Typescript (photocopy). / Digitized by Kansas State University Libraries

Digitized by Kansas State University Libraries

The Lincoln-Oseretsky Motor Development Scale is designed to test the motor ability of children between the ages of six and fourteen. It is an individually administered scale consisting of thirty-six motor skill items such as finger dexterity, eye-hand coordination and gross activity of the hands, arms, legs and trunk. Both unilateral and bilateral motor tasks are involved in the scale. Because of this fact, fifty-three test items involve right and left limb performances. The time for administering all the items is usually less than one hour. The purpose of this study was to determine whether or not the number of test items could be significantly reduced so that a reliable assessment or the motor ability of an individual can be made in a much shorter period of time.

It was determined spectrally that a monomer-dimer equilibrium exists in aqueous solutions of Ni-TSPC4- over a concentration range of 5 x 10-8 – 10-4 M. Equilibrium measurements were made under natural ionic strength conditions at 29°, 39° and 49°C. The values obtained for the equilibrium constants at 29°, 39° and 49°C were 2.4 x 108, 6.6 x 107 and 2.9 x 107 M-1, respectively. These results indicated that the equilibrium strongly favors dimer formation. This study also showed that nickel is second to copper in enhancing the dimerization of metallotetrasulfophthalocyanine complexes. However, metals in general inhibit dimer formation relative to the free ligand. The dimerization reaction of Ni-TSPC4- is exothermic with thermodynamic parameters of ΔG° = -11.6 kcal/mole, ΔH = -20.9 kcal/mole, and ΔS = -31 e.u. The dimerization process is thus favored from an energetic point of view despite the unfacorable entropy change. Spectral measurements made on Ni-TSPC4- in aqueous pyridine solutions in the concentration range of .05 - 6.0% pyridine (by volume) indicated a dipyridinated dimer complex of Ni-TSPC4- was formed. Using a modified version of the Benesi-Hildebrand equation, the following equilibrium was proposed : D + 2Py = D(Py)2. The overall equilibrium constants for this system at 15°, 21°, 30° and 40°C were 1.7, 1.9, 2.8, 3.6 and 4.4 M-2, respectively. The equilibrium reaction is endothermic with thermodynamic parameters of: ΔG=6.2 kcal/mole, ΔH =6.9 kcal/mole, and ΔS=25 e.u. Thus, the equilibrium reaction was favored thermodynamically due to the favorable entropy change associated with the process. Ligand studies indicated that only pyridine and polupyridine ligands bind to Ni-TSPC4- and cause a spectral change. This study also showed that Ni-TSPC4- cannot be used as an oxygen carrier in biological systems.

Some properties of esterases of the greater wax moth larvae, Galleria mellonella (L.) were examined and the enzyme partially purified. Tris buffer was chosen as the buffer system over veronal buffer. The former causes less hydrolysis of the acetylsalicylic acid. Esterase activities were determined with acetylasalicylic acid in tris buffer (00.05 M, pH 7.85 with 0.1 M EDTA) and with acetyl-p-hydroxybenzoic acid in the same buffer. The enzyme was partially purified by combination of acetone powder preparation, ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration. The starting enzyme preparation was obtained from aceton powder preparation. The ratio of esterase activity to protein content was increased 1.36-fold by 70% ammonium sulfate precipitation. The ammonium sulfate in the 60-70% saturation range is reproducible with the highest specific activity at 60% saturation. The ammonium sulfate fractionation increased the stability of the enzyme preparation. After 24 hours more than 55% of enzyme activity from the ammonium sulfate precipitate still remained, whereas only about 40% of the enzyme activity from the acetone powder preparation remained. The pH optimum was found to be 8.5 with acetyl-p-hydroxybenzoic acid as the substrate. The esterase had a linear increase in activity with increasing temperature. At 51°C, the specific activity was 27.60 micromoles/min mg protein in comparison to 12.94 micromoles/min mg protein at 20°C. The kinetic behavior of the enzyme with the different substrates was studied. The Vmax and Km values were 12.5 micromoles/min mg protein and 0.435 millimoles, respectively. The energy of activation with acetyl-p-hydroxybenzoic acid was 4.542 kcal K-1 mol-1. The esterase activity was inhibited by mercuric chloride and veratrine sulfate. It was activated by the chelating agent, EDTA. The esterase activity was not affected by p-chloromercuribenzoic acid and eserine salicylate.

o-CH3, o-CH3O, m-CH3O and p-CF3-β-methyl- β-nitrostyrene (compounds not previously reported) were synthesized using the method of Lappin13 and Robertson.14 These styrenes were irradiated in the U.V. region. The yield of corresponding oximes varied from 85 to 100% in aqueous ethanol. This variation in yield of the oximes was attributed to the effect of the substituent on the phenyl rings of the corresponding styrenes. Electron donating substituents promoted the nitro-nitrite rearrangement and electron-withdrawing groups inhibited this rearrangement. A mechanism is proposed for this reaction which unifies and includes ideas proposed by Chapman2, Pinhey5 and Matsurra.3 The effect of solvent on the irradiation was also studied. The results obtained indicated that the solvent employed does have an effect on the photorearrangement of beta-nitrostyrenes.

