes). Either pyrogallol (0.02 - 2 mM) or hydrogen peroxide (0.01 - 100 mM) was added (except to control tubes); tubes were vortexed for ten seconds and incubated at 37�C. Viability was monitored fluorimetrically for three hours. Results: For both P. gingivalis and P. intermedia, a pyrogallol concentration of 0.2 mM resulted in 80 to 90% cell death; and a hydrogen peroxide concentration of 10 mM killed approximately 80 to 90% of cells. Irrespective of the haem status, no significant difference was determined between the overall maximum rate of killing of P. gingivalis and P. intermedia, in their response to either superoxide or hydrogen peroxide; with the exception that the P. intermedia Haem 0 group was significantly less susceptible to hydrogen peroxide than the P. gingivalis Haem 0 group. For the majority of the experiments, there was no significant difference between final bacterial cell viability in the Haem 0 and Haem 5 cells for both species, after 3 hours exposure to various concentrations of ROS. However, the Haem 50 cells showed a significant increased susceptibility (albeit, a small difference) to both hydrogen peroxide and superoxide. Conclusions: The Live/Dead bacterial viability stain provided a valuable method to monitor "real-time" killing, avoiding the difficulties associated with culture-based methods for assessing viability. Haem availability had no clear influence on the resistance to ROS of either P. gingivalis or P. intermedia Haem 0 and Haem 5 cells. The Haem 50 cells showed a very slight increase in susceptibility to hydrogen peroxide and superoxide. Although P. intermedia may be isolated in significant numbers from healthy gingivae, as well as from periodontally diseased sites, it was no more resistant to ROS than was P. gingivalis, which is associated with periodontal lesions and difficult to cultivate from relatively healthy (more oxygenated) sites. This suggests that resistance to ROS does not contribute to the ecological distinction between these two species. The finding that haem availability did not influence sensitivity implies that these bacteria do not accumulate haem for the purpose of protection from ROS.

