Presented diploma thesis is pursued to the topic of conceptual evolution of civil protection in the last century's 50's to 70's period. This period of time, population safety was secured by so called Civil Defence. Noticeable atribute of all the Civil Defence measures impacting population was mainly protection against mass destruction weapons. That is why protection against mass destruction weapons is given the most attention in this diploma thesis. Target of the thesis is historical analyse concerning Civil Defence and its evolution in Czechoslovakia in noted period. Out of it the thesis sketches also general formation and evolution of civil protection in previous period. Diploma thesis is done based on study of available literature and mainly based on accessible sources from military archive in Prague. Thesis is divided to six chapters, chronologically describing the most important milestones in Civil Defence conceptual evolution. Emphasis is laid on description of its organisational structure and way of population protection against mass destruction weapons. During studies of these sources, the emphasis was laid also on military evaluation of country territory according to level of expected threat by aerial attack that time, or later by nuclear weapon of mass destruction in case of war conflict....

Nuclear DNA content (genome size) is one of the basic characteristics of living organisms. In the Angiosperms, the range of genome size is 2,300-fold, which raises questions about the causes and consequences of this tremendous variation. This thesis deals with genome size in plants from the level of intraspecific homoploid variation, through intraspecific ploidy variation, to interspecies comparisons. On various study systems we investigated the dynamics and ranges of genome size variation, tried to reveal possible associations between genome size and selected biological traits, and assessed the extent to which differences in genome size are manifested at the ecological and evolutionary level. As a means of estimating genome size we applied flow cytometry (FCM). In Taraxacum stenocephalum we conducted a detailed study of its enormous genome size variation. We carried out crossings of parents with various genome sizes and compared these parental genome sizes with those of F1 offspring. We also attempted to reveal the association of genome size with various growth traits. In Galium valdepilosum and Arabidopsis arenosa we carried out an extensive flow-cytometric ploidy level screening and compared the distribution and ecological preferences of detected cytotypes. We studied the Andean genus...

The present diploma thesis aims to compare the approach of the Bush and Obama administrations towards the role of nuclear weapons in the United States security strategy. The author focuses at the Nuclear Posture Review (NPR) reports from 2001 and 2010, employing a detailed comparative analysis of their respective content as well as their implications. The analysis concentrates at broader conceptual issues as well as very concrete steps related to specific elements of the United States strategic arsenal. The author of the thesis argues that the current political discourse which attributes a nearly revolutionary character to the approach of the Obama administration to the United States nuclear policy does not fully match the actual dimension of change between the NPR of 2001 and 2010. In fact, the evolution of the United States nuclear strategy maintains its own dynamics in many aspects. Additionally, the present diploma thesis also tries to analyze both documents within the context of the evolution of the United States nuclear strategy since the 1990s which allows the author to identify the shifts as well as the patterns of continuity in the US nuclear policy in the post-Cold War period.

Master thesis deals with the concept of "rogue states" and strategies of the United States that are used towards rogue states. Concretely, attention is focused on two such labeled countries - Iran and the Democratic People's Republic of Korea. Strategies used by the United States are further observed during the presidencies of George W. Bush and Barack Obama and with regard to two typical characteristics of rogue states - effort to acquire weapons of mass destruction and the support of terrorism. Despite expectations, it appears that strategies used by the United States differ significantly and they are often influenced by historical experiences and strategic interests. Bush's and Obama's foreign policies were significantly different on the rhetorical level. However, as shown in the thesis there can be found certain continuities between the strategies towards rogue states.

The thesis "Nuclear Iran Oversecuritized? The Politics of Benjamin Netanyahu's Speeches" is concerned with the Israeli Prime Minister Benjamin Netanyahu's speeches held in front of the UN, American Congress, Jewish lobby in the US AIPAC and foreign audience by several special occasions. It is based on the assumption that language is not only a neutral communication tool, but also a political tool serving the aim of achieving political goals. The aim of the thesis is to identify the linguistic tools of categorization, legitimization and securitization which Netanyahu used in order to persuade the international audience about the imminence and existential character of the threat posed by Iranian nuclear programme, and finally to achieve its elimination or at least tough punishment for Iranian non-compliance. Moreover, the thesis provides the reader with the overall context, namely the effect of Netanyahu's speeches, the nature of Iranian nuclear programme and its consequences for the situation in the Middle East.