The activity of Catalyst 3-7A toward the oxidation of formaldehyde, hydrogen cyanide, methane, propane, and isobutene was examined at temperatures between 25°C and 400°C. The catalyst consists of palladium(II) and copper (II) salts impregnated on an alumina support. The oxidation measurements were made primarily with gas chromatographs, although form aldehyde and hydrogen cyanide were also examined using the colorimetric fushsin method and Liebig titrations, respectively. Catalyst 3-7A demonstrated appreciable activity toward the oxidation of all gases studied except formaldehyde where there was no evidence of oxidation. The latter result is quite unexpected since aldehydes are more easily oxidized than alkanes. It is suggested that the inactivity of Catalyst 3-7A toward formaldehyde is attributed to the lack of moisture which is required by the catalyst during oxidation. All oxidation reactions are believed to occur via a Qacker-type process under pseudo-first order conditions with the palladium(II) concentration maintained constant via the presence of copper (II) which oxidizes metallic palladium, the product in the oxidation reaction. The oxidation products in all the gases examined are believed to be carbon dioxide and water except for hydrogen cyanide where an additional product containing nitrogen should be formed but could not be detected with the gas chromatograph. Rate constants (reported in parenthesis) at 400°C for the following gases are hydrogen cyanide (>26.2 sec-1), methane (6.5-6.6 sec-1), propane (>12.8 sec-1), and isobutene (>15.7 sec-1). These results show that hydrogen cyanide is the most active gas to be oxidized by the catalyst excluding carbon monoxide which is the test gas used I our system and has been previously determined to demonstrate the greatest activity with the catalyst. The three alkane gases show an order of oxidation reactivity which lends some support for ruling out an anionic mechanism since tertiary C-H bonds were found to be more reactive than secondary C-H bonds which in turn were shown to be more reactive than primary C-H bonds. A comparison of Catalyst 3-7A with other catalysts reported in the literature which show similar oxidation behavior with gases could not be made due to lack of data regarding their experimental conditions.

The school budgets of the different school districts of the state of Kentucky are prepared according to law and by the standard plan adopted by the State Board of Education. Kentucky School Law 160.170 demands that each board of education prepare a budget and present it to the State Board of Education for approval. The standard form adopted by the State Board of Education contains the following items or main divisions: General Control, Instruction, Operation of School Plant, Maintenance, Fixed Charges, Auxiliary Service, Transfer of Tuition, Capital Outlay and Debt Services. This study will take the budget of each board of education and divide the amount spent in each division listed above by the census figure of the district and thus find the per capita for each division as well as the total per capita spent on each child in the census. Further, the amount budgeted in each division will be divided by the total amount budgeted to find the per cent spent for each division. The Kentucky Laws and the plan adopted by the State Board of Education do not recommend any certain amount to be spent per capita in each division no do they recommend any certain per cent for each division. This study shows that there is a wide variance among the different districts as to the per capita and the per cent spent for the different items of the budget. It is hoped that this study will make it possible for each superintendent and board of education to see at a glance how their school district compares with any other district in the state. It is not the purpose of the study to find out why so many variances occur, but to show a true picture and to make some recommendations that may be of benefit.

The Star Fire Coal Mine is a large strip mining operation (10,000 acre permit) that produces mine spoil consisting of sandstones, shales, siltstones, and underclays of the Pennsylvanian Breathitt Formation. Chemical analysis of groundwater from the saturated mine spoil show unusually high magnesium concentrations, with magnesium constituting between 27 and 47 percent of the major cations. Excess magnesium in water is detrimental to plant metabolism, and its content in groundwater may limit its use for irrigation and other uses during a post-mining land use plan. Petrological methods (thin section point count analysis and x-ray diffraction) and geochemical methods (electron microprobe analysis and water extraction experiments) were performed to determine the source of magnesium in sandstone and shale samples of the Breathitt Formation. Based on mineral percents and concentrations of magnesium found in samples used for waterrock interaction samples, magnesium-rich siderite was found as the primary contributor of magnesium to the groundwater. / Department of Geology

Thesis (Ph. D.)--Ohio State University, 1982. / Includes vita. Includes bibliographical references (leaves 379-412). Available online via OhioLINK's ETD Center.