Thesis (MScFoodSc)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: In light of the limited and inconsistent supply of good quality honeybush tea, a species-specific sensory profile and the physicochemical characteristics of Cyclopia intermedia (honeybush) tea were determined to ultimately establish the optimum fermentation parameters for this herbal tea on laboratory-scale and to validate these findings on commercial-scale. The characteristic sensory profile of C. intermedia can be described as sweet tasting and slightly astringent with a combination of “fynbos-floral”, “fynbos-sweet”, “fruity” (specifically “apricot jam”, “cooked apple”, “raisin” and “lemon/lemon grass”), “woody”, “caramel/ vanilla” and “honey-like” aromas. The flavour can be described as distinctly “fynbos-floral”, “fynbos-sweet” and “woody”, including hints of “lemon/lemon grass” and “hay/dried grass”. The results of the sensory study were used to create a C. intermedia sensory wheel and lexicon, and an elementary grading system that categorised samples into “good”, “average” and “poor” sensory quality was proposed. Physicochemical parameters, i.e. soluble solids (SS) content, absorbance as a measure of colour, and turbidity, were evaluated as possible rapid predictors of sensory quality. High SS content, absorbance and turbidity correlated strongly with “poor” sensory quality. A linear relationship existed between the physicochemical parameters. The effect of fermentation temperature (70, 80 and 90°C) and time (12, 16, 24, 36, 48 and 60 h) on the sensory and physicochemical characteristics of C. intermedia was determined on laboratory-scale. Increasing fermentation time increased the intensity of positive sensory attributes, while decreasing the intensity of negative sensory attributes. The SS content, colour and turbidity of infusions decreased with increasing fermentation time, while the SS content and turbidity of infusions increased with increasing fermentation temperature. Fermentation at 90°C for 36 h on laboratory-scale produced C. intermedia with the best sensory properties, while preserving the SS content and colour of infusions. Fermentation at 70°C and 80°C required longer fermentation times for development of positive sensory attributes. Fermentation at 90°C was subsequently validated on commercial-scale. Laboratory-scale fermentation of the same batches of plant material was also carried out concurrently to allow direct comparison of the scale of fermentation on tea quality. Commercial-scale fermentation, despite increased variability as a result of increased batch volumes and heating difficulties, produced C. intermedia of “good” sensory quality after 24 and 36 h of fermentation. Increasing fermentation time had little effect on the SS content and colour of infusions of tea produced on commercial-scale, but turbidity increased significantly after 36 h. Thus, to produce C. intermedia with consistently good quality on commercial-scale, fermentation at 90°C for 24 to 36 h is recommended. Increasing fermentation time past 48 h should be avoided to prevent turbidity and the development of sensory attributes characteristic of over-fermented tea. However, due to the large variability of commercial-scale honeybush tea production, it is recommended that each batch be monitored between 24 and 36 h to determine when optimum fermentation has been obtained. / AFRIKAANSE OPSOMMING: Beperkte en wisselvallige beskikbaarheid van goeie gehalte heuningbostee noodsaak die optimisering van fermentasie parameters vir Cyclopia intermedia. Optimisering van fermentation parameters is op laboratorium skaal gedoen, gevolg deur validasie van die parameters op kommersiële skaal. Vooraf is die spesie-spesifieke sensoriese profiel en die fisies-chemiese eienskappe van C. intermedia tee bepaal. Die kenmerkende sensoriese profiel van C. intermedia kan beskryf word as soet en effens vrank met 'n kombinasie van "fynbos-blomagtige", "fynbos-soet", "vrugtige" (spesifiek "appelkooskonfyt", "gekookte appel", "rosyntjie” en “suurlemoen/sitroen gras"), "houtagtige", "karamel/vanilla" en "heuningagtige" aromas. Die smaak kan beskryf word as "fynbos-blomagtig", "fynbos-soet" en "houtagtig", met 'n tikkie "suurlemoen/sitroen gras" en "hooi/gedroogde gras". Die resultate van die sensoriese studie is gebruik om 'n C. intermedia sensoriese wiel en leksikon, asook 'n basiese graderingstelsel wat tee monsters in "goeie", "gemiddelde" en "swak" sensoriese kwaliteit klassifiseer, te ontwikkel. Fisies-chemiese parameters: oplosbare vastestof (SS) inhoud; absorbansie as 'n maatstaf van kleur; en troebelheid, is geëvalueer as moontlike indikasies van sensoriese kwaliteit. Hoë SS inhoud, absorbansie en troebelheid waardes het sterk met "swak" sensoriese kwaliteit gekorreleer. 'n Lineêre verwantskap bestaan tussen die fisies-chemiese parameters en kwaliteit. Die effek van fermentasie temperatuur (70, 80 en 90°C) en -tyd (12, 16, 24, 36, 48 en 60 h) op die sensoriese en fisies-chemiese eienskappe van C. intermedia is op laboratorium skaal bepaal. Verlenging van fermentasie tyd het die intensiteit van die positiewe sensoriese eienskappe verhoog, terwyl dit die intensiteit van negatiewe sensoriese eienskappe verminder het. Die SS inhoud, kleur en troebelheid van die tee het met verlengde fermentasie tyd afgeneem, terwyl die SS inhoud en troebelheid met verhoging van fermentasie temperatuur toegeneem het. Fermentasie by 90°C vir 36 h op laboratorium skaal het tee met die beste sensoriese eienskappe geproduseer, met behoud van die SS inhoud en kleur. Fermentasie by 70°C en 80°C het 'n langer fermentasie tyd vir die ontwikkeling van positiewe sensoriese eienskappe vereis. Fermentasie by 90°C is daaropvolgens op kommersiële skaal uitgevoer, met gelyktydige laboratorium skaal fermentasie van dieselfde plantmateriaal lotte om die direkte effek van die skaal van fermentasie op tee kwaliteit te bepaal. Kommersiële fermentasie, ten spyte van verhoogde wisselvalligheid as gevolg van groot volumes tee en probleme met verhitting, het tee van "goeie" sensoriese kwaliteit na fermentasie periodes van 24 en 36 h geproduseer. Verlenging van fermentasie tyd het min uitwerking op die SS inhoud en kleur van kommersiel gefermenteerde tea gehad, maar troebelheid het beduidend na 36 h toegeneem. Fermentasie by 90°C vir 24 - 36 h word gevolglik aanbeveel om tee met goeie gehalte op kommersiële skaal te produseer. Fermentasie vir langer as 48 h moet vermy word om troebelheid te voorkom en die ontwikkeling van sensoriese eienskappe kenmerkend van oor-gefermenteerde tee te vermy. As gevolg van faktore wat groot variasie in kommersiële skaal heuningbostee produksie kan teweegbring, word aanbeveel dat elke produksielot tussen 24 en 36 h gemonitor word om die optimum fermentasie tyd te bepaal.