5 Abstract Genetic mechanisms of regulation of gene expression form the basis for proper development, function of organisms and their responses to variable life conditions. However, they are relatively slow. Life processes that require a fast response to the changing environmental and metabolic conditions are mostly executed on the level of proteins especially their posttranslational modifications and protein- protein interactions.The goal of the experimental work that led to the presented thesis consisted in exploitation of the model organism Caenorhabditis elegans for analysis of regulation of gene expression by transcription factors from the protein family of nuclear receptors. The model system C. elegans enables very efficient experimental procedures in the field of genetics, genomics and functional analysis of phenotypes. In the experimental work connected with this thesis, I studied the regulation of gene expression under specific experimental conditions from the perspective of advanced functional proteomics and I focused on the employment of separation methods and methods of advanced proteomics, especially by mass spectrometry.In the first part of the work, I characterized the nuclear receptor NHR-60 on the protein level. This nuclear receptor is expressed as two protein forms with a mass of 50 kDa...

The aim of the dissertation is proposing and solving energy convection systems for nuc-lear power plants with a sodium fast reactor of the 4th generation. The first part of the dissertation deals with collection and evaluation of information available about nuclear power plants with sodium fast reactor which use nuclear or non-nuclear reheating to increase thermal efficiency. On the basis of the acquired information, thermal schemes are developed and thermal effi-ciency is determined for the systems working in both Rankine thermal cycle and Brayton thermal cycle. In the further part of the dissertation thermal calculation of the reheater for nuclear and non-nuclear reheating is made for the systems working in Rankine thermal cycle. At the end of this dissertation, an apparatus suitable for these systems is suggested and the systems are evaluated in terms of technical implementation and nuclear safety.

The theme of the dissertation is a change in an approach to securing fire protection in the Temelín Nuclear Power Plant after the accident in the Fukushima Nuclear Power Plant. The theoretical part is an introduction to fire protection. It especially points out the statutory obligations applicable for both the public and the private sector, which are transferred to the companys fire rescue corps too. At the same time, fire protection is interconnected with legislation and specified in greater detail in EU countries. The theoretical part describes the process of building a uniform information system, safety-related issues and prevention of dangerous situations. The theoretical part summarizes the history of the nuclear energy industry, from the discovery of the disintegration of the uranium nucleus in 1938 and the first controlled reaction of uranium liberation by splitting a nucleus in 1942, when, at the same time, the first reactor was built. Unfortunately, this discovery did not only familiarize mankind with positive aspects associated with a controlled reaction, but it also showed the dark side of nuclear energy in the form of atomic bombs used mainly in the course of the World War II. After the World War II, civil defence organizations were gradually founded in the world. The main goal of all these organizations was to prevent nuclear weapons, nuclear power plants and nuclear fuel from being spread. At the present time there are three strong international organizations dealing with nuclear fission. Despite all the efforts to build a system of radiation protection, environmental protection and impacts on living organisms were not elaborated fully. First, passive attitudes aimed at protective equipment were the central theme, and these passive attitudes have been reflecting in an active approach to environmental protection in the past few years, the goal being to eliminate phenomena giving rise to threats to the environment and life as such. The actual history of nuclear power plants is very complex and complicated. The principle of all these power plants, no matter what type of reactor their operation is based on, is always nuclear fission the trouble is that in this case any wrong handling or storage has immense consequences for the whole world's population. The development of the nuclear energy industry in the Czech Republic was slow, which was caused by the occupation of the Jáchymov uranium mines by the Soviet Army after the World War II. Based on an agreement with Czechoslovakia, the Soviet Army exported the uranium extracted to the Soviet Union and left just 10% of it in the territory of Czechoslovakia. At that time, nuclear power plants began to be built in Czechoslovakia, such as the Jaslovské Bohunice Nuclear Power Plant, Dukovany Nuclear Power Plant and the Temelín Nuclear Power Plant as the last one, whose construction was started in times of Czechoslovakia, but which was completed and put into operation in times of the existence of the Czech Republic. The research is focused on emergency events which were the cause of the accident in the Fukushima Nuclear Power Plant. Based on an FMEA (failure mode and effects analysis), the issues related to the activities of the company's fire rescue corps were specified. Another analysis is focused on the evaluation of outside and inside factors and evaluation of strengths, weaknesses, opportunities and threats to the company's fire rescue corps. Utilizing an FMEA, possible risks and failures are detected. The goal of this analysis is to eliminate risks and increase safety. The result is objective evaluation and increase in safety within the Temelín Nuclear Power Plant. A considerable negative aspect of this analysis is that it is very time consuming and varies in time due to the changeability of the factors monitored. The result of all the work is the evaluation of the existing state of the company's fire rescue corps through a SWOT analysiS.

