This master thesis focuses on the phylogeny of hybridogenous and polyploid genus Curcuma from family Zingiberaceae using Next-Generation Sequencing data from hundreds to thousands nuclear loci. This approach seems to be better than widely used cpDNA and ITS sequencing especially in the case of hybridogenous and polyploid groups. Data for phylogeny reconstruction were generated using Hybridization-based sequencing (Hyb-Seq) method which combines target enrichment and genome skimming strategies. Data analysis was performed primarily using pipeline HybPhyloMaker especially created for Hyb-Seq data analysis. Twenty-seven species from the genus Curcuma and three outgroup species were sequenced in this work. Phylogenetic trees based on all 1 154 and 811 selected nuclear low- copy genes show high support values of all nodes which is in contrast to plastome and rDNA phylogeny with lower support values in some nodes and incongruences in topology compared to low-copy genes phylogeny. Phylogenetic networks inferred from low-copy genes, lineage movement analysis and monophyly tests agree with published hypotheses of interlineage hybrid origin of three species - C. vamana, C. myanmarensis and C. roscoeana. These analyzes show likely hybrid origin of C. candida too with parents from the group Curcuma I and basal...

The objective of the thesis is to characterize the nuclear energy in the european area with the focus on nuclear energy in France. The sector of nuclear energy is now experiencing a new period, so-called "nuclear renaissance". Many european countries decided to rebuild the present nuclear power sites or to construct new ones. Nuclear energy is the most advantageous solutions for many european countries because it decreases external energy dependence and it helps to diminish the carbon dioxide emissions. In the connection with the Czech Presidency in the European Union, it was made proposal for the "Nuclear Security Community" which has to unify the security level of member states of the EU and to intensify the mutual cooperation in the domain of nuclear energy. France represents world nuclear leader thanks to efficiently operating oragnizations, excellent results in nuclear research, realization of nuclear fuel recycling and strong government support. Nucler energy in France can serve as an example for four recommendations to other european countries: government support, efficiently working research institutions, global cooperation between all subjects participating on the formation of nuclear energy and active involvement in the international cooperation in nuclear energy. In spite of constantly high initial investment in construction of nuclear power sites, the nuclear energy seems to experience a positive future.

This thesis is focused to the economic analysis of the nuclear fuel strategy change at nuclear power plant Temelin, where the change to 18 months fuel cycle is considered. The introduction of theses is aimed to the identification of direct economic aspects for the financial analysis. Nuclear fuel strategy change affects operation of power plant as a whole and affects production of spent nuclear fuel. Therefore the economic analysis is needed for include all social costs of the change. The conclusion of thesis is aimed to the risk analysis of the nuclear fuel strategy change. Risk analysis is performed by Monte Carlo simulation.

The search for Accident tolerant fuels (ATF) which is the first part of this thesis is currently one of the most actual topics in the field of nuclear fuels. These fuels must be first successfully tested in operational and also accident conditions for their possible inclusion in commercial use. Following part of the thesis specifically focuses on the boiling crisis in nuclear reactors which can damage the nuclear fuel cladding. Therefore, it is necessary to know the critical heat flux value and the departure from nuclear boiling ratio. Calculations which determine critical heal flux value are placed in the practical part of the thesis. Calculations are compared with the data obtained during experiments. The ALTHAMC12 and the other correlations which are based on the previous measurements are used for the computational analysis.

Author´s name: Bc. Stanislav Klimek School: Brno University of Technology, Faculty of Mechanical Engineering, Energy institute Title: Determining the life storage of a carbon steel cask Consultant: Prof. Ing. Oldřich Matal, CSc. Number of pages: 70 Year: 2009 The assignment of this diploma thesis is to estimate the lifetime of spent fuel container made from carbon steel grade. This container is designed for deep geological disposal of spent nuclear fuel. Basic mechanism of corrosion are described in detail in the first part. Further on, this work deals with the other specific phenomena and influences, which affect at corrosion of steel in conditions of a deep geological repository. Heat, radiation and surroundings are considered of particular importance. In the following part an estimate of the lifetime of model container is introduced, which is affected by temperature and radiation. Here recommendations for protection of container are introduced, arising from the model calculation. Finally, the relevancy of incidence of particular parameters is evaluated, which affect the corrosion.