The present article is about the constitutional interpretation theories. In the introduction, the author gives us a general vision of the present debate aroused around them. “Is the interpretation that conditions Constitution or is the Constitution that conditions interpretation?” comes to be the central question proposed by the article. To reach a possible answer, the author guides us through the world of constitutional interpretation theories, analyzing each one exhaustively and concluding with a new proposition about a general theory of constitutional interpretation, using the most important elements of each one of the theories previously exposed. / El presente artículo versa sobre las distintas teorías sobre la interpretación del texto constitucional. En la introducción, el autor nos da una visión general de la actualidad del debate suscitado alrededor de estas. “¿Es la interpretación la que condiciona a la Constitución o es la Constitución la que condiciona a la interpretación?” viene a ser la pregunta central propuesta en el artículo. Para llegar a una posible respuesta, el autor nos guía a través del mundo de las teorías interpretativas constitucionales, analizando a cada una de manera exhaustiva y finalizando con una propuesta propia de teoría general de interpretación de la Constitución, haciendo suyos los elementos más importantes de cada una de las teorías previamente expuestas.

Mycelial pellet formation by filamentous fungi is one of the most researched topics in fungal biotechnology research. Pellets are generally formed as a result of a complex interaction process through the influence of many cultivation factors such as inoculum size, pH, dissolved oxygen level, agitation system, nucleating agents, additives, trace metals, CO2, temperature, reactor types, carbon substrate, rheology, culture modes, fermenter geometry, nitrogen and phosphate levels etc. Each factor has varying effects on the growth morphologies of different fungal species. Fungal growths in the form of pellets have several advantages and pose a potential solution to overcome the problems associated with the filamentous fungal growth in large scale industrial bioreactors. The aim of the present work was to study pellet formation of edible filamentous fungus Neurospora intermedia, focusing on the molecular aspects of the fungal pellets with special interest to investigate the role of cell signaling second messenger cyclic 3', 5’-adenosine mono- phosphate (cAMP). It was found that Neurospora intermedia stimulate cAMP in the pellet form than filamentous form. The industrial applications of fungal pellets for generating value added products were also studied and observed fermentation in individual and co fermented first and second-generation ethanol substrate, showed an ethanol yield maximum of 0.25 ± 0.05 g/g dry substrate. The growth of fungal pellets in presence of inhibitors (such as acetic acid, HMF and furfural) resulted in about 11% to 45% increase in ethanol production as compared to filamentous forms, at similar growth conditions in the liquid straw hydrolysate.

My thesis tested efect of mycorrhizal fungy on herbal cutting. There was two terms propagation of four species. Syringa meyeri 'Palibin', Forsythia x intermedia 'Maluch', Cornus alba and Berberis thunbergii 'Rose Glow'. Experiment had two variantion and three repeats. Plants couldn't be nursed. Most of the results are inconclusive, because of the high losses. For species of Syringa meyeri 'Palibin' was demonstrated high influence of mycorrhizal fungi. There were also differences in terms. Overlay film had a positive impact only on Cornus alba. For species Cornus alba and Forsythia x intermedia 'Maluch' second term was more successful. When propagation of mycorrhiza has high potential.

The aim of my thesis was experimental prove of the influence of mycorrhizal product in the production of nursery propagation by herbaceous cuttings. On trial were selected three plant species, Deutzia gracilis 'Nikko', Forsythia x intermedia 'Maluch' and Syringa meyeri 'Palibin'. The experiment was based on two dates, and was conducted of the Institution of cultivation and reproducing of garden plants of Garden faculty of the Mendel University in Lednice. Evaluation was carried out in two variants, after three repetitions. The first variant was a control without mycorrhizal product, the second was supplemented with mycorrhizal fungi. It evaluated the number of roots, number of branched roots, cuttings and mortality rate of colonization. Differences in rooting and branching roots were among species, and between terms. The positive effect of mycorrhizal fungi was at Deutzia gracilis 'Nikko' and Forsythia x intermedia 'Maluch'.