This diploma thesis on the application of logical, empirical and statistical methods defined the role of the complementarity principle in the preparation of experts in the field of the protection of population in the framework of a quantitative research. The complementarity principle is characterized in the thesis in general (working with two expressions, both of them are necessary to explain the nature of the problem but cannot be used simultaneously, e.g. expressions "analysis" and "synthesis"). The diploma thesis then defined the physical aspect of the complementarity principle the wave-corpuscular dualism, the position and the momentum, the energy and the time. The comparison of the basics of the protection of population with the structure of the physical aspect of the complementarity principle followed. The performed comparison was adjusted to the needs and possibilities of future experts in the field of the protection of population on the basis of the theory of the curriculum process. The steps are clear from the set of objectives which were fulfilled gradually in the thesis: 1) To systematically describe the general form of the complementarity principle and its physical application from the point of view of the theory of the curriculum process. 2) To create a structure of the system of emergencies from the point of view of a scientific field of the protection of population and to focus on its educational aspect for the preparation of experts. 3) To perform the comparison of the physical aspect and emergencies with the general form of the complementarity principle. To transfer the findings, a theory of curriculum process was used. The link between a more generally approached complementarity principle and the protection of population was explained in the framework of a conceptual curriculum. The theory connecting these two different fields is the more generally approached complementarity principle and especially Bohr's complementarity principle linking not only the wave-corpuscular dualism but also Heisenberg's uncertainty principle. The physical aspect of emergencies was adjusted to the possibilities and needs of students (the intended curriculum) and written down in an educational text which was provided to students (the project curriculum). The practicality of the educational test was verified by an educational test the results of which were statistically processed (implemented curriculum). The statistical analysis of the test results confirmed the second hypothesis of this work. "The knowledge of future experts in the field of the protection of population will have, from the point of view of the role of the complementarity principle in their preparation, a theoretical division which will be close to the normal division." By confirming this hypothesis, the correctness and applicability of the curriculum process were confirmed as it was assumed by the first hypothesis: "Using the stages of a curriculum process, an educational basis of the role of the complementarity principle can be created within a quantitative research." The contributions of this work can be summarized by following points: 1) The use of an educational text in the preparation of experts in the field of the protection of population (a practical contribution of this diploma thesis). 2) The improvement of the applicability of the theory of the curriculum process in the field of the protection of population which has not been researched so far (the improvement of the theory of the curriculum process). 3) The definition of the link of the physical aspect of the complementarity principle to the general form of this principle (the theoretical contribution of this diploma thesis).

Pavel Jůda - Abstract The cell nucleus is a complex cellular organelle. The nucleus and nuclear processes are organized into functionally and morphologically separated nuclear subcompartments. This thesis is particularly concerned with the three following nuclear subcompartments: sites of DNA replication, Polycomb bodies and nuclear inclusions constituted of inosine monophosphate dehydrogenase 2 (IMPDH2). First, we examined the relationship between MCM proteins and DNA replication. Using immunofluorescent labeling of cells extracted prior fixation and applying cross-correlation function analysis, we showed that MCM proteins are present at the sites of active DNA synthesis. Our results contributed to the solving of the first part of so-called MCM paradox. Second, we studied the structural basis of the Polycomb bodies. Based on fluorescence microscopy studies, Polycomb bodies have been considered to be the nuclear subcompartments formed by the accumulation of Polycomb proteins in the interchromatin compartment. In our work, using correlative light electron microscopy and experimental changes in macromolecular crowding, we clearly showed that a Polycomb body is a chromosomal domain formed by an accumulation of heterochromatin structures, rather than a typical nucleoplasmic body. Third, we were interested in...