This master‘s thesis deals with radiation damage of reactor pressure vessels, specifically of NPP Dukovany Unit No. 3. In general damage mechanisms of reactor steels are described and possibilities of monitoring of material degradation and its recovery used at NPP’s all over the world are mentioned as well. A practical part of the thesis is focused on interpretation of analyses carried out with the assistance of MOBY DICK code. The ground of these analyses is a neutron fluence value development on different locations of RPV for the whole life of operation up to 24th cycle. The analyses results are put into context with performed in-service inspections. The thesis follows up with neutron fluence computation for the future cycles containing new types of nuclear fuel up to 34th cycle. The outcome of practical part of the master‘s thesis is a comparison between new types of nuclear fuel with respect to radiation damage of RPV’s.

Pavel Jůda - Abstract The cell nucleus is a complex cellular organelle. The nucleus and nuclear processes are organized into functionally and morphologically separated nuclear subcompartments. This thesis is particularly concerned with the three following nuclear subcompartments: sites of DNA replication, Polycomb bodies and nuclear inclusions constituted of inosine monophosphate dehydrogenase 2 (IMPDH2). First, we examined the relationship between MCM proteins and DNA replication. Using immunofluorescent labeling of cells extracted prior fixation and applying cross-correlation function analysis, we showed that MCM proteins are present at the sites of active DNA synthesis. Our results contributed to the solving of the first part of so-called MCM paradox. Second, we studied the structural basis of the Polycomb bodies. Based on fluorescence microscopy studies, Polycomb bodies have been considered to be the nuclear subcompartments formed by the accumulation of Polycomb proteins in the interchromatin compartment. In our work, using correlative light electron microscopy and experimental changes in macromolecular crowding, we clearly showed that a Polycomb body is a chromosomal domain formed by an accumulation of heterochromatin structures, rather than a typical nucleoplasmic body. Third, we were interested in...

The aim of the dissertation is to explain the military-political reasons behind the long-term attempt of the Soviet Union to deploy its units on Czechoslovak territory, as well as the root cause of the military operation against Czechoslovakia which was carried out by the five member armies of the Warsaw Pact. One of the main reasons for this was the military and strategic situation in Central Europe in the 1960's, and also the issue of nuclear weapons and their carriers. A separate chapter is dedicated to Soviet attempts to deploy its units on Czechoslovak territory prior to Operation Danube. The final chapter deals with the reorganization of the CPA, the establishment of the CGV and the NATO reaction to the change in the strenght of armed forces present on the border of the two Blocs.

This Master's thesis dealt with securitization attempts related to the planned construction of a new reactor at the Dukovany NPP. Therefore, its aim was to find, analyse and explain securitization speech acts associated with the planned reactor, in the period from summer 2015 to December 2020 using the critical discourse analysis approach. The results show that securitization speech acts of this kind do appear in the Czech public sphere and are articulated mainly by four groups of actors: representatives of Austria, Czech environmental NGOs, Czech governmental representatives, and Czech political opposition. These actors have different goals: to stop the construction of the reactor, to implement the construction of the reactor, or to influence the way in which the construction will be implemented. One case of successful securitization was found in the outcome: The Czech government was able to enforce extraordinary measures that deviated from standard democratic processes using security-based arguments. Their goal was to implement the construction of the reactor. It remains to add that the research also revealed three important functional actors who co-created the discourse: the BIS secret service, the SÚJB nuclear safety office and the company ČEZ.

This master’s thesis deals with usage of burn-up (spent) nuclear fuel in nuclear power plants of next generation – accelerator driven transmutation plants which is produced in current nuclear power plants. This system could significantly reduce the volume of dangerous long-lived radioisotopes and moreover they would be able to take advantage of its great energy potential due to fast neutron spectrum. In the introduction are listed basic knowledge and aspects of spent nuclear fuel along with its reprocessing and the possibility of further use while minimizing environmental impact. As another point detailed description of accelerator driven systems is described together with its basic components. In addition this search is followed by individual chronological enumeration of projects of global significance concerning their current development. Emphasis is placed on SAD and MYRRHA projects, which are used like base for calculations. This last, computational part, deals with the creation of the geometry of subcritical transmutation reactor driven by accelerator and subsequent evaluation which assembly is the most effective for transmutation and energy purposes along with changing of target, nuclear fuel and coolant/moderator.