Ten years ago, the only contenders for the role of setting the fashion media agenda were mainstream fashion media magazines such as Vogue. With the evolution of blogging and the popularity of blog sites associated with magazine websites, a new contender was born: the fashion-themed blog. Now mainstream fashion media outlets and fashion bloggers sit side-by-side in the front row at designer fashion shows covering fashion news and trends. This study focused on ten elite fashion-themed blogs (fashion blogs) reporting on women’s fashion and ten mainstream fashion media websites during the summer 2016 fashion season looking retrospectively at the fashion forecast from October 2015 New York Fashion Week. The study examined how media outlets respond to fashion blogs and whether the blogs were used as sources by mainstream fashion media or vice a versa. This study has filled a research gap by conducting the first analysis examining fashion blogs through the lens of agenda setting. This research uncovered a media landscape in transition with fashion bloggers and mainstream fashion media communicating in a new and different way. Communicating fashion news is no longer a one-way street controlled by mainstream fashion media magazines. Instead it is a two-way street where each outlet uses the other and neither plays the role of lead agenda setter. This new synergy suggests the acceptance of fashion blogs as credible sources for fashion news and potential agenda setters for the fashion community. The relationship between bloggers and mainstream fashion media also creates excitement regarding trends because fashion bloggers have the ability to instantly comment and generate buzz on a trend when the mainstream fashion media are reporting on other issues or waiting for their magazines to be published. Out of the five trends studied, denim was the leading trend with 44 percent of the posts or articles discussing denim. With every trend, bloggers were present in the five earliest published posts or articles and continued to post on the trends throughout the study period. Style.com dominated the mainstream fashion media landscape, with 25 published articles. For fashion bloggers, Gal Meets Glam and Atlantic Pacific tied with 19 blog posts each. Over 57.6 percent of the total blog posts and blog articles linked to fashion designer websites where readers could purchase the trend being discussed. Fashion bloggers did not quote sources in their posts; 74 blog posts and articles recognized no sources at all. Understanding the new relationship between fashion bloggers and fashion magazine websites will help guide future reporters of fashion and fashion trends.

The goal of this thesis is to describe the basic characteristics of intermedia agenda setting in the news broadcasted by Czech Radio using quantitative content analysis. The theoretical part of the thesis, which precedes the analysis, puts the concept of intermedia agenda setting into the wider context of agenda setting research, introduces some of the most important studies presented in this area, and provides explanations of related concepts such as gatekeeping or news values. In the empirical part of the thesis are first analyzed data concerning the frequencies of references to individual czech media outlets, then results of correlation analyzes describing the relationship between these references and the thematic agenda of Czech Radio are presented.

Scholars of intermedia agenda-setting have examined how news organizations can affect one another's content, but research is lacking on the influence of foreign news programs on fledging media outlets, such as Rwanda Television (RTV). A quantitative content analysis conducted between October and December 2016 indicates that media outlets in core countries dominated RTV's international news edition, which depended mostly on foreign programs, mainly from Euronews and Al Jazeera English. The 2016 U.S. election was the predominant topic. More than half the stories had a negative tone. In addition, qualitative interviews with the RTV editorial team revealed that the main factors influencing story selection were proximity, prominence, impact, cultural values, and relevance. Cross-lagged correlation coefficients indicated that both RTV and two Rwandan newspapers—igihe.com and Imvaho Nshya—focused on the same news topics but with slightly different sources. In Rwanda, the defining factors for this intermedia agenda-setting are not media type, ownership, or technology, as other studies have found, but institutional barriers, language, and the globalization of news. Faced with limited finances and a shortage of trained journalists, fledging media organizations in Rwanda will continue to depend on big media for their daily international news. Strategies are suggested for helping to break the cycle of foreign media domination and news homogenization in Rwanda